Probe For Detection Of Hydrazine Research Articles

A new fluorescent probe for detection of hydrazine and extremely acidic pH in different modes

Toxic hydrazine poses a huge threat to living organisms and our environment. Extremely acidic pH is also harmful to various ecosystems. Here, a coumarin-based dye called CMM has been synthesized for the ratiometric detection of hydrazine (F504 nm/F560 nm). CMM could detect gaseous hydrazine in the environment and endogenous hydrazine in live cells. Furthermore, the fluorescence at 563 nm of CMM was stable from pH 10.0 to 2.0, whereas increased greatly from pH 2.0 to 1.2. Thus, the probe could also detect extremely acidic pH with great fluorescence enhancement. The pKa value was determined to be as low as 1.41. We anticipate that the probe could provide a good candidate for detection of toxic hydrazine and extremely acidic pH.

A Fluorescent Probe for Hydrazine Based on 4-hydroxycoumarin with High Selectivity and Sensitivity

Background: Therefore, the development of reliable analytical techniques with high selectivity and sensitivity to detect hydrazine is required for the protection of human health and safety. Objectives: Traditional methods for detecting N2H4 are frequently time-consuming, less accurate, and unsuitable for the analysis of living systems. Numerous fluorescent probes for hydrazine have been produced and gained some valuable results recently. The creation of a simple fluorescent probe for hydrazine detection is the goal of this project Method: In this study, 300 µL of probe 3-methyl-2-oxo-2H-chromen-7-yl propionate (MOCP) was mixed with an equivalent amount of the solution of each analyte to obtain the measurement solution. Following a 10-minute room temperature incubation period, the fluorescence spectra of the resultant solution were recorded. Results: The fluorescence intensity of the probe was noticeably enhanced when N2H4 was added to the probe, but almost no fluorescence enhancement was observed when other competitive ions were added. Conclusion: A hydrazine fluorescent probe based on 4-hydroxycoumarin fluorophore was developed. The probe MOCP displayed high sensitivity and selectivity for hydrazine, with a color change from colourless to blue for detection by the naked eye. Moreover, it demonstrated a low detection limit of 20 nM and a fast reaction time of 30 s.

Naphthalene-based fluorescent probe for on-site detection of hydrazine in the environment.

The widespread occurrence of hydrazine residues in the environment, including in water, soil, and organisms, is a potential health threat to humans. Therefore, the development of an efficient method for the detection of hydrazine in environmental samples is highly desirable although it poses a significant challenge. In this study, we designed and synthesized a series of naphthalene-based fluorescent dyes through structural engineering and developed a novel probe for hydrazine detection. The probe could provide a distinct fluorescence response toward hydrazine in aqueous solution with high sensitivity and selectivity. Moreover, paper-based test strips can be easily fabricated using this probe, enabling the portable on-site detection of hydrazine with the aid of a smartphone. Furthermore, we demonstrated that this probe is capable of recognizing hydrazine in various environmental samples, including water, soil, plants, and zebrafish embryos. This research provides a promising tool for the detection of hydrazine in the environment.

Coumarin functionalized cellulose-based fluorescent probe for detection of hydrazine and its applications in environmental analysis

Hydrazine is a kind of widely used chemical reagent, which has the harmfulness to human health and ecosystem. Therefore, it is necessary to develop a rapid and sensitive method for the detection of hydrazine. In this work, a novel cellulose-based fluorescent probe (ACBC-CA) was rationally developed for the selective and sensitive detection of hydrazine. ACBC-CA was synthesized by using cellulose acetate as skeleton and coumarin-based derivative as the fluorophore. ACBC-CA exhibited a significant “turn-on” fluorescence response to hydrazine, accompanied by a distinct fluorescence color change from colorless to bright yellow. The detection limit of ACBC-CA for hydrazine was calculated to be 1.2 × 10−7 M. Furthermore, ACBC-CA was successfully applied to determine hydrazine in real environmental water and soil samples.

A Graphene Oxide-based Covalent Resorufin-Conjugated Fluorescence "OFF-ON" Probe for Detection of Hydrazine.

The unique properties of graphene oxide (GO), such as good water solubility, non-toxicity, biocompatibility and cell permeability, make GO well suited for a number of biological applications. In this study, we explore the application of GO as an efficient fluorescent quencher for a highly fluorescent organic dye, resorufin (RF). The quenching effect of GO on resorufin is studied by noncovalent and covalent bonding of resorufin, respectively. The fluorescence is completely quenched when resorufin is covalently linked to GO; while resorufin's fluorescence could be still observed through noncovalent bonding to GO sheet. Interestingly, addition of hydrazine into RF-GO complex causes the fluorescence recovered, providing a potential ''OFF-ON'' fluorescence responsive probe for detecting hydrazine in vitro and in living cells.

A new phenothiazine-based fluorescent probe for detection of hydrazine with naked-eye color change properties

A new phenothiazine-based fluorescent probe for detection of hydrazine with naked-eye color change properties

Development of Imidazo[1,2-a]pyridine-based probe for detection of hydrazine and its applications in imaging of HepG2 cell

A novel fluorescent probe IMPD based on imidazo[1,2-a]pyridine for detection of hydrazine was designed and synthesized. This probe used maleimide as the recognition group, which was rapid and sensitive for hydrazine. To compare with our previous probe for hydrazine, this newly probe showed a lower detection limit for 9.1 ppb. In addition, the probe IMPD could dye the HepG2 cell with blue color at the presence of hydrazine.

