Growth Of Primary Producers Research Articles

Photosynthetic light requirement near the theoretical minimum detected in Arctic microalgae

Photosynthesis is one of the most important biological processes on Earth, providing the main source of bioavailable energy, carbon, and oxygen via the use of sunlight. Despite this importance, the minimum light level sustaining photosynthesis and net growth of primary producers in the global ocean is still unknown. Here, we present measurements from the MOSAiC field campaign in the central Arctic Ocean that reveal the resumption of photosynthetic growth and algal biomass buildup under the ice pack at a daily average irradiance of not more than 0.04 ± 0.02 µmol photons m−2 s−1 in late March. This is at least one order of magnitude lower than previous estimates (0.3–5 µmol photons m−2 s−1) and near the theoretical minimum light requirement of photosynthesis (0.01 µmol photons m−2 s−1). Our findings are based on measurements of the temporal development of the under-ice light field and concurrent measurements of both chlorophyll a concentrations and potential net primary production underneath the sea ice at 86 °N. Such low light requirements suggest that euphotic zones where photosynthesis can occur in the world’s oceans may extend further in depth and time, with major implications for global productivity estimates.

Global Vegetation‐Temperature Sensitivity and Its Driving Forces in the 21st Century

AbstractIt has been projected that climatic warming will contribute to vegetation productivity variability at the global scale. With a continued warming, to what extent and where the vegetation productivity is most affected by warming has still not been adequately quantified. Herein, based on 11 earth system model outputs, we predict the characteristics of vegetation‐temperature sensitivity (Svpt, defined as higher/lower temperature produce more/less vegetation) changes under different CO2 emission scenarios and various vegetation types, further assessing the relationship of the Svpt to socio‐ecosystems. At the end of the 21st century, the area proportion with the global temperature increases >2°C under the SSP1‐2.6, SSP2‐4.5, SSP3‐7.0, and SSP5‐8.5 scenarios are 3%, 40%, 97%, and 99%, respectively. The largest contribution to the global terrestrial gross primary productivity growth is at low latitudes. 33%–63% of global terrestrial ecosystems show a significantly negative trend in the Svpt, indicating the weakened promotion effect of warming on vegetation growth. In particular, in 2060, there will be a clear reversal of the trend from carbon sink to carbon source under the SSP3‐7.0 scenario, spatially distributed mainly in the Amazon rainforest, tropical Africa, and southern North America. Precipitation is also an important factor affecting the Svpt change, and there is an inverted U‐shaped relationship between them. Precipitation thresholds are higher under the high emission scenario when Svpt reaches its highest value. Moreover, socio‐demographic pressures in places like Central Africa and East Africa will offset the promotion of vegetation growth by warming; in the future, these countries should develop appropriate population and land management strategies to achieve socio‐ecosystems sustainable development.

Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy

To accelerate the depletion of total petroleum hydrocarbons, a hydrocarburoclastic ascomycetes, Lambertella sp. MUT 5852, was bioaugmented to dredged sediments co-composting with a lignocellulosic matrix. After only 28 days of incubation, a complete depletion of the contamination was observed. The 16S rDNA metabarcoding of the bacterial community and a predictive functional metagenomic analysis were adopted to evaluate potential bacterial degrading and detoxifying functions. A combination of toxicological assays on two eukaryotic models, the root tips of Vicia faba and the human intestinal epithelial Caco-2 cells, was adopted to assess the robustness of the process not only for the decontamination but also for the detoxification of the dredged sediments. Bacterial taxa, such as Kocuria and Sphingobacterium sps., resulted to be involved in both the decontamination and detoxification of the co-composting dredged sediments by potential activation of diverse oxidative processes. At the same time, the Kocuria sp. showed plant growth-promoting activity by the potential expression of the 1-aminocyclopropane-1-carboxylate deaminase activity, providing functional traits of interest for a technosol in terms of sustaining primary producer growth and development.

