Prevalence Of Mixed Infections Research Articles

High prevalence of Helicobacter pylori mixed infections identified by multilocus sequence typing in Ningbo, China.

This study used multilocus sequence typing (MLST) to investigate the prevalence of Helicobacter pylori (H. pylori) mixed infections and H. pylori mixed infections involving unrelated strains; and determined the phylogeographic groups of H. pylori recovered from patients in Ningbo, China. A total of 156 H. pylori isolates were obtained from a convenience sample of 33 patients with culture-positive H. pylori infection. MLST was used to classify 150 H. pylori clinical isolates and 12 methodological control strains (6 clinical isolates and 6 strains of American Type Culture Collection H. pylori) into 43 and 12 sequence types (STs), respectively. In this study, 246 new alleles and 53 new STs were identified by MLST. The prevalence of mixed infections was 41% (11/27). The prevalence of H. pylori mixed infections involving unrelated strains was 46% (5/11) and the prevalence of H. pylori mixed infections involving completely unrelated strains (strains with all 7 housekeeping genes different) was 36% (4/11). A phylogenetic tree was created to determine the evolutionary relationships between different strains. The STs in this study were clustered within the hspEAsia subgroup (98%) and hpEurope group (2%). H. pylori mixed infections were common in Ningbo, China. The H. pylori isolates belonging to the hpEurope group were recovered from three different biopsy samples in a native Chinese patient. Most of H. pylori strains colonizing the antrum, corpus, and duodenum bulb were homologous.

Prevalence of ovine theileriosis in Mosul city, Iraq

The present study aimed to determine the prevalence of ovine theileriosis (OT) in sheep in Mosul city, Iraq using microscopic examination (ME) of the blood smears stained with MGG- Quick stain and conventional polymerase chain reaction technique (c-PCR) to compare between c-PCR technique and ME as techniques for the diagnosis of disease, and to investigate the pattern and type of infections based on multiplex polymerase chain reaction technique (m-PCR). From October 2021 to May 2022, one-handed eighty-five Blood samples were drawn randomly from sheep in various regions of Mosul city. The overall prevalence of OT was 42% (22.7 out of 185) and 52.4% (97 out of 185) using microscopic examination and c-PCR technique, respectively. A slight agreement was observed between ME of blood smears and c-PCR technique according to Kappa value 0.190, with low sensitivity, specificity, and accuracy of ME method was 30%, 88.6%, 58.4%, respectively, compared with c-PCR technique. The prevalence of mixed infection 22.7% and single infection with T. lestoquardi 20% were significantly higher (P<0.05) than single infection with T. ovis 9.7%. This study concludes that OT is widespread in Mosul city, Iraq, and the c-PCR technique is more reliable and suitable for detecting Theileriainfection in sheep than the ME method.

Quantitative PCR Effectively Quantifies Triazole-Susceptible and Triazole-Resistant Aspergillus fumigatus in Mixed Infections.

Increasing resistance to triazole antifungals in Aspergillus fumigatus is worrisome because of the associated high mortality of triazole-resistant A. fumigatus (TRAF) infections. While most studies have focused on single triazole-susceptible (WT) or TRAF infections, reports of TRAF cases developing mixed WT and TRAF infections have been described in several studies. However, the prevalence of mixed infections and their responses to current recommended therapies are unknown and could be inappropriate, leading to poor clinical outcomes. To address the urgent need for tools to diagnose, monitor disease development and therapy efficacies in mixed infection settings where quantification of WT versus TRAF is key, this study developed a novel qPCR assay to differentiate WT and TRAF harboring the cyp51A-TR34/L98H mutation. The proposed assay successfully quantified A. fumigatus and discriminated TRAF-TR34 in vitro and in vivo, which was achieved by increasing the yield of extracted DNA through improved homogenization and specific primers targeting the WT-sequence or TR34-insertion and a TaqMan-probe directed to A. fumigatus. The here-developed qPCR assay overcomes sensitivity issues of methodologies such as CFU counts, providing specific, reproducible, and reliable quantitative information to study and follow up the (interplay and individual) effects of mixed A. fumigatus infections on disease development and treatment responses.

