Prevalence Of Subclinical Endometritis Research Articles

Assessing Postpartum Reproductive Health via Endometrial Cytology and Body Temperature in Mehsana Buffaloes

The aim of this study was to investigate the effect of body temperature and endometrial cytology on postpartum reproductive health in Mehsana buffaloes. A total of twenty-six Mehsana buffaloes were used in this study. Per-rectal and ultrasonography examinations were used to observe the postpartum reproductive function of these buffaloes and on day 60 post natural service, the pregnancy was confirmed. Based on fertile estrus, buffaloes were retrospectively divided into two groups: Group I (buffaloes conceiving before 90 days) and Group II (buffaloes conceiving after 90 days). One week before parturition and four weeks after it, the rectal temperature was taken. The cytobrush technique was used to obtain endometrial samples for cytological tests on the 21st and 30th day postpartum. During the final week of pregnancy and the first, second, third, and fourth weeks’ post-partum, the mean rectal temperature (⁰F) varied from 101.7 ± 0.07 to 102.0 ± 0.06 in Group-I and 101.6 ± 0.07 to 102.1 ± 0.05 in Group-II. During the final week pre-partum and the first week post-partum, the mean rectal temperature (⁰F) was shown to be significantly (p<0.05) lower than it was during the second, third, and fourth weeks post-partum among the groups. On the 21st and 30th day postpartum during endometrial cytology, the percentage of neutrophils was 14.31 ± 1.56 and 12.23 ± 0.93 in Group I and 17.92 ± 1.51 and 15.08 ± 0.73 in Group II, respectively. On the 30th day, there was a significant difference (p<0.05) between the groups in Group II that had a greater mean percentage of neutrophils. When endometrial cytology was performed on buffaloes at various postpartum days, the mean percentage of lymphocytes, endometrial epithelial cells and large vacuolated epithelial cells revealed non-significant changes. On the 30th day, it was discovered that the prevalence of subclinical endometritis in Group I and Group II buffaloes was 7.69 and 23.07 percent, respectively. The postpartum first behavioural estrus and first fertile estrus occurred at 62.00 ± 4.34 and 65.31±4.45 days in Group I and 77.46±11.12 and 130.08±9.26 days in Group II buffaloes, respectively. Although, the average days for first behavioural estrus did not differ significantly between the groups the fertile estrus occurred significantly (P<0.05) delayed in Group II buffaloes.

Prevalence of subclinical endometritis in dairy cows that recovered after treatment of clinical endometritis with cephapirin and PGF2α

The aims of our study were to evaluate (1) the prevalence of subclinical endometritis (SE) in clinically cured cows after treatment of clinical endometritis (CE) with uterine cephapirin infusion or with systemic application of PGF2α and after self-healing, (2) the prevalence of SE in clinically cured cows in relation to the type of vaginal discharge. The study was conducted on 222 Polish Holstein Friesian cows with CE diagnosed by vaginoscopy and ultrasound. The animals were randomly allocated to one of three groups: Group 1 (n = 72): intrauterine cephapirin infusion; Group 2 (n = 73): single intramuscular injection of dinoprost; and Group 3 (n = 77): left untreated as a control. All cows were reexamined two weeks later (Exam 1). If there was still CE at this examination, these cows were treated using the same method as previously and were examined 2 weeks later (Exam 2). Cows still having CE at the second examination were examined two weeks later (Exam 3). Cows without signs of CE at Exams 1, 2 and 3 were considered cured, and endometrial samples from the uteri were collected by cytobrush to diagnose SE using cytological evaluation of PMN percentage. The threshold for SE was set at ≥ 5% PMNs. Two cows each from Groups 1 and 3 and three cows from Group 2 were not clinically recovered at Exam 3 and were excluded from the analysis. In total, SE was diagnosed in an average of 40.9% of clinically cured cows. The prevalence of SE in Group 1, 2 and 3 was 35.0%, 47.1% and 40.0%, respectively (p > 0.05). There were significantly higher (p < 0.05) SE cases after treatment of CE associated with purulent vaginal discharge than with mucopurulent discharge. In relation to the method of treatment, the prevalence of SE was significantly higher (p < 0.05) in cows treated with cephapirin with purulent vaginal discharge than with mucopurulent vaginal discharge. In conclusion, the study showed a high prevalence of SE in cows clinically cured after treatment of clinical endometritis with cephapirin or PGF2α and after self-healing. The prevalence of SE was significantly higher in cows with purulent vaginal discharge than with mucopurulent vaginal discharge. Therefore, in cows clinically recovered from clinical endometritis, the prevalence of SE should always be considered a negative consequence, and the resulting management of this uterine disease should be implemented at the herd level.

