Lactuca Serriola Research Articles

Species composition of weeds in soybean Glycine max crops in the Belgorod region

The weed species were studied based on monitoring data in soybean crops in the Belgorod region. A total of 37 species of weed plants were found, most of which belong to the families of Compositae, Gramineae, and Cruciferae. The significance of each species in soybean crops was evaluated on its partial activity, which was determined based on both its occurrence and projective cover. Chenopodium album, Echinochloa crusgalli, Amaranthus retroflexus, Fallopia convolvulus and two Setaria species (S. viridis and S. pumila) demonstrated the highest scores of partial activities. Cirsium incanum, Persicaria hydropiper, Convolvulus arvensis, Solanum nigrum, Polygonum aviculare s. str., Xanthium strumarium were shown to have rather high scores of partial activities. About a third of species (13) are low-active: Cyclachaena xanthiifolia, Sonchus arvensis, Euphorbia virgata, Descurainia sophia, Stachys annua, Artemisia vulgaris, Tripleurospermum inodorum, Lactuca serriola, Consolida regalis, Artemisia absinthium, Sonchus asper, Centaurea cyanus, S. italica. Twelve weed species were rated as inactive in soybean crops of the Belgorod region. The results of the study may be used to develop a regional strategy for protecting soybean crops from weeds in the Belgorod region.

QTL mapping and transcriptome analysis of seed germination under PEG-induced water stress in Lactuca spp.

The impact of limited water availability on lettuce growth has been well documented. However, the mechanisms by which lettuce controls seed germination under water stress remain unknown. Germination percentage was evaluated in the cv. Salinas (Lactuca sativa) (L. sativa) × US96UC23 (Lactuca serriola) (L. serriola) recombinant inbred line (RIL) population and USDA germplasm collection using 10% polyethylene glycol (PEG). About 50% of both populations displayed less than 90% germination. The average broad-sense heritability (H2) for germination percentage was 0.81 across both populations. Two quantitative trait loci (QTL) for germination percentage were identified on chromosomes 4 and 8 in the RIL population. The RNA-Seq and network analyses of wild lettuce, US96UC23, were performed using the control (distilled water, dH2O) and treatment (10% PEG) datasets. The number of differentially expressed genes (DEGs) was 4,095. The top 20 gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were assessed by enrichment analysis. The consensus network analysis captured 44 modules. Gene networks were constructed for the top 20 hub genes in 10 significant modules from each dataset. This study comprehensively explains QTL, GO terms, KEGG pathways, and gene networks associated with lettuce seed germination under osmotic stress.

Methyl jasmonate improves rubber production and quality in Lactuca Serriola.

The increase in demand for natural rubber has led to the search for alternative sources. Lactuca serriola is emerging as a promising candidate, as the quality of the natural rubber it produces is comparable to that of the Pará Rubber Plant, Hevea brasiliensis. This study examines the effect of methyl jasmonate (MeJA), a known elicitor, on the expression of key rubber biosynthesis pathway genes (HMGR1, HMGS1, CPT2, and SRPP1) in the latex of L. serriola plants. The expression levels of these genes increased significantly after the foliar application of 200 and 400 µM MeJA. The highest relative expression level for HMGR1, HMGS1, CPT2 and SRPP1 was 3.74, 18.56, 11.91and 16.59 fold respectively. Furthermore, the rubber content in L. serriola showed a significant rise post-treatment compared to the control with increasing the level of MeJA (6.19%, 7.24% and 7.85% which correspond to 0, 200 and 400 µM). Gel permeation chromatography revealed an augmentation in the molecular weight of extracted natural rubber from treated plants. Samples treated with 400 µM of MeJA had the highest molecular weight (1570 kg mol-1) compared to control (1186 kg mol-1). This study has demonstrated that MeJA, through the regulation of rubber biosynthesis genes, is capable of enhancing the quality and quantity of natural rubber extracted from alternative sources, such as L. serriola.

