The aim of this study is to assess the knowledge, and treatment of MIH among dentists in Jordan. An online questionnaire using Google Forms was sent to the dentists who were registered with the Jordan Dental Association and worked in Jordan through social media groups. The questionnaire consisted of three sections, sociodemographic characteristics, the knowledge of dentists about the aetiology, diagnosis, and prevalence of MIH, and treatment options and barriers for treating MIH. Descriptive statistics with chi-squared test was used, and level of statistical significance was set to 5%. A total of 388 dentists had responded to the questionnaire (response rate 24.25%), (80.7%) reported that they have come across MIH cases during their practice. Yellow/brown demarcations were reported as the most common presentation of MIH (45.6%). All paediatric dentists were confident in diagnosing MIH, this was statistically higher than that reported by general dentists (56.8%) and dentists of other specialities (60.2%), P = 0.000. Pre-formed metal crowns were the treatment of choice for (57.2%) of participant, followed by composite restorations (52.3%). Child behaviour (56.2%), and late acknowledgment of the defect by the parents (46.1%) were the most encountered barriers for treatment. Molar incisor hypomineralisation is a common condition that is encountered frequently by general dental practitioners as well as dental specialists, nevertheless most dentists were not aware about the prevalence of MIH in Jordan and indicated their need to improve their approach regarding MIH diagnosis and treatment.