Prevention Of Gastritis Research Articles

GAMBARAN PENGETAHUAN KELUARGA TENTANG GASTRITIS DI RUMAH SAKIT SANTA ELISABETH MEDAN TAHUN 2023

Knowledge is one of the main sources of progress or failure of a nation's civilization, and begins with people's attention to science. Knowledge is very important and needs attention to live a better life. Gastritis is a disease that is often found, even spread throughout the world and it is estimated that there are billions of sufferers of gastritis. This study aims to determine family knowledge about gastritis at Santa Elisabeth Hospital Medan in 2023. This study used a quantitative method where data was collected by distributing questionnaires with 18 statements so as to explore the knowledge of families of gastritis patients. This type of research is descriptive with a total population of 46 people. Sampling was carried out by purposive sampling technique and using the slovin formula, with a total of 32 respondents. The results showed that 18 respondents (58.1%) had good knowledge of the patient's family at Santa Elisabeth Hospital Medan, 11 respondents (35.5%) had enough knowledge, and 2 respondents (6.5%) had less knowledge. . It was concluded that the majority of families of gastritis patients at Santa Elisabeth Hospital Medan were categorized as "good" in knowing about gastritis. In this study, it was found that the knowledge of respondents was influenced by education and information obtained from the work environment, experience, social and mass media. With this knowledge, respondents can find out information about, understanding, etiology, classification, pathophysiology, risk factors, signs and symptoms, management and prevention of gastritis. It is hoped that the patient's family should share knowledge with the people around them and increase knowledge about gastritis and by seeking information from various available sources, or from people who have experience in preventing gastritis, can also through health workers in their area.

Inhibition of the Akt/NF-κB pathway is involved in the anti-gastritis effects of an ethanolic extract of the rhizome of Atractylodes macrocephala

Ethnopharmacological relevanceGastritis can lead to ulcers and the development of gastric cancer. The rhizome of Atractylodes macrocephala Koidz. (Asteraceae), a traditional Chinese medicinal herb, is prescribed for the treatment of gastric disorders, hepatitis and rheumatism. Its bio-active compounds are considered to be particularly effective in this regard. However, the molecular processes of the herb's anti-inflammatory activity remain obscure. This study elucidates a mechanism upon which an ethanolic extract of this herb (Am-EE) exerts anti-inflammation effects in RAW264.7 macrophage cells (RAW cells) stimulated by lipopolysaccharide (LPS) treatment and HCl Ethanol-stimulated gastritis rats. Aim of the studyTo investigate the anti-gastritis activities of Am-EE and explore the mode of action. Materials and methodsEthanol (95%) was used to prepare Am-EE. The quality of the extract was monitored by HPLC analysis. The in vivo effects of this extract were examined in an HCl Ethanol-stimulated gastritis rat model, while LPS-stimulated RAW cells were used for in vitro assays. Cell viability and nitric oxide (NO) production were observed by MTT and Griess assays. Real-time PCR was used to examine mRNA expression. The PGE2 ELISA kit was employed to detect prostaglandin E2 (PGE2). Enzyme activities and protein contents were examined by immunoblotting. Luciferase reporter gene assays (LRA) were employed to observe nuclear transcription factor (NF)-κB activity. The SPSS (SPSS Inc., Chicago, Illinois, United States) application was used for statistical examination. ResultsHPLC analysis indicates that Am-EE contains atractylenolide-1 (AT-1, 1.33%, w/w) and atractylenolide-2 (AT-2, 1.25%, w/w) (Additional Figure. A1). Gastric tissue damage (induced by HCl Ethanol) was significantly decreased in SD rats following intra-gastric application of 35 mg/kg Am-EE. Indistinguishable to the anti-inflammation effects of 35 mg/kg ranitidine (gastric medication). Am-EE treatment also reduced LPS-mediated nitric oxide (NO) and prostaglandin E2 (PGE2) production. The mRNA and protein synthesis of inducible cyclooxygenase (COX)-2 and NO synthase (iNOS) was down-regulated following treatment in RAW cells. Am-EE decreased NF-κB (p50) nuclear protein levels and inhibited NF-κB-stimulated LRA activity in RAW cells. Lastly, Am-EE decreased the up-regulated levels of phosphorylated IκBα and Akt proteins in rat stomach lysates and in LPS challenged RAW cell samples. ConclusionOur study illustrates that Am-EE suppresses the Akt/IκBα/NF-κB pathway and exerts an anti-inflammatory effect. These novel conclusions provide a pharmacological basis for the clinical use of the A. macrocephala rhizome in the treatment and prevention of gastritis and gastric cancer.

