Prey Habitat Research Articles

Friends because of foes: synchronous movement within predator-prey domains.

For prey, movement synchrony represents a potent antipredator strategy. Prey, however, must balance the costs and benefits of using conspecifics to mediate risk. Thus, the emergent patterns of risk-driven sociality depend on variation in space and in the predators and prey themselves. We applied the concept of predator-prey habitat domain, the space in which animals acquire food resources, to test the conditions under which individuals synchronize their movements relative to predator and prey habitat domains. We tested the response of movement synchrony of prey to predator-prey domains in two populations of ungulates that vary in their gregariousness and predator community: (i) elk, which are preyed on by wolves; and (ii) caribou, which are preyed on by coyotes and black bears. Prey in both communities responded to cursorial predators by increasing synchrony during seasons of greater predation pressure. Elk moved more synchronously in the wolf habitat domain during winter and caribou moved more synchronously in the coyote habitat domains during spring. In the winter, caribou increased movement synchrony when coyote and caribou domains overlapped. By integrating habitat domains with movement ecology, we provide a compelling argument for social behaviours and collective movement as an antipredator response. This article is part of the theme issue 'The spatial-social interface: A theoretical and empirical integration'.

Mesopredators have differing influences on prey habitat use and diel activity in a multipredator landscape

AbstractResource distribution, habitat structure, and predators greatly influence spatial and temporal landscape use by prey species. The “risky places” hypothesis establishes prey will proactively respond to predators' presence based on habitat cues, whereas the “risky times” hypothesis predicts prey will reactively respond by increasing vigilance in the presence of predators regardless of habitat cues. We fit a multiscale, Bayesian species interaction occupancy model with detection/non‐detection data to evaluate black‐tailed jackrabbit (Lepus californicus) and eastern cottontail rabbit (Sylvilagus floridanus) habitat use in the presence and absence of coyotes (Canis latrans), American badgers (Taxidea taxus), and swift foxes (Vulpes velox). We also evaluated how species‐specific predator presence modified temporal activity patterns of prey. Jackrabbits decreased habitat use in areas with greater forage and opted to use areas with greater visibility when coyotes or swift foxes were present. However, cottontails used habitat in open areas with greater visibility when American badgers were present and all other predators absent, suggesting dissimilar habitat‐use patterns dictated by predator‐specific risks. Both lagomorph species are nocturnal with segregated peaks of activity compared with predators, suggesting fine‐scale temporal use partitioning. Our results provide insights into predator–prey dynamics across heterogenous landscapes in a multi‐predator system.

Proactive cursorial and ambush predation risk avoidance in four African herbivore species.

Most herbivores must balance demands to meet nutritional requirements, maintain stable thermoregulation and avoid predation. Species-specific predator and prey characteristics determine the ability of prey to avoid predation and the ability of predators to maximize hunting success. Using GPS collar data from African wild dogs, lions, impala, tsessebes, wildebeest and zebra in the Okavango Delta, Botswana, we studied proactive predation risk avoidance by herbivores. We considered predator activity level in relation to prey movement, predator and prey habitat selection, and preferential use of areas by prey. We compared herbivore behaviour to lion and wild dog activity patterns and determined the effect of seasonal resource availability and prey body mass on anti-predator behaviour. Herbivore movement patterns were more strongly correlated with lion than wild dog activity. Habitat selection by predators was not activity level dependent and, while prey and predators differed to some extent in their habitat selection, there were also overlaps, probably caused by predators seeking habitats with high prey abundance. Areas favoured by lions were used by herbivores more when lions were less active, whereas wild dog activity level was not correlated with prey use. Prey body mass was not a strong predictor of the strength of proactive predation avoidance behaviour. Herbivores showed stronger anti-predator behaviours during the rainy season when resources were abundant. Reducing movement when top predators are most active and avoiding areas with a high likelihood of predator use during the same periods appear to be common strategies to minimize predation risk. Such valuable insights into predator-prey dynamics are only possible when using similar data from multiple sympatric species of predator and prey, an approach that should become more prevalent given the ongoing integration of technological methods into ecological studies.