Factors Associated with Amoebic Dysentery in Captive Non-Human Primates of The Mefou Primate Sanctuary

Primates are known to harbour different gastrointestinal parasite species that affect their survival and reproductive activity. Entamoeba histolytica infects humans predominantly as well as non-human (NH) primates causing amoebiasis. Amoebic dysentery is common amongst non-human primates in captive sites. The purpose of the study was to evaluate the risk factors associated with the prevalence of amoebic dysentery in captive NH primates of the Mefou Primate Sanctuary (MPS) in Cameroon. The faecal samples of 277 NH primates were analysed by qualitative (direct smear and evergreen kit sedimentation technique) coprology from January to July 2019. Factors such as habitat type, keeper’s age and level of education were assessed to evaluate their effect on the prevalence of amoebic dysentery. The prevalence of Entamoeba histolytica was 46.2% (P=0,079 and χ2=18.13). E histolytica was the most predominant parasite species detected and it was present in all instances of mixed parasite infections. Amongst the NH primates positive for E. histolytica, 28 suffered from amoebic dysentery giving a prevalence of 21.9% and one of the infected primates (Cercopithecus pogonias) actually died from amoebic dysentery. The prevalence of mixed infections was significantly lower amongst NH primates living in outdoor enclosures compared to those living in other habitat types (P=0.015, χ2=10.46). The rate of re-infection was significantly higher amongst NH primates under the care of keepers with the lowest (primary school) level of education (P=0.001, χ2=13.09) on one hand and of older keepers (≥50s) (P = 0.008, χ2=13.708) on the other hand. This study shows that amoebic dysentery is a cause for a major concern in the MPS. The housing conditions of the NH primates at the sanctuary, the keeper’s age and level of education were important factors that influenced the prevalence of amoebic dysentery. Adequate measures should be put in place to address the situation.

The Comparison of Real-time-PCR-HRM and Microscopy Methods for Detection of Mixed Plasmodium spp. Infections in Laghman Province, Afghanistan.

Laghman province, in the east of Afghanistan, is one of the most malaria endemic regions with prominence of Plasmodium vivax and Plasmodium falciparum. So far, no study has been conducted to investigate the extent of mixed infections in this area. In this study, we aimed to evaluate the prevalence of mixed infections of malaria in Laghman province by using a new and highly sensitive molecular method (real-time polymerase chain reaction high resolution melting analysis) and compare its results with microscopically confirmed cases of malaria. In general, 347 infected individuals have been referred to Khalwati laboratory that is located in the center of Laghman province from May to November of 2018. Microscopic analysis was performed on prepared thick and thin blood films under ×100 lens with oil immersion. The real- time-PCR-HRM assay was performed using an ABI 7500 Fast Real-time PCR system. In a microscopic examination, out of 347 patients referred to the Center, 267 (76.94%) cases were detected to be Plasmodium vivax, 79 (22.76%) Plasmodium falciparum and 1 (0.28%) case of mixed of two species. However, by using Real-time PCR-HRM technique, 249 (71.75%) were detected Plasmodium vivax, 79 (22.76%) were Plasmodium falciparum and 19 (5.47%) were mixed of two species. Our result indicated that the Real-time PCR-HRM method is more accurate and more reliable than microscopic method in the diagnosis of malaria mixed infections.

Application of MIRU-VNTR on smear slides: a shortcut for detection of polyclonal infections in tuberculosis patients.

Mixed (polyclonal) infections are one of the main problems in tuberculosis (TB) management. The best available method for detecting polyclonal infections in TB is mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR). According to multiple studies, MIRU-VNTR method can be applied to detect TB-related polyclonal infections in sputum samples or cultures. Setup of MIRU-VNTR on smear slides can be an efficient approach, regardless of the limitations of cultures and sputum samples in many laboratories. The present study aimed at investigating the diagnostic potential of MIRU-VNTR on smear slides in detecting mixed infections. Ziehl-Neelsen-stained microscopic slides were prepared from 14 clinical specimens. For amplifying 24 MIRU-VNTR loci, PCR assay was performed on the smear slides, clinical specimens, and cultures. Based on the 24-locus MIRU-VNTR analysis, polyclonal infections were reported in 42.85% of smear slides, while the corresponding rate was estimated at 57.1% (8/14) in the clinical samples. In the corresponding cultures, the rate of mixed infection was 7.14% (1/14). Use of smear slides can be a safe option for transferring clinical specimens between environmental and reference laboratories. Considering their significant impact on TB treatment, it is essential to diagnose mixed infections in low-resource countries with a high prevalence of mixed infections. The present findings show that direct MIRU-VNTR on smear slides can be conveniently used for the detection of mixed infections.

