Pre-sowing Treatment Of Seeds Research Articles

Biological effect of dual-frequency laser pulse radiation during pre-sowing treatment of rice seeds at early stages of development under stressful environmental conditions

The biological effect of two-frequency laser pulsed radiation produced on copper vapor during pre-sowing treatment of rice seeds of Veles and Leader varieties at early stages of development in extreme stressful environmental conditions has been studied. The copper vapor laser used in the experiment had the following output characteristics: wavelengths of 510.6 nm (green radiation line) and 578.2 nm (yellow radiation line), pulse duration of 15 ns, repetition frequency of 10 kHz, total pulse power of 10 kW, energy-power ratio between the green and yellow radiation lines 3/1 The interaction of laser radiation with wavelengths of 510.6 and 578.2 nm in the nonlinear medium of the seed caused the formation of additional wavelengths: a total wavelength of 271 nm (ultraviolet radiation) and a difference wavelength of 4.37 microns. Exposure to each of the four wavelengths in its spectral range could lead to the initiation of at least four radiation-induced biochemical reactions. It is shown that pre-sowing treatment of rice seeds with two-frequency laser pulsed radiation for 5–20 seconds had a stimulating effect on the growth and development of rice (the greatest effect was when exposed to 5–10 seconds), and also increased its stability when grown in extreme stressful environmental conditions (moisture deficiency, depleted soil) at early stages of development.

Usage of an intense electromagnetic field for pre-sowing treatment of oil radish seeds

Usage of an intense electromagnetic field for pre-sowing treatment of oil radish seeds

Sowing properties of sunflower seeds of Talento hybrid under the influence of a modified plant growth regulator

The improvement of agricultural crop cultivation technologies enables the production of high sunflower yields with high-quality indicators. One such element is pre-sowing seed treatment. This study aimed to investigate the effect of a plant growth regulator supplemented with calcium on the sowing properties of sunflower seeds. The experiment was conducted under laboratory conditions. For pre-sowing seed treatment, a semi-synthetic film-forming agent with an anti-stress effect was used, which was modified with Ca2+ ions in the form of calcium chloride. It was established that the encrustation of sunflower seed with a growth regulator stimulates germination processes and activates the initial stages of sunflower organogenesis. This increases the indicators of germinative vigour and germination capacity. Adding calcium to the plant growth regulator contributes to a further increase in the indicators of germinative vigour and seed germination capacity of sunflowers by 5.4% and 7.7%, respectively, compared to the control. At the same time, the hypocotyl length increased by 1.51 times compared to the control, while the root length increased by 1.37 times. The accumulation of organic substances in sunflower seedlings is characterised by an increase in dry matter. When calcium was added to the plant growth regulator, a more intensive accumulation of dry matter in the hypocotyl and root was observed compared to the control and the variant using only the growth regulator. The establishment of a ranking range indicated that, based on the parameters studied, the optimal treatment option for the pre-sowing treatment of sunflower seeds is the application of a plant growth regulator containing calcium ions at a concentration of 1.0 g/L. The use of this preparation is relevant and promising for studying the formation of sunflower plant productivity with increased quality indicators under field conditions

Efficiency of pre-sowing treatment of white lupine seeds with complex compositions based on Tebu 60 fungicide

