Press Residue Research Articles

Optimized microwave-assisted extraction of total phenolic and flavonoid contents from defatted seeds and press residue of Opuntia ficus-indica (l.) mill

Phenolic content of Opuntia ficus indica (OFI) was estimated in samples of seeds and press residue (cake) generated through mechanical oil extraction process. A microwave-assisted extraction (MAE) method was investigated for the optimization of the extracted total phenolic (TPC) and total flavonoid contents (TFC). The influence of extraction parameters on the yields of TPC, TFC were modelled using a second-order regression equation. The optimal MAE conditions were: 61.65 % ethanol concentration, 633.42 W microwave power, 3.59 min irradiation time and 46.28 mL/g solid-to-liquid ratio (SLR) for phenolics extraction, and 54.97 %, 580.04 W, 3.20 min and 47.40 mL/g for flavonoids extraction from defatted seeds powder. The extracts obtained under these conditions showed a TPC and TFC of 416.311±1.154 mg gallic acid equivalent (GAE) per100 g dry weight (DW) and 52.67±0.72 mg quercetin equivalent (QE)/100 g DW, respectively. Regarding press residue, the optimal conditions were 60.26%, 643.89 W, 3.85 min and 47.28 mL/g for phenolics extraction, and 67.96 %, 464.41 W, 2.46 min and 41.04 mL/g for flavonoids extraction; with a TPC and TFC of 400.11±3.14 mg GAE/100 g DW and 38.75±0.16 mg QE/100g DW, respectively. The results of the present study confirmed that OFI seeds and press residues could be considered as a non-negligible source of phenolics with a good antioxidant activity and revealed that MAE is a reliable method for optimizing yields of recovered phytochemicals from such agricultural products derivatives and by-products, with an intent to be scaled-up for industrial, nutraceutical or pharmaceutical applications.

Low-Waste Technology of Fodder Grass Processing

The article describes the peculiar features of plant processing for the conservation of fodder made of the leafy mass of hard-to-ensilage legume family cultures in agricultural complexes and deficiencies of the traditional treatment of the feedstock. The current technology of mechanical fractionation of legume grasses suggests the production of the basic product - the protein concentrate. The by-products are press residues - grass pulp and protein-free liquid, which are production wastes. The purpose of this work was to develop a comprehensive method of plant resources' use with waste disposal and application in fodder production. The novelty of the work is the method of combining the dehydration of the vegetative organs of the hard-to-ensilage sown legume fodder grasses through mechanical pressing and drying and the use of the resulting wastes - the press residues as raw material for fodder preparation. The humidity of the press residues was to be reduced to the level required for making haylage by using the method of low-temperature final drying in an experimental unit - a continuous-action direct heated drier. The results of studying the possibility of reducing the humidity of the press residues by low-temperature direct-heated drying and using it as raw material for haylage are presented in the article. The temperature and time ranges for the material drying in a direct-heated drier were established. The chemical composition of the vegetable stock and resulting haylage was analyzed, and the composition of the fermentation products, the feed, and caloric values, and the potential safety for animal organisms were determined. The suggested method ensures the comprehensive processing of the herbage with waste disposal and the production of high-quality animal fodder. In terms of quality parameters and environmental safety, the obtained fodder conformed to the regulatory requirements for the haylage to be used in the cattle stock diet.

Use of oyster mushrooms (Pleurotus ostreatus) for increased circularity and valorization of rapeseed residues

In Europe, rapeseed is a common oilseed crop, resulting in the production of 20 million tons of rapeseed press cake yearly. This press cake can be further upcycled and a protein fraction can be extracted for food purposes, leaving de-proteinized fiber-rich residues. This study examined the use of these residues in the production of oyster mushrooms (Pleurotus ostreatus) and of the spent substrate as feed, since mushroom cultivation may improve the feed properties of substrate. In terms of mushroom production, the addition of rapeseed press residues was beneficial, giving significantly higher biological efficiency (BE = 93.1 ± 11.0%) compared with the control, sugar beet pulp substrate (70.0 ± 6.6%). This increase in productivity can most likely be explained by higher energy content in the substrate supplemented with lipid-rich rapeseed residues. Despite differences in BE between the substrates, high similarity was observed in lipid composition of the fruiting bodies (lipid profile dominated by linoleic acid (18:2), palmitic acid (16:0), and oleic acid (18:1)), and in protein and moisture content. After mushroom harvest, approximately 70% of the initial dry weight of both substrates remained as a possible feed source. Both substrates had significantly lower levels of carbohydrates and unchanged neutral detergent fiber content after mushroom harvest, and both gave lower in vitro digestibility, total gas production, and methane production. However, protein concentration differed between the substrates, with the highest concentration (15.8% of dry weight) found in spent substrate containing rapeseed press residues. The result of the present study suggests that the de-proteinized rapeseed press residue is a resource well-suited for use in the production of mushrooms and feed.

