You have accessJournal of UrologyStone Disease: Basic Research I1 Apr 2014MP20-08 TRANSIENT RECEPTOR POTENTIAL A1-MEDIATED SIGNALS MODIFY IN VIVO AUTONOMOUS PERISTALTIC URETER ACTIVITY IN RATS Philipp Weinhold, Luca Villa, Frank Strittmatter, Jan Franken, Christian Stief, Christian Gratzke, Francesco Montorsi, Fabio Benigni, and Petter Hedlund Philipp WeinholdPhilipp Weinhold More articles by this author , Luca VillaLuca Villa More articles by this author , Frank StrittmatterFrank Strittmatter More articles by this author , Jan FrankenJan Franken More articles by this author , Christian StiefChristian Stief More articles by this author , Christian GratzkeChristian Gratzke More articles by this author , Francesco MontorsiFrancesco Montorsi More articles by this author , Fabio BenigniFabio Benigni More articles by this author , and Petter HedlundPetter Hedlund More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.730AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The transient receptor potential A1 (TRPA1)-ion channel is involved in mechanochemo sensation of the lower urinary tract. TRPA1-expression, and modulation of nerve activities by TRPA1-agonists in the human isolated ureter is described. It is not known if TRPA1-activation in vivo modifies ureter functions. This study aimed to test if the TRPA1-agonists NaHS (donor of H2S, a metabolite of uropathogens) or cinnamaldehyde (CA) had effects on rat ureter perstalsis in vivo. METHODS After ethical approval, 22 anesthetized (isoflurane) male Sprague Dawley rats (250-300 gram) received a polyethylene (PE)-10 catheter in their left ureter just beneath the renal pelvis. Ureter pressure was recorded during intraluminal infusion (0.4ml/hour; corresponding to normal urine production) of saline containing 10 mg/ml of protamine sulfate (PS) to disrupt the urothelium. Subsequently, either NaHS (10mM) or CA (10mM) was administered by intraluminal infusion. A t-test was used for comparisons. Values are given as mean ± SEM. RESULTS All ureters had baseline rhythmic spontaneous contractile activity recorded as fluctuations in intraluminal pressure. Infusion of NaHS (n=8) decreased the frequency of the spontaneous contractions from 6±0.9 (baseline) to 2±0.3 contractions per minute (p=0.001), and increased the amplitude from 7±0.7 (baseline) to 10±0.8 cmH2O (p=0.06). Maximal pressure increased from 30±3.7 (baseline) to 38±4.7 cmH2O (p<0.001) after NaHS. The minimum pressure increased from 23±3.5 (baseline) to 28±4.7 cmH2O (p<0.01). The area under the curve (AUC) increased by NaHS from 26±3.6 (baseline) to 31±4.7 cmH2O.sec-1 (p<0.01). Infusion of CA (n=7), decreased the frequency of contractions from 7±0.5 (baseline) to 3±0.6 contractions per minute (p<0.001) and increased the amplitude from 7±2 (baseline) to 10±1.5 cmH2O (p=0.16). Maximal pressure increased from 38±8.6 (baseline) to 45±7.9 cmH2O (p<0.05). The minimum pressure increased from 30±6.8 (baseline) to 35±7 cmH2O (CA; p<0.05). The area under the curve (AUC) was increased from 33±7.7 (baseline) to 38±7.4 cmH2O.sec-1 (p<0.05) during infusion with CA. In separate experiments (n=7) using saline without PS, NaHS, but not CA, displayed similar effects as in the presence of PS. CONCLUSIONS Spontaneous rat ureter activity in vivo was significantly modified by TRPA1-agonists. It may be speculated that TRPA1-mediated signals are involved in mechanoafferent functions of the ureter in infection and upon urothelial damage in e.g. stone disease. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e198-e199 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Philipp Weinhold More articles by this author Luca Villa More articles by this author Frank Strittmatter More articles by this author Jan Franken More articles by this author Christian Stief More articles by this author Christian Gratzke More articles by this author Francesco Montorsi More articles by this author Fabio Benigni More articles by this author Petter Hedlund More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...