Clinical and morphological substantiation of the comparative effectiveness of the use of antibacterial drugs based on cefapirin in subclinical endometritis in cows

The article presents information on the prevalence of subclinical endometritis in cows, which were carried out on the basis of ZAO breeding farm "Povadino", as well as morphological changes in the mucous membrane of the uterus in this pathology. On the basis of the diagnosis, the animals were treated using various schemes with antibacterial drugs based on cefapirin. The studies were carried out on the basis of the Department of Anatomy and Histology of Animals named after Professor A.F. Klimov FGBOU VO MGAVMiB - MBA named after K.I. Scriabin, as well as at ZAO Povadino breeding plant, cytomorphological studies were carried out on the basis of the Chance Bio laboratory in the period from 2019-2021. According to the principle of analogs, 3 groups of animals with the manifestation of subclinical endometritis (15 animals in each) were formed. The animals of the first (experimental) group were injected with the drug "Mitrek"; the animals of the second (comparative) group - the drug "Metrikur", the animals of the third (control) group were injected with the drug "Tylosinocar" intrauterinely, according to the instructions. A comprehensive methodological approach was used, including clinical examination of animals, fine anatomical preparation, cytomorphological examination of cervical mucus, statistical analysis of the obtained digital data, light microscopy of histological sections stained by the classical technique with hematoxylin and eosin. To determine the extent and etiology of postpartum endometritis in black-and-white Holsteinized cows, obstetric-gynecological clinical examination was carried out, which was carried out according to the generally accepted method. In addition to general clinical methods for diagnosing this pathology, cytomorphological studies of 120 smears of cervical mucus were carried out to objectify the diagnosis. Based on the results of a comprehensive study aimed at identifying subclinical endometritis, it was found that this pathology is observed in 53.55% of the studied animals, macro- and morphological changes in the uterus with subclinical endometritis were revealed. endometrium, due to the influence of pathogenic microflora: Staphilococcus aureus, Escherichia coli, Streptococcus viridans group. And also, based on the results of the studies performed, a comparative assessment of the therapeutic efficacy of drugs based on cefapirin was carried out.

Changing views on the role of the uterus in post-partum reproductive function in beef cows1.

The paradigm for post-partum management of well-being and reproductive function in beef cows is changing. For many decades, research focused on the hypothalamic-pituitary-ovarian axis in attempts to manage beef cows to resume estrous cycles more quickly, because uterine involution was not considered a factor that was limiting to reproductive function in post-partum beef cows (Kiracofe, 1980). Questions remained, however, about the prevalence of subclinical endometritis in beef cows, and about the impact of sub-clinical reproductive tract health on subsequent reproductive performance and well-being (McNeel et al., 2017a). In a pair of papers published in this issue of the Journal of Animal Science, Ault and colleagues (Ault et al., 2019a,b) investigated the uterine and vaginal microbiome in post-partum beef cows using next generation sequencing and demonstrated that a less diverse uterine microbiome in the post-partum interval close to breeding was associated with a decreased risk of conception to timed artificial insemination. A similar result was reported for the vaginal microbiome of post-partum dairy cows (Bicalho et al., 2017). Beef cows, therefore, may not have as many cases of clinical metritis, but some of the same components of post-partum reproductive health may be influencing post-partum re-breeding efficiency in beef cows as in dairy cows. The results of studies examining the influence of endometrial cytology on reproductive performance in post-partum beef cows have been mixed (Santos et al., 2009; Ricci et al., 2017); however, the papers by Ault and colleagues indicate that the application of next generation sequencing technologies may allow more accurate determination of the relationships between post-partum reproductive tract health and re-breeding efficiency.

