Presence Of Pathogenic Organisms Research Articles

Stock Health of Penaeid Shrimps in Sri Lanka: a Comprehensive Molecular-Based Study on Seven Diseases Found in the Asian Region

Purpose: Although shrimp aquaculture in Sri Lanka has mainly been plagued by recurrent outbreaks of White Spot Syndrome Virus (WSSV), recent symptomatic and analytical field data of shrimps were incoherent with typical WSSV infections. As transboundary infection of new diseases is not uncommon in world shrimp aquaculture, the possibility of new diseases established in the country surfaced. Hence, the present study was aimed at evaluating the stock health of shrimps in Sri Lanka for additional six diseases commonly available in the Asian region, namely Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV), Infectious Myonecrosis Virus (IMNV), Taura Syndrome Virus (TSV), Yellow Head Virus (YHV), Acute Hepatopancreatic Necrosis (AHPND) and Necrotizing Hepatopancreatitis Bacterium (NHPB). Research method: Sampling was performed using broodstocks, postlarvae, cultured Penaeus monodon, wild P. semisulcatus, P. indicus and P. monodon from different locations and broodstock collecting areas in the country. Other crustacean species were also collected as possible carrier species. The presence of pathogenic organisms was identified by Polymerase Chain Reaction using respective IQ 2000TM test kits and a total of 2060 PCR tests were conducted. Findings: Of all the samples tested, no samples were positive for IMNV, TSV, YHV, AHPND and NHPB. However, confirmatory evidence was found in Sri Lanka for the presence of IHHNV with the prevalence of 31.3% in broodstock samples, 24.07% in hatchery-produced postlarvae and 18.03% in cultured shrimps. WSSV was also recorded with a prevalence of 10.34% in broodstock samples and 55.56% in cultured shrimp samples collected. None of the wild collected samples was positive for any of the tested diseases. Value: Reinstating the presence of WSSV in the Sri Lankan shrimp industry, provides confirmatory evidence of the presence of IHHNV in the country, highlighting the possibility of the emergence of new diseases in shrimp farming in Sri Lanka. Also, commonly suggested disease carriers seem to be not the major cause of disease spread during the sampling time. This study, therefore, provides much-needed information about the stock health of shrimps in Sri Lanka.

Impact of perioperative airway pathogens on living-donor lobar lung transplantation outcomes.

To elucidate the clinical impact of pathogenic organism (PO) positivity early after transplantation, we evaluated the impact of perioperative airway POs on outcomes after living-donor lobar lung transplantation (LDLLT), where the graft airway is supposed to be sterile from a healthy donor. A retrospective review of 67 adult LDLLT procedures involving 132 living donors was performed. Presence of POs in the recipients' airways was evaluated preoperatively and postoperatively in intensive-care units. POs were detected preoperatively in 13 (19.4%) recipients. No POs were isolated from the donor airways at transplantation. POs were detected in 39 (58.2%) recipients postoperatively; most were different from the POs isolated preoperatively. Postoperative PO isolation was not associated with short-term outcomes other than prolonged postoperative ventilation. The 5-year overall survival was significantly better in the PO-negative group than in the PO-positive group (89.1% vs. 63.7%, P = 0.014). In the multivariate analysis, advanced age (hazard ratio [HR]: 1.041 per 1-year increase, P = 0.033) and posttransplant PO positivity in the airway (HR: 3.684, P = 0.019) significantly affected the survival. The airways of the living-donor grafts were microbiologically sterile. PO positivity in the airway early after transplantation negatively impacted long-term outcomes.

