Presence Ofa Research Articles

Modulation of Solid-state Thermal Reaction of Iron(III)Citrate by a Co-precursor Studied using Thermogravimetry: Evaluation of Kinetic and Thermodynamic Parameters and Nucleation Rate

Background: Solid state reaction of iron(III)citrate leads to a range of ironbased oxides by varying the reaction conditions, e.g., the presence of co-precursor. The influence of reaction conditions on the kinetics of the solid-state reaction of iron(III)citrate needs to be investigated. Objective: Kinetic analysis of the solid-state reaction of iron(III)citrate in the presence of a co-precursor has been explored to realize the influences of the co-precursor on the reaction process as well as decomposed material. Method:: Non-isothermal thermogravimetry profiles are deconvoluted to individual reaction steps. The model-free kinetic methodology is utilized to estimate step-wise activation energy and, hence, the reaction mechanism along with the reaction rate. Conversiondependent thermodynamic parameters and nucleation rate are estimated. XRD analysis has been used to characterize the decomposed material. Results: Thermogravimetry profiles obtained for an iron(III)citrate and malonic acid mixture are deconvoluted into six steps. The decomposed nanomaterial is identified as magnetite (size 10 nm). The observed reaction mechanisms associated with each step are different, where the activation/reaction rate is conversion-dependent. A good fit between the experimental and reverse-constructed conversion profiles is obtained. The nucleation rate at higher temperatures is affected by both the extent of conversion and the heating rate. A possible reaction pathway is proposed. The study elucidates the role of malonic acid as a co-precursor in modifying the thermal reaction of iron(III)citrate and product formation. Conclusion: This investigation proposes the applicability of suitable co-precursors as a potential controlling factor for preparing iron oxides from iron-based compounds.

The Madness of Mold: Ecogothic in Nathaniel Hawthorne’s The House of the Seven Gables

Nathaniel Hawthorne’s description of a New England mansion in The House of the Seven Gables (1851) relies on well-established gothic tropes that ghost certain environmental incidents and characters’ reactions to them. In turn, natural encroachment on human structures seems an otherworldly and unlikely phenomenon. This rhetorical move eases tension in stories where buildings symbolize U.S. success at mastering the landscape but also reveals architecture’s constant vulnerability to outdoor elements. This article examines Hawthorne’s depictions of decay to argue that widespread and resilient fungi, most usually mold, underscore human anxieties about the environment lurking amid portrayals of gothic structures like the famed seven-gabled house. Conditions of rot suggest the impermanence of buildings and, by extension, the limits of conquering the natural world, exhibiting an ecological reality that appears less troubling when represented through ghastly but impossible incidents. In Hawthorne’s novel, the haunting presence of fungal forms in materials, both inside the house and in the surrounding area, suggests failed ambitions and a passing socioeconomic status, so that the story transforms decay into spectral manifestation. An ecogothic reading thus highlights how sometimes unseen biological realities, such as conditions for fungal growth, enhance supernatural and existential horrors while also distracting from concerns about nature’s long-term damaging of human structures like houses. Like the mold it studies, this essay treats the psychology of domestic spaces (a major Hawthornean theme) as permeable by showing that the novel’s gloomy atmosphere, ghostly hauntings, alleged curses, and other fantastical events are enhanced by the presence of a veiled ecological catalyst.

EROSITA detection of a cloud obscuration event in the Seyfert AGN EC 04570$-$5206