Predator-prey interactions across hunting mode, spatial domain size, and habitat complexities.

Predator-prey interactions are a fundamental part of community ecology, yet the relative importance of consumptive and nonconsumptive effects (NCEs) (defined as a risk-induced response that alters prey fitness) has not been resolved. Theory suggests that the emergence and subsequent predominance of consumptive or NCEs depend on the given habitat's complexity as well as predator hunting mode and spatial domain sizes of both predator and prey, but their relative influence on the outcome of predator-prey interactions is unknown. We built agent-based models in NetLogo to simulate predator-prey interactions for three hunting modes-sit-and-wait, sit-and-pursue, and active-while concurrently simulating large versus small spatial domain sizes for both predators and prey. We studied (1) how hunting mode and spatial domain size interact to influence the emergence of consumptive or NCEs and (2) how, when NCEs do dominate, hunting mode and spatial domain separately or additively determine prey shifts in time, space, and habitat use. Our results indicate consumptive effects only dominate for active predators when prey habitat domains overlap completely with the predator's spatial domain and when sit-and-wait and sit-and-pursue predators and their prey both have large spatial domains. Prey are most likely to survive when they shift their time but most frequently shift their habitat. Our paper helps to better understand the underlying mechanisms that drive consumptive or NCEs to be most dominant.

Spatial prey availability and pulsed reproductive tactics: Encounter risk in a canid-ungulate system.

Predation risk is a function of spatiotemporal overlap between predator and prey, as well as behavioural responses during encounters. Dynamic factors (e.g. group size, prey availability and animal movement or state) affect risk, but rarely are integrated in risk assessments. Our work targets a system where predation risk is fundamentally linked to temporal patterns in prey abundance and behaviour. For neonatal ungulate prey, risk is defined within a short temporal window during which the pulse in parturition, increasing movement capacity with age and antipredation tactics have the potential to mediate risk. In our coyote-mule deer (Canis latrans-Odocoileus hemionus) system, leveraging GPS data collected from both predator and prey, we tested expectations of shared enemy and reproductive risk hypotheses. We asked two questions regarding risk: (A) How does primary and alternative prey habitat, predator and prey activity, and reproductive tactics (e.g. birth synchrony and maternal defence) influence the vulnerability of a neonate encountering a predator? (B) How do the same factors affect behaviour by predators relative to the time before and after an encounter? Despite increased selection for mule deer and intensified search behaviour by coyotes during the peak in mule deer parturition, mule deer were afforded protection from predation via predator swamping, experiencing reduced per-capita encounter risk when most neonates were born. Mule deer occupying rabbit habitat (Sylvilagus spp.; coyote's primary prey) experienced the greatest risk of encounter but the availability of rabbit habitat did not affect predator behaviour during encounters. Encounter risk increased in areas with greater availability of mule deer habitat: coyotes shifted their behaviour relative to deer habitat, and the pulse in mule deer parturition and movement of neonatal deer during encounters elicited increased speed and tortuosity by coyotes. In addition to the spatial distribution of prey, temporal patterns in prey availability and animal behavioural state were fundamental in defining risk. Our work reveals the nuanced consequences of pulsed availability on predation risk for alternative prey, whereby responses by predators to sudden resource availability, the lasting effects of diversionary prey and inherent antipredation tactics ultimately dictate risk.