WEEDS AND WILD PLANTS AS NATURAL HOSTS OF CUCUMBER MOSAIC VIRUS: A CASE STUDY FROM WESTERN SLOVAKIA

Cucumber mosaic virus (Bromoviridae) is a worldwide widespread pathogen affecting more than 1200 plant species. Although more attention is paid to CMV research on agricultural crops, vegetables or ornamental plants, this work was focused on the analysis of several weeds and wild plants as potential natural hosts of CMV. For this purpose, samples from two different agroecological locations were tested by DAS-ELISA using CMV-specific antibodies, revealing nine CMV-positive plant species. CMV isolates from greater burdock (Arctium lappa L.), rough hawksbeard (Crepis biennis L.), wood avens (Geum urbanum L.), prickly lettuce (Lactuca serriola L.), black medick (Medicago lupulina L.), bitter dock (Rumex obtusifolius L.), field sowthistle (Sonchus arvensis L.), white clover (Trifolium repens) and white violet (Viola alba Besser.) were further characterized by sequencing the complete CP gene, disclosing their affiliation to the CMV Group II. We have shown that weeds and wild plant species can be effective hosts for CMV and can serve as virus reservoirs from which the virus can be spread to cultivated crops.

Methyl jasmonate effects on Lactuca serriola L.: Antioxidant defense and bioactive compound changes.

The effect of methyl jasmonate (MeJA) foliar spray on the activity of antioxidant enzymes-Superoxide dismutase (SOD), Catalase (CAT), Ascorbate peroxidase (APX), and Guaiacol peroxidase (GPX)-along with assessments of total phenolic and flavonoid contents and antioxidant activity (IC50), was examined in Prickly lettuce (Lactuca serriola L.). The study involved treating plants with three MeJA solutions (0, 200, and 400 µM) and harvesting samples at four distinct time intervals. Varied MeJA concentrations and time intervals resulted in a substantial increase in the activity of all the antioxidant enzymes investigated in this study. Both concentration levels and time courses exhibited progressive outcomes. Moreover, MeJA treatment led to elevated levels of total phenolic and flavonoid contents, reaching peaks of 17.02 (mg GAL/g DW) and 8.3 (mg QUE/g DW), respectively, particularly in response to the 400 µM concentration. However, the total flavonoid content did not show any significant variation between the two concentrations. Based on the half-maximal inhibitory concentration (IC50) values, the antioxidant activity in MeJA-treated plants was found to be lower compared to the controls. However, our findings suggest that, under specific conditions discussed in this study, MeJA has the potential to enhance the nutritional value of L. serriola.

Inter- and Intraspecific Competition in Invasive Lactuca serriola and Co-Occurring Weedy Plant Species.

While invasive success of alien plant species is often attributed to their superior competitive abilities, it is also suggested that competitive ability depends on the target species of competition and resource availability. In addition, it remains unclear whether invaders and co-occurring plants in the introduced area exhibit distinctive inter- and intraspecific competitive intensities. This study aimed to evaluate the competitive ability of a successful invader, Lactuca serriola, through a combination of field surveys and a growth chamber experiment. First, we assessed biodiversity and the biomass of co-occurring plants in both L. serriola-invaded and uninvaded plots across nine sites in South Korea. Subsequently, a pairwise competition experiment was conducted between L. serriola and three weedy plant species commonly found in the invaded plots, Chenopodium album, Erigeron canadensis, and Oenothera biennis, under differential nutrient levels. Diversity indices of plant communities and the biomass of most co-occurring plants showed no significant difference between invaded and uninvaded plots. L. serriola and testing weedy plants exhibited mutually negative effects on biomass when grown together in the same pot, with the intensity of interspecific competition being comparable across nutrient treatments. Notably, intraspecific competition of L. serriola was weaker than testing weedy plants, particularly manifest in the high-nutrient treatment. The results of both field and growth-chamber studies demonstrated that L. serriola was not a particularly strong competitor compared to its neighboring weedy plants. Its successful invasion can be partially attributed to its weak intraspecific competition intensity, which potentially facilitate successful establishment with high density.

Differential Sources of Resistance from Lactuca serriola Against Three Races of Xanthomonas hortorum pathovar vitians (Brown, 1918) Morinière et al. 2020 Causing Bacterial Leaf Spot of Lettuce.