Reutilization of berry press residues in minced Baltic herring (Clupea harengus membras) – Effect on lipid oxidation and sensory characteristics during cold storage

Lipid oxidation is one of the main causes of quality deterioration in dark-muscled fish. This study investigated the effect of berry side streams of juice pressing or oil extraction at different concentrations on lipid oxidation and sensory quality of minced Baltic herring (Clupea harengus membras) stored at 1 °C. Lipid oxidation was assessed by determining the peroxide value and volatile secondary oxidation products. Generic descriptive analysis and gas chromatography-olfactometry were used to investigate the odor, flavor, and odor-active compounds of minces. Based on the peroxide value and formation of hexanal, 1-penten-3-ol, and 3,5-octadien-2-one, a concentration of 1.5 g/100 g of lingonberry-bilberry juice press residue and sea buckthorn juice press and oil extraction residue was sufficient to retard and reduce the extent of oxidation. Furthermore, the addition of these residues inhibited the formation of fishy odors during 3 days of storage. The control mince stored for 3 days showed the highest number and intensities of oxidation products such as (Z)-4-heptenal, 2,3-pentanedione, and (E,Z)-2,6-nonadienal, while their formation was retarded in the mince containing press residue from sea buckthorn. Berry press residues were efficient in controlling lipid oxidation of minced Baltic herring during storage and have the potential to mask potentially unpleasant fishy odor and flavor.

Turmeric press residue – a high-value by-product of turmeric juice powder

Turmeric press residue is an industrial by-product obtained after the extraction of turmeric juice in theprocess of making turmeric juice powder. In the present study turmeric press residues from four varieties ofturmeric viz. Chintapalli (1-year crop), Chintapalli (2-year crop), IISR-Pragathi and IISR-Prabha were studied forvarious quality parameters. The dry recovery of the press residue varied from 8.07 to 9.70% based on initialmass of fresh turmeric while it varied from 14.52 to 20.61% based on the mass of press residue obtained afterjuice extraction. The moisture content of dry turmeric press residue varied from 7.98 to 8.21%. The essentialoil content of the turmeric press residues for varieties Chintapalli (1-year crop), Chintapalli (2-year crop),IISR-Pragathi and IISR-Prabha was 5.86, 6.30, 5.06 and 5.33%, respectively. The oleoresin content was 9.66,11.92, 10.76 and 15.53%, respectively and the corresponding curcumin content was 3.28, 3.26, 5.13 and 5.21%,respectively. GC-MS analysis revealed that the major volatile oil constituents in the essential oil extracted fromturmeric press fibre were ar-turmerone, turmerone, curlone, β-se squiphellandrene and zingiberene.

Phytochemical characterization of oil and protein fractions isolated from Japanese quince (Chaenomeles japonica) wine by-product

The wine industry generates large quantities of by-products each year. Therefore, this work aimed to isolate and evaluate the oil and protein fractions of Japanese quince (Chaenomeles japonica, JQ) press residue, offering a partial utilization of valuable bioactive compounds of wine industry by-products. To study the JQ oil extract yield, composition and oxidation stability, we modified the co-solvent composition during the supercritical CO2 (SC–CO2) extraction of oil by adding different ethanol content. The remaining defatted material was used for the isolation of proteins. The SC-CO2 extraction yielded oil rich in polyunsaturated fatty acids, tocopherols, and phytosterols. The use of ethanol as a co-solvent increased the oil yield but did not enhance its oxidative stability or content of antioxidants. We recovered protein isolate after removing tannins with 70% ethanol extraction in the next step. The JQ protein isolate contained all essential amino acids. In addition to its balanced amino acid composition, the protein isolate exhibited excellent emulsifying properties highlighting its potential as a food additive. In conclusion, JQ wine by-products can be utilized for the extraction of oil and protein fractions which can be used in food or cosmetic product formulation.

