This research elucidates the changing function of some of the earliest types of monumental architecture in the Maya world, the E-Group, using ICP-MS soil chemistry analysis of associated floor surfaces at the site of Holtun, Guatemala. E-groups are architectural assemblages first appearing during the Middle Preclassic period that are associated with public, ritual activities, such as marking important celestial and agricultural events. Our methodology targeted sequential, stratigraphic plaza floors in the E-Group compound at Holtun to examine changing activities through time. Residential patio surfaces were also sampled as a comparison to public, ritual activity. Our results suggest that activities related to food production and consumption are visible in sequential plaza floors of the E-Group, and the locations of some of these activities changed over time. Moreover, activities and locations revealed through soil chemical analysis also differed somewhat from those in residential patios, suggesting different patterns of use in public versus private spaces.