The results of laboratory and field tests of effectiveness of the Tebu 60 protectant, ME (microemulsion) in its pure form and in tank mixtures with Humat+7 and Fitaktiv Vita against anthracnose seed infection and other diseases of lupine are presented. The research was conducted in 2020–2022 in the Bryansk region. The object of the study were the seeds, seedlings and crops of the Michurinsky white lupine variety. In laboratory conditions, biological evaluation of the effectiveness of the Tebu 60 protectant in pure form (rate of consumption – 0.5 l/t) and tank mixtures Tebu 60 + Humate+7 (0.5 + 0.5 l/t) and Tebu 60 + Fitaktiv Vita (0.5 + 0.05 l/t) against anthracnose seed infection was carried out by the number of affected seedlings grown in paper-polyethylene rolls in comparison with the control (without treatment). The tank mixture Tebu 60 + Fitaktiv Vita (100%) showed high biological efficacy against anthracnose. Moreover, this mixture significantly (LSD05 = 0.66) had the least effect on reducing the hypocotyl length of the seedlings (8.8%). The field experiment was laid down in a fourfold repetition. The plot area was 34 m2. The seeding rate was 1.0 million germinated seeds/ha. The soil of the site was gray forest soil, humus content – 2.6%. The forecrop was spring cereal crops. Seed dressing with Tebu 60 (0.5 l/t) and tank mixtures Tebu 60 + Humate+7 (0.5 + 0.5 l/t) and Tebu 60 + Fitaktiv Vita (0.5 + 0.05 l/t) were conducted one month before the sowing. The effectiveness of the treatment was evaluated in comparison with the control. On average, the highest biological efficacy against anthracnose seed infection was shown by the tank mixture Tebu 60 + Fitaktiv Vita (98.1%). By the shiny pod phase, the number of affected beans was 20.5% compared to 78.4% in the control. The biological efficacy against fusarium disease and rhizoctonia was 65.9 and 74.4%, respectively. The prevalence of gray rot and white rot on beans was reduced 8 and 11 times, respectively. Seed germination increased significantly (LSD05 = 0.74) by 16.2%, safety of productive plants to harvesting – by 56.5%. Reliable increase in the seed yield was 2.52 t/ha, cost recovery – 64.88 rubles.

THE INFLUENCE OF MULTICOMPONENT WINTER WHEAT SEED PROTECTANTS ON ITS PRODUCTIVITY AND QUALITY

We studied combined suspension compositions prepared by liquid-phase mechanical treatment based on the fungicides difenoconazole, tebuconazole, imazalil, fludioxanil, thiabendazole, prochloraz, sedoxan and the insecticides thiamethoxam, imidacloprid, plant growth regulators Enershanse, which showed a wide range of biological activity in field conditions. It was established that the main indicators of the quality of the final product - the nature of the grain, the amount of protein and gluten, glassiness, and the quality of gluten - depended on the fungicidal and insecticidal effects on the phytosanitary condition of winter wheat crops. All studied drugs had a protective effect. They contributed to the disinfection of winter wheat seeds from seed and soil pathology and harmful insects of the early period of plant development. It was found that for effective treatment of winter wheat seeds, it is best to use insectofungicidal preparations containing several components of different chemical classes. It was found that pre-sowing treatment of winter wheat seeds with a suspension composition of thiamethoxam + sedaxan + fludioxonil + tebuconazole in the form of Vibranse Integral, suspension concentrate provided the greatest increase in grain yield by 0.49 t/ha (7.3%) with a control yield of 6.74 t/ha. The use of this drug ensures a particularly high protein content in the grain (16.07%) and gluten (36.8%) of the first quality group. We have established diverse positive effects of the drug Vibranse Integral, manifested in enhancing the growth processes of the root system due to the presence of the sedaxane molecule, the purpose of which is aimed at the formation of a healthy and well-developed root system, increasing the stress resistance of plants to abiotic factors, and the manifestation of a good protective effect in relation to soil and seed infections. We determined the lowest, relative to other studied drugs, yield (6.17 t/ha) from the use of a suspension composition of thiamethoxam (262.5 g/l) + difenoconazole (25 g/l) + fludioxonil (25 g/l (Celest Top), but the grain was of high quality. All other seed protectants occupied an intermediate position between the above-mentioned drugs.