Recent years have seen broad observational support for the presence of a clumpy component within the circumnuclear gas around supermassive black holes (SMBHs). In the X-ray band, individual clouds can manifest themselves when they transit the line of sight to the X-ray corona, temporarily obscuring the X-ray continuum and thereby indicating the characteristics and location of these clouds. X-ray flux monitoring with Spectrum Roentgen Gamma extended ROentgen Survey with an Imaging Telescope Array ( SRG /eROSITA) has revealed that in the Seyfert 1 active galactic nucleus (AGN) EC 04570$-$5206, the soft X-ray flux dipped abruptly for about 10--18 months over 2020--2021, only to recover and then drop a second time by early 2022. Here, we investigate whether these flux dips and recoveries could be associated with cloud occultation events. We complemented the eROSITA scans with multiwavelength follow-up observations, including X-ray/UV observations with Swift XMM-Newton and NICER, along with ground-based optical photometric and spectroscopic observations to investigate the spectral and flux variability. XMM-Newton spectra confirm that the soft X-ray flux dips were caused by partial-covering obscuration by two separate clouds. The 2020-2021 event was caused by a cloud with column density near $1 $ cm$^ $ and a covering fraction of roughly 60 percent. The cloud in the 2022 event had a column density near $ 3 $ cm$^ $ and a covering fraction near 80 percent. The optical/UV continuum flux varied minimally and the optical emission line spectra showed no variability in Balmer profiles or intensity. The transiting gas clouds are neutral or lowly-ionized, while the lower limits on their radial distances are commensurate with the dust sublimation zone (cloud 1) or the optical broad line region (cloud 2). One possible explanation is a dust-free, outflowing wind with embedded X-ray clumps. These events are the first cloud obscuration events detected in a Seyfert galaxy using eROSITA's X-ray monitoring capabilities.

Mitigation of post breeding endometritis in mares treated with platelet rich plasma

Persistent breeding-induced endometritis (PBIE) is one of the major causes of infertility in mares, characterized by a lingering inflammatory response in the uterus, which leads to accumulation of uterine fluid and polymorphonuclear cells (PMN), harming the reproductive efficiency of the animal. The aim of this study was to determine if the presence and volume of intrauterine fluid and the percent of endometrial neutrophils decreased after intrauterine application of blood platelet-rich plasma (PRP) in mares with post-mating endometritis. Healthy estrous crossbreed mares (n = 9) aged between 6 to 12 years, with optimal body condition and under the same nutritional management, had two cycles randomly assigned to a crossover trial. Each received either intrauterine infusions of lactate ringer solution (LRS) or autologous PRP 4h after artificial insemination (AI) with fresh semen of low sperm quality from the same stallion. All the animals went through all the treatments. A total of 100 mL of citrated whole blood was collected for PRP production and was centrifuged at 2315g for 15 minutes then 579g for 10 minutes. The presence of intrauterine fluid (IUF) was analyzed by ultrasonographic examination and the percentage of endometrial neutrophils was determined through endometrial cytology, both before insemination (in the presence ofa preovulatory follicle ≥35mm and endometrial edema) and 24h after treatment application. Statistical analysis was performed by a Mixed model (SAS, 2023) for repeated measures with treatment and time as between subject factors. The presence of intrauterine fluid had an effect of time (p ≤ 0.049), where fluid size was smaller before AI than 24h after treatment; however, there was no treatment effect (p ≥ 0.264) over intrauterine fluid. The percentage of endometrial neutrophils had an effect of time (p ≤0.001), less inflammatory cells were found before AI than 24 hours after treatment; there was an effect of treatment (p ≤0.001) as well, where PRP decreased the number of endometrial polymorphonuclear cells compared to the control group infused with LRS. The increase in intrauterine fluid with time suggests that semen itself increases blood perfusion that generates intrauterine fluid accumulation. After induction of physiological endometritis, endometrial polymorphonuclear cell numbers were reduced successfully with PRP treatment. PRP is rich in peptides and signaling proteins that suppress the expression of proinflammatory cytokines such as IL-1 and TNF, inhibits polymorphonuclear neutrophil migration, and thus regulates intrauterine inflammation through decreased endometrial COX-2 expression.

Characteristic features and comparative analysis of essential oil composition of selected genus of Ocimum sanctum L. through GC-MS.