Comparison between cytology and histopathology to evaluate subclinical endometritis in dairy cows

The aim of the present study was to compare endometrial cytology with histopathology to diagnose subclinical endometritis (SCE) in dairy cows. Endometrial cytology samples were collected from Holstein-Friesian cows (n = 32) just before slaughtering. Half of them were obtained by in vivo cytobrush (IV-CB), whereas the other half by in vivo low-volume lavage (IV-LVL). After slaughtering, reproductive tracts were collected, and the endometrium was sampled at eight locations. At each location, both a ex vivo cytobrush sample (EV-CB) and a tissue sample for histopathologic examination were taken. In the histopathology slides, polymorphonuclear (PMN) cell counts were differentiated as PMN cells in direct contact with the epithelial cells of the endometrium (PMN-EP), and PMN cells present in the deeper stratum compactum (PMN-SC). Summation of both countings was referred to as PMN-total. Pearson's correlation and Cohen's kappa coefficient were used to assess the correlation and agreement between both sampling methods (in vivo cytology [IV-CB and IV-LVL] with EV-CB and PMN-total). A Poisson mixed effect model was used to analyze the PMN cells' distribution. The prevalence of SCE was 18.75% (n = 6/32) for in vivo cytology. The SCE prevalence based on EV-CB analyses and on the assessment of PMN-total was determined both at the sample (n = 256) as well as at the cow level (n = 32): EV-CB 25% (n = 64/256) and 35.5% (n = 12/32), and PMN-total 37.11% (n = 95/256) and 59.38% (n = 19/32). Correlation and agreement between IV-CB and EV-CB were r = 0.81 and k = 0.97, whereas between IV-CB and PMN-total r = 0.15 and k = 0.23, respectively. In vivo low-volume lavage correlation and agreement were r = 0.52 and k = 0.66 with EV-CB, and r = 0.45 and k = 0.44 with PMN-total. Moreover, correlation and agreement between EV-CB and PMN-total were r = 0.60 and k = 0.50, respectively. More PMN cells (P < 0.05) were detected in PMN-SC when compared to PMN-EP and EV-CB. A higher SCE prevalence was found using histopathology, rendering the latter as a more sensitive method to diagnose SCE in comparison to in vivo and ex vivo cytology. Although cytology had low and/or moderate sensitivity to diagnose SCE when compared with histopathology, its specificity is 100%, implying that all cows that were indicated to suffer from SCE using in vivo cytology were confirmed to do so by histopathologic examination. There is an uneven distribution of PMN cells throughout the endometrium, generally more PMN cells being found in the deeper stratum compactum than in contact with the superficial layers of the endometrium.

Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis

Information on the prevalence of subclinical endometritis and its mechanism in repeat breeding cows is very limited. The aims of this study were: a) to evaluate the incidence of this disorder with cytobrush cytology b) to analyze mRNA expression of tumor necrosis factor alpha (TNFalpha) and inducible nitric oxide synthase (iNOS) in endometrial biopsy samples collected from repeat breeding cows with and without subclinical endometritis. Two experiments were carried out. In experiment 1,112 (12.4%) repeat breeding cows (inseminated at least 3 times and not pregnant) were selected out of 902 cows from 8 dairy herds. Cytobrush cytology was performed on these cows, using the threshold of 10% PMNs in uterine smears. The results showed that 45 out of the 112 cows (40.2%) were diagnosed as having subclinical endometritis. In experiment 2, uterine biopsy samples were taken from repeat breeding cows with subclinical endometritis (n = 10) and without this disorder (n = 10). Using reverse transcription-PCR, the mRNA expression of TNFalpha and iNOS was determined. A statistically significant increase in expression of both substances was measured in the group of cows with subclinical endometritis (p <0.05). These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFalpha and iNOS pathways in the regulation of this pathological condition.

