Background: The overall weighted prevalence of depressive disorders is 2.7%, Generalized Anxiety Disorder (GAD) is 0.6%, Post-Traumatic Stress Disorder (PTSD) is 0.2% whereas the prevalence of Alcohol Use Disorders is 4.6%. Objectives: To assess the prevalence of moderate-to-severe stress in patients attending homeopathic general outpatient department (OPD) of five hospitals in three months by using Patient Stress Questionnaires (PSQ). Design: It was a multicentric, cross-sectional observational study. Setting: The present study was conducted among patients attending five homeopathic general OPD from 2016 Jul 1 to 2016 Sep 30. The period of recruitment was for three consecutive months. Patients: The study population was selected from the population on the basis of judgmental sampling who had attended these five centers for three months consecutively. Measurements: The PSQ consisted of previously validated set of total 30 self-administered questions. Results: In the present study, prevalence of moderate-to-severe stress in Patients Health Questionnaire-9 (PHQ-9), GAD-7, Physical Care Post-Traumatic Stress Disorder (PCPTSD) and Alcohol Use Disorder Identification Test (AUDIT) scale was 68.6%, 63.3%, 40.3% and 10.8%, respectively. Limitations: There may be response bias and recall bias which cannot be ruled out. Conclusion: The compositeness of PSQ may be used as it caters the occurrence of stress in an individual in its holistic form. Keywords: prevalence, stress, Patients Health Questionnaire (PHQ-9), Generalized Anxiety Disorder ( GAD-7), Physical Care Post-Traumatic Stress Disorder ( PCPTSD), Alcohol Use Disorder Identification Test ( AUDIT), homeopathic dispensaries Cite this Article Gurudev Choubey, Abhiram Banerjee, Shrabani Raymandal, Jogendra Singh Arya, Amitava Poddar. Prevalence of Stress in Patients Attending Homeopathic General Outpatient Department in Government Dispensaries at Siliguri, West Bengal, India: A Multicentric Cross-Sectional Observational Study. Research & Reviews: A Journal of Bioinformatics . 2020; 7(1): 14–22p.