The main aim of this research is to provide literature on the Ocimum plant, and to know the significance of the Ocimum species carried out by pharmacognostic study and experimental design for GC-MS. Ocimum genus are very important for their therapeutic potential among the most important aromatic herbs. Extreme attention has been put on literature reports in which the utilization of tulsi and their pharmacognostic study has been done by performing morphological and microscopic leaf experimental design and by using essential oil through the GC-MS instrumentation method. The utilization of these characteristics would be important for the drug discovery scientist to develop a specific formulation of the crude drug, which will be a magical therapeutic agent in the future, with many advantages. GC-MS chromatogram of Ocimum sanctum, Ocimum canum, and Ocimum gratissimum oil showed major peaks and has been identified after comparison of the mass spectra with the NIST library, indicating the presence of three phytocomponents. From the results, the GC-MS study suggested that anethole which is well reported antimicrobial compound is more in O. canum (2.66%) in comparison to O. sanctum (1,28%) but absent in O. gratissimum. The results indicated that the antimicrobial activity is more in O. canum due to the presence ofa high amount of anethole in comparison to O. gratissimum and O. sanctum. The result revealed that O. canum has a microscopic character that can be identified by the characteristic GC MS analysis of extracts to distinguish between different species of the ocimum plant.

A biogeographical approach to characterizing the climatic, physical and geomorphic niche of the most widely distributed mangrove species,Avicennia marina

AbstractAimMangroves are coastal ecosystems exposed to terrestrial, marine, geomorphic and climatic forcings operating in concert, making the mangrove niche hard to define, as evidenced by extremely poor restoration outcomes. We have developed a set of high‐resolution species distribution models and interpreted the variables that have the largest impact on the niche ofAvicennia marina, the most globally widespread mangrove species, to comprehensively detail the forcings driving habitat suitability.LocationAustralia.Time period1970–2020.Major taxa studiedAvicennia marina(Forssk.) Vierh.MethodsWe modelled the suitable habitat forA. marinain Australia using the maxent method incorporating 38 environmental variables and the Global Mangrove Watch baseline for the presence records. Using k‐means grouping, we identified subregions where similar suites of environmental variables influence habitat suitability, while also identifying biogeographical commonalities among the subregions. To better understand the low realization of the fundamental niche, we analysed the other land covers occupying the niche.ResultsAvicennia marinain Australia occupies six different environmental subregions. Maxent distribution models accurately predicted the presence ofA. marinain each subpopulation (AUC > 0.9).A. marina's presence in all subregions was strongly determined by its proximity to freshwater. Precipitation and temperature extreme values were more important than average values in predicting the species presence. The species requires low‐energy coastlines with high solar radiation. The suitable areas are primarily shared with salt marshes, seagrass and buildings or cleared land.Main conclusionsOur results offer a baseline for the suitable area ofA. marina's presence that includes a range of environmental conditions,A. marinacurrently occupies <50% of its suitable habitat, and there is scope for restoration with significant ecosystem service gains. The six different subregions in Australia map to known phylogenetically distinct populations, indicating genetic plasticity in response to region specific climatic conditions.

Determination of Hydrodynamic Parameters of Chitosan Stabilized Bimetallic Nanoparticles

The hydrodynamic characteristics of bimetallic Ag/Cu and Co/Ag nanoparticles stabilized by chitosan were determined. The polydispersity indexand the diameter of nanoparticles were observed to decrease in contrast tothe original polymer during the creation of chitosan stabilized bimetallicnanoparticles, decreasing from 0.342 to 0.12±0.04 and 2.5 micron to 180nm, respectively. However, the diffusion coefficient of chitosan was increased from 0.2 cm2 /s to 2.71 cm2 /s during the production of stable bimetallic nanoparticles. The lack of absorption bands at 500 nm and 700 nm-900 nm in the UV spectra of the samples suggests that in the presence ofa reducing agent, copper (II) and cobalt (II) ions undergo full reduction.The relationship between the synthesis conditions and the kind of structureof bimetallic nanoparticles “core-shell” has been discovered. Silver atomshave been shown to be both a core and a shell, depending on the synthesisconditions and chemical nature of metal ions.

