A highly ultrafast and sensitive electrochemical sensor based on electrical potential-assisted hybridization was developed for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The electrode was modified with multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) for excellent electrochemical performance. The constant electric field was applied to rapidly and efficiently enrich the capture probes to the electrode surface in 60 s. Meanwhile, pyrene as the backfill reagent resulted in a significant reduction of nonspecific adsorption. The electrochemical properties of the modified electrode were investigated via electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The analytical response was measured by differential pulse voltammetry (DPV). The sensor achieved the ultrafast hybridization of nucleic acids at the electrode interface within 90 s, substantially reducing the reaction time from hours to minutes. Under optimal conditions, the sensor exhibited a superior detection limit of 60 fM and showed satisfactory specificity toward single-base mismatch. Additionally, the proposed sensor realized sensitive determination of SARS-CoV-2 in throat swabs. The enzyme-free and non-amplification sensing strategy showed promising potential for application in point-of-care testing (POCT).