Перспективы переработки угля с низкой степенью метаморфизма

Introduction. The relevance and significance of this work is due to the fact that the soils of Kazakhstan are about 48% salt marshes. The reasons for their occurrence are different: harsh climatic conditions, the drying Aral Sea, the Baikonur cosmodrome, numerous test sites, etc. With an increased content of water-soluble salts in the root layer of soils, most cultivated plants develop poorly and yield a reduced yield. To solve the above problem, the technology of processing brown coal from the Kiyakty deposit and its substandard part for the production of drugs-growth stimulants for agricultural plants, high-calorie fuel briquettes and coal-alkali reagents is proposed. Materials and methods. Development of technology for the production of a humic preparation from brown coal deposits in Kazakhstan. It is known that these coals are amorphous polymers containing bitumen, humic acids, residual organic substances and moisture. Laboratory experiments were carried out by the method of biotesting according to B.P. Strogonov in glasses with a capacity of 0.5 liters in accordance with the requirements of GOST (GOST 10250–80, GOST 12038–84). The methodology for conducting research on the manufacture of briquettes, carbon-alkali reagents based on the humic acid hypothesis and various local binding materials with the involvement of certified CeLSIM laboratories is substantiated. The most optimal binder is a liquid bardic concentrate. Results. As a result of the application of new methods of pre-sowing treatment of rice seeds with a 2.5 % aqueous solution of the preparation from coal, its yield increased by 9.5–12.1 quintals per 1 ha, or by 33.8–41 % compared with the control. Discussion. The works of a number of foreign and domestic researchers on this topic have proved the possibility of using humic substances from brown coals to obtain organo-mineral fertilizers, and a comparative study of the chemical composition has been conducted. However, according to our technology, it is clear that we do not get fertilizer, but plant growth stimulants. It should be noted that the technology developed by the authors makes it possible to obtain a humic preparation, the use of which on low-yielding soils of Kazakhstan ensures a stable increase in the yield of cereals and legumes. Conclusion. The developed technology for the production of humic preparations for various purposes can be used to increase crop yields on degraded soils, for the bio recultivation of man-made formations, the manufacture of a substrate for greenhouse farms, the production of fuel briquettes and coal-alkali reagents, as well as during the operation of other brown coal deposits of the Republic of Kazakhstan with a high content of humic acids in their composition. Suggestions for practical applications and directions for future research. The territory of Kazakhstan is considered to be arid zones of dry steppe soils without rains. In these conditions, agricultural technicians resort to plants of halophyte varieties, therefore, the prospects for using this technology are very large, for example, to suppress dust formation on the surface of tailings ponds with the cultivation of green mass of wild plant varieties. Preparations from brown coals can be used to purify water and wines, leach precious metals, etc.

Application of vermiculture products as an innovative direction of organic production

An important strategy for diversifying agricultural production is the use of vermicomposting and the production of new products from vermicompostfor their further widespread use. Objective. To investigate the effectiveness of the use of vermicompost and the biological product ‘Nanoverm’ obtained from it as a high-quality complex environmentally friendly organic fertiliser for application to grain crops. Methods. The research was conducted at LIGA COLAP LLC, the Department of General and Applied Ecology and Zoology of Zaporizhzhia National University (Zaporizhzhia) and farms of the Vilnyansky and Orikhivsky districts of Zaporizhzhia region in two stages. At the first stage, the effect of vermicompost at a dose of 1.0-1.5 l/t and Nanoverm at a dose of 1.0-1.5 l/t on the first stages of winter wheat ontogeny of Podolyanka variety was studied. At the second stage, the effect of the biological product ‘Nanoverm’ on the formation of biometric growth parameters and yield of winter wheat was studied. Plants of the control group were grown without fertilizers, and the experimental group was treated with Nanoverm biofertilizer at a dose of 1.5 l/t. Results. It was found that pre-sowing treatment of winter wheat seeds with vermicompost provided an increase in seed germination energy by 3.7-4.0 % compared to the control, and laboratory germination by 5.3 %. When seeds were treated with Nanoverm biological product at a dose of 1 l/t, germination energy increased by 4.7 %, and laboratory germination increased by 6.0 %. With an increase in the dose of the preparation to 1.5 l/t, these indicators increased by 5.0 and 7.3 %, respectively. Pre-sowing treatment of seeds with the studied preparations also had a positive effect on such indicators as the length of the coleoptile and roots of winter wheat seedlings. The analysis of biometric parameters of winter wheat growth and yield after harvesting showed that in the experimental variant, such indicators as grain weight, number of grains per ear and yield were significantly higher. With almost the same plant height and a slight increase in the length of the ear, the stimulating effect of feeding winter wheat in the earing phase with Nanoverm was expressed in an increase in the number of grains per ear by 15.3 %, and the weight of grain per ear by 22.2 % (p<0.001). As a result, the grain yield in the experimental group was 8.3 c/ha higher than in the control group (p<0.001). Conclusions. It was found that pre-sowing treatment of seeds with vermigum or biological product ‘Nanoverm ’ improves all the indicators that characterise the first stages of ontogeny of winter wheat variety Podolyanka. Fertilising winter wheat in the earing phase with Nanoverm at a dose of 1.5 l/ha led to the activation ofgrowth processes and contributed to the formation of higher productivity. As a result of a significant increase in the number and weight of grains in the ear, the yield increased by 22.3 % and amounted to 47.2 c/ha. Keywords: california worms, vermicompost, Nanoverm, winter wheat, Podolianka, growth, yield.