High prevalence of norovirus and rotavirus co-infection in children with acute gastroenteritis hospitalised in Brazzaville, Republic of Congo.

To evaluate the clinical severity of diarrhoea associated to viral co-infection in children with acute gastroenteritis. About 461 children under five years hospitalised with acute diarrhoea (266 males and 187 females) were enrolled in the study. Using stool samples, rotavirus and adenovirus infections were investigated by ELISA, and norovirus infections by nested duplex RT-PCR. We assessed social, demographic, clinical and behavioural conditions that might influence the occurrence of rotavirus, adenovirus and norovirus infections. Mono-viral infection was detected in 49% and mixed viral infection in 12% of patients. The prevalence of mixed infection was neither dependent on age nor sex. Three samples were infected with all three viruses. A significant association was found between fever (axillary temperature>37.5°C) and rotavirus-norovirus dual infection (aOR (CI 95%) = 2.1 (1.14-3.84), P=0.016; aOR (CI 95%) = 0.37 (0.19-0.73), P=0.004). Mixed infection was the most common during the dry season from June to October (71.4% versus 54.7%, P=0.023). Co-infection with both rotavirus and norovirus is common in under-five hospitalised children but does not contribute to the severity of the disease.

Aetiology and prevalence of mixed-infections and mono-infections in non-gonococcal urethritis in men: a case-control study

ObjectivesChlamydia trachomatis (CT) and Mycoplasma genitalium (MG) cause the majority of non-gonococcal urethritis (NGU). The role of Ureaplasma urealyticum (UU) in NGU is unclear. Prior case–control studies that examined the...

Effect of mixed infection on TB dynamics

Poor living conditions, overcrowding and strain diversity are some of the factors that influence mixed infection in Tuberculosis (TB) at the population level. We formulate a mathematical model for mixed infection in TB using nonlinear ordinary differential equations where such factors were represented as probabilities of acquiring mixed infection. A qualitative analysis of the model shows that it exhibits multiple endemic equilibria and backward bifurcation for certain parameter values. The reactivation rate and transmission rate of individuals with mixed infection were of importance as well as the probabilities for latent individuals to acquire mixed infection. We calculate the prevalence of mixed infection from the model and the effect of mixed infection on TB incidence, TB prevalence and Mycobacterium tuberculosis (MTB) infection rate. Numerical simulations show that mixed infection may explain high TB incidences in areas which have a high strain diversity, poor living conditions and are overcrowded even without HIV.

Prevalence and Risk Factors Associated with Haemoparasitosis in Village Chickens (Gallus Gallus Domesticus) in Gombe State, Nigeria

The present study was conducted between November, 2016 and September, 2017, aimed to determine the prevalence and risk factors associated with haemoparasitosis in village chickens in Gombe State, Nige ria. Blood samples were collected from a total of 1820 village chickens and Giemsa stained thin blood smears were microscopically examined for the presence of haemoparasites. Results revealed an overall prevalence of 19.6% of three genera of avian haemopar asites in a single and mixed infection. Amongst the haemoparasites encountered, Plasmodium (13.9%) was the most prevalent followed by Haemoproteus (2.6%) and Leucocytozoon (0.4%) in single infection, while the prevalence of mixed infection with Plasmodium + Haemoproteus was 2.6%. Higher prevalence rates were recorded in cocks (28.5%) compared to hens (8.9%), as well as in adults (23.0%) compared to the growers (11.0%) village chickens. The prevalence rates was found to be higher in the rainy (39.3%) compare d to cold dry (12.5%) and hot dry (7.7%) seasons of the study period. The difference in prevalence of haemoparasites among sex, age groups, and season was statistically significant (&lt;0.0001). In conclusion, haemoparasites exist among village chickens flock s in Gombe State, Nigeria probably due to the presence of vectors and inadequate practice of biosecurity measures. It is therefore, recommended that awareness should be created on the prevalence of haemoparasites, further researches involving molecular cha racterization of haemoparasites should be carried out and village chicken farmers should be educated on the need for maintenance proper biosecurity measures on their farms.