Variation of chemical and sensory profiles of blackcurrant (Ribes nigrum) juices produced from different cultivars of European origins

Blackcurrant cultivars originating from different European countries (Finland, Latvia, Lithuania, Poland, and Scotland) but cultivated at the same geographic location and under the same climatic conditions in Finland were investigated. Berries of 21 cultivars were pressed into juices without enzymatic assistance, and the chemical and sensory profiles were studied. The genotype significantly influenced juice yield (47–70% of berry weight), anthocyanin profile (including 42–64% of delphinidins and 32–54% of cyanidins; rutinosides vs. glucosides, 3–9:1), and sensory properties of the juice (appearance, flavor, and mouthfeel). The studied cultivars exhibited a large diversity in anthocyanin content in berries (904–4595 μg/g of berries), juices (obtained by pressing pulps, 141–1028 μg/g of juices), and press residues (skins and seeds, 3492–19852 μg/g of residues). Variation of anthocyanins in juices was unrelated to the variation in berries. The perceived color was affected by the anthocyanin contents in juices but not by the anthocyanin levels in berries. These measured compounds did not have a direct association with viscosity, flavor, or aftertaste of the juices.

Procyanidins from Cranberry Press Residues-Extraction Optimization, Purification and Characterization.

Procyanidins are a polyphenolic group that can be found in a variety of foods such as chocolate, tea, cranberries and others. Type A procyanidins can be found in a handful of sources and one of the richest sources are American cranberries. These compounds possess antioxidative, anticancer and anti-inflammatory activities and are most widely used as prevention for urinary tract infections. Cranberries are utilized for jam and juice production, and the latter produces industrial food waste press residues. Press residues contain free and bound procyanidins which can be extracted for use as nutraceuticals. In this study, the extraction of cranberry press residues has been optimized using RSM and the resulting extracts have been purified and fractionated. The obtained procyanidin fractions have been investigated for their antioxidative potential and analyzed using LC-ESI-FTICR-HRMS to determine individual procyanidins. The optimization showed that the optimal extraction can be conducted using acetone in a concentration of 53% without the addition of an acidifying agent. Strong correlation was observed for procyanidin contents and their antioxidative activity using DPPH, ABTS and FRAP methods. The purified fractions contained 78 individual (65 Type A) procyanidins with the degree of polymerization of up to 9.

Sustainable production networks: A design methodology based on the cooperation among stakeholders

This paper proposes an approach based on a systemic design methodology and the Multi-layer Enterprise Input-Output formalisation method to engage different stakeholders in a new production network to create value from waste and by-products. The proposed methodology supports the stakeholders' cooperation in the design and development phases to establish, in a bottom-up fashion, production networks based on mutual stakeholders’ trust and relevant innovation content, such as industrial symbioses. The systemic design approach focuses on the characteristics of the local communities and their know-how identifying current waste and potential alternatives to exploit them as raw materials. A novel hierarchical version of the Multi-layer Enterprise Input-Output formalisation method quantitatively supports the system design and the performance assessment. It increases data consistency, supports practitioners in system formalisation, and feeds a properly developed dashboard of KPIs to provide the stakeholders with systemic design information and system techno-economic-environmental performance. The methodology implementation is shown in a case study based on the regional project InnovaEcoFood, which analyses the technical feasibility of producing healthy products from wine press residue and rice bran. The case study shows that the methodology data structure is suitable for dynamic analyses to support decision-making, performance assessment, negotiation, and development activities. The methodology engages stakeholders in the design and development process, providing consistent techno-economic-environmental data through a shared platform. In particular, it is suitable for self-organising networks and models where stakeholders themselves engage partners to build and extend the network.

Impact of enzymatic pre-treatment on composition of nutrients and phytochemicals of canola (Brassica napus) oil press residues

The study aimed to develop a biorefining process to recover proteins and dietary fibres from a food industry side-stream, canola (Brassica napus) oil pressing residues. The materials were treated with commercial protease, carbohydrase, and phytase to obtain protein-rich supernatants and fibre-rich precipitates. The compositions of these fractions were analyzed using LC-MS (glucosinolates and phenolics) and GC–MS (sugars, acids, and amino acids). Compared to raw material, the supernatants were richer in proteins, sugars, acids, amino acids, phenolic acids, and flavonols; the precipitates had higher levels of minerals and dietary fibres. The enzymatic treatment decreased the contents of phytic acid, glucosinolates, and phenolic alkaloids in all fractions. The applied enzymes effectively enhanced solubility of proteins, despite the lower yield of crude proteins compared to the alkaline extraction (40–82 vs 91 g/100 g dry matters). The impact of enzymes on other chemical components was also revealed by using principal component analysis.