The influence of pre-sowing treatment of seeds with molybdenum on the yield of pea grain in the conditions of the Republic of Bashkortostan

Relevance. Molybdenum is an essential micronutrient element necessary for plant growth and development. In addition to the fact that molybdenum is part of hormones and enzymes, in legumes it improves nitrogen fixation and nitrogen nutrition, increases the efficiency of phosphorus-potassium fertilizers, thereby increasing productivity.The purpose of this work is to assess the effect of pre–sowing seed treatment with various doses of molybdenum on the yield of pea grain.The experiments were carried out in 2019–2023 in the conditions of the Cis-Ural steppe of the Republic of Bashkortostan. The material for the study was the pea cultivar Pamyati Popova.Methods. An ammonium molybdate solution at a dose of 10, 25, 40, 55 g of molybdenum per 1 kg of seeds was used for pre-sowing treatment. Phenological observations, determination of the completeness of germination and plant height, analysis of the yield structure were carried out in accordance with the methodology of the state cultivar testing of agricultural crops.Results. It was found that pre-sowing treatment of pea seeds of the cultivar Pamyati Popova with molybdenum at a dose of 40 and 55 g/c compared to the control resulted in a reliable (p ≤ 0.05) increase in field germination, safety of plants for harvesting, the number of nodules on 10 plants and their weight. As a result of processing pea seeds with molybdenum at doses of 25, 40 and 55 g/c, the number of beans per plant increased by 5.7–11.4%, the number of seeds per plant – by 13.2–19.5%, grain yield – by 7.3–10.7% compared with the control. Thus, in our experiments, pre-sowing treatment of seeds with molybdenum at a dose of 25–40 g/c was optimal for increasing the value of pea grain yield.

Nanosecond-pulsed plasma protects against bacterial fruit blotch and promotes melon seedling growth.

Bacterial fruit blotch (BFB), known as the 'cancer' of cucurbits, is a seed-borne disease of melons caused by Acidovorax citrulli. Traditional chemical treatments for BFB are ineffective and adversely affect the environment. Using dielectric barrier discharge (DBD) nanosecond-pulsed plasma technology, melon seeds were treated to promote germination and growth and to control BFB. Based on the evaluation parameters of seed germination, seedling growth, leaf yellowing and bacterial infection after seed plasma treatments, 9 min at 20 kV was selected as the optimal plasma discharge parameter. In this study, seedling growth was significantly improved after treating melon seeds carrying A. citrulli using this discharge parameter. The number of first true leaves measured on the eighth day was 2.3 times higher and the disease index was reduced by 60.5% compared to the control group. Attenuated total reflectance-Fourier transform infrared measurements show that plasma treatments penetrate the seed coat and denature polysaccharides and proteins in the seed kernel, affecting their growth and sterilization properties. Pre-sowing treatment of melon seeds carrying A. citrulli using nanosecond-pulsed plasma technology can effectively control seedling BFB disease and promote melon seedling growth by optimizing DBD parameters. © 2024 Society of Chemical Industry.