Trait‐based analyses reveal global patterns in diverse diets of albacore tuna (Thunnus alalunga)

AbstractSimplifying complex species interactions can facilitate tracking and predicting functional responses to ecological stressors. This is important for highly migratory pelagic predators, exploiting diverse prey fields as they respond to dynamic environments. We reconstructed the historical resource use of albacore tuna (Thunnus alalunga) globally from the 1900s to 2015 and confirmed highly biodiverse diets with 308 prey species, and an additional 238 taxa at coarser taxonomic resolution. We synthesised prey diversity into seven functional trait guilds using hierarchical divisive clustering algorithms as a function of four traits that describe habitat use and influence predator–prey encounter rates – prey habitat association vertically in the water column, horizontally along the coastal to pelagic gradient, seasonal and diel vertical migratory behaviour. We explored variability in historical composition of albacore diets across geographies based on species identity, individual trait information and functional trait guilds using a multi‐matrix modelling framework. Taxonomic information remains important for trophic ecology, however, species‐based diet composition in albacore tuna was highly variable across geographies and years sampled, making interpretation of these patterns difficult. By simplifying species identity into habitat‐based traits, we highlight changes in prey resources consumed, such as the historical importance of near‐surface epipelagic prey resources from coastal to oceanic habitats, and seasonally migrating continental shelf prey, with less frequent pulses of deeper water and demersal taxa. Trait information and trait guilds serve as useful classification frameworks for identifying functionally redundant food web linkages across biodiverse prey, and will prove useful in tracking predators' foraging responses to changing resource availability.

Aerospace-foraging bats eat seasonably across varying habitats

Recent research has confirmed the efficiency of insectivorous bats as pest suppressors, underlining the ecological services they offer in agroecosystems. Therefore, some efforts try to enhance bat foraging in agricultural landscapes by acting upon environmental factors favouring them. In this study, we monitored a Miniopterus schreibersii colony, in the southern Iberian Peninsula. We intensively sampled their faeces and analysed them by metabarcoding to describe how the bent-winged bat diet would change with time, and to test whether their most-consumed prey would seasonally depend on different landscapes or habitats. Our results confirm that M. schreibersii are selective opportunist predators of moths, dipterans, mayflies, and other fluttering insects, shifting their diet to temporary peaks of prey availability in their foraging range, including both pest and non-pest insects. Supporting our hypothesis, throughout the year, M. schreibersii consume insects linked to diverse open habitats, including wetlands, grassland, diverse croplands, and woodland. The importance of each prey habitat varies seasonally, depending on their insect phenology, making bats indirectly dependent on a diverse landscape as their primary prey source. Bats' predation upon pest insects is quantitatively high, consuming around 1610 kg in 5 months, of which 1467 kg correspond to ten species. So, their suppression effect may be relevant, mainly in patchy heterogeneous landscapes, where bats' foraging may concentrate in successive outbursts of pests, affecting different crops or woodlands. Our results stress that to take advantage of the ecosystem services of bats or other generalist insectivores, keeping the environmental conditions they require to thrive, particularly a heterogeneous landscape within the colony's foraging area, is crucial.

Environmental favorability models as a tool to support trophic rewilding initiatives

Trophic rewilding is considered a conservation measure that aims to restore the trophic interactions. In north‐central Portugal, the Iberian wolf (Canis lupus signatus) is endangered mainly due to human persecution, triggered by wolf depredation on livestock. Several initiatives have occurred in this area to increase wolf wild prey availability by reintroducing/reinforcing roe deer (Capreolus capreolus) populations. However, the success of trophic rewilding initiatives relies on up‐to‐date scientific evidence, considering both ecological and social settings. Favorability models can be used to infer the most suitable habitats for roe deer settlement and expansion by analyzing the environmental variables known to influence species ecology and behavior. In this study, we infer how the prey habitat favorability can contribute to plan prey populations reinforcement processes as a tool for trophic chain restoration. Using 330 camera traps, we monitored the roe deer population over 8,940 km2 in north‐central Portugal. Field data were used to evaluate the environmental determinants of roe deer presence. By using the favorability function, we created a spatially explicit gradient of habitat favorability that allowed us to define key areas for the application of management measures. The selected sites are strategically positioned to promote the natural expansion of established roe deer populations and overlap the current distribution of Iberian wolf. These results are an important step toward the restoration of the trophic chain and to reduce human–wolf conflicts. Our proposal paves the way for future rewilding initiatives, especially where prey species are central to the survival of top predators.