PCO2 decrement through alkalinity enhancement and biological production in a shallow-water ecosystem constructed using steelmaking slag

Ocean-based carbon dioxide removal has gained immense attention as a countermeasure against climate change. The enhancement of ocean alkalinity and the creation of new blue carbon ecosystems are considered effective approaches for this. To evaluate the function of steelmaking slag from the viewpoints of CO2 reduction and creation of new blue carbon ecosystems, we conducted a comparative experiment using two mesocosms that replicated tidal-flats and shallow-water ecosystems. Initially, approximately 20 seagrasses (Zostera marina) were transplanted into the shallow-water area in the mesocosm tanks. The use of steelmaking slag is expected to increase the pH by releasing calcium and mitigate turbidity by solidifying dredged soil. In the experimental tank, where dredged soil and steelmaking slag were utilized as bed materials, the pH remained higher throughout the experimental period compared with the control tank, which utilized only dredged soil. As a result, pCO2 remained consistently lower in the experimental tank due to mainly its alkaline effect (March 2019: −10 ± 6 μatm, September 2019: −130 ± 47 μatm). The light environment in the control tank deteriorated due to high turbidity, whereas the turbidity in the experimental tank remained low throughout the year. The number of seagrass shoots in the experimental tank was consistently approximately 20, which was higher than that in the control tank. Additionally, more seaweed and benthic algae were observed in the experimental tank, indicating that it was more conducive to the growth of primary producers. In conclusion, tidal-flat and shallow-water ecosystems constructed using dredged soil and steelmaking slag are expected to enhance CO2 uptake and provide a habitat for primary producers that is superior to those constructed using dredged soil only.

Use of decomposition products of Pontederia crassipes Mart., Solms (water yacinth) for inhibition of cyanobacteria growth: A full-scale study

Eutrophication exacerbates the growth of primary producers that may reduce water quality, such as macrophytes and cyanobacteria. The floating macrophyte Pontederia crassipes and the cyanobacterium Raphidiopsis raciborskii occur worldwide in eutrophic environments, but data on the inhibition of R. raciborskii by floating macrophytes are scarce. We aimed to test the inhibitory activity of P. crassipes on cyanobacterial growth, especially R. raciborskii. First, we tested the effect of live P. crassipes exudates and decomposing macrophyte extracts on a R. raciborskii strain under culture conditions. Cyanobacterial growth was not affected by the exudate from the live macrophyte. In contrast, exposure to decaying macrophyte extracts containing 4.0 and 6.0 mg L−1 total phenols completely inhibited cyanobacterial growth after five days and decreased photosynthetic activity. Mass spectrometry FT-IRC analysis led to the identification of some molecules in P. crassipes extracts, but no association with the inhibitory effect of the was identified. We then tested the inhibitory effect of P. crassipes in mesocosms containing a natural cyanobacterial bloom. The slow release of chemicals from the dry biomass over 12 days decreased chlorophyll concentrations. Finally, in a field experiment, we applied tons of P. crassipes dry biomass to large areas (900 m3) of a reservoir to test the release of chemicals into the water during one month. At the final time, the phenol concentration in the backwater region was close to the inhibitory value found for cyanobacterial growth and an inverse correlation was observed between chlorophyll and phenol concentrations. Thus, under natural conditions, the release of phenolic compounds and other allelochemicals from the decomposing material of macrophyte mats inhibits cyanobacterial growth. Macrophyte biomass, which is usually considered waste, might be used to mitigate cyanobacterial blooms and may be a promising new and sustainable biotechnological tool to improve water quality.