A highly sensitive fluorescent probe for hydrazine detection: synthesis, characterisation and application in living cells

ABSTRACT A new fluorescent probe HSL-AI derived from imidazo [1,5- a]pyridine was synthesised for the detection of hydrazine (N2H4) based on excited-state intramolecular proton transfer (ESIPT). HSL-AI was designed using acetyl group as the recognition site and exhibited high selectivity towards N2H4 with a low limit of detection (2.56 ppb). Moreover, HSL-AI was successfully applied to detect N2H4 in living cells.

A merocyanine-based dual-mode optical probe for detection of hydrazine and its bioimaging application invitro and vivo.

In this paper, a Merocyanine-based turn-on probe (McyA) has been developed for colorimetric and fluorescent dual-mode detection of N2H4 via an intra-molecular charge transfer (ICT) mechanism. In the presence of N2H4, the probe shows an obvious chromogenic response and fluorescent enhancement. Based on this feature, the synthesized McyA can be applied to quantify N2H4 concentration from 0.12 to 5.0 μM and 0.5-10 μM with a detection limit of 0.042 μM (1.3 ppb, S/N = 3) and 0.140 μM (4.5 ppb, S/N = 3), respectively. Moreover, McyA has also be successfully employed for imaging analysis of N2H4 distribution in different organs from the N2H4 ingested mice model, revealing the potential application of McyA as a powerful fluorescent sensor for tracking detection and risk assessment of hydrazine invivo.

A New HBT-Based Fluorescent Probe for Hydrazine Detection in Aqueous Solution

A New HBT-Based Fluorescent Probe for Hydrazine Detection in Aqueous Solution

Benzthiazole-derived chromogenic, fluorogenic and ratiometric probes for detection of hydrazine in environmental samples and living cells

Abstract Benzothiazole-based chromogenic and ratiometric fluorescent chemodosimetric probes CD1 and CD2, were synthesized and characterized. The probes are designed in such a way that the excited state intramolecular proton transfer (ESIPT) of the benzothiazole moiety gets blocked. Upon treatment with hydrazine in organo-aqueous medium[DMSO: H2O; 2:1 (v/v)] at physiological pH, the aectyl protective group of probes CD1 and CD2 were removed readily in presence hydrazine and ESIPT of the probes were switched on, which resulted remarkable photo-physical changes. These two ESIPT–based ratiometric fluorescent probes were shown to be selective and sensitive for hydrazine among different cations, anions and amines studied in organo-aqueous medium[DMSO: H2O; 2:1 (v/v)] at physiological pH, by fluorescence, absorption, and visual emission color change. These key features allows the two probes to be employed for hydrazine detection by simple visual inspection. DFT and TDDFT calculations were performed in order to demonstrate the sensing mechanism and the electronic properties of probe and hydrazinolysis product. An easy-to-prepare test strips, obtained by dipping the TLC plates into the solution of CD1 and CD2, were able to detect hydrazine in practical samples. Moreover, the utility of the probes CD1 in showing the hydrazine recognition in live cells has also been demonstrated using Vero cells as monitored by fluorescence imaging.

A novel pyrazoline-based fluorescent probe for detection of hydrazine in aqueous solution and gas state and its imaging in living cells

A novel fluorescent probe 4 for hydrazine based on pyrazoline-derived was designed and synthesized. The selective deprotection of phthalimide group of the probe by hydrazine led to a dramatic fluorescent enhancement (∼84.7 folds) at 490nm within 4min in the aqueous solution. The limit of detection is 0.0622μM (1.99ppb) which is much lower than the U.S. Environmental Protection Agency standard (10ppb). Notably, probe 4 could be used to detect hydrazine in gas state with different concentrations through color changes from readily prepared strips. Additionally, probe 4 could also be applied to monitor hydrazine in living cells by biological imaging.

A highly sensitive fluorescent probe for detection of hydrazine in gas and solution phases based on the Gabriel mechanism and its bioimaging

A new probe 2-benzo[1,2,5]thiadiazol-4-yl-isoindole-1,3-dione (BTI) based on the Gabriel reaction was developed for hydrazine which shows promising selectivity towards hydrazine over other analytes.

A charge transfer based ratiometric fluorescent probe for detection of hydrazine in aqueous medium and living cells

An intramolecular charge transfer (ICT) based fluorescent probe 2 has been designed and synthesized for the ratiometric detection of hydrazine in PC3 cell lines. Further, probe 2 demonstrates the selective naked-eye detection of hydrazine in the presence of representative metal ions in an aqueous environment. Further, to promote the selective, economic and easy detection of hydrazine, the fluorescent test strips of probe 2 have been prepared which can detect hydrazine up to femtogram level.

A facile intracellular fluorescent probe for detection of hydrazine and its application

A highly selective fluorescent probe for detection of hydrazine exhibiting good performance in a physiological environment has been developed over other closely related analytes. Furthermore, hydrazine in diluted human blood was tested and the method was applied in live cells.