ПРОРОСТАННЯ НАСІННЯ ПШЕНИЦІ ОЗИМОЇ ЗАЛЕЖНО ВІД ПЕРЕДПОСІВНОЇ ОБРОБКИ РЕГУЛЯТОРАМИ РОСТУ

Modern technologies for growing grain crops require the use of environmentally safe measures and methods. The pre-sowing treatment of wheat seeds necessitates the correct choice of drugs with a wide spectrum of influence on the morphological and physiological indicators of seedlings. From 2019 to 2022, the effectiveness of pre-sowing treatment of winter wheat seeds was studied using the growth regulators 1-naphthyl-acetic acid (25 ml/l), succinic acid (0.025 % solution), and the organo-mineral preparation Fulvohumin (1 % solution) both separately and in combination: 1-naphthyl-acetic acid + Fulvohumin, 1-naphthyl-acetic acid + succinic acid, succinic acid + Fulvohumin, and 1-naphthyl-acetic acid + succinic acid + Fulvohumin. The working solution consumption was 10 liters per ton of seeds. Treated winter wheat seeds were germinated for eight days at a temperature of +20°C. Laboratory seed germination, biometric parameters, and dry and wet weight of seedlings were determined and statistically analyzed. It was established that succinic acid significantly increases seed germination by 11 %, but seedlings treated with succinic acid showed slightly smaller seedling length and raw weight than the control. Pre-sowing treatment of winter wheat seeds with Fulvohumin significantly increases laboratory seed germination (up to 95 %), root length, and total seedling length. This treatment resulted in a 34.5 % increase in the dry weight of seedlings compared to the control. The highest content of dry matter was observed after seed treatment with a mixture of 1-naphthyl-acetic acid + Fulvohumin + succinic acid, reaching 17.4 %. The combination treatment with Fulvohumin + succinic acid produced the tallest seedlings (14.12 cm) and the greatest raw weight (131.39 mg). A significant increase in raw and dry mass of 8-day-old seedlings was noted after seed treatment with the mixture of 1-naphthyl-acetic acid + succinic acid. However, pre-sowing treatment of winter wheat seeds with only 1-naphthyl-acetic acid (0.025 % solution) was not effective, as it did not significantly increase germination (only by 1.3 %). Additionally, seedlings treated with 1-naphthyl-acetic acid had the shortest root length (4.99–5.78 cm). Therefore, the use of Fulvohumin enhances the effectiveness of growth regulators such as succinic acid and 1-naphthyl-acetic acid.

ВПЛИВ МЕТАБОЛІЧНО АКТИВНИХ РЕЧОВИН ТА ЇХ КОМБІНАЦІЙ НА АСИМІЛЯЦІЙНІ ПРОЦЕСИ ПРОРОСТКІВ GINKGO BILOBA L.

Ginkgo biloba is a valuable ornamental gymnosperm plant. To ensure an adequate supply of planting material for this species, measures must be taken to cultivate high-quality seedlings resistant to adverse conditions. Pre-sowing treatment of seeds with metabolically active substances can serve as such a measure. The aim of this study was to investigate the growth processes, leaf apparatus formation, and photosynthetic activity of young Ginkgo biloba plants under the influence of metabolically active substances and their combinations. Metabolically active substances used to treat ginkgo seeds included vitamin E, ubiquinone-10, paraoxybenzoic acid (POBA), magnesium sulfate (MgSO4), and methionine. Their combinations were also tested: vitamin E + ubiquinone-10, vitamin E + POBA + methionine, and vitamin E + POBA + methionine + MgSO4. The growth biostimulator Stimpo was used for comparison. It was established that pre-sowing treatment with these metabolically active substances and their combinations contributed to the formation of a more robust photosynthetic apparatus in ginkgo seedlings. MgSO4 and the combination of vitamin E + methionine + POBA + MgSO4 had the most significant effect on stem height. Among single-component substances, ubiquinone-10 and the combination of vitamin E + methionine + POBA + MgSO4 most effectively increased the average number of leaves. All studied combinations of compounds positively impacted the average leaf area, increasing it by 44.5-58% compared to the control. Among single-component substances, vitamin E and MgSO4 were particularly effective in increasing leaf area. Ubiquinone-10 and the combination of vitamin E + methionine + POBA significantly enhanced the content of chlorophyll a and b pigments in the leaves. The use of metabolically active substances and their combinations during pre-sowing seed treatment is an effective measure to improve the assimilation processes and overall growth of Ginkgo biloba seedlings.

Impact of biological fungicides on the formation and functioning of symbiotic system soybean–Bradyrhizobium japonicum

