Prevalence Of Candida Species Research Articles

Epidemiology of Invasive Fungal Diseases: A 10-Year Experience in a Tertiary Pediatric Hematology-Oncology Department in Greece.

Although advances in the management of pediatric neoplasms have profoundly improved infectious disease outcomes, invasive fungal diseases (IFDs) remain a major cause of morbidity and mortality in children and adolescents with high-risk hematological malignancies. A retrospective study was conducted in the Pediatric Hematology-Oncology Department of the University General Hospital of Heraklion for 2013-2022 to estimate the prevalence and describe the clinical and epidemiological characteristics of IFDs for pediatric and adolescent patients with neoplasia. Demographic, clinical, and laboratory parameters were analyzed to identify risk factors for the development of IFD. The overall prevalence of IFDs was estimated to be 7.8% (12/154 patients) throughout the study. The mean age at IFD diagnosis was 9.8 years (SD 6.4 years). The most common IFD was possible/probable invasive pulmonary aspergillosis (IPA; in ≈50%), followed by candidemia/invasive candidiasis (in 44%). Candida parapsilosis was the most prevalent Candida species (4/6 events). Of interest, the majority (75%) of IFDs were breakthrough infections. Patients with increased risk for IFDs were those who were colonized by fungi in sites other than the oral cavity, hospitalized in the intensive care unit for >7 days, received >7 different antimicrobials in the last 3 months, or had severe neutropenia for >44 days. Two children out of a total of 12 with IFD died due to refractory disease or relapse (16.7%). More detailed and prospective epidemiological studies on fungal infections in pediatric patients with hematological or solid neoplasms can contribute to the optimization of prevention and treatment.

Second trimester vaginal Candida colonization among pregnant women attending antenatal care in Bukavu, Democratic Republic of the Congo: prevalence, clinical correlates, risk factors and pregnancy outcomes

IntroductionVaginal Candida colonization (CC) can lead to vulvovaginal candidiasis, the second most prevalent vaginal condition worldwide, and has been associated with adverse birth outcomes. However, no data on CC in the Democratic Republic of the Congo are available. We investigated the prevalence, Candida species, clinical correlates, risk factors and pregnancy outcomes in women with CC in the second trimester of pregnancy.Material and methodsIn Bukavu, the Democratic Republic of the Congo, pregnant women were recruited during antenatal care between 16 and 20 weeks of gestation from January 2017 to October 2017 and followed until delivery. Sociodemographics, sexual behavioral, hygienic and clinical characteristics, microbiological data and pregnancy outcomes were collected. Candida detection and speciation was performed with microscopy (Gram-stained smears and wet-mount) and/or quantitative PCR. Multivariate regression models were used to estimate the different associations with CC.ResultsThe prevalence of CC by wet mount, microscopy of Gram-stain smears and qPCR was 27.9%, 28.1% and 38.2%, respectively. C. albicans was the most prevalent Candida species (91.0%). Previous genital infections, an intermediate vaginal microbiota, bacterial vaginosis, and the use of pit toilets were risk factors for CC. Clinically, CC was associated with itching only. Women with CC had twice the odds for preterm birth, if Candida concentration was high, the odds were four times higher.ConclusionsIn Bukavu, the Democratic Republic of the Congo, the prevalence of CC was high and associated with microbiological and modifiable risk factors. Screening and treatment for CC during antenatal care should be investigated as a possible strategy to reduce preterm birth.

