Objective: To determine the association of Fecal Calprotectin (f-CP) and M2 Pyruvate Kinase (M2-PK) with intestinal pathogens by means of the gastrointestinal syndromic panel. Methodology: This retrospective analytic study was conducted in the laboratory department a private hospital in Jakarta from October 2021 to September 2022. The inclusion criteria comprised patients aged ≥18 years with gastrointestinal complaints who had undergone f-CP and M2-PK examinations and gastrointestinal syndromic testing. The exclusion criteria were incomplete data on laboratory examinations, follow-up examinations or treatment evaluations. We used the Mann-Whitney test with p<0.05 indicating significantly different. Results: Out of 282 subjects, 69% were women and 31% men, aged 18 to 84 years (mean 50.52±15.20). In 117 subjects, single pathogen was found in 78 specimens and ≥2 pathogens in 39. Median f-CP was 77.1 µg/g (3.68-2100) and median M2-PK 3.55 U/mL (1-72.6). Mann-Whitney test found a significant association between f-CP and intestinal pathogens (p=0.048), whereas for the M2-PK, no significant association was found with intestinal pathogens a(p=0.381). Conclusion: f-CP increases in gastrointestinal infection with intestinal pathogens, but M2-PK does not increase. Therefore, f-CP can be considered a biomarker for gastrointestinal infections caused by microorganisms.
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