Genomic variation in innate immunity and susceptibility to uterine diseases in Holstein cows

Fertility is crucial for efficient dairy production, and adequate uterine health is a key element that allows for rapid resumption of reproductive function after calving. The challenge of uterine contamination with microorganisms at parturition is unavoidable. Some cows respond to this challenge successfully; however, approximately 20% of cows will develop metritis or clinical endometritis. Subsequently, the prevalence of subclinical endometritis will range from 25% to 75%. Innate immunity provides the first line of defense against infection by eliciting host responses to invading pathogens without requiring prior exposure. This response depends mainly on the action of neutrophils (PMNs) and macrophages with pattern recognition receptors that recognize molecular ligands from microbial species, which then initiate signaling pathways that result in the release of pro-inflammatory cytokines. One important group of receptors is the toll-like receptors (TLR), which regulate the activation of both innate and adaptive immunity. Likewise, the initial defense of the endometrium against invading microbes is dependent on the innate immune system, including initial recognition by host TLR proteins. Genetic selection for increased disease resistance in cattle has been proposed, and current molecular technologies provide the opportunity for exploring genomic variation related to health. We hypothesized that naturally occurring variation within the bovine TLR genes may be associated with differential susceptibility to reproductive diseases in Holstein cattle. The objective of this study was to test for associations between bovine single nucleotide polymorphisms (SNPs) and insertion-deletion (indel) mutations occurring in 7 bovine TLR genes (TLRsl, 2, 4, 5, 6, 9, and 10) that are known to recognize bacterial ligands and uterine diseases in dairy cows including metritis (MET), clinical endometritis (CE), and cytologic endometritis (CYE).

Presence of Escherichia coli, Trueperella pyogenes, α-hemolytic streptococci, and coagulase-negative staphylococci and prevalence of subclinical endometritis

Subclinical endometritis (SCE) has a severe negative effect on reproductive performance of dairy cows. Different studies have shown a wide variety of cut points for the polymorphonuclear neutrophilic leukocyte (PMNL) percentage to define SCE and differing sampling times. Possible risk factors for SCE, such as low body condition score, hyperketonemia, and increased haptoglobin concentrations have been demonstrated. The aim of this study was to determine the relationship between bacterial findings in the uterus and PMNL in the early postpartum period and to compare 2 classification schemes for the diagnosis of SCE. Cytological and bacteriological samples from the uterus were taken from 149 cows at 2 consecutive times postpartum [10±1 and 24±1d in milk (DIM)] and additionally, 131 cows were sampled at 21 to 27 DIM. For sample collection, the cytobrush technique was used and vaginal discharge was examined by vaginoscopy. Polymorphonuclear neutrophilic leukocyte cut points were set at 5, 10, and 18%. Cows positive with α-hemolytic streptococci at 10±1 DIM had significant higher PMNL percentages at 21 to 27 DIM and greater median days to pregnancy (193.5d) than cows not infected (123.0d). Cows with Trueperella pyogenes at 10±1 DIM had higher odds (5.2) of having more than 18% PMNL at 21 to 27 DIM compared with cows without infection. Escherichia coli had no effect on the prevalence of SCE regardless of the cut point. Primiparous cows with more than 18% PMNL had greater median days to pregnancy (144.5 d) than cows with less than 5% (80.0d) or cows between 5 and 18% PMNL (68.0d). Cows between 5 and 18% PMNL or 10 and 18% PMNL showed the best reproductive performance. The results demonstrate that an early infection with α-hemolytic streptococci increased the PMNL percentage 2wk later and that 18% PMNL at 21 to 27 DIM was a predictive cut point for primiparous cows to diagnose animals with or without SCE.