Performance evaluation of InfectID-BSI: A rapid quantitative PCR assay for detecting sepsis-associated organisms directly from whole blood

BackgroundBloodstream infections (BSIs) (presence of pathogenic organism in blood) that progress to sepsis (life-threatening organ dysfunction caused by the body's dysregulated response to an infection) is a major healthcare issue globally with close to 50 million cases annually and 11 million sepsis-related deaths, representing about 20% of all global deaths. A rapid diagnostic assay with accurate pathogen identification has the potential to improve antibiotic stewardship and clinical outcomes. MethodsThe InfectID-Bloodstream Infection (InfectID-BSI) test is a real-time quantitative PCR assay, which detects 26 of the most prevalent BSI-causing pathogens (bacteria and yeast) directly from blood (without need for pre-culture). InfectID-BSI identifies pathogens using highly discriminatory single nucleotide polymorphisms located in conserved regions of bacterial and fungal genomes. This report details the findings of a patient study which compared InfectID-BSI with conventional blood culture at two public hospitals in Queensland, Australia, using 375 whole blood samples (from multiple anatomical sites, eg. left arm, right arm, etc.) from 203 patients that have been clinically assessed to have signs and symptoms of suspected BSI, sepsis and septic shock. FindingsInfectID-BSI was a more sensitive method for microorganism detection compared with blood culture (BacT/ALERT, bioMerieux) for positivity rate (102 vs 54 detections), detection of fastidious organisms (Streptococcus pneumoniae and Aerococcus viridans) (25 vs 0), detection of low bioburden infections (measured as genome copies/0.35 mL of blood), time to result (<3 h including DNA extraction for InfectID-BSI vs 16 h–48 h for blood culture), and volume of blood required for testing (0.5 mL vs 40–60 mL). InfectID-BSI is an excellent ‘rule out’ test for BSI, with a negative predictive value of 99.7%. InfectID-BSI's ability to detect ‘difficult to culture’ microorganisms re-defines the four most prevalent BSI-associated pathogens as E. coli (28.4%), S. pneumoniae (17.6%), S. aureus (13.7%), and S. epidermidis (13.7%). InterpretationInfectID-BSI has the potential to alter the clinical treatment pathway for patients with BSIs that are at risk of progressing to sepsis.

Killing Bacteria by Faradaic Processes through Nano-Hydroxyapatite/MoOx Platforms.

Following the secular idea of ″restitutio ad integrum″, regeneration is the pursued option to restore bones lost after a disease; accordingly, complementing antibiotic and regeneration capacity to bone grafts represents a great scientific success. This study is a framework proposal for understanding the antimicrobial effect of biocompatible nano-hydroxyapatite/MoOx (nano-HA/MoOx) platforms on the basis of their electroactive behavior. Through cyclic voltammetry and chronoamperometry measurements, the electron transference capacity of nano-HA and nano-HA/MoOx electrodes was determined in the presence of pathogenic organisms: Pseudomonas aeruginosa and Staphylococcus aureus. Faradaic processes were confirmed and related to the switch of MoO42-/PO43- groups in the original hexagonal nano-HA crystal lattice and to the extent of OH vacancies that act as electron acceptors. Microscopic analysis of bacteria's ultrastructure showed a disruptive effect on the cytoplasmic membrane upon direct contact with the materials, which is not evident in the presence of eukaryotic cells. Experiments support the existence of a type of extracellular electron transfer (EET) process that alters the function of the bacterial cytoplasmic membrane, accelerating their death. Our findings provide strong quantitative support for a drug-independent biocidal physical approach based on EET processes between microorganisms and phosphate ceramics that can be used to combat local orthopedic infections associated with implants.

ANALYSIS OF THE BIOACTIVE AND MICROBIOLOGICAL CONTENTS OF HERBAL MIXTURES SOLD IN ANAMBRA STATE, NIGERIA

Over the years, medicinal plants have played a significant role in traditional medicine practice. The ever-increasing health-related difficulties and the search for possible treatments and likely cures have led to the increasing patronage of herbal drugs in Nigeria. There is an overwhelming increase in the production and distribution of herbal medications, and the trend has instigated concern about the quality of the products being sold in Anambra State, Nigeria. Thus, the primary purpose of the current study is to analyze the microbiological and phytochemical constituents of okanga powder, goko cleanser, and deep root herbal mixtures popularly sold in Nigeria. The phytochemical tests conducted on the herbal products revealed that the herbal products contain alkaloids, flavonoids, glycosides, anthraquinones, tannins, and saponins. The result of the microbial count indicates the presence of varying microorganisms such as S. aureus, E. coli, Salmonella, and Klebsiella spp in the herbal products. The study concludes that herbal products possess active phytochemicals. However, the presence of pathogenic organisms was discovered in the herbal drugs.