Diversity and evolution of anuran trypanosomes: insights from the study of European species

BackgroundAmphibian trypanosomes were the first ever described trypanosomatids. Nevertheless, their taxonomy remains entangled because of pleomorphism and high prevalence of mixed infections. Despite the fact that the first species in this group were described in Europe, virtually none of the trypanosomes from European anurans was analyzed using modern molecular methods.MethodsIn this study, we explored the diversity and phylogeny of trypanosomes in true frogs from Europe using light microscopy and molecular methods.ResultsA comparison of observed morphotypes with previous descriptions allowed us to reliably identify three Trypanosoma spp., whereas the remaining two strains were considered to represent novel taxa. In all cases, more than one morphotype per blood sample was observed, indicating mixed infections. One hundred and thirty obtained 18S rRNA gene sequences were unambiguously subdivided into five groups, correspondent to the previously recognized or novel taxa of anuran trypanosomes.ConclusionsIn this work we studied European frog trypanosomes. Even with a relatively moderate number of isolates, we were able to find not only three well-known species, but also two apparently new ones. We revealed that previous assignments of multiple isolates from distant geographical localities to one species based on superficial resemblance were unjustified. Our work also demonstrated a high prevalence of mixed trypanosome infections in frogs and proposed a plausible scenario of evolution of the genus Trypanosoma.

The burden of tick-borne diseases in the Altai region of Russia

This article presents the results of a comprehensive survey of the burden of tick-borne infectious diseases (TBIDs) in the Altai region of Russia. Official data for TBID incidence were analyzed and 201 samples from patients with suspected TBID were studied. Furthermore, questing ticks and ticks recovered from humans were examined to estimate prevalence of TBID-causative agents. The Altai region was determined to have a heightened risk for TBIDs in Russia. The most epidemiologically significant tick-borne illness in this area is spotted fever group rickettsiosis, while nationally in Russia, the leading TBID is Lyme borreliosis. The prevalence of mixed infection was 12.4% among the studied cases. Additionally, the prevalence of poorly studied pathogens − Kemerovo virus (KEMV) and Rickettsia tarasevichiae − in ticks from the Altai region was determined.

Drug resistance mediating Plasmodium falciparum polymorphisms and clinical presentations of parasitaemic children in Uganda

BackgroundPlasmodium falciparum genetic polymorphisms that mediate altered drug sensitivity may impact upon virulence. In a cross-sectional study, Ugandan children with infections mutant at pfcrt K76T, pfmdr1 N86Y, or pfmdr1 D1246Y had about one-fourth the odds of symptomatic malaria compared to those with infections with wild type (WT) sequences. However, results may have been confounded by greater likelihood in those with symptomatic disease of higher density mixed infections and/or recent prior treatment that selected for WT alleles.MethodsPolymorphisms in samples from paired episodes of asymptomatic and symptomatic parasitaemia in 114 subjects aged 4–11 years were followed longitudinally in Tororo District, Uganda. Paired episodes occurred within 3–12 months of each other and had no treatment for malaria in the prior 60 days. The prevalence of WT, mixed, and mutant alleles was determined using multiplex ligase detection reaction-fluorescent microsphere assays.ResultsConsidering paired episodes in the same subject, the odds of symptomatic malaria were lower for infections with mutant compared to WT or mixed sequence at N86Y (OR 0.26, 95% CI 0.09–0.79, p = 0.018), but not K76T or D1246Y. However, symptomatic episodes (which had higher densities) were more likely than asymptomatic to be mixed (for N86Y OR 2.0, 95% CI 1.04–4.0, p = 0.036). Excluding mixed infections, the odds of symptomatic malaria were lower for infections with mutant compared to WT sequence at N86Y (OR 0.33, 95% CI 0.11–0.98, p = 0.046), but not the other alleles. However, if mixed genotypes were grouped with mutants in this analysis or assuming that mixed infections consisted of 50% WT and 50% mutant genotypes, the odds of symptomatic infection did not differ between infections that were mutant or WT at the studied alleles.ConclusionsAlthough infections with only the mutant pfmdr1 86Y genotype were associated with symptomatic infection, this association could primarily be explained by greater parasite densities and therefore greater prevalence of mixed infections in symptomatic children. These results indicate limited association between the tested polymorphisms and risk of symptomatic disease and highlight the value of longitudinal studies for assessing associations between parasite factors and clinical outcomes.