Hypoxic-ischemic brain injuries in cardiosurgical patients (rationale of causal connections)

Summary. The presented article substantiates the materials on causation in the most common variants of hypoxic-ischemic brain lesions in cardiac surgery patients. The study showed that the functional changes assumed by the patient's brain (under the conditions of heart surgery using the resources of artificial circulation), reflects the state of the structural patterns of the whole organism. The aim of the study – to analyze the clinical and anamnestic materials on pathogenetic prototypes of hypoxic-ischemic lesions that occur in patients as a result of cardiac surgery. Materials and Methods. The materials were the results of anamnestic profile clinical examinations and observations of patients (n = 118) who were on routine hospitalization in the profile department of the State Institution "Heart Institute of the Ministry of Health of Ukraine" in Kyiv. The study used a thematic map of the patient, developed and processed by the author. Its content was related to clinical prototypes associated with the leading symptoms of the disease and contributed to the understanding of the pathogenetic mechanisms of the latter. Results. The complex research, scientific-theoretical, methodological, and patent-licensing information on the specialty has shown that embolism is considered to be a priority mechanism for the development of hypoxic-ischemic lesions. Another important factor in the pathogenesis of hypoxic-ischemic disorders is hypoperfusion. One of the reasons for the development of stroke during cardiac surgery, especially in the surgical treatment of aortic pathology, is hypoperfusion due to instability of central hemodynamics. The next level of influence on systemic processes of development of hypoxic-ischemic defeats of the brain of cardiac surgery patients is the systemic inflammatory response. According to some researchers, CNS damage after cardiac surgery is largely due to side effects associated with the use of artificial circulation. An important point associated with the occurrence of hypoxic-ischemic changes in the brain is called general anesthesia. The technique of total intravenous and/or inhalation anesthesia does not provide adequate protection of CNS structures from intraoperative pain. Conclusions. Analysis of clinical and anamnestic materials on pathogenetic prototypes of hypoxic-ischemic lesions that occur in patients as a result of cardiac surgery, shows the presence of a complex of pathogenetic components, among which the leading role is played by: embolism; hypoperfusion; systemic inflammatory response with changes in hemostasis and the use of an arsenal of general anesthesia

Green Synthesis of Benzoimidazopyrimidine, Benzimidazoloquinazolinone, Triazolopyrimidine and Triazoloquinazolinone Derivatives Catalyzed by Oxalic Acid Dihydrate

Abstract: Benzoimidazopyrimidine, benzimidazoloquinazolinone, triazolopyrimidine, and triazoloquinazolinone derivatives were synthesized via a one-pot multi-component reaction in the presence of a catalytic amount of oxalic acid dihydrate. The reactions were performed in the mixture of EtOH:H2O or under solvent-free conditions as green media. The advantageous features of these methodologies are inexpensive starting materials and catalyst, high atom economy and good yields, and metal-free synthesis. Moreover, all the products were obtained by simple filtration, and no need for column chromatography or tedious separation procedures, which is very important in terms of reducing environmental pollutions.

Er3+ Incorporated Transparent Ternary Nanocomposite as Active Core Material in Polymer Optical Preform with Improved Photo-emission Performance