MICROBIOLOGICAL AND PHYTOCHEMICAL ANALYSES OF SOME SELECTED HERBAL MIXTURES SOLD IN NIGERIA

Over the years, medicinal plants have played significant role in traditional medicine practice. The rise in health-related problems, and the search for potential treatments and possible cures has led to the increased patronage of herbal drugs in Nigeria. there is an overwhelming increase in the production and distribution of herbal drugs and the trend has called for concern on the quality of the products been sold in Nigeria. thus, the primary purpose of the present study is to analyze the microbiological and phytochemical constituents of Okanga powder, Goko cleanser and Deep root herbal mixtures popularly sold in Nigeria. The phytochemical tests conducted on the herbal products revealed that the herbal products contain alkaloids, flavonoids, glycosides, anthraquinones, tannins, and saponins. The result of the microbial count indicates the presence of varying microorganisms such as S. aureus, E. coli, Salmonella and Klebsiella sppin the herbal products. The study concludes that the herbal products possess active phytochemicals, however, the presence of pathogenic organisms were discovered in the herbal drugs.

CELULITE EM FRANGOS DE CORTE – REVISÃO

A avicultura brasileira alcançou, nos últimos anos, níveis de produtividade e de ajuste na sua organização que a colocam como uma das mais competitivas do mundo. O resultado é um produto com qualidade, sanidade, sustentabilidade, que, aliadas a preços competitivos, levou o frango brasileiro a estar presente em mais de 150 países. O objetivo deste trabalho foi fazer uma revisão sobre a celulite aviária associada a ocorrência de Escherichia coli, destacando - se a etiopatogenia, o diagnóstico e a prevenção. Devido a fina espessura da pele dos frangos, vários fatores como deficiência nutricional, substâncias irritantes, toxinas, infecções e problemas de manejo podem potencialmente levar lesões cutâneas. A Escherichia coli é bactéria do grupo coliforme que fermenta a lactose e manitol, com produção de ácido e gás a 44,5 ± 0,2oC em 24 horas, produz indol a partir do triptofano, oxidase negativa, não hidrolisa a uréia e apresenta atividade das enzimas ß galactosidase e ß glucoronidase, sendo considerada o mais específico indicador de contaminação fecal recente e de eventual presença de organismos patogênicos. Portanto, a celulite tendo a E. coli como agente causador pode ser considerada como uma das mais importantes causas de condenação da carne de frango, gerando interesses em termos econômicos por conta das perdas financeiras impostas aos produtores. Além das perdas econômicas, há os riscos relativos à saúde pública, tornando-se necessária a reavaliação dos critérios de condenação em casos de celulite aviária determinados na legislação.

High Burden of Antibiotic-Resistant Bacteria from Wastewater in Ethiopia: A Systematic Review.