Nitrate and phosphate profiles in the Banda Sea during the northwest monsoon

The Banda Sea (BS) is subjected to monsoonal winds that affected upwelled rich nutrient waters and primary production. However, nutrient characteristic in the region is less constrained. Here, we examine nitrate and phosphate profiles in the Banda Sea from data collected during the northwest monsoon. Waters along the surface density (σθ ) < 22 were dominated by less saline originating from western Indonesia (i. e., Java Sea), accompanied by Indonesian throughflow water Banda (23.0-25.0σθ ) and Banda water (26.5-27.0σθ ), respectively. Surface water was accompanied by low nitrite+nitrate () and phosphate (), meanwhile, the average and concentrations in the upper 500 m were slightly higher in the western part than those in the eastern part suggesting additional sources from upwelled waters. Furthermore, the average nitrite+nitrate to phosphate ratio (N:P ratio) was 11.2:1, indicating nitrogen is a limiting factor for the growth of primary producers, and other sources may play a more dominant role. Two distinct layers in the euphotic zone within different sources and mechanisms namely the nutrient-depleted layer and nutrient-repleted layer were observed. Overall, the result of this study can only be seen as a starting point toward a comprehensive and holistic understanding of the nutrient biogeochemistry of the Indonesia Seas.

Biofouling of a unionid mussel by dreissenid mussels in nearshore zones of the Great Lakes.

In North America, native unionid mussels are imperiled due to factors such as habitat degradation, pollution, and invasive species. One of the most substantial threats is that posed by dreissenid mussels, which are invasive mussels that attach to hard substrates including unionid shells and can restrict movement and feeding of unionids. This dreissenid mussel biofouling of unionids varies spatially in large ecosystems, such as the Great Lakes, with some areas having low enough biofouling to form effective refugia where unionid mussels might persist. Here, we measured biofouling on mussels suspended in cages over the growing season (generally first week in June to last week of August) over 3 years in nearshore areas in Lake Erie (2014-2016), Lake Michigan (Grand Traverse Bay, 2015 and Green Bay, 2016), and Lake Huron (2015). Biofouling varied substantially by years within Lake Erie, with increasingly higher biofouling rates each year. Although dreissenid mussels are present throughout these lakes, we observed very low biofouling in Grand Traverse Bay (Lake Michigan) and Saginaw Bay (Lake Huron), with no dreissenid mussels in 8 of 9 sites across these two bays. Sampling in the rivermouth of the Fox River (Wisconsin) and the Maumee River (Ohio) both showed very high biofouling in areas adjacent to the outlet of these tributaries into Green Bay and Maumee Bay (Lake Erie), respectively. These watersheds are dominated by agriculture, and we would expect high growth of primary producers (i.e., mussel food) and primary consumers (unionids and zebra mussels) in these areas compared to the other sampled bays or the open waters of the Great Lakes.

Resolving the Drivers of Algal Nutrient Limitation from Boreal to Arctic Lakes and Streams

Nutrient inputs to northern freshwaters are changing, potentially altering aquatic ecosystem functioning through effects on primary producers. Yet, while primary producer growth is sensitive to nutrient supply, it is also constrained by a suite of other factors, including light and temperature, which may play varying roles across stream and lake habitats. Here, we use bioassay results from 89 lakes and streams spanning northern boreal to Arctic Sweden to test for differences in nutrient limitation status of algal biomass along gradients in colored dissolved organic carbon (DOC), water temperature, and nutrient concentrations, and to ask whether there are distinct patterns and drivers between habitats. Single nitrogen (N) limitation or primary N-limitation with secondary phosphorus (P) limitation of algal biomass was the most common condition for streams and lakes. Average response to N-addition was a doubling in biomass; however, the degree of limitation was modulated by the distinct physical and chemical conditions in lakes versus streams and across boreal to Arctic regions. Overall, algal responses to N-addition were strongest at sites with low background concentrations of dissolved inorganic N. Low temperatures constrained biomass responses to added nutrients in lakes but had weaker effects on responses in streams. Further, DOC mediated the response of algal biomass to nutrient addition differently among lakes and streams. Stream responses were dampened at higher DOC, whereas lake responses to nutrient addition increased from low to moderate DOC but were depressed at high DOC. Our results suggest that future changes in nutrient availability, particularly N, will exert strong effects on the trophic state of northern freshwaters. However, we highlight important differences in the physical and chemical factors that shape algal responses to nutrient availability in different parts of aquatic networks, which will ultimately affect the integrated response of northern aquatic systems to ongoing environmental changes.