Prey Seeking Behavior of Insect and Mite Predators

The foundation of integrated pest management (IPM) practices is biological control, and natural enemies are a more and more appealing option. A common class of invertebrate natural enemies utilized as bio-control agents is the insect and mite predators. In recent years, traditional methods of biological control have become less important than the preservation and management of naturally occurring predators in agricultural eco-systems. However, these strategies must be supported by a fundamental understanding of the ecology and prey selection behavior of the predatory species being used. Prey Habitat Selection, Prey Finding, Prey Acceptance, and Prey Suitability—these four components of prey seeking behavior—have been covered in this study. To find and identify their prey, predators respond to both semiochemical and physical inputs. Prey sex pheromones, which work as kairomones, or Herbivore Induced Plant Volatiles (HIPV), which operate as allomones or synomones, are responsible for these reactions. Other aspects of prey seeking behaviour, such as plant architecture, patch quality, the chemical makeup of the prey cuticle, etc. are also discussed. All together affect the foraging efficiency of natural enemies.

Predicting of Komodo dragon's potential prey habitat suitability using MaxEnt in Riung Nature Reserve, Flores, East Nusa Tenggara, Indonesia

Abstract. Pramatana F, Aini Y, Rammang N, Mau YS, Arsa IGBA, Mahmud A. 2023. Predicting of Komodo dragon's potential prey habitat suitability using MaxEnt in Riung Nature Reserve, Flores East Nusa Tenggara. Biodiversitas 24: 3128-3139. The Komodo dragon (Varanus komodoensis Ouwens, 1912) is a big lizard species from the Varanidae family that belongs to the Endangered category (EN) listed on the IUCN red list and Appendix I CITES. This study aimed to reveal the distribution of potential preys Komodo dragons in Rinca Island, Komodo National Park, Manggarai District, East Nusa Tenggara, Indonesia, using Maximum Entropy (MaxEnt), which was collected using rapid assessment methods. The presences of the Komodo dragon's potential prey come from direct and indirect observation or previous studies. We collected 510 points of Komodo dragon prey presence in Riung, Ngada District, East Nusa Tenggara, Indonesia from six species, including cattle, but only used 127 points for analysis based on the correlation. Long-tailed macaque, wild boar, civet, Timor deer, feral horses, and cows were the potential prey for komodo in Riung. Most of the points come from cattle, such as cows. On the other hand, we used environmental habitat to represent prey habitats such as elevation, slope, land surface temperature, moisture index, vegetation index, and distance from specific objects such as distance from agriculture, rivers, road, savanna, and settlement. Komodo dragon's potential prey in Riung was distributed in savanna, mangrove, and lowland forest. The result showed three suitable habitats for the Komodo dragon's potential prey dominated by low and moderate-suitability areas.

Mesopredator predation risk limits northern bobwhite nesting habitat

AbstractPredators can significantly limit the amount of available habitat for vulnerable prey. Encouraging spatial segregation of predators and prey through habitat management prescriptions may be a useful tool to increase productivity and abundances of prey species of conservation concern. We estimated the total area of low‐risk nesting habitat by interacting predator habitat selection models with northern bobwhite (Colinus virginianus) nest‐site selection models. We used trail cameras, transect sampling, spotlighting transects, and incidental tracking to survey and map occurrences of mesopredators at a site in western Oklahoma, USA. We created broad‐scale resource selection function models for bobwhite nest‐site and mesopredator habitat selection using generalized linear models to create maps of occurrence probability in ArcGIS. Bobwhite nests were more likely to be depredated in areas of high selection by coyotes (Canis latrans) and striped skunks (Mephitis mephitis) compared to areas with low mesopredator selection. Approximately 392 ha of bobwhite nesting habitat (17%) had low probabilities of selection by both coyotes and striped skunks. Our results demonstrate the need for incorporating species' interactions into estimations of available habitat. Based on the total area of highly selected cover by northern bobwhite, approximately 29% of the study area would have been deemed suitable for nesting. However, only 5% of that area is predicted to have a low predation risk.