BackgroundCurrently, antibiotic-resistant bacteria (ARB) have become a serious global problem and considered as One Health challenge. Despite, wastewater contains a wide range of microbial pathogens and plays a significant role in the dissemination of ARB in the environment. However, it is the most overlooked in developing countries, particularly in Ethiopia.MethodsDifferent article searching devices like PubMed, Web of Science, Scopus, and Google Scholar were used to select research article by using the key terms. Hand search using a reference list is also used to retrieve the article. Preferred reporting items for systematic review and meta-analysis (PRISMA) guideline was used for literature search strategy, selection of publications, data extractions, and reporting result for the review process.ResultsA total of seven original research articles were included from a total of 35,999 research articles obtained from the different searching techniques. The selected articles were used, the same study design and laboratory methods to isolate different types of resistant bacteria. All studies isolate pathogenic bacteria and highlighted the presence of resistant bacteria for multiple antibiotics.ConclusionMultidrug resistance (MDR) bacteria were isolated from wastewater. This is an indication for the possible presence of pathogenic organisms that are discharged into the receiving environment probably waterbodies (lake and revers) posing risk to public health, animal, and environment. In Ethiopia the coverage of safe water supply is poor. This obligated the society to use untreated water from rivers, lakes and others. The outlet of most urban sewage from hospital, hotels, and industries are directly running to those water bodies due to lack of proper wastewater management system. Therefore ARB is a direct threat to those people that use those water bodies.

Assessment of Coliforms Bacteria Contamination in Lake Tanganyika as Bioindicators of Recreational and Drinking Water Quality

Worldwide coliform bacteria are used as indicators of environmental and fecal contamination and hence, the possible presence of pathogenic organisms. As most people living on the shores of Lake Tanganyika use its water for cooking, drinking and washing; the monitoring of organisms indicating water pollution is more predictive of the presence of certain pathogens to protect public health. This study was carried out along the Burundian coast at 4 sampling sites (Kajaga, Nyamugari, Rumonge and Mvugo) in the months of January, February and March 2018, to assess quantitatively the presence of coliform bacteria in comparison to the standards recommended by BIS-10500 (1991, 2012) and WWF-Pakistan (2007) for drinking and recreational water quality and to sensitize the populace using the untreated water about the potential health risks. The ColonyForming Unit (CFU) method was used and the results showed that total coliform bacteriaobtained was in the range of 9000 to 60000 CFU/100 mLand are indicative of environmental contamination of all sampling stations with an average of 33250 CFU/100 mL. Fecal coliform bacteria ranged from 0 to 5000 CFU/100 mL with an overall average of 2000 CFU/100 m Land Kajaga site appeared free of contamination as fecal coliform count there was nil. The Escherichia coli count recorded ranged from 0 to 3000 CFU/100 mL with an average of 1350 CFU/100 mL. At Kajaga stations, Escherichia coli count was 0 and therefore there is no evidence of recent fecal contamination. Thus, if only fecal contamination is taken into account, the water from Kajaga station can be considered as safe for drinking and bathing purposes but incidentally total coliforms were found at Kajagastation. The water from all sampling stations require treatment before any use.

Physicochemical and Bacteriological Quality of Public Swimming Pools in the Tamale Metropolis, Ghana

The study focused on the physicochemical and bacteriological quality of public swimming pools in the Tamale Metropolis. Physicochemical properties such as pH, temperature, and conductivity, and bacteria counts—including total coliform, faecal coliform, Escherichia coli (E. coli), Staphylococcus aureus, and total heterotrophic bacteria—were analyzed for their conformity with required health standards. The results obtained were analyzed using Student t test and compared with World Health Organization (WHO) and Environmental Protection Agency (EPA) standards for safe recreational and drinking water. The highest and the lowest temperatures were recorded in April (32.53 °C) and February (28.16 °C), respectively. The lowest and the highest mean pH values were 4.04 and 6.13, which were below acceptable standards. The conductivity level varied from 469.1563 µS cm−1 to 928.1563 µS cm−1. While the pH did not conform to acceptable standards, temperature and conductivity were within the EPA and/or the WHO acceptable limits. The total coliform (TC) expressed in colony-forming units per 100 mL ranged from 0 to 397 (cfu/100 mL), faecal coliform (FC) 0 to196 cfu/100 mL, E. coli 0 to 52 cfu/100 mL, Staphylococcus aureus (S. aureus) 8 to 27 cfu/100 mL, and Total Heterotrophic Bacteria (THB) 44 to 197 (cfu/mL). TC, FC, E. coli, S. aureus, and THB counts in most of the samples complied with the bacteriological standards. However, the bacterial loads increased and exceeded the WHO and/or EPA standards as the number of bathers increased. Besides, there were positive correlations between physicochemical parameters such as temperature, pH, and bacterial loads. Most parameters studied met the acceptable standards of recreational water stipulated by the WHO and EPA. However, the presence of pathogenic organisms in the recreational waters at any point in time should be treated as a public health concern, and hence a call for routine monitoring and inspection of public swimming pools in the Tamale Metropolis.