Response of nutrient limitation to invasive fish suppression: How carcasses and analog pellets alter periphyton

The native Yellowstone Cutthroat Trout (Oncorhynchus clarkii bouvieri Jordan and Gilbert, 1883) population in Yellowstone Lake, Yellowstone National Park, Wyoming, USA, is in decline because of competition from the introduced, invasive Lake Trout (Salvelinus namaycush Walbaum in Artedi, 1792). Gillnetting is used to suppress adult Lake Trout; however, methods are being developed to suppress embryos, including adding Lake Trout carcasses and carcass-analog pellets to spawning sites. Decomposing carcasses and analog pellets cause decreased dissolved oxygen concentrations thereby leading to Lake Trout embryo mortality, but the effects of these methods on primary producers are unknown. We deployed in-situ nutrient diffusing substrates (NDS) at 3 spawning sites. The 1st site was treated with carcasses, the 2nd site was treated with analog pellets, and a 3rd lacked treatment (control). To estimate how suppression measures may alter nutrient limitation, we measured algal biomass in 6 NDS amendments at each site: nothing (control), N, P, N + P, ground carcasses, or pulverized analog pellets. We deployed 5 replicates of each amendment at each site before and after treating whole sites. N and P co-limited periphyton before carcasses or analog pellets were added to spawning sites (p < 0.01); however, nutrients were not limiting after the treatments were added to spawning sites (p = 0.31–1). Algal biomass was 4× higher after whole-site carcass treatments. In contrast, analog pellets appeared to suppress algal biomass in the amendments (20% of NDS at the control site post-treatment) and in the treatment plot (33% of pre-treatment biomass at analog pellet site). We also measured how individual ingredients in analog pellets altered periphyton biomass, which suggested that vitamin E, estrogen, and soybean oil ingredients reduced the growth of primary producers. Suppression methods may stimulate or reduce algal biomass, depending on the methods used, which could have cascading effects on food webs and potentially reduce the success of the control measures. Estimating how different Lake Trout suppression methods may alter basal resources in the littoral zone of Yellowstone Lake will help natural resource agencies develop effective plans to control invasive predators at early life stages while minimally altering ecosystems.

Effect of Climatic Fluctuations on the Structure and Functioning of Ecosystems of Continental Water Bodies

Climatic fluctuations are among the most important factors that cause changes in terrestrial and aquatic ecosystems. This review considers the principal mechanisms of the influence of climate changes on the structure and functioning of ecosystems of water bodies and shows the need to take these mechanisms into account when developing strategies for conserving the biological resources of aquatic ecosystems. Climatic fluctuations affect aquatic ecosystems through changes in temperature, surface runoff of nutrients and other substances and their ratios, the intensity of water mixing during the circulation period, and other mechanisms. Additional nutrients received in rainy periods from the catchment area and directly with precipitation stimulate the growth of primary producers and cause the risk of the further eutrophication of water bodies. An increase in temperature promotes the growth of potentially toxic phytoplankton species and exacerbates the problem of green tides, the massive development of multicellular algae in the coastal zone. Organic substances coming from the catchment area during wet periods stimulate a microbial loop in aquatic ecosystems. In shallow lakes, climate fluctuations can cause changes in food webs and the ecological regime. Climate-induced changes in the composition of producer communities often weaken pelagic–benthic relationships in aquatic ecosystems. In some cases, climate changes have contributed to the invasions of alien species. The natural dynamics of ecosystems affected by climate fluctuations deserves close attention and requires the development of special adaptive management of aquatic biological resources. In some cases, it is necessary to take more severe measures for the protection and restoration of water bodies, which would take into account adverse changes in natural factors.