Bacterial leaf spot (BLS) of lettuce (Lactuca sativa L.) is caused by the bacterium Xanthomonas hortorum pv. vitians which is hypothesized to have at least three races of the pathogen present in North America as defined by their differential resistance phenotypes in lettuce cultivars/accessions. Though resistance to X. hortorum pv. vitians race 1 has been identified in cultivated lettuce, numerous other X. hortorum pv. vitians strains cause disease on cultivars carrying this resistance locus. Thus far, resistance to these 'additional' X. hortorum pv. vitians strains has not been adequately described in L. sativa or in any other wild Lactuca species sexually compatible with cultivated lettuce. We have performed an extensive screening of approximately 500 Lactuca accessions from L. sativa, L. serriola, L. saligna, L. virosa, L. aculeata, L. altaica, and L. perennis species to identify accessions resistant to these additional X. hortorum pv. vitians races. Following the initial screenings, greenhouse tests confirmed that X. hortorum pv. vitians race 2 and race 3 could be defined using Lactuca sativa accessions. Race 2 strain BS3217 had an incompatible response (hypersensitive response) on ten Lactuca serriola accessions including PI491114 and PI491108, while race 1 (BS0347) and race 3 (BS2861) strains of X. hortorum pv. vitians showed a compatible response (disease) on these genotypes. L. serriola accession ARM09-161 (and selections derived from it) was the only genotype resistant to the race 3 strain BS2861. L. serriola accessions identified in this study to be resistant to race 2 and race 3 of X. hortorum pv. vitians, together with race 1 resistant cultivars, can be used for pyramiding resistance loci against the three races of the BLS-causing pathogen.

Relationship between Salmonella enterica attachment and leaf hydrophobicity, roughness, and epicuticular waxes: a focus on 30 baby-leaf salads.

The first step in the contamination of leafy vegetables by human pathogens is their attachment to the leaf surface. The success of this is influenced strongly by the physical and chemical characteristics of the surface itself (number and size of stomata, presence of trichomes and veins, epicuticular waxes, hydrophobicity, etc.). This study evaluated the attachment of Salmonella enterica to 30 baby-leaf salads and tested whether the differences found among them were related to the following leaf traits: hydrophobicity, roughness, and epicuticular waxes. Differences in susceptibility to contamination by S. enterica were found between the 30 baby-leaf salads investigated. The lowest attachment was found in wild lettuce (Lactuca serriola L.) and lamb's lettuce 'Trophy F1' (Valerianella locusta [L.] Laterr.), with values of 1.63 ± 0.39 Log(CFU/cm2) and 1.79 ± 0.54 Log(CFU/cm2), respectively. Attachment was correlated with hydrophobicity (measured as contact angle) (r = -0.39) and epicuticular waxes (r = -0.81) but not with roughness (r = 0.24). The most important wax components for attachment were alcohols and, in particular, the three-dimensional (3D) wax crystals of C26 alcohol, but fatty acids probably also had a role. Both these compounds increased hydrophobicity. The presence of thymol, whose antimicrobial properties are well known, was found in lamb's lettuce. The findings of this study can help to predict and control the attachment and contamination of leafy salads by enterobacteria. They also provide useful information for breeding programs aiming to develop cultivars that are less susceptible to human pathogens, enhancing the food safety of vegetables. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

DNA methylation variations underlie lettuce domestication and divergence

BackgroundLettuce (Lactuca sativa L.) is an economically important vegetable crop worldwide. Lettuce is believed to be domesticated from a single wild ancestor Lactuca serriola and subsequently diverged into two major morphologically distinct vegetable types: leafy lettuce and stem lettuce. However, the role of epigenetic variation in lettuce domestication and divergence remains largely unknown.ResultsTo understand the genetic and epigenetic basis underlying lettuce domestication and divergence, we generate single-base resolution DNA methylomes from 52 Lactuca accessions, including major lettuce cultivars and wild relatives. We find a significant increase of DNA methylation during lettuce domestication and uncover abundant epigenetic variations associated with lettuce domestication and divergence. Interestingly, DNA methylation variations specifically associated with leafy and stem lettuce are related to regulation and metabolic processes, respectively, while those associated with both types are enriched in stress responses. Moreover, we reveal that domestication-induced DNA methylation changes could influence expression levels of nearby and distal genes possibly through affecting chromatin accessibility and chromatin loop.ConclusionOur study provides population epigenomic insights into crop domestication and divergence and valuable resources for further domestication for diversity and epigenetic breeding to boost crop improvement.