Efficacy and safety of quinolones for the treatment of uncomplicated urinary tract infections in women: a network meta-analysis.

Uncomplicated urinary tract infection (uUTI) is defined as the presence of pathogenic organisms in the urinary tract without anatomical and functional abnormalities, is accompanied by inflammatory leukocytes and cytokines and may or may not develop clinical symptoms. The frequency of uncomplicated urinary tract infection is higher in young women. Several quinolone treatment regimens are available; however, since we do not know which is the best antibiotic regimen for the treatment of this urinary infection, we analyzed the published evidence and conducted a systematic review with network meta-analysis. The aim was to compare and hierarchize quinolones according to their efficacy and safety and to identify the best treatment for uncomplicated urinary tract infection in women through a systematic review with network meta-analysis. Medline, Embase, LILACS, Cochrane CENTRAL and other databases were searched for trials. Bias in the trials was assessed using the Cochrane Collaboration tool. To analyze efficacy and adverse events, for direct comparisons, we obtained risk ratios and 95% confidence intervals by applying a fixed-effects model using tau2 and Q2 tests to calculate the heterogeneity. For the network meta-analysis, we analyzed the indirect comparisons by Bucher's method. We included 18 trials (8765 women). For premenopausal women, ofloxacin had a 57% probability of achieving remission but an 83% frequency of adverse events. For postmenopausal women, ofloxacin was 82% more effective for remission, with a 49% frequency of adverse events, compared with other types of quinolones. Compared with other quinolones, ofloxacin 200mg once daily for a treatment duration < 3days provides the highest clinical and bacteriological remission rates with the lowest relapse and resistance rates for the treatment of women with uUTIs. However, additional trials are needed to confirm our findings, especially when the treatment duration exceeds 3days.

Gerenciamento e caracterização dos recursos hídricos na comunidade do Barro Preto no município de Santa Maria de Itabira MG

Com a baixa disponibilidade de estudo sobre a situação dos recursos hídricos do povoado quilombola do Barro Preto, município de Santa Maria de Itabira MG, foram analisados em campo, parâmetros de qualidade da água, das questões do uso e ocupação do solo e algumas ações antrópicas do consumo ao descarte de esgoto no córrego do Barro Preto. Desta forma, este artigo visou analisar a gestão dos recursos hídricos da comunidade tanto para abastecimento quanto da qualidade da água do ribeirão. Para tanto, a pesquisa foi realizada de forma quali-quantitativa e descritiva, por meio de levantamentos realizados em campo sobre o uso e ocupação do solo na comunidade do Barro Preto. Assim, a observação e a pesquisa experimental foram utilizadas como coleta de dados, usando como unidade de análise o curso de água do manancial. Através da pesquisa em campo foram identificadas as formas desordenadas de uso e ocupação do solo, como a criação de gados em locais destinados a matas ciliares e a proximidade de casas ao ribeirão com descarte direto de esgoto bruto. As análises de água comprovaram a precariedade do manancial com interferências nas taxas de O², além da presença de organismos patogênicos como Escherichia Coli, que podem causar doenças à população consumidora da água do córrego do Barro Preto.

The Relationship Between Human Adenovirus and Metals and Semimetals in the Waters of the Rio Doce, Brazil.