Background. In order to reduce the negative impact of mineral fertilizers and pesticides on agroecosystems, environmentally friendly plant protection systems are increasingly being utilized in soybean cultivation technologies. These systems aim to provide essential nutrients to plants while minimizing ecological harm. Materials and Methods. In vegetative studies, the processes of formation and functioning of symbiotic systems in soybeans with active strains of nodulating bacteria Bradyrhizobium japonicum (РC07, PC09, B78, B144) were investigated under the influence of pre-sowing seed treatment with the biofungicides Mycosan-N and Phytocide-r. Microbiological, physiological, statistical methods, and gas chromatography were employed in the research. Results. The impact of biological fungicides on the nodulation activity of nodule bacteria has been identified. It was determined that with comprehensive treatment of soybean seeds with B. japonicum РC07 and Mycosan-N, the mass of nodules formed on the roots exceeded that of the control plants by 7‒22 %, and under the influence of Phytocide-r, the mass was lower by 6‒20 % throughout the vegetation period. With the combined application of B. japonicum PC09 and Mycosan-N, a decrease in the number of root nodules by 8‒25 % and a reduction in their mass by 18‒35 % was observed throughout the vegetation period. Under the influence of Phytocide-r, with the inoculation of rhizobia strain PC09, the number of nodules was lower compared to the control plants only in the full flowering stage. At the same time, the reduction in their mass was 6‒20 % throughout the entire observation period. A stimulating effect of both biofungicides on the formation of the symbiotic apparatus involving strains B. japonicum B78 and B144 has been identified. With the combined application of B. japonicum РC07 and Phytocide-r, nitrogenase activity of symbiotic systems decreased by 7 % only in the stage of three true leaves compared to plants whose seeds were treated only with rhizobia. The nitrogenase activity of symbiotic systems formed with the participation of B. japonicum B78 and under the influence of Mycosan-N increased by 28 %, 15 %, and 12 % in the stages of three true leaves, budding, and full flowering, respectively. The action of Phytocide-r, with the inoculation by the nodule bacteria B78, resulted in an increase in nitrogenase activity by 14 % only in the budding stage. Under the comprehensive treatment of soybean seeds with involved biofungicides and B. japonicum B144, an increase in the intensity of N2 assimilation was noted by 29‒34 % in the stage of three true leaves and by 10‒16 % in the budding stage. Conclusions. The application of a scientifically justified selection of rhizobial strains and biological plant protection agents for pre-sowing treatment of soybean seeds will enable a more complete realization of their nitrogen-fixing potential and phytoprotective effects.

The effectiveness of using a plant growth stimulator based on Ambrosia artemisiifolia when growing corn in the Kabardino-Balkarian Republic

The article presents the results of studies on the use of a biological product based on ragweed, due to its chemical composition, on corn crops. Double treatment of corn crops with a plant growth stimulator based on ragweed (Ambrosia artemisiifolia L.) was carried out at growth phases of 3–5 and 6–7 leaves in calculated doses with a working solution consumption of 300 l/ha. The repetition in the experiments is 4-fold, the placement of the variants of the experiment is randomized. Plot area – 50 sq.m. The use of a plant growth stimulator based on ragweed as a pre-sowing treatment of seeds and foliar dressings can increase the yield of corn grain by 14–40%, depending on the corn hybrids studied in the experiment. On the variants of the experiment, where the combined use of organic fertilizer Biohumus and plant growth stimulator based on ragweed (solution 3) was studied, the above quality indicators were higher than the values of the control variant by 1.6; 6.0 and 1.5% – in the hybrid Ladoga 191 MV; at 3.2; 3.0 and 3.8% – Krasnodar 196 MV; by 2.9; 5.9 and 2.2% – Darina MV; at 3.3; 6.2 and 3.7% – Kuban. In the variant of the experiment with the joint use of organic fertilizer Biohumus and plant growth stimulator based on ragweed (solution 3), the value of the conditional income indicator decreased by 5400 rubles, according to the variant of the experiment with the joint use of organic fertilizer Biohumus and stimulant growth of plants based on ragweed (solution 4), the value of this indicator decreased by 7200 rubles, relative to the variant of the experiment with the combined use of organic fertilizer Biohumus and plant growth stimulator based on ragweed (solution 1) .

Восстановление плодородия малопродуктивных дерново-подзолистых почв, вновь вводимых в сельскохозяйственный оборот

The purpose of research is to develop agro-reclamation measures to restore the fertility of low-productive sod-podzolic sandy loam soils for the conditions of the southern part of the Non-Chernozem Zone of Russia. Objectives: to conduct a comparative assessment of the impact of granular fertilizer based on turkey droppings and a liquid-phase biological product (LPBP) on increasing soil fertility, phenological characteristics of plants, crop yields and their quality. Six variants of the field experiment were developed: control variant (without fertilizers); granular fertilizer based on turkey droppings at a dose of 15 t/ha; granular fertilizer based on turkey manure at a dose of 15 t/ha together with pre-sowing seed treatment with 1 % LPBP; pre-sowing seed treatment with 1 % LPBP; granular fertilizer based on turkey droppings at a dose of 30 t/ha. The application of organic ameliorant and pre-sowing treatment of seeds with a biological pre¬paration help to improve the agrochemical parameters of the soil, and, as a result, a higher yield of perennial grasses. The best option is option G30, where the yield was 48.3 t/ha of green mass of grass in total for 2 cuttings, which is 33.8 % more than the control option. In the G15 and G15 LPBP options, the harvest was practically not inferior and exceeded the control option by 30.5 and 24.4 %, respectively, soil fertility indicators improved: soil acidity decreased to 4.8–5.0 (pHsol) and 5.4–5.6 (pHwod), an increase in organic matter by 33.9 %, the content of mobile phosphorus and mobile potassium, and the concentration of total nitrogen increased significantly.