Enhanced Labor Management: Implementing WHO Recommendations in Oyo State Nursing Practice

Introduction: An increase in the incidence of Urinary tract infections due to Candida species has been observed. The higher rate of Candiduria due to Non albicans Candida is now being reported than due to Candida albicans.The emergence of drug resistance in Candida species has complicated the patient management. Aim: to observe the prevalence of Candida species in UTIs and their antifungal susceptibility pattern in a tertiary care hospital. Materials and Methods: An observational study was conducted fora period of three months in NIMS, Jaipur. Symptomatic patients belonging to all age groups were included in the study. Catheterized or clean catch midstream urine were centrifuged and the sediment was plated on Sabouraud’s Dextrose Agar slants with and without Chloramphenicol. A wet mount preparation was also made and looked for the presence of pus cells, yeast cells, pseudohyphae, etc. Yeast were identified by using germ tube, Chromogenic agar, chlamydospore formation and carbohydrate assimilation tests. Antifungalsusceptibility testing was performed on Muller Hinton Methylene Blue Agar. Result: The Candida species were isolated in only 24 (4.8%) samples. Only 8 cases yielded Candida albicans(33.33%). The highest cases were found to be of Candida tropicalis (n=14) while remaining two cases has growth of Candida krusai (n=1) and Candida glabrata (n=1). Keywords: WHO guidelines, labor management, and patient care & outcomes.

Prediction Model for Pregnancy Success in IVF Program in the Poor Prognosis Group

Introduction: An increase in the incidence of Urinary tract infections due to Candida species has been observed. The higher rate of Candiduria due to Non albicans Candida is now being reported than due to Candida albicans.The emergence of drug resistance in Candida species has complicated the patient management. Aim: to observe the prevalence of Candida species in UTIs and their antifungal susceptibility pattern in a tertiary care hospital. Materials and Methods: An observational study was conducted fora period of three months in NIMS, Jaipur. Symptomatic patients belonging to all age groups were included in the study. Catheterized or clean catch midstream urine were centrifuged and the sediment was plated on Sabouraud’s Dextrose Agar slants with and without Chloramphenicol. A wet mount preparation was also made and looked for the presence of pus cells, yeast cells, pseudohyphae, etc. Yeast were identified by using germ tube, Chromogenic agar, chlamydospore formation and carbohydrate assimilation tests. Antifungalsusceptibility testing was performed on Muller Hinton Methylene Blue Agar. Result: The Candida species were isolated in only 24 (4.8%) samples. Only 8 cases yielded Candida albicans(33.33%). The highest cases were found to be of Candida tropicalis (n=14) while remaining two cases has growth of Candida krusai (n=1) and Candida glabrata (n=1). Keywords: Infertility, prediction model, In Vitro Fertilization, Logistic Regression, Endometrial Thickness, Poor Prognosis, Clinical Pregnancy

Prevalence of Candida species and their antifungal susceptibility patterns among patients in tertiary care setting Butwal, Nepal: A cross-sectional study

Background: Candida species, commonly present in the human body, can result in severe infections. Non-albicans Candida (NAC) species, with increasing antifungal resistance, pose a growing concern. Aims and Objectives: This study aims to evaluate candidiasis prevalence and antifungal susceptibility in isolated Candida species among suspected patients at tertiary care hospitals in Butwal, emphasizing early identification and appropriate treatment to avoid unnecessary use of toxic antifungal drugs. Materials and Methods: In a descriptive study on 303 patients with clinical symptoms of Candida infections, specimens underwent direct microscopic examination and culture on Sabouraud dextrose agar. Species identification involved phenotypic methods such as chromogenic character on CHROMagar Candida media, germ tube examination, and microscopic characteristics. Subsequently, isolated species were tested for antifungal susceptibility using the disc diffusion method. Results: Among the 303 samples tested, 80 (26.4%) were positive for candidal infection. NAC species were the most commonly isolated, with Candida albicans and Candida krusei being the most virulent. The isolates exhibited the highest sensitivity to fluconazole (77.5%) followed by itraconazole (75%), whereas amphotericin B showed the lowest effectiveness with 63.75% resistance. Conclusion: The rising prevalence of NAC species, particularly their growing resistance to Amphotericin B, has become a significant concern, as these species are frequently detected in various clinical samples. These findings underscore the importance of diligent monitoring and judicious selection of antifungal agents to ensure effective treatment strategies.