Knowledge Regarding Gastritis among Late Adolescence in Central Nepal

Gastritis is an inflammation or irritation of the lining of the stomach, can be a brief and sudden illness (acute gastritis), a long-lasting condition (chronic gastritis). Gastritis is the top most common cause of morbidity. This study is designed to find out knowledge regarding gastritis among late adolescence. A descriptive research design was used for the study. A semi-structured self-administered questionnaire was used to collect the data. Data were analysed and the descriptive and inferential test was applied by using SPSS. The study finding revealed eighty percent of the participants knew the definition of gastritis,66% on risk factors, 50% respondents on causes, 23.7% on sign and symptom,56.66% on management and 48.5% respondents on complications, and 66.55% on prevention of gastritis. The level of knowledge is average in causes, signs and symptoms, management and complications. More specifically,68% of respondents had average knowledge on gastritis and 4% had excellent knowledge. The level of knowledge of respondents is statistically significant with sex (p=0.021) and socio-economic status (p=0.008). The finding of the study revealed that knowledge on gastritis is average among the respondents. So the health education is needed for students to increase their knowledge.

The Effect of Health Education on Gastritis Prevention Behavior Among High School Students

Introduction: Gastritis is the inflammation of the gastric mucosa that is characterized by discomfort felt in the upper abdomen in addition to nausea, vomiting, a decreased appetite and headaches. The purpose of this study was to determine the effect of health education on the student’s behavior and on the prevention of gastritis. Methods: The design used was pre-experimental with a pretest-posttest design approach. The sampling technique used in this study was a total sampling technique resulting in 57 respondents. Before the intervention, the respondents filled in a questionnaire about the concept of gastritis. Furthermore, the respondents were given health education intervention material about gastritis in the form of leaflets containing information on the understanding, causes, signs and symptoms, risk factors and prevention. The intervention was only conducted for one session for 20-30 minutes per respondent. Following this, 1 hour later, the intervention was evaluated by giving the same questionnaire to the respondent to fill in according to the questions. The data analysis used a paired sample t-test ρ = 0,000.Results: The results showed an increase in behavior before and after the intervention related to preventing gastritis among the students. The results showed that 28 respondents (49.1%) with good behavior changed to 33 respondents (57.9%) who behaved well. Conclusion: The conclusion is that there is a significant influence from counseling on the prevention of gastritis among high school students.

Inhibition of H. pylori colonization and prevention of gastritis in murine model

Helicobacter pylori is a Gram-negative spiral bacterium that colonizes human gastric mucosa causing infection. In this study aiming at inhibition of H. pylori infection we made an attempt to evaluate immunogenicity of the total (UreC) and C-terminal (UreCc) fragments of H. pylori urease. Total UreC and its C-terminal fragment were expressed in E. coli. Recombinant proteins were analyzed by SDS-PAGE and western blot and then purified by Ni-NTA affinity chromatography. Female C57BL6/j mice were immunized with the purified proteins (UreC and UreCc). Antibody titers from isolated sera were measured by ELISA. Immunized mice were then challenged by oral gavage with live H. pylori Sydney strain SS1. Total of 109 CFU were inoculated into stomach of immunized and unimmunized healthy mice three times each at one day interval. Eight weeks after the last inoculation, the blood sample was collected and the serum antibody titer was estimated by ELISA. Stomach tissues from control and experimental animal groups were studied histopathologically. UreC and UreCc yielded recombinant proteins of 61 and 31 kDa respectively. ELIZA confirmed establishment of immunity and the antibodies produced thereby efficiently recognized H. pylori and inhibited its colonization in vivo. Pathological analysis did not reveal established infection in immunized mice challenged with H. pylori. The results support the idea that UreC and UreCc specific antibodies contribute to protection against H. pylori infections.

Suppressive Actions of Astragali Radix (AR) Ethanol Extract and Isolated Astragaloside I on HCl/ethanol-Induced Gastric Lesions

Roots of Astragalus membranaceus (A. membranaceus) (Astragali Radix, AR) has been used as a herbal medicine for gastrointestinal disorders in China, Korea, Japan, and other Asia countries. In this study we investigated the effects of the AR 70% ethanol extract and compounds isolated from AR on gastritis in rats and growth of human gastric cancer cells. AR 70% ethanol extract showed the potent acid-neutralizing capacities and partly cytotoxicity of Helicobacter pylori and human AGS gastric cancers cell. Astragaloside I and daucosterol, which were isolated from AR, significantly inhibited HCl/ethanol-induced gastric lesions. In addition, daucosterol increased the mucus content to almost the same as the positive control. The results of this study suggest that astragaloside I and daucosterol may be good candidates for the development of new drugs or neutraceuticals which can be used for the treatment or prevention of gastritis.