Phytotoxicity of phenolic compounds of Pistacia vera leaves and its potential use as bioherbicide

In recent years, the intensive and uncontrolled use of synthetic herbicides has resulted in severe soil and water contamination, inducing herbicide resistance and damage to living organisms. The aim of this work was to study the potential bioherbicidal effect of extracts obtained from pistachio leaves. Aqueous extracts (3, 6, 12, and 25%) were prepared and their inhibitory effect on three weed species (Lolium rigidum, Solanum nigrum, and Lactuca serriola) and four crops (maize cv. Celestquatro, barley cv. Yurico, wheat cv. Rimbaud, and lentil cv. Castellana) was studied. Germination bioassays in Petri dishes and in vivo tests on organic and inert substrates were carried out with the extracts. In addition, the phenolic compounds present in the extracts were analysed and germination bioassays of the pure and individual compounds identified were performed. The results showed that the extracts caused a significant suppression of germination in weeds, being more remarkable for broadleaf species. For crops, no adverse effects on germination rates were observed. Inert substrate experiments showed a stronger inhibitory influence on weed germination, whereas organic substrate did not. Autumn leaf extracts showed a higher content of total phenols. Component analysis and bioassay showed that gallic acid, catechin, myricetin, and rutin were identified as individual phenolic compounds in the extracts and demonstrated some allelopathic activity. It may be concluded that pistachio leaf extracts had season-varied allelopathic activity, which is attributed to the combined bioactivity with those individual phenolic compounds.

Effect of Different Levels of Foliar Spray of Water Soluble Fertilizer NPK on Growth, Yield and Quality of Prickly Lettuce

A trial was conducted to evaluate the foliar application of fertilizer on growth, yield & quality of prickly lettuce at Vegetable Research farm, Department of Horticulture, SHUATS. The objectives of this study were to find out suitable combination of water- soluble fertilizer of nitrogen, potassium and phosphorus for growth, yield and quality of prickly lettuce and to estimate the economics of the various treatments. The experiment was laid out in randomized block design (R.B.D) with 3 replications and 8 treatments. Results revealed that treatment T4 performed best in terms of growth parameters viz; plant height, leaf area , number of leaves per plant, plant spread, leaf area , yield parameters viz; yield per plant, yield per hectare and quality parameters viz; TSS and ascorbic acid.

Chemical composition and antimicrobial activity of an apolar extract from Lactuca serriola L. leaves

Lactuca serriola L. is an annual herbaceous plant species belonging to the Asteraceae family, called Prickly lettuce, Wild lettuce, and Jagged lettuce. L. serriola grows on grassy and rocky slopes in many regions in Europe, Asia, Australia, Africa, and North, Central and South America. The plant is used as a natural pharmaceutical agent in primary health care due to its analgesic, anti-inflammatory, antibacterial, and antioxidant properties, which are attributed to the compounds it contains. However, this is the first study demonstrating the antimicrobial activity of L. serriola essential oil. In this study, L. serriola essential oil was obtained by hydrodistillation of plant leaves and analyzed by Gas Chromatography-Mass Spectrometry (GS-MS); subsequently, antimicrobial properties of the essential oil were evaluated using agar dilution and broth microdilution methods. As a result of the GS-MS analysis, hexadecanoic acid, oleic acid, myristic acid, hexahydrofarnesyl acetone, β-ionone, and n-tetradecyl butanoate were found as dominant components in the plant essential oil with the rate of 6.61%, 4.84%, 2.05, 1.55%, 1.3%, and 1.09% (v/v), respectively. The MIC value of L. serriola essential oil against Enterobacter aerogenes,Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Candida tropicalis was found to be 0.94 μLmL−1, and MIC values were determined as 1.87 μLmL−1 and 0.47 μLmL−1 against Klebsiella pneumonia and Candidaparapsilosis, respectively. These results have opened a new horizon regarding the usability of L. serriola essential oil as a pharmacological therapeutic antibacterial and antifungal agent.

A WOX homolog disrupted by a transposon led to the loss of spines and contributed to the domestication of lettuce.

The loss of spines is one of the most important domestication traits for lettuce (Lactuca sativa). However, the genetics and regulation of spine development in lettuce remain unclear. We examined the genetics of spines in lettuce using a segregating population derived from a cross between cultivated and wild lettuce (Lactuca serriola). A gene encoding WUSCHEL-related homeobox transcription factor, named as WOX-SPINE1 (WS1), was identified as the candidate gene controlling the spine development in lettuce, and its function on spines was verified. A CACTA transposon was found to be inserted into the first exon of the ws1 allele, knocking out its function and leading to the lack of spines in cultivated lettuce. All lettuce cultivars investigated have the nonfunctional ws1 gene, and a selection sweep was found at the WS1 locus, suggesting its important role in lettuce domestication. The expression levels of WS1 were associated with the density of spines among different accessions of wild lettuce. At least two independent loss-of-function mutations in the ws1 gene caused the loss of spines in wild lettuce. These findings provide new insights into the development of spines and facilitate the exploitation of wild genetic resources in future lettuce breeding programs.