Optimisation of blueberry (Vaccinium corymbosum L.) press residue extraction using a combination of pectolytic enzyme and ultrasound treatments

Blueberries are one of the most popular and widely consumed berries, they are usually consumed fresh, dried or processed into juice. During the production of juices large quantities of berry press residues are generated, which are an industrial by-product and they accumulate as food waste. Optimal management of this industrial fruit by-product could help in utilising berry press residues with the purpose to valorise and add economic value. Blueberry press residues consist of skins and seeds which hold valuable compounds with antioxidant activity –polyphenolics. To facilitate the objective of bioactive compound release, the cell walls must rupture. Pectolytic enzymes could be used to degrade the structural polysaccharides of the berry skin cell wall, thus releasing the contents of the cells into the extraction medium. The physical characteristics of berry skins allows the use of pectolytic enzymes for the disruption of cells; however, the effects of enzymatic hydrolysis could be further improved using ultrasound. In this study the combined effects of enzyme and ultrasonic treatments were evaluated and optimised using the Response Surface Methodology approach in order to increase the release of polyphenolic compounds (especially anthocyanins). The optimised method was further tested to evaluate the application potential of enzyme and ultrasound treatment to prepare blueberry or lingonberry juice with increased antioxidant activity and more vibrant colour. The obtained results provide an optional method of berry press residue valorisation to produce higher quality juice or extract bioactive compounds from this type of food-waste.

Quantitation of Mi-saponin A in adulterated Quillaja and contaminated Gypsophila saponin extracts by high performance liquid chromatography/quadrupole time-of-flight mass spectrometry

• Development of a semipreparative HPLC method for the isolation of Mi-saponin A. • New HPLC / Q-ToF method for quantitation of Mi-saponin A in various saponin extracts. • Detection of Mi-saponin A in currently purchased Quillaja and Gypsophila extracts. • Quantitation of Mi-saponin A in these adulterated Quillaja and Gypsophila extracts. Plant saponin extracts are currently of increasing importance for the environment and climate. Saponin extracts are used for the regeneration of soils contaminated by environmental pollutants such as polycyclic aromatic hydrocarbons. Furthermore, as feed additives they may have the potential to reduce the release of the greenhouse gas methane in ruminants, or to reduce ammonia emissions in pigs and broilers. Quillaja extracts play a major role in emission reduction. Some commercially available Quillaja extracts have been adulterated by blending with an inexpensive Madhuca saponins extract. Since a quantitation has not been performed so far, the present work aims at the development of a quantitative method for the determination of the main ingredient Mi-saponin A of Madhuca . For this purpose, a semi-preparative HPLC method for the isolation of Mi-saponin A from mahua seed cake, which is the press residue of the seed kernels of Madhuca longifolia , was developed. Using 1 H NMR, 13 C NMR, and high resolution mass spectrometry, the identity was unambiguously confirmed and the purity was excellent. It was used as standard for quantitation of Mi-saponin A in various currently purchased commercially available Quillaja saponin extracts. The concentration of Mi-saponin A was between 1.68 and 2.65 % (w/w), which reveals that the blending of Quillaja saponin extracts with Madhuca is still relevant. Moreover, the developed method for the quantitation of Mi-saponin A is also suitable for lower concentration. Thus, Mi-saponin A was found at a concentration of 0.10 % (w/w) in Gypsophila saponin extracts, confirming that there was a contamination with this Madhuca saponin.

Discrepancies in the German Pharmacopoeia procedure for quality control of Quillaja saponin extracts.