Background: A polymer as a host in the optical waveguide has many advantages and, when doped with rare-earth (RE) elements, offers an efficient connection, compared to its glassbased counterparts as an amplifier. However, a polymer matrix causes the concentration quenching effect of REs in the polymer matrix, making the fabrication of RE-doped polymer waveguides more complicated as compared to the fabrication of glass-based complements. Moreover, controlling scattering loss at the particle-polymer interface for maintaining the optical clarity of the composite is also a great challenge. Objective: The main aim of the present study was to optimize the synthesis of Er2O3 grafted Polymethylmethacrylate (PMMA)-Polystyrene (PS) composite based transparent ternary nanocomposite and its characterization to implement them as a potential material for active core in Polymer Optical Preform (POP). Methods: Nano Erbium Oxide (Er2O3) was successfully synthesized by the wet-chemical method and encapsulated by a polymerizable surfactant, i.e., 3-Methacyloxypropyltrimethoxy silane (MPS). The encapsulated nanoparticles were further subjected to grafting with PMMA using in-situ polymerization of methyl methacrylate (MMA) followed by blending with PS via solvent mixing technique. Results: The optical transparency of the ternary composite was achieved by fine-tuning the diameter (15-20 nm) of the PMMA coated Er2O3 . The crystallinity present in Er2O3 was significantly reduced after PMMA coating. The comparatively higher refractive index obtained at 589 nm wavelength for the synthesized material indicated its usability as active core material in the presence of a commercial acrylate cladding tube. A photoluminescence (Pl) study indicated that the technique might be used for a higher level of Er3+ doping in polymer matrix without sacrificing its transparency. Conclusion: The obtained results indicated that the sample synthesized with the adopted technique gives better Pl intensity compared to the other methods of Er3+ incorporation in polymer optical preform (POP).

Homogeneous pressure hydrogenation of quinolines effected by a bench-stable tungsten-based pre-catalyst

We report on an operationally simple catalytic method for the tungsten-catalyzed hydrogenation of quinolines through the use of the easily handled and self-contained precursor [WCl(η5-Cp)(CO)3]. This half sandwich complex is indefinitely storable on the bench in simple screw-capped bottles or stoppered flasks and can, if required, be prepared on a multi-gram scale while the actual catalytic transformations were performed in the presence ofa Lewis acid in order to achieve both decent substrate conversions and product yields. The described method represents a facile and atom-efficient access to a variety of 1,2,3,4-tetrahydroquinolines that circumvents the use of cost-intensive and oxygen-sensitive phosphine ligands as well as auxiliary hydride reagents.

Improving risk stratifcation of pulmonary hypertension patients

Abstract Background According to the 2016 ESC/ERS Guidelines on Pulmonary Hypertension (PH), the right atrial area (RAA) and the presence ofa pericardial effusion (PE) are the two main echocardiographic prognostic markers in PH patients (pts). Aim To assess the predictive ability of these two parameters. Methods Pts with PH were prospectively studied and several clinical/demographic/echocardiographic were retrieved as well as data from six-minute walk test (6MWT) and brain natriuretic peptide (BNP). All-cause mortality was analyzed by PE, RAA and other echocardiographic parameters for positive (PPV) and negative predictive value (NPV) to detect if the current guideline recommended cut-offs can precisely stratify risk in this setting. A survival analysis was performed to evaluate risk stratification (RS) provided by several different cut-offs. Results A total of 51 PH pts (mean age 54±46 years, 33.3% male, baseline BNP of 342.4±439.9pg/mL, mean 6MWT distance of 360.3±109.2 meters and baseline pulmonary artery systolic pressure of 78±26mmHg), of which 64.7% had Group I PH (GI) and 35.3% presented chronic thromboembolic pulmonary hypertension. There were no significant differences between these two groups, however pts in GI were significantly younger (p=0.001), achieved a lower 6MWT distance (p=0.038) and had worse values of right ventricular strain (p=0.040). 27 pts (52.9%) died during a mean follow-up of 52 months, with no differences between groups (p=0.756). The presence of a PE had a low NPV and PPV for the primary endpoint (45.0% and 45.5%, respectively), as well as the guideline recommended cut-offs for RAA (18cm2: NPV- 50.0% and PPV– 55.2%; 26cm2: NPV- 51.3% and PPV– 66.7%). A Pulsed Doppler Tei index (TIp) cut-off of 0.40 had a higher NPV (70.8%) and PPV (74.1%). By Kaplan-Meieran alysis, neither the presence of PE (log rank p=0.508) nor the recommended RAA cut-offs provided accurate risk discrimination (log rank p>0.05 for all). Pts below a TIp cut-off of 0.40 presented a significantly lower survival during follow-up (log rank p=0.002) Conclusion The currently recommended echocardiographic prognostic markers cannot precisely discriminate risk in PH pts. Markers of Right Ventricular Dysfunction may improve RS in this population. Funding Acknowledgement Type of funding sources: None.