Prevalence of Candida Species Among Unmarried Women Attending Lafia Primary Health Care Centers

One of the several normal-flora of an individual’s vaginal, oral cavity, gastrointestinal tract and mucosal membrane are Candida species. Candida have been associated with diverse diseases including life threatening invasive, superficial and hematogenic infections. This study determined the prevalence of Candida species amongst unmarried women attending Lafia Primary Health Care Centers. Forty high vagina swabs were collected from unmarried women. Isolation and identification of Candida species were performed according to standard procedures using Sabouraud Dextrose Agar, Gram staining and Germ tube test. 13 (32.5%) were positive for five different Candida species. C. albicans was the most prevalent (38.46%; 5/13) species while non-albicans Candida [C. glabrata (23.07%; 3/13), C. tropicalis (6.89%; 2/13), C. parapsilosis (3.44%; 1/13) and C. krusei (6.89%; 2/13)] accounted for 61.54% (8/13) of the total positive cases. Women aged 15-18 years and 23-26 years had the lowest and highest prevalence rates of 14.28% (1/7) and 42.10% (8/19) respectively. Candidiasis is a threat to global health and should be taken with all seriousness. The high candidiasis prevalence observed herein portrays a concern and calls for a continuous study of unmarried women attending Primary Healthcare Centers in Lafia. Furthermore, there should be an improvement in creating awareness of the opportunistic pathogenic characteristic of Candida species as normal flora and the effect they cause when there are hormonal imbalances caused by drug abuse, pregnancy, and poor dieting. The practice of good hygiene should be observed to prevent the spread of the infection.

Assessment of Proteinase and Phospholipases Enzymes Isolated from Pathogenic Candida species from Women Attending Antenatal Clinic at Mbeya Zonal Referral Hospital, Southern Highland Regions of Tanzania

Fungi are a global cause of vaginal infections, with vaginal candidiasis largely afflicting tropical regions including most parts of sub-Saharan Africa. In Tanzania, baseline studies have established the prevalence of Candida species among symptomatic and asymptomatic pregnant women in Dar es Salaam (Namkinga et al., 2005; Namkinga 2012) and in Mwanza (Mushi et al., 2019). However, no study that has been done to determine the above-mentioned enzymes form Candida species in Southern Highland Regions of Tanzania. Several factors have been reported to contribute to the virulence and pathogenicity of Candida, among others the production of extracellular hydrolytic enzymes, particularly phospholipase and proteinase. Therefore, this study was designed to investigate the in vitro production of phospholipases and proteinases enzymes from isolated pathogenic Candida spp from pregnant women.Of the 280 samples collected from symptomatic and asymptomatic pregnant women attending antenatal clinics at Mbeya Zonal Referral Hospital were tested for several microbiological methods such as the wet mount microscopic preparations (hanging drop) and the Gram stained smears for microscopy, cultures on Sabouraud’s dextrose agar and on Conidia enhancing medium (Corn meal agar), germ tube test, biochemical tests, sugar fermentation-assimilation tests and molecular test to characterize Candida species.Phospholipase production was performed in egg yolk medium while the production of proteinase was done in a medium containing bovine serum albumin. All analyses were performed in triplicates. The results showed that; out of the 280 tested, 155 (74.3%) isolates were phospholipase positive while 201 (96.6%) were positive for proteinase activity. C. albicans was the species with the highest number of positive isolates for proteinase and phospholipase 110 (95.6%), and most strains of C. albicans produce both enzymes (phospholipases and proteinases). The non-albicans Candida isolates were also producers of hydrolytic enzymes that, consequently, might be able to cause infections as favorable conditions arises.