This study focused on the evaluation of Quillaja saponin extracts with the additional quality designation DAB-which means the abbreviation of the German Pharmacopoeia (Deutsches Arzneibuch). This label suggests that Quillaja saponin extracts marked in this way are of pharmacopoeial quality and thus stand out from other Quillaja saponin extracts. The DAB ninth edition listed Quillaia saponin as a reagent. According to DAB, its quality must be checked by thin-layer chromatography (TLC), and three closely spaced zones in a defined retention factor (Rf) interval specify the saponin reagent. All the Quillaja saponin extracts obtained from different manufacturers and labeled as DAB quality complied with the TLC test. However, the analysis with high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (HPLC-Q-ToF-MS) clearly showed additionally an intense peak pattern of Madhuca saponins in all measured samples. The TLC test for Mahua seed cake, which is the press residue from Madhuca longifolia, surprisingly showed the same three closely spaced zones in the defined Rf interval. The three zones could be identified as Mi-saponins from Madhuca after scraping and extracting them from the stationary phase of the TLC plate and subsequent measurement by HPLC-Q-ToF-MS. Therefore, the specification of the saponin reagent in DAB characterizes erroneously Madhuca saponins that are not listed as a saponin plant source for the saponin reagent.

Steel mill waste application in soil: dynamics of potentially toxic elements in rice and health risk perspectives

Potentially toxic elements (PTEs) are of great concern in steel mill wastes. Therefore, in order to use them as potential fertilizers in soil, risk assessments are needed. Three steel mill wastes were tested as possible amendments for soils at seven different doses (0, 0.5, 1, 2, 4, 8, 16 t ha-1): phosphate mud (PM), metallurgical press residue (MPR), and filter press mud (FPM) during rice cultivation in a pot experiment in a Haplic Gleisol. Analysis on rice tissues, including roots, shoots, husk, and grains, were conducted and contents of Cu, Cd, Ni, Zn, Mn, and Pb were assessed. Translocation and bioaccumulation factors were calculated for each element. In general, PTEs are more accumulated in roots and greater contents of Zn and Mn were found, while the lowest ones were found for Pb, probably due to its lack of functional roles during plants development. Higher translocation was observed for Mn, which is associated to the redox conditions of rice cultivation and the high mobility of this element under this condition. Application of steel mill wastes can increase PTE bioavailability and translocation factors, especially PM, but all of the wastes reveal a high hazard index.

Optimization of extraction time and temperature for natural antioxidants of öküzgözü grape pomace using various solvent ratios

Abstract Grape pomace, a press residue of winemaking process, has great usage potential in many fields because of its phenolic components such as anthocyanins, antioxidants and dietary fiber. In this study, it was aimed to determine most suitable parameters for production of food colorant from grape pomace (variety of okuzgozu) under different extraction conditions, thus utilising waste of wine production. The dried grape pomace was extracted at 3 various temperatures, 4 different periods and 5 different solvent ratios. 12 different anthocyanins, the free radical scavenging activity, total phenolic content and total monomeric anthocyanin of the extracts were determined and values of the samples ranged between 5.2-676.1 (mg/kg), 1.99-3.65 (IC50 mg/mL), 83.68-1598.57 (mg gallic acid/100 g) and 730.7-1850.3 (mg/100 g as mv-3-glc equivalents), respectively. The most suitable temperature, time and solvent ratio for the highest extraction of anthocyanins were obtained at 50 °C and 50:50, 180 min, respectively.

Composition of biologically active substances of Vaccinium berries growing in the northwestern region of Russia

Using gas chromatography with preliminary derivatization of samples, the composition of the biologically active substances of wild-growing cranberries (Vaccinium oxycoccos L.) and lingonberries (Vaccinium vitis idaea L.) was studied. Biologically active substances contain phenolic compounds, tocopherols and phytosterols. Flavonoids prevail in the composition of phenolic compounds, the amount of which increases in berry press residues. Cranberries and lingonberries differ in the quantitative and qualitative composition of flavonoids and phenolic acids. In fresh cranberry berries, taxifolin and morin predominate among flavonoids; catechin and taxifolin predominate in lingonberries. In berry press residues, the number of flavonoids increases due to taxifolin and qercetin B, and in lingonberries, additionally due to catechin. Chlorogenic acid predominates in the composition of phenolic acids of fresh cranberries; 4-Oxybenzoic acid predominates in fresh lingonberries. Tocopherols quantitatively prevail in whole lingonberries, and phytosterols predominate in cranberries. In the berry press residues, their number increases.

Steel mill waste effects on rice growth: comparison of chemical extractants on lead and zinc availability.