Effects of a floating wave barrier with square cross section on the wave-induced forces exerted to an offshore jacket structure

The main objective of the present research was investigating the effects of a floating wave barrier with square cross section installed in front of an offshore jacket structure on the wave height, base shear, and overturning moment. A jacket model with the height of 4.55 m was fabricated and tested in the 402 m-long wave flume of NIMALA marine laboratory. The jacket was tested at the water depth of 4m subjected to the random waves with a JONSWAP energy spectrum. Three input wave heights were chosen for the tests: 20 cm, 23 cm, and 28 cm. Results showed that the average decrease in the jacket' s base shear due to the presence of a floating wave barrier with square cross section was 18.97%. The use of wave barriers with square cross section also resulted in 19.78% decrease in the jacket' s overturning moment. Hence, it can be concluded that a floating wave barrier can significantly reduce the base shear and overturning moment in an offshore jacket structure.

Measurement report: Effect of wind shear on PM<sub>10</sub> concentration vertical structure in the urban boundary layer in a complex terrain

Abstract. The paper shows wind shear impact on PM10 vertical profiles in Kraków, southern Poland. The data used consist of background data for two cold seasons (September 2018 to April 2019 and September 2019 to April 2020) and data for several case studies from November 2019 to March 2020. The data are composed of PM10 measurements, model data, and wind speed and direction data. The background model data come from operational forecast results of the AROME model. PM10 concentration in the vertical profile was measured with a sightseeing balloon. Significant spatial variability of the wind field was found. The case studies represent the conditions with much lower wind speed and a much higher PM10 level than the seasonal average. The inversions were much more frequent than on average too. Wind shear turned out to be the important factor in terms of PM10 vertical profile modification. It is generated due to the relief impact, i.e. the presence of a large valley, blocked on one side with the hills. The analysis of PM10 profiles from all flights allows us to distinguish three vertical zones of potential air pollution hazards within the valley (about 100 m deep) and the city of Kraków: (1) up to about 60 m a.g.l. – the zone where during periods of low wind speed, air pollution is potentially the highest and the duration of such high levels is the longest, i.e. the zone with the worst aerosanitary conditions; (2) about 60–100 m a.g.l. – transitional zone where the large decrease in PM10 levels with height is observed; (3) above 100–120 m a.g.l. – the zone where air quality is significantly better than in zone 1, either due to the increase in the wind speed or due to the wind direction change and advection of different, clean air masses.

FLT3 Mutation in Acute Myeloid Leukemia: Primary or Secondary Mutation?