The 2015 rupture of the Fundão dam near the district of Bento Rodrigues in Mariana, Minas Gerais, Brazil, released around 50 million m3 of iron ore tailings. The first tributary of the Rio Doceto receive this waste was the Gualaxo do Norte River. Many groups in Brazil and from around the world have studied the environmental and social impacts of this disaster. However, relationships between the introduction of mining waste, the geological complexity of the area, and the presence of pathogenic organisms have not yet been investigated. The present study aimed to measure the concentrations of enteric pathogens along the Gualaxo do Norte River after the environmental disaster and to correlate their abundance with the presence of metals and semimetals coming from both mining tailings and geological sources. For this purpose, we collected water samples from 27 stations along the entire basin during a hydrological year. The concentrations of metals and semimetals measured in this study were generally within limits established by national and international legislation, except for those of iron and manganese. Positive correlations between the human adenovirus (HAdV) and arsenic, barium, iron, lead, manganese, and nickel were confirmed, allowing us to observe that there is an abundance of the potentially infectious virus present in the studied sites containing metal/semimetal concentrations. These studies indicate the importance of investigations that consider viral enteric pathogens complexed with metals and may favor the stability and prolongation of the infectivity of such pathogens in water destined for human and animal usage.

Postoperative Pneumonia After Esophagectomy and Systemic Inflammatory Response Syndrome.

The aim of this study was to determine the association between post-esophagectomy pneumonia and the presence of pathogenic organisms in the sputum or pharynx and postoperative systemic inflammatory response syndrome (SIRS). This retrospective study included 98 patients diagnosed with esophageal cancer who had undergone esophagectomy. Postoperative pneumonia was observed in 24 patients (24.5%). Of the total 98 patients, 45 (45.9%) were tested positive for pathogenic organisms preoperatively, and 16 of those (35.6%) developed postoperative pneumonia; postoperative pneumonia occurred at a higher rate in these patients compared to pathogenic organism-negative patients (p=0.019). Postoperative SIRS was observed in 62 patients (63.3%), and 21 of these (33.9%) developed postoperative pneumonia, a significantly higher rate compared to patients without SIRS (p=0.007). Postoperative pneumonia was significantly associated with the presence of pathogenic organisms in the sputum or pharynx and postoperative SIRS.

Microbiological contamination in Smartphones

Smartphones tornaram-se um dos dispositivos essenciais mais utilizados para a comunicação diária. Raramente esses dispositivos são desinfetados, podendo ocorrer contaminação com vários agentes microbianos. Amostras coletadas nas telas touch screen de smartphones foram analisadas para determinar a contagem de bactérias aeróbias mesofílicas, enterobactérias, bolores e leveduras, bactérias coliformes totais e bactérias coliformes a 45 °C. Mais de 70% dos aparelhos analisados apresentaram contaminação elevada por bactérias mesófilas aerofílicas e bolores e leveduras, além de 25% por enterobactérias, sendo observada a presença de coliformes a 45 °C em 25% dos aparelhos avaliados. A elevada contaminação por mesófilos aeróbios e bolores e leveduras e a presença de enterobactérias e bactérias coliformes a 45 °C sugerem um risco à saúde dos usuários devido à possível presença de organismos patogênicos.

Metagenomic analysis of the bacterial communities and their functional profiles in water and sediments of the Apies River, South Africa, as a function of land use