Macroalgal defense phenotype correlates with herbivore abundance

A primary goal in the study of producer–herbivore interactions is to characterize the tradeoffs between primary producer growth and defense. Across the Aleutian Island Archipelago, the widespread decline in sea otters has resulted in reduced predation on sea urchins, which has led to increases in urchin populations, the formation of urchin barrens, and ultimately to overgrazing of much of the region’s kelp forests. The occurrence of both kelp forests and urchin barrens on islands, along with among island variation in the time period that urchin barrens have formed, presents a unique opportunity to characterize the extent to which exposure to intense herbivory and increased light may alter marine macroalgal growth and defense tradeoffs. To address this, we used a field caging experiment with Codium ritteri, a common perennial green macroalga in the Aleutian Archipelago, to test whether urchin barren macroalgae exhibit increased defenses and reduced growth relative to kelp forest individuals. Our results suggest that urchin barren C. ritteri had greater defense than growth relative to kelp forest individuals. In the laboratory, we found little evidence for urchin barren C. ritteri growth under low light or altered defenses at high light. Grazing rates on C. ritteri were correlated with urchin biomass in the field suggesting higher herbivory intensity may shift primary producer energy allocation from growth to defense. Together, our data suggest that macroalgae occurring within kelp forests grow faster but are more palatable than macroalgae occurring in urchin barrens, which may increase urchin deforestation potential.

Biogeography of macrophyte productivity: Effects of oceanic and climatic regimes across spatiotemporal scales

AbstractSpatiotemporal variability in primary producer growth rates is a fundamental aspect of community structure. Understanding drivers of these patterns and their response to climate variability and change are ongoing challenges. Nutrient and light limitations often are invoked as proximate drivers of these patterns, but many other environmental and biological factors vary across spatial and temporal scales. In temperate rocky intertidal habitats, macrophytes are major space occupiers and the base of the food web, and thus their patterns of primary production relate directly to their functions and services in these communities. We investigated spatiotemporal patterns of the primary production of two species of macrophytes, the kelp Hedophyllum sessile and the surfgrass Phyllospadix scouleri, across 908 km of Oregon and California coastline. Spatiotemporal variability in macrophyte growth rates and their relationships to regional or local‐scale environmental variables (upwelling, nutrients, temperature, light, phytoplankton blooms) and climate regimes were explored. Paradoxically, we found that both warmer water temperature (e.g., warm phases of climate patterns, weaker upwelling) and increased nutrients (e.g., with stronger upwelling) increased macrophyte productivity. Kelp growth decreased with dense phytoplankton blooms, while surfgrass growth decreased with increasing air temperature. Growth rates reflected tissue elemental content in surfgrass but only weakly in kelp. Hence, as climate warms and/or if upwelling intensifies, productivity of these and perhaps other macrophytes should increase, at least until thermal conditions, particularly low tide air temperature, become too stressful.

Satellite-derived changes in floodplain productivity and freshwater habitats in northern Australia (1991–2019)