The receptor tyrosine kinase FLT3 gene is commonly mutated in acute myeloid leukemia (AML) and its mutation is associated with significantly more aggressive disease and poor outcome. However, in the co-presence of NPM1 mutation, the disease is less aggressive and is associated with better outcome. FLT3 inhibitor midostaurin (Rydapt; Novartis Pharmaceuticals, Inc.) was recently approved for patients with FLT3 mutations. While the presence ofa FLT3 mutation is commonly associated with AML,it can develop in less acute myeloid neoplasms, such as MDS and CMML, and implies transformation into more acute phase. In this context, the FLT3 mutation is typically detected in a subclone of the original chronic myeloid clone. The prevalence of subclonal FLT3 mutations is unknown, as is the clinical relevance of subclonal FLT3, especially when treated with FLT3 inhibitors. We used variant allele frequency (VAF) and next generation sequencing (NGS) to determine if FLT3 mutations are founding or secondary mutations and to characterize the co-mutational background in large number of consecutive patients who presented with a clinical impression of myeloid neoplasm.Methods: A total of 6390 consecutive bone marrow aspirate samples or peripheral blood samples were submitted with clinical impression of AML, MDS, or MPN between January 2014 and mid 2017 were tested for mutations in myeloid genes using NGS. We used the TruSight Myeloid panel (Illumina, San Diego, CA) for detecting missense mutations and fragment length analysis (FLA) for detecting internal tandem repeat (ITD) in FLT3 . DNA was extracted from samples using the QIAamp DNA Mini Kit. This NGS testing covers mutations in 54 myeloid-related genes. The average depth of sequencing was 10,000x.Results: A total of 2670 (42%) of the samples showed a mutation in one or more of the tested genes. Of the samples with mutations, FLT3 ITD and kinase domain (KD) mutations were detected in 313 (12%) patients. Of these patients, 208 (66.5%) had ITD and 117 (37.4%) had KD mutations. Twelve patients (3.8%) had both ITD and KD mutations. NPM1 mutation was detected in 138 (44%) of the patients. Most of FLT3 -KD mutations were in codon 835, but mutations in codons 836, 839, 842 and 845 were also found. Additionally, missense mutations in codons 576, 592, and 593 at the site of ITD were detected. To distinguish a FLT3 mutation as a founding mutation rather than a secondary mutation, we used a lower VAF of 10% as a cut-off. Using this approach, we found that 27% (115/313) of patients had secondary FLT3 mutations. Of these subclonal FLT3 mutants, NPM1 co-mutation was detected in 61 (53%) patients, but was not statistically significant (P=0.06). Patients with FLT3 mutation as a subclone had a significantly higher number of co-mutated genes (P-value <0.0001). This group of patients had higher (P≤0.01) mutations in TET2, DNMT3A, NRAS, IDH2, EZH2, and BCOR, consistent with an MDS/CMML background. On the other hand, patients with both FLT3 and NPM1 mutations had a significantly lower number of co-mutant genes (P<0.0001) and particularly lower (P≤0.01) mutation rate in U2AF1, SF3B1, SRSF2, RUNX1, IDH1, BCOR, and AXL1 .Conclusion: FLT3 mutations are frequently (27%) a secondary mutation in a subclone of the neoplastic founding mutations. This appears to be more common when FLT3 mutation is developing in a background of MDS/CMML. The co-presence of an NPM1 mutation is more commonly associated with fewer co-mutations in MDS-related genes. This data suggests that in addition to detecting ITD by FLA, using NGS for profiling mutations in FLT3 -KD and other myeloid genes should be considered whenever FLT3 mutations are evaluated. Further, the clinical decision for combination therapy should consider the entire mutation profile and not only mutations in FLT3 . [Display omitted] DisclosuresMa:NeoGenomics: Employment. De Dios:NeoGenomics: Employment. Funari:NeoGenomics: Employment. Sudarsanam:NeoGenomics: Employment. Jiang:NeoGenomics: Employment. Agersborg:NeoGenomics: Employment. Hummel:NeoGenomics: Employment. Blocker:NeoGenomics: Employment. Albitar:NeoGenomics: Employment.

First Detection of the Crayfish Plague Pathogen Aphanomyces astaci in Costa Rica: European Mistakes Should Not Be Repeated

The crayfish plague pathogenAphanomyces astaciis one of the main factors responsible for the decline in European and Asian native crayfish species. This pathogen was transported to these regions through its natural carriers, North American crayfish species, which were introduced during the last century. Since then, the carrier species and the pathogen have spread worldwide due to globalization and the highly invasive nature of these species. In Europe, five carrier species have been categorized as high-risk as they are responsible for the loss of provisioning services, which endangers freshwater ecosystems. The red swamp crayfishProcambarus clarkii, in particular, is currently one of the most concerning species as its spread threatens crayfish biodiversity and freshwater ecosystems worldwide. In this study, we describe the first detection ofA. astaciin an introduced population ofP. clarkiiin Central America, specifically in Costa Rica. Using molecular approaches, we analyzed 48 crayfish samples collected from Reservoir Cachí and detected the presence ofA. astaciin four of these samples. The introduction ofP. clarkiiand the incorrect management of the species (related to its fishery and the commercialization of live specimens) over the past decades in Europe are mistakes that should not be repeated elsewhere. The detection of the pathogen is a warning sign about the dangerous impact that the introduction of this invasive crayfish may have, not only as a carrier of an emerging disease but also as a direct risk to the invaded ecosystems. Our results may serve to (1) assess current and future consequences, and (2) direct future research activities, such as determining the potential impacts ofA. astacion native decapod species, or on other introduced crayfish species that are used for aquaculture purposes, such asCherax quadricarinatus.