Water quality is an important public health issue given that the presence of pathogenic organisms in such waters can adversely affect human and animal health. Despite the numerous studies conducted to assess the quality of environmental waters in many countries, limited efforts have been put on investigating the microbial quality of the sediments in developing countries and how this relates to different land uses. The present study evaluated the bacterial diversity in water and sediments in a highly used South African river to find out how the different land uses influenced the bacterial diversity, and to verify the human diseases functional classes of the bacterial populations. Samples were collected on river stretches influenced by an informal, a peri-urban and a rural settlement. Genomic DNA was extracted from water and sediment samples and sequenced on an Illumina® MiSeq platform targeting the 16S rRNA gene variable region V3-V4 from the genomic DNA. Metagenomic data analysis revealed that there was a great diversity in the microbial populations associated with the different land uses, with the informal settlement having the most considerable influence on the bacterial diversity in the water and sediments of the Apies River. The Proteobacteria (69.8%), Cyanobacteria (4.3%), Bacteroidetes (2.7%), and Actinobacteria (2.7%) were the most abundant phyla; the Alphaproteobacteria, Betaproteobacteria and Anaerolineae were the most recorded classes. Also, the sediments had a greater diversity and abundance in bacterial population than the water column. The functional profiles of the bacterial populations revealed an association with many human diseases including cancer pathways. Further studies that would isolate these potentially pathogenic organisms in the aquatic environment are therefore needed as this would help in protecting the lives of communities using such rivers, especially against emerging bacterial pathogens.

Assessment of Water Quality and Microbial Load of Dhaleshwari River Tangail, Bangladesh

The present study was undertaken to investigate some water quality parameters (pH, BOD, DO and water temperature), anion concentration (PO43- , SO42- and NO3- ) and the availability of some selected microorganisms consisted of total coliform, fecal coliform (Escherichia coli) and Vibrio cholerae in the Dhaleshwari river during the period from April to June 2015. The water samples were collected from three different stations of the river namely Belta (St-1), Kendua (St-2) and Charabari (St-3) under the Porabari union at Tangail Sadar Upazilla. Water temperature varied from 29.5 oC in June to 31.7 oC in May with a highest mean 31.17 ± 0.47 in May. The pH of water was slightly alkaline in nature and ranged between 7.25 in June and 8.45 in April. Mean concentrations of BOD 0.87 ± 0.19, 1.12 ± 0.6 and 0.97 ± 0.67 were recorded in April, May and June respectively. Highest PO42- value 4.4 mg/l was found in May and lowest value of PO42- 1.74 mg/l was found in June. Mean sulfate values at different months of Dhaleshwaririver were 6.61 ± 1.78 mg/l, 7.61 ± 2.16 mg/l and 3.68 ± 0.23 mg/l respectively. Nitrate concentration in water samples varied from 0.51 mg/l in June to 3.66 mg/l in April. Total coliforms bacteria were found high in all water samples and values ranged from 11.05 × 108 - 19 × 1023 cfu/ml. The highest load of total coliforms bacteria was recorded at St-2 in May and the lowest was at St-1 in April. The highest concentration of (Escherichia coli counted in water sample was 6 × 1013 cfu/ml at St-2 during June, while lowest concentration was 5 × 105 cfu/ml at St-1 during May. The highest concentration of Vibrio cholerae was 9.66 × 1014 cfu/ml at St-1 during June, while lowest concentration was 4.2 × 108 cfu/ml at St-3 during April. The high number of total coliform, Escherichia coli and Vibrio cholerae indicated significant level of microbial pollution in Dhaleshwari river. The presence of pathogenic organisms may pose not only health hazards to the users of the river water but also poses risks for the aquatic organisms especially fish of the water bodies. So the occurrence of high bacterial load concluded that the water of Dhaleshwari river is polluted and unhygienic for drinking and even unsuitable for domestic purposes without proper treatment.