Zinc deficiency is widespread in cultivated soils, limiting the grain crop production and the adequate human nutrition. Several wastes from metallurgical activity can be used as Zn source, but these materials generally also have other potentially toxic elements, such as Pb, that can be highly toxic for plants and humans. This study aimed to evaluate the efficiency of five chemical extractors (water, citric acid, DTPA, Mehlich 1, and USEPA 3051A) in better correlating with the bioavailable contents of Zn and Pb in soils treated with steel mill wastes (metallurgic press residue (MPR), filter press mud (FPM), and phosphate mud (PM)). Rice plants were cultivated in pots with 4 kg of a Haplic Eutrophic Gleisol and steel mill wastes were applied in soil at increasing doses (0, 0.5, 1, 2, 4, 8, and 16 t ha-1). The availability of the potentially toxic elements Zn and Pb was assessed as total contents in rice shoots, grains, husks, and roots. The results showed that the USEPA 3051A method extracted greater contents of Zn and Pb from soil compared with other extractants. Due to their greater natural Pb and Zn contents, MPR and PM promoted higher contents of these elements in soils, respectively. Doses of PM influenced Zn contents in grains. After adding 16 t ha-1 of PM, Zn content in rice grains was 0.1 mg kg-1. However, at doses 0.5, 1, 2, and 4 t ha-1, the average concentration of Zn in the grains was 40 mg kg-1. The wastes MPR and FPM at 16 t ha-1 promoted Zn concentration in grains of 42 and 45 mg kg-1, respectively. The greatest contents of Pb in grains were found after addition of FPM at doses 0.5, 1, and 2 t ha-1: 6.67, 4.96, and 0.45 mg kg-1, respectively, and above 4 t ha-1 (4, 8, and 16 t ha-1); Pb content in grains was less than 0.3 mg kg-1. The content of Pb in roots at 16 t ha-1 of PM, MPR, and FPM was 18, 25, and 155 mg kg-1, respectively, and for Zn, under the same conditions, 100, 255, and 813 mg kg-1 for MPR, FPM, and PM, respectively. USEPA 3051A can be used to assess Pb and Zn available contents, and positive correlations with bioavailable contents of these elements in roots prove its feasibility. Further studies are necessary to state the safety of using steel mill application, including the use of other crop species, but PM is a promising waste for soil Zn fertilization.

Implementation of a differentiated approach to the use of microwaves processing in the production of gelatin jellies

The market for jelly products based on natural raw materials is promising and is constantly expanding. For the production of gelatin jellies based on extracts from berry press residues, microwave technology was used, which made it possible to exclude the operation of swelling of gelatin for renaturation of proteins. The differentiated approach to the use of microwaves in the production of gelatin jellies influenced the technological parameters (time of heating the recipe mixture in a microwave oven; time of formation of the gelatin jellies structure upon cooling) and quality characteristics (plasticity, antioxidant activity). The absence of the gelatin swelling operation increased the microwave processing by 30-50% and the gelatin jellies structure formation time increased by 20-30%, depending on the volume of the recipe mixture for processing and the weight of the finished product. Microwave processing increased the plasticity and antioxidant activity of gelatin jellies, which depended on the type of berry press residues used for extracts. The higher the antioxidant activity values (DPPH & FRAP) of gelatin jellies were, the more their deformation values decreased. Gelatin jellies based on extract from blueberry press residues had the highest antioxidant activity and the lowest deformation values, while retaining their shape.

Development of energy-saving methods of waste processing as a tool for transformation of the social and economic systems

The article deals with the problems of preservation of leafy mass of legume family high-protein agricultural crops, the disadvantages of the crop high-temperature drying in fodder production. One presents the results of studying the possibility of reducing the moisture content in plants mechanically, using the resulting waste (press residues) as a raw material for silage preparation applying the energy-saving bioconversion method. The results of developing the method of alfalfa press residues ensilaging with introduction of a mixture of strains of lactic acid bacteria cultures are presented The chemical composition of the vegetable stock and resulting silage was analysed, the feed and caloric values were determined for introduction into animal diets.

Waste processing method and environmental protection in the agro-industrial complex

The article presents the features of conservation of leafy mass of high-protein crops of the legume family, the disadvantages of traditional preparation of biomass for silage in fodder production. The results of studying the possibility of reducing plant moisture by mechanical means, using the press residue as a raw material for making haylage, and brown juice as a preservative are presented. The results of the development of a method for silage of the pressed alfalfa residue with the introduction of fermented brown juice enriched with the metabolic products of lactic acid and propionic acid bacteria - organic acids, to intensify the process of fermentation of feed. The analysis of the chemical composition of plant raw materials and the obtained haylage was carried out, the value of fodder, energy value and potential harmlessness for feeding to animals was determined.