High HCV subtype heterogeneity in a chronically infected general population revealed by high-resolution hepatitis C virus subtyping

ObjectivesThis study aimed to characterize the chronically infected general hepatitis C virus (HCV) population in Barcelona using a highly sensitive subtyping method that can identify the 67 recognized HCV subtypes and diagnose mixed infection by various genotypes/subtypes in a single individual. The resulting information has implications for selecting optimal direct-acting antiviral (DAA) treatment for each patient and establishing public healthcare policies in our setting. MethodsConsecutive HCV patients (treatment-naïve or interferon-based failures) attending Vall d'Hebron Hospital outpatient clinics from February 2015 to May 2016 (N=1473) were included in the study. Patient samples were characterized using HCV subtyping by next-generation ultra-deep pyrosequencing. ResultsThe following genotypes (G) were found: G1 (1126/1473 (76.4%)), G4 (145/1473 (9.8%)), G3 (135/1473 (9.2%)), G2 (51/1473 (3.5%)), and G5 (1/1473 (0.1%)). Twenty-two subtypes were seen: 1b (790/1473 (53.6%)), 1a (332/1473 (22.5%)), 3a (133/1473 (9.0%)), 4d (105/1473 (7.1%)), 4a (29/1473 (2.0%)), and 2c (25/1473 (1.7%)), with 16 low-prevalence subtypes accounting for the remaining 3.0% (44/1473). There was a worrisome 1.0% (15/1473) of mixed infections. G2 (51/1473 (3.5%)) showed a high level of heterogeneity. Analyses by age groups showed a predominance of G1b over G1a (428/506 (84.6%) vs. 24/506 (4.7%)) in patients born before 1950 (N=506/1473), and similar percentages of these subtypes in those born between 1951 and 1975 (N=834/1473) (315/834, 37.8% vs. 266/834, 31.9%) and after 1976 (N=133/1473) (47/133, 35.3% vs. 42/133, 31.6%). ConclusionsSubtype distribution showed a higher level of heterogeneity than was expected, particularly for G2. Prevalence of mixed infections was around 1%. HCV subtype distribution related to patient age group suggested that patients born from 1936 to 1975 in our setting should undergo screening for the infection. Next-generation sequencing enabled better classification of candidates for DAA-based treatment.

Prevalence of Mixed Infections among Females with Vaginitis in a Tertiary Care Center

Prevalence of Mixed Infections among Females with Vaginitis in a Tertiary Care Center

Clinical observation over the dynamic of the intrauteral mixed infection in the child of early age