Etiology and Antifungal Sensitivity Test in Otomycosis Caused by Candida Sp

Otomycosis is a common fungal infection of the external auditory meatus frequently diagnosed in otolaryngology outpatient clinics. Resistance to antifungals is currently a significant concern, with intrinsic and acquired resistance increasing among isolates that cause fungal infections. The purpose of this research was to identify Candida species causing otomycosis and determine the pattern of antifungal susceptibility among these Candida species. A prospective study was conducted in the Margono Soekarjo General Hospital and Department of Microbiology, Faculty of Medicine, Universitas Jenderal Soedirman Purwokerto, Indonesia, from April – September 2022. Forty-seven (47) clinical samples of otomycosis were collected from 41 patients and then isolated bedside on fungal culture media and was prepared on an object glass for direct microscopic examination of the specimens. Fungal identification was performed using 10% potassium hydroxide (KOH) to observe fungal elements. Samples were cultured on Saboraud dextrose agar (SDA) media with chloramphenicol and Czapek dox agar. The Germ Tube Test was used to identify Candida while yeast-specific identification and antifungal susceptibility assay using a rapid commercial kit was applied for specific identification of the fungus. Antifungal susceptibility patterns were obtained using the Integral System Yeast Plus (ISYP) media pack. Candida parapsilosis was the most prevalent Candida species discovered in this study, accounting for approximately 41.66%, which was followed by Candida tropicalis (25%) and Candida krusei (12.5%), whereas Candida albicans only accounted for 4.1% of the specimens. All Candida species were sensitive to flucitosine and ketoconazole, whereas the voriconazole sensitivity rate reached 96%. This study concludes that Candida parapsilosis is the most prevalent species of Candida in otomycosis, and that all Candida species are sensitive to Flucitosine, ketoconazole, and voriconazole.

The Prevalence of Candida Species among HIV-Positive Women in Aluu, Nigeria: A Call for Awareness

Background: The acquired immune deficiency syndrome (AIDS), which HIV brings on, is a major public health concern. The human immunodeficiency virus (HIV) is still a pandemic. Only 2.1% of HIV cases worldwide are recorded in Nigeria. Usually, under the control of the immune system, Candida infections may be dangerous for immunocompromised people, particularly those living with HIV/AIDS.
 Aim: At the Mbodo Health Centre in Aluu, Rivers State, Nigeria, this study aimed to assess the prevalence of Candida infections and their relationship to HIV-positive patients.
 Methods: Fifty HIV-positive women were chosen at random. Self-collected oral and vaginal swabs were used to gather data. To identify Candida species, biochemical testing, culture, and microscopy were used.
 Results: Eighty percent of the 50 individuals had positive candidiasis tests. Oral and vaginal mono/co-candidiasis were found to be common, with Candida albicans being the most common specie. Based on demographic parameters, statistical analysis did not show a significant difference in the prevalence of oral candidiasis. The frequency of vaginal candidiasis was much greater in those with secondary education and aged 31-40 years. The age group of 31 to 40 years old had a considerably greater co-occurrence rate of oral and vaginal candidiasis. Adult female HIV-positive patients at Mbodo Health Centre have a significant prevalence of candidiasis.
 Conclusion: The study sheds light on the distribution of Candida species and how demographic variables relate to them. These results highlight the significance of treating candidiasis as a serious health issue in people living with HIV. To improve overall health outcomes for HIV-positive people and reduce related risk factors, further research and treatments may be required.

Prevalence of Candida species and their Susceptibility to Triazoles in Clinical Isolates from a Tertiary Care Hospital

In the recent past, the incidence of Candidiasis has witnessed a concerning upsurge, resulting in a significant healthcare challenge. These infections are further exacerbated by factors like the widespread use of broad-spectrum antimicrobials, chemotherapy-induced neutropenia, and the presence of medical devices. The present study is designed to address the critical need for identifying the Candida species responsible for clinical infections and assessing their susceptibility to key antifungal drugs Fluconazole, Voriconazole, and Itraconazole. Two hundred clinical samples from Rohilkhand Medical College & Hospital, Bareilly were analyzed. Using Vitek-2 Compact (Biomerieux, France), the Candida spp. and the antifungal drug sensitivities were identified for Fluconazole and Voriconazole. E-test was done to identify Itraconazole sensitivity. This study found that C. albicans accounted for 21.5% while Non-albicans Candida (NAC) constituted 78.5%. Prolonged medication was the most common factor making susceptible for Candidiasis (43.5%), followed by indwelling biomedical devices (23%), Diabetes mellitus (16%), surgical causes (5.5%), trauma (5%), pregnancy (5%), and HIV (2%). Antifungal susceptibility testing showed that 68.5%, 72%, and 69.5% of Candida spp. isolates were sensitive to Fluconazole, Voriconazole, and Itraconazole, respectively. In conclusion, non-albicans Candida infections are increasing due to predisposing conditions, and some of these species are inherently resistant to the routinely used antifungal drugs. The study emphasizes the importance of identifying Candida spp. and their susceptibility to antifungals. This can limit the indiscriminate use of antifungal drugs, aid in selecting appropriate treatments, and reduce treatment costs, hospital stays, and patient morbidity and mortality.