Abstract The ecological communities supported by freshwater habitats and wetlands that persist from floodplain inundation generate numerous cultural, recreational and economic values via commercial fisheries and other human uses of these habitats. However, the alteration of flow connectivity, degradation, and disruption of physical processes that sustain different levels of organisms (e.g., primary producers) are threats that affect aquatic biodiversity and the productivity of these habitats. Therefore, the large scale assessment of freshwater habitats for proactive water resources planning and the development of climate change mitigation and resource management strategies is essential. However, such monitoring and assessment is complicated by the inaccessibility of many large wetland systems during times of inundation, making in situ sampling unfeasible at a time when high levels of aquatic primary producers are generating food and energy sources for higher order consumers. To understand the physical dynamics of aquatic primary producers and freshwater habitats (1991–2019) during such times in a large floodplain river in northern Australia (Gilbert catchment), this study integrated Landsat-derived modified normalised difference water index and normalised difference vegetation index in a classification tree model. Thereafter, key hydrological drivers (rainfall and river discharge) of floodplain productivity and connectivity were assessed using a range of multivariate techniques. Results show that the floodplain had a high aquatic plant biomass during the summer wet season based on the correlation between aquatic biomass accumulation (hot spots) and inundation ( r = 0.83 @ phase lag r = 0.68 @ lag = 1 month) at downstream Gilbert catchment appear to be a relatively stronger indicator of hot spots of floodplain biomass accumulation as opposed to local rainfall ( r = 0.57 @ lag = 3 months). While the downstream discharge explains a significant proportion of variability in the leading orthogonal mode of rainfall ( r = 0.83 ), statistical relationships developed between discharge/rainfall and the distribution of aquatic primary producers confirm that both rivers (flow) and local rainfall are key optimal predictors of inundation and sites of primary producers with river flows being a better indicator of the latter. It is therefore argued that hydro-meteorological fluctuations will be key constraints on freshwater habitats and the growth of primary producers, albeit, human disturbance of flow can impact on floodplain productivity. As illustrated in this study, such constraints are reflected in the spatial patterns and changing characteristics (connectivity and spatial heterogeneity) of hot spots of primary producers and freshwater habitats (Palustrine, Lacustrine, and Riverine ecosystems).

Do leaf‐litter decomposers control biofilm primary production and benthic algal community structure in forest streams? Insights from an outdoor mesocosm experiment

Abstract 1. Forested headwater streams are generally considered to be light‐limited ecosystems where primary production is reduced, and the main source of energy and nutrients is composed of allochthonous detritus. We hypothesised that in these ecosystems, the development of primary producers might also be limited by (1) competition for nutrients with leaf‐litter decomposers (e.g. bacteria and fungi), and (2) leaf‐litter leachates or allelopathic compounds produced by aquatic fungi. 2. To test these hypotheses, a 48‐day mesocosm experiment was performed in 12 artificial streams containing stream water inoculated with epilithic biofilm suspensions collected from a forested headwater stream. Three different treatments were applied: control without leaf litter (C), microbially conditioned leaf litter added at the beginning of the experiment and left to decompose throughout the experiment (L), or leaf litter renewed three times during the experiment (RL). 3. We predicted that (1) the presence of litter, through microbial nutrient immobilisation and allelopathy, would reduce primary production and that (2) this effect would be amplified by litter renewal. We also predicted that nutrient competition would mean that (3) leaf‐litter decomposers will alter primary producer community composition and physiology. These predictions were tested by analysing biofilm development, physiology, stoichiometry, and benthic algal community structure. To distinguish between the effects of nutrient immobilisation and allelopathy, the biofilm responses to leaf‐litter leachates collected after different microbial conditioning durations were also measured in a parallel laboratory experiment. 4. Contrary to our expectations, by day 28, primary producer growth was higher in the mesocosms containing leaf litter (L and RL) despite the rapid decrease in dissolved nutrients when leaf litter was present. After 48 days, the lowest phototrophic biofilm development was observed when leaf litter was renewed (RL), whereas phototrophic biofilm development was similar in the C and L treatments. Biofilm stoichiometry indicated that this effect was most probably related to greater nitrogen limitation in the RL treatment. The presence of leaf litter also affected primary producers' photophysiology, which could be attributed to changes in taxonomic composition and to physiological adjustments of primary producers. 5. Laboratory measurements showed that despite a strong inhibition of primary producer growth by unconditioned leaf‐litter leachates, microbially conditioned leaf litter had either low or no effects on the development of primary producers. 6. These results reveal that leaf‐litter decomposers can have both positive and negative effects on primary producers underlining the need to consider microbial interactions when investigating the functioning of forested headwater streams.

