Primary Cultures Of Human Kidney Cells Research Articles

In vitro evidence from tissue cultures to prove existence of rabbit and human renotropic growth factor

When synchronized primary rabbit kidney monolayer cell cultures were incubated with a medium that contained uninephrectomized rabbit serum, the incorporation of tritiated thymidine into DNA was much greater than that with a medium that contained normal rabbit serum. The maximal growth-stimulating activity of uninephrectomized rabbit serum on thymidine incorporation into confluent monolayer cultures and sparsely cultured cells was increased approximately two- and fivefold, respectively, above that of normal serum. We conclude that uninephrectomized sera contain a growth-stimulating factor. Similarly, sera from a uninephrectomized human kidney donor and a cancer patient stimulated primary human kidney cell cultures to incorporate tritiated thymidine at levels far beyond those with their prenephrectomized sera. The maximal growth-stimulating activities of uninephrectomized human sera with confluent monolayer cultures and sparsely cultured cells were approximately six- and 13-fold, respectively, above those with the prenephrectomized sera. Thus, the maximal growth-stimulating activity in uninephrectomized human sera was much greater than that in uninephrectomized rabbit sera. Differences in the maximal growth-stimulating activities may be due to differences in the relative number of cell types, for example, epithelial versus fibroblast, present in these kidney cell cultures. By the use of primary cultured cells from other organs such as rabbit skin and human prostate and primary kidney cultures from three species (rabbit, human, and hamster), the growth-stimulating factor in uninephrectomized sera showed organ specificity, that is, renotropic and species specificity in this in vitro assay system.(ABSTRACT TRUNCATED AT 250 WORDS)

Examination of adult primary human kidney cell cultures for adventitious viral agents.

SummaryTwelve primary adult human kidney cell cultures were examined by several techniques for the presence of adventitious viral agents. Although no definite cytopathic effects were observed in 11 of the cell cultures, 7 of these exhibited suspicious fluorescent foci after staining by the indirect fluorescent-antibody technique 4 to 7 weeks after initiation of the cultures. Electron microscopy (EM) studies, utilizing negative staining and thin sections, were done on several of the cell cultures, but no virus particles were observed. Passage attempts in primary and continuous cell cultures did not reveal the presence of transmissible viral agents in any of the 11 cell cultures. One cell culture, obtained from a 58-year-old male who died shortly after a myocardial infarction, showed cytopathic changes 28 days after initiation. EM examination of the cell pellet showed typical adenovirus particles which were identified as a type 2 adenovirus. In contrast to observations with subhuman primate tissues, latent ...