The impact of increasing non-albicans Candida trends on diagnostics in immunocompromised patients

Invasive candidiasis (IC) represents a growing concern worldwide, with a considerable increase in non-albicans Candida (NAC) species. The study's primary goal was to determine if species identification by semi-nested PCR (sn-PCR) with primers for the five most prevalent Candida species is sufficient to deal with the current trends of Candida infections in cancer patients. Over one year, Candida isolates were collected from samples of patients with hematological and solid organ tumors in a single center. Species of Candida were identified by chromagar and multiplex sn-PCR using specific primers for Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei, and the Candida parapsilosis complex. Most Candida infection episodes are caused by NAC species (70.5% of 105 isolates). Rare species (14 isolates) accounted for 13.3% of isolates and were not identified by sn-PCR using the five most common Candida species primers. More than half of these rare species caused candidemia in cancer patients (57.1%; p = 0.011). The risk factor for candidiasis was recent surgeries (p = 0.020) in adults and chemotherapy in pediatric patients (p = 0.006). Prolonged hospitalization and genitourinary tract cancer were significantly associated with invasive infections (p = 0.005 and 0.049, respectively). Recent surgery was a significant risk factor associated with C. parapsilosis and C. glabrata infections (P = 0.038 and 0.003, respectively), while C. tropicalis was significantly more common in patients with hematological malignancies (P = 0.012). Techniques with a broader identification spectrum than the major five Candida species are crucial for the optimal management of cancer patients.

Paradigm Shift: Candida parapsilosis sensu stricto as the Most Prevalent Candida Species Isolated from Bloodstream Infections with Increasing Azole-Non-Susceptibility Rates: Trends from 2015-2022 Survey.

Candidemia is the fourth most common healthcare-related bloodstream infection. In recent years, incidence rates of Candida parapsilosis have been on the rise, with differences in prevalence and antifungal susceptibility between countries. The aim of the present study was to evaluate temporal changes in prevalence and antifungal susceptibility of C. parapsilosis among other species causing candidemia. All candidemia episodes from January 2015 to August 2022 were evaluated in order to depict time trends in prevalence of C. parapsilosis sensu stricto among all Candida species recovered from blood cultures as well as fluconazole- and voriconazole-non-susceptibility rates. Secondary analyses evaluated time trends in prevalence and antifungal non-susceptibility according to clinical settings. The overall prevalence of C. parapsilosis was observed to increase compared to the prevalence of other Candida species over time (p-trend = 0.0124). From 2019, the number of C. parapsilosis sensu stricto isolates surpassed C. albicans, without an increase in incidence rates. Overall rates of fluconazole- and voriconazole-non-susceptible C. parapsilosis sensu stricto were both 3/44 (6.8%) in 2015 and were 32/51 (62.7%) and 27/51 (52.9%), respectively, in 2022 (85% cross-non-susceptibility). The risk of detecting fluconazole- or voriconazole-non-susceptibility was found to be higher in C. parapsilosis compared to other Candida species (odds ratio (OR) = 1.60, 95% CI [1.170, 2.188], p-value < 0.0001 and OR = 12.867, 95% CI [6.934, 23.878], p-value < 0.0001, respectively). This is the first study to report C. parapsilosis sensu stricto as the most prevalent among Candida spp. isolated from blood cultures, with worrisome fluconazole- and voriconazole-non-susceptibility rates, unparalleled among European and North American geographical regions.