Soil and Water Microbiological Characteristics of Olo Flowstation and Rumuekpe Metering Station, Rivers State, Nigeria

This study evaluates the soil and microbiological properties of Olo Flowstation and Rumuekpe Metering Station. Standard sampling and analytical methods were adopted. Results of the soil microbiological composite samples from the random samples showed that the percent Hydrocarbon Utilizing Bacteria (HUB) was 40.0% and 27.27% for the top and subsoil, respectively. Though these values were high, they were below expected values in view of the high level of petroleum hydrocarbon spilled on the soils collected. The moderately high amount of hydrocarbon degraders in these soils compared to the control indicated that these soils might have been in contact with petroleum products which might have polluted the soils. The poor drainage resulting from stagnant water might have leached the petroleum products down the soil along transect, resulting into higher HUB% in the subsoil than the top soil. Other sampling stations had better top soil drainage hence lower % HUB down the profile. All the water samples, both surface and groundwater showed significant range of bacteria. The colonies were within 30 to 300. The growth for bacteria ranges from 1.5 × 105 cfu/ml at Elele-Alimini (up-stream) to 3.0 × 105 cfu/ml at the waste pit, Sombreiro River and flare pit. The heterotrophic organisms isolated were Bacillins sp., Chromobacterium sp., Micrococcus sp, Enterobacter sp., and Psendomonas sp. The presence of the bacteria in the water bodies (effluent) indicated a regrowth and general bacterial composition of the water. The petroleum degraders isolated in the surface waters where Chromobacterium sp., Micrococcus sp., Psendomonas sp. and Bacillus sp. The microorganisms population range from 1.0 × 102 at Elele-Alimini down-stream to 6.0 × 102 at discharge point-Waste pit and the borrow pit at Rumuekpe. The preponderance of petroleum utilizing bacteria in these water samples indicated the presence of hydrocarbons. The fungal count was generally low and was found in Elele-Alimini up-stream and borrow pit near the metering station at Rumuekpe. The mean count of E. coli per 100 ml of water was 180+ meaning that all the water samples both surface and groundwater were polluted. The presence of E. coli showed faecal pollution of the water and there could be possible presence of pathogenic organisms in the water. This study therefore will be a useful guide to farmers in the areas around the flow stations. There is also need for treatment of surface and groundwater in the area. It is recommended that regular monitoring of the geo-environmental media be carried out.

Insights into Soluble Toll-Like Receptor 2 as a Downregulator of Virally Induced Inflammation.

The ability to distinguish pathogens from self-antigens is one of the most important functions of the immune system. However, this simple self versus non-self assignment belies the complexity of the immune response to threats. Immune responses vary widely and appropriately according to a spectrum of threats and only recently have the mechanisms for controlling this highly textured process emerged. A primary mechanism by which this controlled decision-making process is achieved is via Toll-like receptor (TLR) signaling and the subsequent activation of the immune response coincident with the presence of pathogenic organisms or antigens, including lipid mediators. While immune activation is important, the appropriate regulation of such responses is also critical. Recent findings indicate a parallel pathway by which responses to both viral and bacterial infections is controlled via the secretion of soluble TLR2 (sTLR2). sTLR2 is able to bind a wide range of pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs). sTLR2 has been detected in many bodily fluids and is thus ubiquitous in sites of pathogen appearance. Interestingly, growing evidence suggests that sTLR2 functions to sequester PAMPs and DAMPs to avoid immune activation via detection of cellular-expressed TLRs. This immune regulatory function would serve to reduce the expression of the molecules required for cellular entry, and the recruitment of target cells following infection with bacteria and viruses. This review provides an overview of sTLR2 and the research regarding the mechanisms of its immune regulatory properties. Furthermore, the role of this molecule in regulating immune activation in the context of HIV infection via sTLR2 in breast milk provides actionable insights into therapeutic targets across a variety of infectious and inflammatory states.

Examination of Faecal Caliform Presence in Groundwater in Ghana (A Case Study in Ketu South Municipality)

Samples were taken from wide-dam, open wells and compared with piped city water. Well water from both districts showed widespread contamination with faecal bacteria. This study revealed high Faecal and Total coliforms in all the samples with varying numbers. The high coliforms content of the eight hand-dug wells poses a health risk and renders the water unsuitable for human consumption. The presence of pathogenic organisms in the water could be attributed to pit latrine in the vicinity that extent their influence on these water qualities, since they are sited close to them. © JASEM