Rehabilitating an Ocular Defect with a Custom Made Ocular Prosthesis: A Clinical Report

An ocular prosthesis is a replacement of the ocular part of the eye in patients due to trauma, benign ormalignant lesion or congenital anomaly, there is the loss of the eye. Prosthetic rehabilitation with a customizedocular prosthesis that is proportioned and similar in appearance to that of the contralateral natural eye isneeded. This maintains the volume of the orbital socket and also helps create an illusion of the presence ofa healthy eye. It provides the individual confidence to adapt to a normal lifestyle and prevent them fromgoing into psychological distress. Various techniques have been advocated for the fabrication of the ocularprosthesis comprising the use of the stock prosthesis, custom made prosthesis and micro-implants for therehabilitation of the ocular part of the eye. A cosmetically approvable and pleasing ocular prosthesis arethose which reproduces the colour, appearance and orientation of the iris to that of the contralateral naturaleye. This clinical report describes a simple, economical and time-saving method for the fabrication of thecustomised ocular prosthesis in a patient with the ocular defect.

Dipolar analysis of substrated particles using a far-field response method

The presence of a substrate, when working with nanoparticles, is essential in many applications, like optical nanoantennas, solar cells, and sensing. Understanding the effects of substrates upon the nanoparticles is, therefore, important, as the substrates typically affect the resonance behaviors of particles, as well as the interactions between their electric and magnetic resonances. In order to better understand the impacts of substrates in practical applications with nanoparticles, this paper presents a semianalytical method to calculate the polarizability tensors of individual nanoparticles located on dielectric substrates. This approach is based on a sampling of the scattered far-field responses to plane-wave illuminations from structures. By using scattered far fields, the induced electric and magnetic dipole moments are calculated at the geometrical center of a particle. Then, using these dipole moments, the individual polarizability tensors of the substrated particle are calculated. To show the accuracy of the proposed method, the numerical results of different particles on a substrate are compared to two other approaches, and the results are shown to be in good agreement with these approaches. Moreover, the effect of the refractive index of the substrate and the geometric characteristics of the particle on the substrate-induced bianisotropy are also investigated. The proposed method clearly demonstrates how a particle without any bianisotropic interaction in free space can possess this property in the presence of a dielectric substrate.

Czynności notarialne online – podstawy de lege lata i uwagi de lege ferenda

Abstrakt zostanie przesłany w środę

Analisis Biaya dengan Menggunakan Metode Break Even Point dalam Mencari Volume-Laba pada PT X

For companies whose activities sell goods, income is strongly influenced by the volume of sales. Thepurpose of an enterprisein general is looking for profit, the size of the profit that will beachieved will bethe measure of success in the processing of management company, it required the presence ofa planning. Corporate planning can be done in various ways, including the program budget containingthe estimated income to be earned and the costs that will occur to those who earn income eventually beachieved.The program budget itself will be more beneficial to the management if accompanied by planningtechniques onother analysis, for example by using break-even analysis,because to know the size of thebreakeven necessary to make analysis of the relationship between cost, volume, selling price and profit. Break-even can be interpreted a state where the company does not earn a profit and doesnot suffer loss. Break evenanalysis is able to provide information to business leaders on various levels of sales, as well as itsrelationship with the possibility of obtaining a profit according to the level of sales is concerned. With the break-even analysis of the leadership can know how much income the production volume can cover the total cost. Socompanies can avoid losses. From the results of data collection and processing can be in the know break evenpoint, the selling price per unit, cost perunit in each year, as well is profit projections for future years usingthe method of smoothing (average). Variable costs and sales volume annually.