Prevalence of Candida Species Isolates in Patients with Urinary Tract Infection in Madinah, Saudi Arabia

INTRODUCTION: Urinary tract infections (UTIs) is considered one of the most prevalent infections that may lead to many renal complications or dysfunctions. They are responsible for almost 10% of all infections in Saudi Arabia, which makes them the second most common cause of emergency department admission. Bacteria are the most common pathogens associated with UTIs. Nevertheless, UTIs caused by fungi have also been reported. Among fungal infections, Candida spp. has been identified as the primary cause of UTI-related mycoses. &#x0D; &#x0D; OBJECTIVE: To assess the prevalence of Candida species isolated from adult patients in Madinah, Saudi Arabia.&#x0D; &#x0D; MATERIALS &amp;amp; METHODS: A retrospective cross-sectional study was performed using data collected from patients who visited King Fahad General Hospital in Madinah, Saudi Arabia. A total of 16803 urine bacterial cultures data were collected from January 2019 to October 2021. &#x0D; &#x0D; RESULTS: Among the 4281 positive urine cultures, 92% (n = 3937) showed bacterial growth, while 8% (n = 344) exhibited fungal growth. Female patients had a slightly higher percentage of fungal-positive cases (53%, n = 181) compared to males (47%, n = 163). Among different nationalities, Saudi citizens had the highest prevalence of positive fungal samples (64%, n = 220). Most positive cases (49%, n = 167) were isolated in 2019, with a decline in subsequent years. Non-Candida albicans spp. (86.3%, n = 297) were the most common fungal species, followed by Candida albicans (13.7%, n = 47).&#x0D; &#x0D; CONCLUSION: Despite considering bacterial UTIs to be more prevalent than UTI-related mycoses, it remains crucial to accurately identify the causative agent for proper diagnosis and treatment. UTI-related mycosis caused by non-Candida albicans spp. has significantly increased compared to Candida albicans. Thus, monitoring these trends over time can provide valuable insights for developing preventive strategies and optimizing treatment approaches.

Prevalence of Candida Species Isolated from Immuno-Compromised Patients

Background: Resistance to fungal infections is increasing worldwide, which is highly relevant in immunosuppressed individuals. Candida fungi infection constitutes one of the most common causes of fungal infections in such patients, and it can lead to complications and death. The current study sought to examine the prevalence and species diversity of Candida in samples received by the Microbiology Laboratory over a two-year period.&#x0D; Methods: The study involved 674 immunocompromised patients. Candida was isolated from clinical samples using wet mount, gram stain, and SDA culture. To further identify the organisms, several tests were conducted, including the germ tube test, cornmeal agar morphology, sugar assimilation, fermentation tests, and BACT/ALERT 3D.&#x0D; Results: Candida species were found to be present in 6.5% of the population. Candida tropicalis was found to be the most common isolate 20 (45.45%), followed by Candida albicans 9 (20.45%), Candida glabrata 7 (15.9%), Candida parapsilosis 4 (9.09%), and Candida krusei 4 (9.09%). Diabetes, leukaemia, chronic kidney disease, and AIDS were the most common predisposing factors for candidiasis.&#x0D; Conclusion: Men had a relatively high candidemia prevalence of 68.18%. Uncontrolled diabetes mellitus (DM) and chronic kidney disease (CKD) have been found to be the most common co-morbidities with candidemia in people aged 51 to 70.