Screening of microalgae and LED grow light spectra for effective removal of dissolved nutrients from cold-water recirculating aquaculture system (RAS) wastewater

Popularity of recirculating aquaculture systems (RAS) is increasing. Because of the high water recirculation rate, dissolved nutrients originating from fish feed are concentrated enough in RAS wastewater (WW) to enable growth of primary producers, e.g. microalgae. This study evaluated nitrate-nitrogen (NO3-N) and phosphate-phosphorus (PO4-P) removal efficiency of ten temperate zone freshwater microalgae species during their exponential growth phase in unfiltered RAS WW at 17 ± 0.5 °C. Growth and nutrient uptake efficiency of six green and four non-green microalgae strains were compared between WW and reference growth medium in batch monocultures. The effect of three different LED grow light spectra on growth and nutrient uptake efficiency were compared for four green microalgae in WW. The specific growth rate (SGR, 0.5-0.8 d−1) and removal of NO3-N (N%, 57–96%) and PO4-P (P%, 78–94%) of green microalgae in WW in 4 days were comparable to the results obtained in the reference medium (SGR, 0.6-0.9 d−1, N%, 59–99%; P%, 86–99%). In contrast, non-green microalgae had negligible growth (SGR, from -0.1 to 0.2 d−1) and poor nutrient removal (N%, 1–29%; P%, 0–34%) in both growth media after 9 days. The three LED spectra did not differ on their effect on growth and nutrient removal of three green microalgae in WW after 4 days, while the fourth tested species, Haematoccocus pluvialis, had its highest nutrient removal after 8 days under a specific LED spectrum. Current results show that RAS WW supports well green microalgae growth in batch cultures in temperatures common in Nordic RAS and that continuous spectrum LED grow lights can induce high removal of dissolved nutrients. Our findings lend support to the concept of using temperate zone microalgae for nutrient removal and recycling from RAS WW.

Decoupling Abundance and Biomass of Phytoplankton Communities Under Different Environmental Controls: A New Multi-Metric Index

Increased nutrient delivery to estuarine systems results in elevated growth of primary producers. This is evidenced by high chlorophyll concentrations and increased frequency of phytoplankton blooms. However, shifts in nutrient loads to estuarine ecosystems can also cause modifications in the structure of phytoplankton communities which can have adverse impacts right through the food web. Acknowledging these modifications is imperative if response mechanisms are to be fully understood. In this study Ireland’s current Water Framework Directive (WFD) tool for determining the status of phytoplankton communities was built upon to encompass not only biomass and bloom frequency but also community structure (diversity and evenness) and abundance. This method allows for comparison with site- and date- specific environmental data which could give an indication of cause and effect relationships. The newly developed Phytoplankton Index performed well against current methods to determine ecological status. Furthermore, it had a better agreement with other physico-chemical and biological WFD parameters. Statistical analysis captured the relationship between the Phytoplankton Index and physico-chemical parameters allowing for a more detailed look at the impact of disturbance on the system. The inclusion of community structure acknowledged the imbalances in the phytoplankton communities of some systems even when frequent blooms are not evident. In bloom conditions, the disparity between the chlorophyll and abundance metrics within the Phytoplankton Index can be linked to winter dissolved inorganic nitrogen concentration and forms, temperature and light conditions. Development of the Phytoplankton Index will allow not only for compliance with WFD requirements, but is a method for understanding and assessing ecosystem health of estuarine phytoplankton communities over spatial and temporal timelines in line with changes in physiochemical parameters.

Explaining Recent Firm Growth in Dutch Horticulture

SummaryDutch horticultural firms have expanded rapidly in recent decades, both in terms of their production area as well as in number of employees. In particular in the production of fresh fruits and vegetables, a number of very large horticultural firms emerged with often more than 100 employees, operating on tens of hectares of greenhouses. A standard explanation for firm growth is that firms want to benefit from economies of scale, where the increased scale of production would ensure lower average (fixed) production costs. This article however shows that cost reduction due to economies of scale is not the main driver behind the growth in horticultural firm size. In fact, our empirical evaluation shows that larger horticultural firms face higher average production costs compared to smaller firms. However, these higher production costs are compensated by the on average higher and more stable output prices obtained by larger firms. This positive effect of firm size on firm revenues therefore provides a different rationale for the recent growth in average size of Dutch horticultural firms. As a result, our findings demonstrate that revenue‐related aspects are becoming more important in understanding firm growth of primary producers in the horticultural sector.