KARYOSYSTEMATICS STUDY OF SOME ASTERACEAE SPECIES (COMPOSITAE) IN SAUDI ARABIA

Chromosome numbers were reported for 10 species of family Asteraceae from Saudi Arabia. The new count was reported in Pulicaria schimperi L. Karyotype analysis weremade for all species, Conyza bonariensis (L.) Cronquist 2n=18, Bidens pilosa L., 2n=70, Pluchea dioscoridis (L.) DC. 2n=32, Pulicaria schimperi L. 2n=12, Lactuca serriola L.2n=18, Launaea capitata (Spreng.) Dandy 2n=18, Launaea resedifolia (L.) Kuntze 2n=18, Launaea nudicaulis (L.) Hook. F. 2n=18, Reichardia tingitana (L.) Roth 2n=16, and Sonchus oleraceus L. 2n=32, belonging to 4 tribes: Astereae, Heliantheae, Inulea and Lactuceae. Pulicaria schimperi L. has the lowest chromosome number (2n=12), while the highest number of (2n=70) is recorded in Bidens pilosa L.

Biogenic synthesis of MoO3 nanoparticles using extract of Lactuca Serriola plant of Asteraceous flora: palynomorphological studies and environmental remediation applications

The development of environmental remedies using ecofriendly and green technologies is attracting increasing research attention. Herein, the green synthesis of molybdenum oxide (MoO3) nanoparticles (NPs) using extract of selected Asteraceous flora species is reported for the first time along with the investigation of the degradation ability for organic pollutants and pollen morphological characteristics of wetland species of the family Asteraceae. An X-ray diffraction analysis confirmed the formation and crystalline structure of the MoO3 NPs. Fourier transform infrared spectra of Lactuca Serriola extract indicated the presence of capping agents, reducing agents, and phytochemicals. Scanning electron microscopy images revealed the almost spherical shape of the MoO3 NPs. A qualitative and quantitative palynological investigation of 21 species belonging to 12 genera of the Asteraceae from different wetlands of Azad Jammu and Kashmir is also reported. The MoO3 NPs degraded 99% of crystal violet dye within 30 minutes with a degradation rate of 0.022 min−1. The cost-efficient and green synthesis method for MoO3 NPs with high catalytic activity provides a new platform for the development of photocatalysts.

New hosts and diagnostic characteristics of Orobanche crenata (Orobanchaceae) in Egypt

The holo-parasitic weed Orobanche crenata Forrsk. is a threat to economically important legumes and vegetables in Mediterranean countries, including Egypt. The crenate broomrape attacks several wild and cultivated plant species, and documentation of new hosts of the parasite is always required. To the best of our knowledge, this study is the first report of parasitism of the crenate broomrape on two ornamental species, Arctotis fastuosa Jacq. and Callistephus chinensis (L.) Nees. (Asteraceae). We also recorded for the first time its parasitism on the wild weeds (Ammi majus L., Lactuca serriola L., and Melilotus indicus (L.) All.) and the cultivated plant species (Carthamus tinctorius L. and Tropaeolum majus L.) from Egypt. The occurrence of O. crenata parasitism was confirmed by the attachment of its haustoria to the roots of host plants. The incidence of crenate broomrape disease was estimated for the seven species. The study also provides a morphological description of the polymorphic O. crenata on the samples from Egypt and determines the most useful characteristics for its easier identification in the field.

Point mutations including a novel Pro-197-Phe mutation confer cross resistance to acetolactate synthase (ALS) inhibiting herbicides in Lactuca serriola in Australia.

Control of prickly lettuce has become increasingly difficult for lentil growers in southern Australia due to widespread resistance to common herbicides, a lack of alternative herbicide options, and prolific production of highly mobile seed. This study aimed to quantify ALS-inhibiting herbicide resistance in the Mid North (MN) and Yorke Peninsula (YP) of South Australia, characterise the resistance mutations present, and investigate population structure and gene flow in this species. Resistance was identified in all populations tested, with average survival of 92% to chlorsulfuron and 95% to imazamox + imazapyr. Five different amino acid substitutions were identified at Proline-197 of the ALS gene. There was no significant difference in the LD50 between plants with these five different substitutions when treated with metsulfuron-methyl, however, imidazolinone resistance level was higher in plants with a phenylalanine substitution and lower in plants with a serine. Population structure based on 701 SNPs and 271 individuals provided evidence for both the independent evolution of the same mutation in different populations, as well as frequent short to medium distance dispersal accompanied by occasional long-distance dispersal events. The overall inbreeding coefficient (FIS ) was calculated at 0.5174, indicating an intermediate level of outcrossing despite the cross-pollination experiment showing only low outcrossing. In the structure analyses, most individuals from YP were assigned to a single cluster, whereas most individuals from MN were assigned 50% to each of two clusters, indicating some genetic differences between these two regions, but also evidence for dispersal between them. Use of imidazolinone herbicides has selected for mutations conferring higher levels of resistance, such as the Pro-197-Phe mutation, and resulted in further spread of resistance in this species. This article is protected by copyright. All rights reserved.