Difficulty in diagnosis of the intrauterine infections (IUI) is associated with the low specificity of clinical manifestations and the multiple organ injury, prolonged persistence of the pathogen in the body, a significant prevalence of mixed infections and their frequent combination with the somatic disorders in newborns. The clinical case of mixed intrauterine infection in the child of early age is presented. The full-term boy was under the observation, he was born with severe anemic syndrome, indirect hyperbilirubinemia and hepatosplenomegaly. According to clinical observation and ultrasound of the abdomen the size of his liver and spleen increased progressively for six days after birth, it was accompanied by dynamic increase of total bilirubin due to its direct fraction, decrease of platelets count. ELISA-diagnostics of the child’s serum revealed the presence of the anti-toxoplasma and anti-cytomegalovirus antibodies. Taking into account obtained clinical and paraclinical data, the newborn child was diagnosed with intrauterine mixed infection with primary hematopoietic and liver damage (cholestatic-cytolytic variant). Anti-toxoplasma immunoglobulin was administered to child, but because of severe liver injury specific causal treatment of toxoplasmosis was postponed due to its hepatotoxicity. Later on anemic syndrome with indirect hyperbilirubinemia recurrently repeated in a child, it was dependent on transfusions but refractory to treatment. The presence of DNA of CMV and herpes virus type 6 was confirmed by PCR in the dynamic study of the five-month old child’s blood. Taking into account the obtained results of the examination, the child was diagnosed with severe congenital cytomegalovirus infection in the replication phase and comorbid congenital nonspherocytic hemolytic anemia of unknown origin with continuous crisis. The patient received anti-cytomegalovirus ganciclovir intravenously, antiherpetic valaciclovir orally. As a result of the treatment positive clinical dynamics was observed, which was accompanied by a decrease of the hepatolienal syndrome and gaining weight, but multiple red blood cell transfusions were not accompanied by significant or prolonged rise in hemoglobin levels. After the treatment twice done PCR child’s blood tests for CMV DNA were negative. Taking into account the crisis duration of the disease, splenectomy was discussed. The clinical case refers to the rare clinical diagnostic situations in medical practice when it is difficult to make a final conclusion about the etiology of the IUI. Verification of the intrauterine mixed infection was problematic in the neonatal period and during follow-up. The complexity of the diagnostic process was caused by the absence of the typical neurological symptoms. The diagnosis of mixed IUI was complicated by coexistence of the congenital nonspherocytic hemolytic anemia, the genesis of which could not be set, despite the study of enzyme activity in red blood cells, assessment of their osmotic resistance and Coombs tests. Damage of the erythroid stem hematopoiesis probably was caused by the infectious factors during the intrauteral period as well as by the hereditary pathology of the erythron.

Mixed HCV infection and reinfection in people who inject drugs--impact on therapy.

The majority of new and existing cases of HCV infection in high-income countries occur among people who inject drugs (PWID). Ongoing high-risk behaviours can lead to HCV re-exposure, resulting in mixed HCV infection and reinfection. Assays used to screen for mixed infection vary widely in sensitivity, particularly with respect to their capacity for detecting minor variants (<20% of the viral population). The prevalence of mixed infection among PWID ranges from 14% to 39% when sensitive assays are used. Mixed infection compromises HCV treatment outcomes with interferon-based regimens. HCV reinfection can also occur after successful interferon-based treatment among PWID, but the rate of reinfection is low (0-5 cases per 100 person-years). A revolution in HCV therapeutic development has occurred in the past few years, with the advent of interferon-free, but still genotype-specific regiments based on direct acting antiviral agents. However, little is known about whether mixed infection and reinfection has an effect on HCV treatment outcomes in the setting of new direct-acting antiviral agents. This Review characterizes the epidemiology and natural history of mixed infection and reinfection among PWID, methodologies for detection, the potential implications for HCV treatment and considerations for the design of future studies.

Investigation of gastrointestinal parasites of herbivores at Dhaka National Zoological Garden of Bangladesh

A total of 52 fecal samples were collected from 21 herbivores at Dhaka zoo during January to May, 2012 to investigate the prevalence of gastrointestinal parasites of herbivores. Overall prevalence of parasitic infection was 76.9%, of which 61.5% were positive for helminths and 55.8% were positive for protozoa. The identified parasites were Balantidium coli (55.8%), Paramphistomum sp. (36.5%), Fasciola sp. (19.2%), stomach worm (3.9%), Ascaris sp. (1.9%) and Strongyloides sp. (1.9%). Overall prevalence of mixed infection was 42.3%. Mixed infection was observed in the animals such as Butani cattle (Balantidium coli and Paramphistomum sp.), Giraffe (Balantidium coli and Paramphistomum sp.), Spotted deer (Balantidium coli, Paramphistomum sp. and Fasciola sp.), Sambar deer (Balantidium coli and Fasciola sp.), Horse (Balantidium coli and Ascaris sp.), Greater kudu (Balantidium coli and Paramphistomum sp.), Nil gai (Balantidium coli and stomach worm), Black wilde beast (Balantidium coli and Paramphistomum sp.), Impala (Balantidium coli and Strongyloides sp.), Hippopotamus (Balantidium coli and Paramphistomum sp.), Zebra (Balantidium coli and Paramphistomum sp.), Wilde beast (Balantidium coli and Paramphistomum sp.; Balantidium coli and Paramphistomum sp. and Fasciola sp.), One Horned Rhinoceros (Balantidium coli and Paramphistomum sp.), Common Eland (Balantidium coli and Paramphistomum sp.), Oryx (Balantidium coli and stomach worm). Diameter of ova or cyst was also measured. Egg per gram/ Cyst per gram of feces (EPG/ CPG) were ranged from 300-1200. Gastrointestinal parasites are highly prevalent in herbivores of Dhaka zoo. Strategic control measure is necessary to protect the endangered species from parasitic infestation. DOI: http://dx.doi.org/10.3329/jbau.v12i1.21245 J. Bangladesh Agril. Univ. 12(1): 79-85, June 2014