Improvement of the Morphophysiological Parameters of Pepper after the Presowing Treatment of Seeds with Zinc Nanoparticles

Improvement of the Morphophysiological Parameters of Pepper after the Presowing Treatment of Seeds with Zinc Nanoparticles

Seed pre-treatment with electromagnetic field (EMF) differentially enhances germination kinetics and seedling growth of maize (Zea mays L.)

Pre-sowing treatment of seeds with different intensities and exposure times to electromagnetic fields (EMF) has proved to be beneficial in increasing the productivity of crops. The purpose of the recent study was to evaluate the effects of electromagnetic fields applied prior to cultivation to seeds of two maize genotypes namely FH-1046 and YH-5427. Sterilized seeds were exposed to three doses of EMF and sown in six treatments levels i.e. T0 (as control), T1 (60mT for 3 min.), T2 (120mT for 3 min.), T3 (180mT for 3 min.), T4 (60mT for 6 min.), T5 (120mT for 6 min.), and T6 (180mT for 6 min.). There were three replicates in each treatment. Results showed EMF exposure as an augmenting factor as it caused up to 50 % improvement in seed germination performance. The EMF exposure increased germination kinetics and triggered enhancement in morphological characteristics i.e., higher leaf area, plant height, more fresh and dry weight; higher concentration of physiological pigments like chlorophyll a, b, and carotenoids; upregulated antioxidative enzymatic activities; and, higher mineral ions concentration such as Na+, K+, and Ca2+. A growth promotory effect of all doses of electromagnetic field was observed on crop performance, however, T3 (180mT for 3 min.) and T5 (120mT for 6 min.) resulted in the highest germination indices, vegetative proliferations and biochemical assays. Of the two genotypes of maize, FH-1046 proved to be more sensitive the EMF as compared to YH-5427. It was concluded that maize growth and grain yield might be directly enhanced through the useful aspects of EMF by its analytical and sustainable utilization at the large scale of agricultural farming.

Effectiveness of Rhodococcus erythropolis strain OPI-01 on the fungal development in winter wheat

Abstract. Behzad A, Diakite S, Astarkhanova TS, Pakina EN, Allen D, Mohammadi PM, Diarra O, Saquee FS. 2024. Effectiveness of Rhodococcus erythropolis strain OPI-01 on the fungal development in winter wheat. Biodiversitas 25: 1063-1070. One of the means of sustainable wheat production is using bacterial pesticides, which can synthesize phytohormonal substances with antibacterial and antifungal action. Therefore, the work aimed to study the effect of different doses of a new growth regulator Restart, ZH (Rhodococcus erythropolis D strain OPI-01) on winter wheat yield. The findings of field experiments on the effect of plant growth regulator R. erythropolis strain OPI-01 method of pre-sowing treatment of seeds (at 0.1 L/t and 0.2 L/t), and soil spraying just before sowing (-0.5 l/ha) revealed that on average, during the 2020-2021 and 2021-2022 seasons, the number of productive stems increased by a margin of 23-30, the number of grains in 1 ear increased in the experimental units by 16.2-19.4%, and the length of the ear by 0.3-0.7cm, gluten varied from 32.3 to 33.8%, yield increased from 27.8-33.0% relative to the control. Disease severity and incidence of powdery mildew, brown rust and Septoria disease in plots amended withR. erythropolis strain OPI-01 was 2-fold less than in the control plots. Research trials show the efficacy of R. erythropolis strain OPI-01 under field conditions as a plant growth regulator and a potential microbial fungicide against some foliar diseases of winter wheat. It would be exciting to study the mechanism of R. erythropolis on wheat pathogens in vitro and in planta which are still less developed in the literature.

Morfološke osobine plodova i sjemena i trajanje učinka skarifikacije sjemena sumpornom kiselinom na neke vrste iz roda Crataegus Tourn. ex L.

