Soil particle distribution is an important soil property that is widely studied. However, more attention has been focused on specific environmental factors. As soil and environmental factors of nature are related, what is the relationship between soil particle distribution and environmental factors? What are the most important factors affecting soil particle distribution at the watershed scale? The principal component dimension reduction analysis showed that the important factors affecting soil particle distribution included and ranked as parent material, slope and slope position. The karst soil in the Houzhaihe Basin, Guizhou Province, China, is taken as the research object. A grid of 150 m·150 m is used for sampling, and the total number of sample collection points is 2755. The effects of seven environmental factors (soil parent material, land use type, slope direction, slope, soil type, slope position and altitude) on soil particle distribution were studied. The results showed that the seven factors all significantly affected the soil particle distribution in the basin, but the significance of different factors was distinct, and some factors had no significant effect on the specific particle type. The principal component dimension reduction analysis showed that the important factors included parent material, slope and slope position, which were ranked as parent material, slope and slope position. On the one hand, the change in soil particle distribution is affected by the factors of the soil formation process (parent material) and by the transport and transportation of dynamic factors such as water and gravity (slope and slope position) in the basin. The change in soil particle distribution is driven by the coupling of these two factors. The redundancy analysis shows that the contribution rates of environmental factors to the content of three kinds of soil particle sizes are arranged in the order of soil parent material, land use type, slope direction, slope, soil type, slope position and altitude and explained 14.2 %, 8.9 %, 3.4 %, 3.0 %, 1.9 %, 1.5 % and 0.9 %, respectively, and only three of them reach the level of P < 0.05. The results of high-density sampling analysis at the karst basin scale show that the parent material is the most important factor affecting the soil particle distribution.