Phenotypic and genotypic characterization of virulence markers and antifungal susceptibility of oral Candida species from diabetic and non-diabetic hemodialysis patients

BackgroundPatients with chronic kidney disease undergoing hemodialysis are often colonized by Candida species with high possibility of fungal infections. The purposes of this study were to determine the prevalence of Candida species, evaluate antifungal susceptibility profile, biofilm formation, proteinase and phospholipase activities, and the frequency of virulence genes in the Candida species isolated from the oral mucosa of hemodialysis diabetic (DM) and non-diabetic (non-DM) patients.MethodsThis study identified several species of Candida isolated from 69 DM and 58 non-DM patients on hemodialysis using phenotypic methods and PCR–RFLP technique. The identification of C. albicans and C. glabrata complex was performed by HWP1 gene and four oligonucleotides (UNI-5.8S, GLA-f, BRA-f, and NIV-f), respectively. Antifungal susceptibility to amphotericin B, fluconazole, itraconazole, voriconazole, and caspofungin was assessed according to CLSI M27-A3/S4. The biomass, metabolic activity of biofilm, proteinase (Prz), phospholipase (Pz), and molecular study for virulence genes were assessed using crystal violet, XTT assay, agar-based hydrolytic enzyme, and PCR technique, respectively.ResultsCandida prevalence was 44.9% with 47.8% and 41.4% among DM and non-DM patients, respectively (P = .045). The species identified were C. albicans (49.5%), C. glabrata (16.5%), C. tropicalis (12%), C. kefyr (8.8%), C. parapsilosis (6.6%), C. dubliniensis (3.3%), and C. lusitaniae (3.3%). The antifungal susceptibility profile showed that all Candida isolates were sensitive to amphotericin B, itraconazole, voriconazole, and caspofungin whereas fluconazole resistance was observed in 6.3% (MIC ≥ 64 μg/mL) of C. albicans and 6.6% of C. glabrata (MIC ≥ 64 μg/mL). The susceptible- dose-dependent rate was found in 10.5% of C. albicans. The Prz values of C. albicans ranged from 0.37 to 0.66 for the DM and 0.44–0.73 for the non-DM group (P < 0.05). The non-albicans Candida (NAC) species produced higher degree of biomass and metabolic activity compared to C. albicans (P < 0.05). Furthermore, significant (p < 0.05) correlations were detected between the biofilm formation with Prz values and fluconazole MICs. The most detected virulence factors were ALS3 and Sap5.ConclusionsThese results showed the importance of prevalence of NAC species in hemodialysis patients. Investigating antifungal susceptibility profile made a better understanding of the role of virulence markers in the pathogenesis of Candida strains.

Antifungal biomaterial for reducing infections caused by Candida albicans in edentulous patients.

Polymethylmethacrylate (PMMA) acrylic resins are used to make dentures for edentulous patients. To find out the prevalence of Candida species in patients with and without removable prostheses from a dental clinic in León, Guanajuato, as well as to assess the antifungal effect and biological behavior of an experimental PMMA with silver nanoparticles for its possible application in prostheses. To identify Candida species, smear samples were obtained from the palatal mucosa of 140 patients aged ≥ 60 years. The experimental PMMA with silver nnoparticles was placed in Candida albicans cultures, which were stained with the Live/Dead® kit for analysis under confocal microscopy; subsequently, it was implanted in Wistar rats in order to know its behavior in the surrounding tissues. Candida albicans was the most prevalent species in the evaluated patients, followed by Candida tropicalis and Candida krusei. The acrylic resin with silver nanoparticles significantly decreased the presence of Candida albicans. In the animal model, a discrete and controlled inflammatory reaction was found, which indicated biocompatibility of the acrylic resin that was used. It is possible for the nanostructured material with antifungal effect to be used in order to promote the reduction of oral Candida infections in edentulous patients.

Predominance of non-Candida albicans species oral colonisation among patients on anticancer therapy: findings from a cross-sectional study in Tanzania

ObjectivesThis study aimed to determine the oral carriage prevalence of Candida species and identify factors associated with the carriage of Candida species among patients with cancer on treatment.DesignA hospital-based cross-sectional...

Incidence and prevalence of oral candidal colonization in patients with cleft lip and palate.