Perception and kairomonal response of the coccinellid predator (Harmonia axyridis) to the fall armyworm (Spodoptera frugiperda) sex pheromone.

Pheromone cues released from hosts or prey are of crucial importance to natural enemies for prey and habitat location. The use of herbivorous insect sex pheromones has long been considered as a potential pest control alternative that is non-toxic and harmless to beneficials. We hypothesized that Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), a major predatory coccinellid beetle of the devastating migratory pest Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), could perceive and use the sex pheromone of S. frugiperda to locate its habitat. Here we tested the electrophysiological and behavioral responses of H. axyridis to the two components Z7-12:Ac and Z9-14:Ac of S. frugiperda sex pheromone by using electroantennography (EAG) and Y-tube bioassay. The 3D modeling of H. axyridis odorant-binding proteins (HaxyOBPs) and molecular docking were also performed. The results showed that both female and male H. axyridis exhibited significantly higher electrophysiological and behavioral responses to Z9-14:Ac at the concentrations of 0.001, 0.01, and 0.1μg/μL, while no significant electrophysiological and behavioral responses of H. axyridis were observed to Z7-12:Ac. The blend of Z7-12:Ac and Z9-14:Ac at the ratio of 1:100 had a significant attraction to both male and female H. axyridis at the concentrations of 0.01 and 0.1μg/μL based on electrophysiological and behavioral assays, but no significant behavioral responses were observed at the ratios of 1:9. According to the 3D modeling of HaxyOBPs and molecular docking, HaxyOBP12 has a good affinity with Z9-14:Ac. Z9-14:Ac is bound to the HaxyOBP12 by hydrogen bonding and hydrophobic interactions. However, there were no credible docking results between HaxyOBPs and Z7-12:Ac. Our findings revealed that H. axyridis can perceive Z9-14:Ac and could use it as a chemical cue to locate prey habitat. We speculated that Z7-12:Ac, which showed some antagonistic effect toward the response of H. axyridis to Z9-14:Ac, could improve the adaptability of S. frugiperda in the presence of predators. This study provides new insights into the application of pheromones to manipulate natural enemy behavior for pest control.

Spatiotemporal predictions of the alternative prey hypothesis: Predator habitat use during decreasing prey abundance

AbstractThe alternative prey hypothesis supposes that predators supported by a primary prey species will shift to consume alternative prey during a decrease in primary prey abundance. The hypothesis implies that during declines of one prey species, a predator modifies their behavior to exploit a secondary, or alternative, species. Despite occurring in many systems, the behavioral mechanisms (e.g., habitat selection) allowing predators to shift toward alternative prey during declines in the abundance of their primary prey are poorly understood. We evaluated habitat selection and use by a generalist predator with respect to two prey species during a dramatic decrease in the abundance of primary prey. Further, we evaluated how spatial variation in access to primary prey affected habitat selection, and assessed similarity and overlap between habitats used by each prey species. Coyotes (Canis latrans) exhibited decreasing selection for cottontail rabbits (Sylvilagus spp.; primary prey) during population decreases but did not shift habitat selection toward neonate mule deer (Odocoileus hemionus; alternative prey). Use of rabbit habitat remained high even during historically low rabbit abundance, while mule deer habitat was used in proportion to its availability. Coyotes seemingly do not make large shifts in habitat selection toward alternative prey following spatial and temporal decreases in the abundance of primary prey, but instead, take advantage of habitat overlap to facilitate prey‐switching behavior. Our work extends previous research conducted under the alternative prey hypothesis by explicitly evaluating the influence of habitat overlap between prey species and variation in access to prey habitat as factors affecting prey‐switching behaviors in predators.

Ecology of large felids and their prey in small reserves of the Yucatán Peninsula of Mexico.