Effect of the Lactuca serriola L. extract on the cytogenetic behaviours of Crocus sativus L. roots and its allelopathic potential

Effect of the Lactuca serriola L. extract on the cytogenetic behaviours of Crocus sativus L. roots and its allelopathic potential

Efficacy and economics of light‐activated sensor‐controlled sprayer for site‐specific weed control

AbstractAn increasing cost of controlling herbicide‐resistant weeds necessitates the development of cost‐effective weed control strategies in the semi‐arid U.S. Great Plains. Two separate field studies were conducted in 2013 and 2014 at the Montana State University Southern Agricultural Research Center near Huntley, MT to (1) evaluate a light‐activated sensor‐controlled (LASC) sprayer compared to a broadcast sprayer for effectiveness and cost savings for weed control in postharvest wheat (Triticum aestivum) stubble, and (2) determine the efficacy of LASC sprayer operated at two different speeds for weed control in fallow fields. Results indicated that both LASC and broadcast sprayers performed similarly for the effectiveness of a majority of herbicides tested on kochia [Bassia scoparia (L.) A.J. Scott] and prickly lettuce (Lactuca serriola L.). Paraquat alone or in tank‐mixture with linuron, and saflufenacil + 2,4‐D provided 93%–100% control of kochia, 98%–100% control of prickly lettuce, and reduced the total weed biomass by 75%–80% at 28 days after treatment (DAT), regardless of the sprayer used. A simple comparative economic analysis revealed that LASC sprayer reduced the overall chemical use by 23%–55% and resulted in cost savings of $5.83–$30.83 ha−1 across herbicide treatments. Herbicide efficacy was reduced up to 20% for kochia and prickly lettuce control when applied with LASC sprayer at a speed of 16 versus 8 km h−1. Altogether, these results indicate that the LASC sprayer can provide cost‐effective, site‐specific weed control in no‐tillage chemical fallow or postharvest wheat stubble.

Weed infestation of switchgrass (Panicum virgatum L.) crops

Purpose. Determination of the species composition of weeds in switchgrass crops and their influence on the formation of biomass productivity. Methods. Field, laboratory, quantitative-weighing, calculation-comparative, mathematical-statistical. Results. The results of studies on weed infestation of switchgrass crops and methods of their control are highlighted. A mixed type of weediness was observed in the crops, consisting of 19 species from 8 families, with a predominance of annual dicotyledonous species (75.0-87.2%). While in the third (91.7-95.1%) and fifth (61.0-91.7%) years of vegetation, perennial dicot species such as Erigeron canadensis, Lactuc aserriola L., Artemisia annua L., thistles ones, annual dicotyledons were not present. When analyzing the quantitative composition of weeds in the first year of vegetation on the weedy control, high level of weed infestation was observed, which amounted to 176 pcs./m2, in the second and third years 103-105 pcs./m2, in the fourth – 74 pcs./m2 and in the fifth – 39 pcs./m2 . In the variants where weed control was used in the third and subsequent years a decrease in the number of weeds by 83.5-88.4% was noted compared to the control. The use of herbicides on crops ensured efficiency in the first year of vegetation at the level of 72.3-76.9%, while in the fifth year it was 95.3-96.2%. Weed control allowed increasing the biomass yield to 10.7-10.8 t/ha, while the weeded control yielded 8.8 t/ha. Conclusions. The use of herbicides (Dialen super 464 SL, Caliber 50) led to a decrease in the number of weed plants from 28.6% in the first year of vegetation to 88.3% in the fifth year, providing a 72.3-97.6% efficiency depending on the year of vegetation of the crop. Weed controll in the fifth year of crop vegetation made it possible to obtain 11.0 t/ha of dry biomass, 11.9 t/ha of solid fuel, 220 GJ/ha energy from fuel.