This study aims to assess and compare the incidence and rate of oral candidal colonization in patients with cleft lip and palate and collate it with healthy individuals. The objectives of this study were to evaluate the factors that may affect the incidence, rate, and polycolonity of colonization of oral Candida species in patients with cleft lip and palate (CLP) as compared to healthy individuals. The current prospective study involved the collection and assessment of oral samples from 50 CLP patients and compared it with oral samples from 50 healthy individuals. Colonies of Candida albicans and other Candida species were identified by using germ tube test and differential chromogenic media. Assessment of the distribution trend of various Candida species and their variation based on age, gender, type of cleft, number of surgeries, and oral hygiene status were recorded. A highly significant (P value < 0.001) statistical difference was seen in the oral carriage rate of Candida species between the two groups with values more in the study group (60.75 ± 71.43) than in controls (1.62 ± 2.22). Individually comparing the different Candida species, significant difference in presence of C albicans (P value = 0.004), C parapsilosis (P value = 0.015), and C dubliensis (P value = 0.027) was seen as compared to other species. In the context of the type of cleft and the presence of Candida species Candida albicans was the most prevalent in patients with the cleft alveolus (60.0% n = 15), unilateral CLP (66.7% n = 8), and bilateral CLP (100.0% n = 3) respectively. Nonsignificant variation in prevalence was seen in the various fungal species based on an individual's gender and oral hygiene and the number of surgeries status was observed. The anatomical alteration in the oral cavity of patients with CLP contributes to the high incidence and prevalence of Candida species.

In Vitro Assessment of Conventional and Plant-derived Antifungal Agents against Candida Species Prevalence

In Vitro Assessment of Conventional and Plant-derived Antifungal Agents against Candida Species Prevalence

PROFILING AND SPECIATION OF CANDIDA SPECIES BY USING HICHROME AGAR FROM VARIOUS CLINICAL AND POSTMORTEM SAMPLES IN LIVESTOCK AND POULTRY

Animal Candidiasis is associated with oral and upper respiratory disease, pyothorax, ocular lesions, intestinal disease and urocystitis in dog and cats and causes arthritis in horses and mastitis and abortion in cattle. The present study was undertaken to determine the prevalence of Candida species among various clinical and post mortem samples of livestock and poultry. All samples were collected using aseptic precautions. After receiving in the Central University laboratory, the samples were inoculated onto both Blood agar and MacConkey agar, XLD agar and SDA agar and plates were incubated at 37 oC for 24 hours aerobically. The colonies appeared as smooth, pasty, opaque, white or beige were suspected as Candida colonies and Gram stain was done for confirmation. The speciation of the Candida isolates was performed by inoculating it on Hicrome Candida differential agar. The Hicrome agar was prepared as per the manufacturer’s instructions and incubated at 37 o C for 24 hours aerobically. The antibiotic sensitivity test was performed with Highrome agar plate. The pure culture of Candida albicans were streaked in hichrome agar plate and antibiotic disc were placed in appropriate distance and incubated at 37 oC for 24 hours aerobically. Biofilm study of yeast was performed with Sterile 96-well polystyrene plates which were inoculated with 200 µl bacterial suspension (105 CFU/ml) in BHI medium and incubated at 37 o C for 24 hrs without shaking. Species identification of Candida was done by the morphology and color of the colonies. The Candida albicans produce light green colonies, C. tropicalis metallic blue colonies, C.krusei produces purple fuzzy colonies, and C. glabrata white to cream-colored colonies. Out of 298 Candida isolates, C. Albicans was the most common species in 210 (70.46%) strains. The remaining 88 (29.5%) strains showed Non-albicans Candida. Out of 88 Non-albicans Candida isolates, Candida isolates, 20 were C. tropicalis (23%), 28 were C. glabrata (32%), 18 were Candida parapsilosis (20%) and 22 were C. krusei (25%) respectively (Table 1). Out of 298 isolates from oronasal swab (HVS), the most common species was C. Albicans followed by C. tropicalis, C. glabrata, Candida parapsilosis and C. krusei. Among the 16 urine samples, the most common species was C. Albicans followed by C. tropicalis, C. glabrata, and C. krusei were isolated.