Molecular Determination of Mixed Infections of Dermatophytes and Nondermatophyte Moulds in individuals with Onychomycosis.

Abstract Background: Reports of mixed infections with NDMs and dermatophytes in onychomycosis are rare, possibly due to the inhibition of NDM growth during traditional culture. Objective: To determine the prevalence of mixed infections in onychomycosis using molecular identification. Methods: Molecular analyses were utilised to identify infecting organisms directly from at least two serial great toenail samples from each of the 44 subjects. Results: Mixed infections were present in 41% (18/44) of the subjects. A single co-infecting NDM was the most common mixed infection and was detected in 34% (15/44) of onychomycosis patients, with F. oxysporum present in 14% (6/44), S. brevicaulis in 9% (4/44), Acremonium spp in 2% (1/44), Aspergillus spp. in 4.5% (2/44) and Scytalidium spp. in 4.5% (2/44) of patients. Mixed infections with two NDMs were found in 7% (3/44) of the subjects. Conclusions: Mixed dermatophyte/NDM onychomycosis infections may be more prevalent than previously reported.

Prevalence of gastrointestinal helminthes in buffaloes in Barisal district of Bangladesh

A total 270 samples were collected from July 2012 to December 2012 from three upazilas (Barisal sadar, Bakergonj and Mehendigonj) of Barisal district to know the prevalence of gastrointestinal helminthes. Samples were collected in 10% neutral buffered formalin and shifted to Parasitology laboratory of the department of Pathology and Parasitology under the Animal Science and Veterinary Medicine (ANSVM) faculty of Patuakahli Science and Technology University. Samples were examined by direct smear method and simple sedimentation method of fecal sample examination technique. Among 270 samples 107 (39.6%) samples were found positive for gastrointestinal helminthes. Five types of helminthes were identified namely Fasciola gigantica (26.17%), Amphistomes (60.75%), Neoascaris vitulorum (1%), Schistosoma bovis (1%), Trichostrongylus axei (2%). The prevalence of mixed infection with Fasciola gigantica and Amphistomes was 9.34%. No cestode was found in this study. Comparative study among three different upazila shows highest prevalence in Barisal sadar (44%) and lowest in Mehendigonj (25%). Geographical location of different char island of Sadar upazila and season of sample collection may be the cause of highest prevalence of gastrointestinal helminthes in Sadar upazila of the district. Study period covered rainy season and part of winter season only. Highest parasitic ova were found in rainy season (44.32%) than winter season (29.41%). The age specific prevalence were 12.15%, 14.02%, 40.19%, 33.65% in 0-6 months, 7 months-2 years, 3 years- 6 years &amp; 7 years - above respectively. History of administering anthelmintic (Levamisole hydrochloride) to the calf may be the cause of lowest prevalence of parasitic infection at early age in the study area. In this study, highest number (60.75%) of Amphistomes was found among positive cases. Some important Amphistomes of buffalo are Paramphistomum, Cotylophoron, Gigantocotyle, Gastrothylax etc. are difficult to differentiate through fecal sample examination. So, to understand the detail epidemiology, at least a year study using gross morphological examination with molecular characterization of helminthes is needed.DOI:http://dx.doi.org/10.3329/bjvm.v11i2.19137Bangl.J.Vet.Med. (2013).11(2): 131-135