Jaguars and pumas are top-predator species in the Neotropics that are threatened by habitat destruction, illegal poaching of their body parts and their favored prey, and by the human-wildlife conflicts that arise when predators attack livestock. Much of the remaining felid habitat in the Americas is in protected nature reserves that are too small and isolated to support local populations. Surrounding forests therefore play a vital role in felid conservation. Successful long-term conservation of these two felids requires evidence-based knowledge of their biological and ecological requirements. We studied population distributions of jaguars and pumas and their prey in and between two small, private reserves of the Northern Yucatán Peninsula, Mexico, with areas of 25 and 43 km2. During 2 years of camera trapping (2015 and 2016), we detected 21 jaguars, from which we estimated an average space requirement of 28-45 km2/individual. Dietary niche overlap exceeded random expectation. The most frequently occurring prey items in jaguar and puma diets were collared peccary and deer. Jaguar also favored nine-banded armadillos and white-nosed coati, while puma favored canids. Both felids avoided ocellated turkey. Overall, diet of jaguars was less species-rich, but similar in niche breadth, to that of pumas. A fluid use of space by both species, in 2015 tending toward mutual attraction and in 2016 toward partial exclusion of pumas by jaguars, combined with the high dietary overlap, is consistent with a dominance hierarchy facilitating coexistence. Jaguars and pumas favor the same prey as the people in local communities who hunt, which likely will intensify human-wildlife impacts when prey become scarce. We conclude that even small reserves play an important role in increasing the continuity of habitat for prey and large felids, whose generalist habits suppress interspecific competition for increasingly limiting prey that are largely shared between them and humans.

Dispersal and distribution of a generalist predator in habitats with multiple food resources

The ability to locate suitable food resources affects fitness in animals. Therefore, movements are necessary to optimize foraging in habitats where food is distributed in patches of different qualities. The aim of this work was to investigate the dispersal and distribution of females and males of the omnivorous mirid D. hesperus in mesocosms composed by food patches of different values in terms of fitness. In agreement with the Marginal Value Theorem (MVT) and the Ideal Free Distribution (IFD), individuals were expected to aggregate in the highest quality patches. Besides, the proportion of individuals in patches was predicted to be proportional to fitness, and interference among individuals was expected to rise as the density of individuals increased. Emigration rates were predicted to be higher for low- than for high-quality patches, while the opposite was predicted for immigration. Three types of habitats each with different combinations of food resources were tested: (1) habitat including patches of tomato plants with no-prey, and patches infested with either mite or whitefly; (2) with no-prey and whitefly; (3) with no-prey and mites. Each type of habitat was set up in a tomato greenhouse compartment and replicated four times. Individuals were tracked by mark-recapture methods using luminous paintings. The number of females and males in whitefly patches was significantly higher than in mite and no-prey patches, but a significant interaction sex*habitat and sex*patch was found. In habitats with only one type of prey, D. hesperus adults fitted the IFD, while in mixed prey habitats their distribution diverged from IFD. Interference was found to be significant, with female fitness decreasing as their density increased. Emigration rates were significantly lower for whitefly patches with a significant interaction patch*sex; the opposite was found for immigration. This research shows that it is unlikely that D. hesperus forage according to the omniscient principle of IFD and MVT; in contrast, it strongly suggests that it uses some simple rules to make decisions about inter-patch movement, and emigration from habitats and patches.

Wolf spatial behavior promotes encounters and kills of abundant prey.

Predators use different spatial tactics to track the prey on the landscape. Three hypotheses describe spatial tactics: prey abundance for prey that are aggregated in space; prey habitat for uniformly distributed prey; and prey catchability for prey that are difficult to catch and kill. The gray wolf (Canis lupus) is a generalist predator that likely employs more than one spatial hunting tactic to match their diverse prey with distinct distributions and behavior that are available. We conducted a study on 17 GPS collared wolves in 6 packs in Riding Mountain National Park, Manitoba, Canada where wolves prey on moose (Alces alces) and elk (Cervus canadensis). We evaluated wolf selection for prey density, habitat selection and catchability on the landscape through within-territory habitat selection analysis. We reveal support for both the prey habitat and prey catchability hypotheses. For moose, their primary prey, wolves employed a mixed habitat and catchability tactic. Wolves used spaces described by the intersection of moose habitat and moose catchability. Wolves selected for the catchability of elk, their secondary prey, but not elk habitat. Counter to our predictions, wolves avoided areas of moose and elk density, likely highlighting the ongoing space race between predator and prey. We illustrate that of the three hypotheses the primary driver was prey catchability, where the interplay of both prey habitat with catchability culminate in predator spatial behaviour in a multiprey system.

