Plant pathogenic nematodes pose a significant threat to banana production, causing symptoms such as root lesions, stunted growth, and reduced yield. A comprehensive study in Bangladesh’s Sylhet district aimed to assess nematode infestations in banana fields, involving interviews with farmers, soil sample collection, and microscopic examination. Results revealed the presence of multiple nematode species, with Helicotylenchus spp. being the most abundant. PCR analysis confirmed R. similis infestation in 35% of fields, highlighting its prevalence. Monoculture practices were observed among farmers, exacerbating nematode infestation risks. Limited communication between farmers and agricultural offices contributes to unawareness of nematode infestations. Country-wide studies and improved communication are crucial for implementing effective nematode mitigation strategies and safeguarding banana yield.