Interannual Variability of the Ice Cover and Primary Production of the Kara Sea

Model and satellite data have been used to analyze interannual changes in the primary production (PP), ice-free area, and water temperature in the Kara Sea between 2002 and 2016. Significant positive trends for changes in the surface water temperature were recorded for all regions of the Kara Sea (R2 = 0.37–0.61, p < 0.05). A reliable trend toward an increase in ice-free surface area was observed only for the Yenisei estuary. PP growth trends were significant in the northern Region of the sea and the Yenisei estuary. The decrease in ice cover area for the entire Kara Sea amounted to 1% per year. A PP minimum was recorded in 2003, and a maximum, in 2011. The average increase in Kara Sea PP during the study period was 2% per year. The most rapid increase in Kara Sea PP was observed during the spring season (April–May). The increase in annual PP was mostly due to the expansion of the ice-free area. The increase in the phytoplankton photosynthesis rate could not have resulted in the increase in PP, because the average photosynthesis rate for the entire sea decreased during the study period. Considerable region-specific variation in the patterns of interannual Changes in PP in the Kara Sea were observed.

Virus-mediated transfer of nitrogen from heterotrophic bacteria to phytoplankton

Abstract. Lytic infection of bacteria by viruses releases nutrients during cell lysis and stimulates the growth of primary producers, but the path by which these nutrients flow from lysates to primary producers has not been traced. This study examines the remineralisation of nitrogen (N) from Vibrio lysates by heterotrophic bacterioplankton and its transfer to primary producers. In laboratory trials, Vibrio sp. strain PWH3a was infected with a lytic virus (PWH3a-P1) and the resulting 36.0 µmol L−1 of dissolved organic N (DON) in the lysate was added to cultures containing cyanobacteria (Synechococcus sp. strain DC2) and a natural bacterial assemblage. Based on the increase in cyanobacteria, 74 % (26.5 µmol L−1 N) of the DON in the lysate was remineralised and taken up. Lysate from Vibrio sp. strain PWH3a labeled with 15NH4+ was also added to seawater containing natural microbial communities, and in four field experiments, stable isotope analysis indicated that the uptake of 15N was 0.09 to 0.70 µmol N µg−1 of chlorophyll a. The results from these experiments demonstrate that DON from lysate can be efficiently remineralised and transferred to phytoplankton, and they provide further evidence that the viral shunt is an important link in nitrogen recycling in aquatic systems.

Combined effect of copper sulfate and water temperature on key freshwater trophic levels – Approaching potential climatic change scenarios

This work relied on the use microcosms to evaluate the individual and the combined effects of different levels of copper sulfate (0.0, 0.013, 0.064 and 0.318mg Cu L−1) – a fungicide commonly exceeding allowable thresholds in agricultural areas – and a range of water temperature increase scenarios (15, 20 and 25°C) on freshwater species belonging to different functional groups. Hence, the growth inhibition of primary producers (the microalgae Raphidocelis subcapitata and the macrophyte Lemna minor), as well as the survival and feeding behavior of a shredder species (the Trichoptera Schizopelex sp.) were evaluated. The results revealed that copper was toxic to primary producers growth, as well as shredders growth and survival, being the growth of L. minor particularly affected. Higher water temperatures had generally enhanced the growth of primary producers under non-contaminated (microalgae and macrophytes) or low-contaminated (macrophytes) conditions. Despite the tendency for a more pronounced toxicity of copper under increasing water temperatures, a significant interaction between the two factors was only observed for microalgae. Since the test organisms represent relevant functional groups for sustaining freshwater systems functions, the present results may raise some concerns on the impacts caused by possible future climate change scenarios in aquatic habitats chronically exposed to the frequent or intensive use of the fungicide copper sulfate.