Large Felid and Peccary Habitat Use in Isolated and Contiguous Forest in Panamá: Implications for Conservation

Background and Research Aims Wildlife research in Panamá has focused primarily on protected areas along the Central Cordillera, where much of the remaining mature rainforest habitat is located. Information on large felid and prey habitat use in isolated habitats in Panamá is therefore limited. Here, we estimated occupancy and detection probabilities, as affected by habitat and anthropogenic influences, for 2 felid species (jaguars [ Panthera onca] and pumas [ Puma concolor]), and 2 prey species (white-lipped peccaries [ Tayassu pecari] and collared peccaries [ Pecari tajacu]). Methods Camera trap surveys were conducted during 2014–2015 at Cerro Hoya National Park (CHNP), an isolated remnant of tropical rainforest habitat, and Darién National Park (DNP), a large tract of continuous rainforest habitat. We used single-season, single-species occupancy modeling to estimate probabilities of detection and habitat use of our focal species. Results Three of the 4 focal species were detected at both sites, excluding white-lipped peccary at CHNP. Detection of jaguars and white-lipped peccaries at DNP was highest in February, while detection of collared peccaries at DNP and pumas at CHNP was highest in May and April, respectively. Peccary habitat use was uniform across sites and unaffected by habitat covariates. Both felids preferred habitat further away from anthropogenic disturbance, and jaguars preferred habitat at higher elevations than pumas. Conclusion We further confirm the presence of jaguars and likely local extirpation of white-lipped peccaries in CHNP. Temporal variations influenced detections of focal species. Habitat use of felids was negatively affected by anthropogenic disturbance and elevation. Implications for Conservation Habitat fragmentation and human activities negatively influenced habitat use of felids at both study areas. Given that CHNP serves as one of the last remnants of forest habitat outside the Central Cordillera, we recommend that CHNP be considered a top priority area for wildlife conservation in Panamá.

Moult chronology and strategies of sympatric Great (Ardenna gravis) and Sooty (A. grisea) Shearwaters based on stable isotope analysis

Moult is an energetically demanding period, during which flight may be impaired and foraging ranges may become constrained. During the non‐breeding period, GreatArdenna gravisand SootyArdenna griseaShearwaters migrate from South Atlantic breeding colonies to aggregate at North Atlantic feeding grounds. We investigated whether both shearwater species used coastal Newfoundland, Canada, as a moulting area and used stable isotope ratios (δ15N, δ13C) of recently moulted primary feathers (P1, P5, P10) to infer moult location/diet for both species. Moult scores indicated that both species finished their moult (i.e. P6–10) in coastal Newfoundland, which was further corroborated with similar stable isotope ratios for Great (δ15N = 15.17 ± 1.13‰; δ13C = −18.66 ± 0.54‰) and Sooty Shearwaters (15.54 ± 0.74‰; −18.43 ± 0.78‰); however, Sooty Shearwater moult was more advanced relative to that of Great Shearwater. In contrast, isotopic ratios of P1 and P5, which were grown before arriving in coastal Newfoundland, differed between and within species, suggesting divergent locations/diet during early moult. For Great Shearwaters, P1/P5 isotopic ratios were more variable (broader niche breadth) than P10, suggesting that some individuals started moulting in the South Atlantic prior to trans‐equatorial migration, whereas others start moulting in the North Atlantic Ocean. Sooty Shearwaters had two distinct groupings of either higher or lower δ15N in P1/P5, suggesting that individuals began moulting either on the Newfoundland Shelf or further offshore based on comparisons to reference shearwater feathers grown in known locations. These findings illustrate distinct locations and/or diets at the start of primary feather moult, both within and between species, but diets converged when aggregated together at the end of moult in coastal North America, where growing feathers of both species were sampled. More importantly, we identified an important area for both Sooty and Great Shearwaters to complete their moult in coastal Newfoundland. Protecting this moulting area would minimize disturbance and the impacts of threats (e.g. by‐catch) to both species during this energetically demanding period. The area has been suggested previously to be an important candidate area for protection due to annually persistent prey aggregations that can be spatiotemporally delimited based on specific prey habitat requirements.