The diagnosis and prevalence of subclinical endometritis in cows evaluated by different cytologic thresholds

The aims of our study were to determine (1) how the prevalence of cytologically determined subclinical endometritis varies when using three different cytological threshold ratios to categorize cows as either with or without endometritis, (2) how the number of animals categorized as having endometritis changes from the fourth to the sixth wk postpartum when using each threshold, (3) how subclinical endometritis influences the number of days open, and (4) how the results of cytological and bacterial examinations correlate. To answer these questions, 222 clinically healthy cows in two herds were examined in the fourth (Exam 1) and the sixth wk (Exam 2) postpartum, when endometrial surface scrapings for bacteriologic and cytologic examination were collected by cytobrush from their uterine horns. After each examination, all cows were categorized using three different thresholds: (1) > 18% polymorphonuclear leucocytes in Exam 1 and > 10% in Exam 2, (2) > 8% in both exams, and (3) > 5% in both exams. It was found that: (1) The number of cows categorized as having endometritis increased as the threshold was lowered, and ranged from 18.9% to 75.4% according to herd, time of examination, and the threshold used; (2) with all three thresholds and in both herds, the number of cows categorized as having endometritis in Exam 1 was approximately double that in Exam 2; whereas depending on the herd and the threshold used, 6.1% to 17.0% of the cows that were negative in the first exam were positive in the second, and 7.4% to 33.3% were positive in both exams; (3) cows were open for a significantly greater number of days if categorized as having endometritis with the first threshold in Exam 1 (mean ± SEM 151.5 ± 9.5 vs. 115.9 ± 7.8; P < 0.01), or with either the first or the second threshold in Exam 2 (mean ± SEM 155.0 ± 15.0 vs. 125.1 ± 6.6; P < 0.05); and (4) the most common bacteria were Streptococcus acidominimus and Escherichia coli, and the correlation between cytologic and bacteriologic findings was low (Φ = 0.08 to 0.17 for different tested thresholds). Subclinical endometritis seems to be associated more with the postpartum recovery of the endometrium than with bacterial infection.

Effect of prostaglandin F2α on subclinical endometritis and fertility in dairy cows

The objectives were to determine the effects of PGF2α treatment on the prevalence of subclinical endometritis (SCE) and fertility of dairy cows. A total of 406 Holstein cows (167 primiparous and 239 multiparous) from 5 herds were used. Uterine lavage for diagnosis of SCE, PGF2α treatment, evaluation of body condition scores (BCS), and collection of blood samples for estrous cyclicity determination were performed at 21, 35, and 49 d in milk (DIM). Polymorphonuclear cells (PMN) were quantified and thresholds for diagnosing SCE were selected by receiver operating characteristics analysis. Cows classified as having SCE at 35 DIM (≥6.5% PMN) and 49 DIM (≥4.0% PMN) had increased time to pregnancy; however, cows classified as having SCE at 21 DIM (≥8.5% PMN) did not. Median days to pregnancy were delayed by 30 (151 vs. 121 d) and 40 (169 vs. 129) d for cows classified as having SCE at 35 and 49 DIM, respectively. Treatment with PGF2α did not affect the prevalence of SCE either at 35 (37.9 vs. 38.4%) or at 49 DIM (34.0 vs. 40.4%). Treatment with PGF2α did not affect time to first insemination (AI; median 76 DIM for cows treated with PGF2α; 79 DIM for control. Nonetheless, PGF2α treatment increased pregnancy to first AI in all the cows (35.5 vs. 24.1%) and hazard ratio (HR) of pregnancy in cows with BCS ≤2.5 when all of the cows were evaluated (HR = 1.5; 95% confidence interval; CI = 1.1 to 2.0) and when only cows without SCE were evaluated (HR = 1.8; 95% CI = 1.2 to 2.7). Treatment with PGF2α did not affect the hazard of pregnancy in cows with SCE at 49 DIM (HR = 0.9; 95% CI = 0.6 to 1.3). In these farms, treatment with PGF2α did not affect SCE or time to first insemination, but did increase first-service pregnancy per AI and decreased time to pregnancy in cows with low BCS.