Hawthorn species (Crataegus Tourn. ex L.) are widely known for their medical, ecological, and economic benefits. The main issue of this study was to define the morphological characteristics of fruits and seeds as well as the impact of sulfuric acid (96% concentration) scarification with different time duration on seeds of three different hawthorn species (C. crus-galli, C. monogyna, C. laevigata). Statically significant differences were reported for all measured fruit and seed morphological traits. Highest fruit weight, length and width was recorded for C. crus-galli, while lowest for C. monogyna. Different sulfuric acid (96% concentration) scarification treatments were used for C. cruss-galli (150 and 210 min), C. laevigata, and C. cruss-galli (45, 75 and 105 min) seeds. Only significant differences were found in contrast to the control. Thickness of the tiniest part of the seed coat was significantly reduced in C. crus-gallic and C. laevigata scarified seeds in contrast to control. Thickness of the thickest part of the seed coat was significantly reduced only in C. crus-galli seeds scarified for 210 min in contrast to control. Therefore, optimal duration of scarification treatment for C. crus-galli seeds should be 210 min. The results obtained in this study will contribute to a better knowledge of morphometric characteristics and the pre-sowing treatment of seeds of some Crataegus species which can be utilized in nursery production.

Effectiveness of use of biological drugs and microelements in the cultivation of chickpeakes in light chestnut soils in the Volgograd region

Chickpeas are widespread in countries with arid climates, where they are sown annually on an area of 10–11 million hectares. The favorable combination of protein, fat, carbohydrates, macro- and microelements, vitamins, and biological active substances in grain make it a complete feed for farm animals and poultry. The purpose of the research was to study the eff ectiveness of the use of biological products and microelements when cultivating chickpeas under the conditions of light chestnut soils in the Volgograd region. In the process of work, two series of experiments were carried out: the fi rst – “Pre-sowing treatment of chickpea seeds with microfertilizers” and the second – “Combined use of growth stimulants and microfertilizers”. The most important conditions for the realization of chickpea productivity, taking into account the biological characteristics of the variety Privo 1 in the soil and climatic conditions of light chestnut soils of the Volgograd region have been identifi ed. In the second experiment, the average lowest economic yield of chickpea variety Privo 1 was formed in the control variant without seed treatment with microelements and growth stimulants and was equal to 1,92 t/ha. The highest economic yield of chickpeas, on average, according to experience, was established in combinations of the growth stimulator HB-101 with boric acid or ammonium molybdate during pre-sowing seed treatment and was equal to 2,59 t/ha, i.e. was 34,9 % higher than in the control variant. It was recommended that under the conditions of the Volgograd region on light chestnut soils when cultivating chickpea variety Privo 1, before sowing, treat chickpea seeds with growth stimulant HB-101 (50 ml/t) in combination with ammonium molybdate (NH4)2MoO4 – (250 g/t) or boric acid H3BO3, B(OH)3 (500 g/t). Sowing should be carried out as early as possible with a seeding rate of 500 thousand viable seeds on 1 ha.

Effectiveness of pre-sowing treatment with plant growth regulators to improve the productivity of <i>Astragalus babatagi</i> and <i>A. xanthomeloides</i>

In 2022, under the conditions of the growing experience of the IHR of the Academy of Sciences of the Republic of Uzbekistan on plants Astragalus babatagi and Astragalus xanthomeloides, optimal sowing dates and options for seed treatment with plant growth regulators and organo-mineral fertilizer were studied. The positive effect of pre-sowing seed treatment was revealed, which significantly affected the increase in seed productivity and plant phytomass. It was found that pre-sowing treatment of Astragalus babatagi seeds with Uchkun plus, Floroxan and potassium Humate Prompter preparations contributed to an increase in seed yield from one bed by 1.0, 0.98 and 0.8 g relative to scarified seeds and an increase in phytomass yield by 189, 197 and 186 g, respectively. The increase in the yield of Astragalus xanthomeloides seeds from one bed in experimental versions using Uchkun plus, Floroxan and potassium Humate Prompter increased by 0.92, 1.05 and 0.62 g, respectively, compared with the “scarification” option, and compared with the control by 1.82, 1.95 and 1.52 g, respectively. The yield of phytomass increased by 53.7, 61.2 and 30.7 g relative to the variant of scarified seeds, whereas in relation to the control these indicators increased by 103, 110 and 79.8 g, respectively.