Occurrence and Livestock Depredation Patterns by Wolves in Highly Cultivated Landscapes

Attacks by large predators on livestock are an important driver of conflicts. Consequently, knowledge about where predators occur, where livestock depredation takes place and what factors influence it will aid the mitigation of stakeholder conflicts. Following legal protection, wolves (Canis lupus) in Central Europe are recently spreading to areas dominated by agriculture, bringing them in closer contact with livestock. Here, we analyzed habitat selection and livestock depredation rates of 43 wolves identified by genotyping on the Jutland peninsula, consisting of mainland Denmark and the northernmost German federal state Schleswig-Holstein. Occupancy by resident wolves correlated positively with forest and other non-forested semi-natural land cover (habitat for natural ungulate prey), whereas occupancy by non-resident wolves correlated with increasing forest cover and sheep density. The latter effect likely reflected increased sampling probability of highly mobile dispersers killing livestock. We recorded 565 livestock depredation events (85 in Denmark and 480 in Schleswig-Holstein), of which 42% (55 in DK and 185 in SH) could be assigned to 27 individual wolves based on DNA evidence. Livestock (mostly sheep) were killed by wolves in 16% of the study area. Our results indicate that wolves mostly killed livestock as a context-dependent response, i.e., being dispersers in agricultural areas with low availability of wild ungulate prey and high livestock densities, and not because of behavioral preferences for sheep. Moreover, the livestock depredation was lower in areas with livestock protection measures (implemented in areas with established pairs/packs). We conclude that while wolf attacks on livestock in established wolf territories generally can be reduced through improvement of fences, livestock depredation by non-resident wolves in agricultural areas constitutes a bigger challenge. Albeit technically possible, the economic costs of implementing predator-proof fences and other preventive measures in such pastoral areas infrequently visited by wolves will be considerable. Experiences so far further indicate that lethal removal of identified “problem wolves” may be inefficient in practice.

Prey Foraging Behavior After Predator Introduction Is Driven by Resource Knowledge and Exploratory Tendency

Predator reintroductions are often used as a means of restoring the ecosystem services that these species can provide. The ecosystem consequences of predator reintroduction depend on how prey species respond. Yet, to date, we lack a general framework for predicting these responses. To address this knowledge gap, we modeled the impacts of predator reintroduction on foragers as a function of predator characteristics (habitat domain; i.e., area threatened) and prey characteristics (knowledge of alternative habitat and exploratory tendency). Foraging prey had the capacity to both remember and return to good habitat and to remember and avoid predators. In general, we found that forager search time increased and consumption decreased after predator introduction. However, predator habitat domain played a key role in determining how much prey habitat use changed following reintroduction, and the forager's knowledge of alternative habitats and exploratory inclinations affected what types of habitat shifts occurred. Namely, habitat shifts and consumption sacrifices by prey were extreme in some cases, particularly when they were pushed far from their starting locations by broad-domain predators, whereas informed foragers spent less time searching and displayed smaller reductions to consumption than their naïve counterparts following predator exposure. More exploratory foragers exhibited larger habitat shifts, thereby sacrificing consumption but reducing encounters by relocating to refugia, whereas less exploratory foragers managed risk in place and consequently suffered increased encounters while consuming more resources. By implication, reintroductions of predators with broad habitat domains are especially likely to impose foraging and movements costs on prey, but forager spatial memory state can mitigate these effects, as informed foragers can better access alternate habitat and avoid predators with smaller reductions in consumption.