Lynx Presence Research Articles

Evidence for a survival-driven traveling wave in a keystone boreal predator population

Cyclical population dynamics are a common phenomenon in populations worldwide, yet the spatial organization of these cycles remains poorly understood. In this study, we investigated the spatial form and timing of a population collapse from 2018 to 2022 in Canada lynx (Lynx canadensis) across the northwest boreal forest. We analyzed survival, reproduction, and dispersal data from 143 individual global positioning system (GPS) collared lynx from populations across five study sites spanning interior Alaska to determine whether lynx displayed characteristics of a population wave following a concurrent wave in snowshoe hare (Lepus americanus) abundance. Reproductive rates declined across the study sites; however, site-level reproduction declined first in our easternmost study sites, supporting the idea of a population wave. Despite a clear increase in percent of dispersing lynx, there was no evidence of directional bias in dispersal following a hare population wave. Analysis did show increasingly poor survival for lynx dispersing to the east compared to combined resident and westward dispersal. This pattern is consistent with a survival-mediated population wave in lynx as the driver of the theorized population wave. The combination of these factors supports the idea of a hierarchical response to snowshoe hare population declines with a drop in lynx reproduction followed by increased dispersal, and finally reduced survival. All of this evidence is consistent with the expected characteristics of a population undergoing a traveling wave and supports the hypothesis that lynx presence may facilitate and mirror the underlying wave patterns in snowshoe hare.

Apex predators can structure ecosystems through trophic cascades: Linking the frugivorous behaviour and seed dispersal patterns of mesocarnivores

Abstract Current global change scenarios demand knowledge on how anthropogenic impacts affect ecosystem functioning through changes in food web structure. Frugivorous mesocarnivores are a key link in trophic cascades because, while their abundance and behaviour are usually controlled by apex predators, they can provide high‐quality seed‐dispersal services to plant communities. Thus, the recent rewilding of large carnivores worldwide can trigger cascading effects for plants. We investigated the top‐down effects of an apex predator (Iberian lynx Lynx pardinus) on seed‐dispersal services mediated by two mesocarnivore species (red fox Vulpes vulpes and stone marten Martes foina) at the plant community level by comparing areas with and without lynx in a Mediterranean mountain range in Southern Spain. We collected scats of mesocarnivores (n = 1575) to assess frugivory and seed dispersal of 15 plant species over two consecutive fruiting seasons and two habitat types (open and forest). Specifically, we assessed the effect of lynx presence on (i) seed occurrence and fleshy‐fruit biomass per scat, (ii) number of scats containing seeds and (iii) diversity of dispersed seeds. The quantity and diversity of dispersed seeds drastically decreased under predation risk for both mesocarnivore's species. Seed dispersal by stone martens was negatively affected by the presence of lynx, with a marked reduction in the number of scats with seeds (93%) and the diversity of dispersed seeds (46%). Foxes dispersed 68% fewer seeds in open habitats when coexisting with lynx, probably leading to differential contributions to seed‐dispersal effectiveness among habitats. Our study reveals a novel trophic cascade from apex predators to plant communities. The behavioural responses of frugivorous mesocarnivores to predation risk and the reduction in the intensity of their faecal deposition pattern are probably related to their lower abundance when co‐occurring with apex predators. While rewilding apex predators is a successful conservation tool, attention should be paid to cascading effects across food webs, particularly where frugivore megafauna is missing and mesocarnivores provide unique services to plants. Read the free Plain Language Summary for this article on the Journal blog.

Revealing the Prevalence of Toxoplasma in Sierra Morena's Wild Boar: An ELISA-Based Study Using Meat Juice.

This research work focused on the prevalence of Toxoplasma gondii in wild boar from the Sierra Morena region. We conducted an ELISA analysis using meat juice samples. A total of 892 samples from six hunting seasons (2013-2019) were collected from the provinces that constitute the Sierra Morena Mountain range. These samples were analyzed using the Pigtype® ELISA kit, specifically developed for detecting T. gondii in meat juice. The overall prevalence of T. gondii in Sierra Morena was 23.2%. The highest prevalences were observed in Córdoba (31.6%) and Jaén (25.9%). These provinces exhibit the highest density of wild boar as well as the greatest presence of the Iberian lynx (Lynx pardinus). Further in-depth studies are necessary, but it appears that the presence of wild felids and scavenger behavior may be associated with this observation.

Abundance of Eurasian lynx in the mountain taiga of the Southern Urals

The study aims to analyze the dynamics of the Eurasian lynx in the mountain taiga, and to search for the factors affecting the numbers of this predator. The study was conducted in the Southern Urals (Russia) from 2008 to 2019. The dynamics of lynx and its main prey numbers Capreolus pygargus, Lepus timidus and L. europaeus, Tetrao urogallus and Bonasa bonasia is based on the data obtained during the complex winter census (CWC). Annual CWCs were performed in Chelyabinsk Oblast with total area of 23.445.5 km². The hypotheses on the influence of snow depth on the number of lynx and the relationship between the dynamics of the predator and prey abundances were tested using regression method. The relationship between the number of lynx and snow depth were obtained using Spearman's coefficient. The number of lynxes varied from 51 to 281 individuals, population density - from 0.09 lynx/100 km2 to 0.48 lynx/100 km2 . The lowest abundances were recorded in 2013 and 2016. In the winters of 2013 and 2016, the snow depth was 2.0-2.5 times greater than on average for long-term observations. However, no significant decrease in the number of the Siberian roe deer was observed in these years, while the number of lynxes decreased by 2 times compared to the previous years. No significant correlation was found between the abundance of lynxes and the Siberian roe deer, r = 0.25 ± 0.3. A negative relationship was found between the amount of precipitation and the winter number of lynxes (r = -0.717 ± 0.22; p ˂ 0.05). The amount of snow cover in the Southern Ural Mountains is an important factor affecting the distribution of ungulates. The snow depth may determine the lynx presence both directly and through the changes in the number of its main prey.

Leading the way – presence of brown bear <i>Ursus arctos</i>, lynx <i>Lynx lynx</i> and grey wolf <i>Canis lupus</i> underlines the integrity and corridor function of the upper Neretva Valley in Bosnia and Herzegovina

Despite its connecting character to the Sutjeska National Park in the North-East, the course of the upper Neretva (Ulog and Borač valley) in Bosnia and Herzegovina (BiH), has to date not been an area of focus for carnivore research. With the aim of contributing to an improved understanding of the present distribution of large and endangered carnivores in this area, a field survey was conducted during the Neretva Science Week 2022. Opportunistic data collection was carried out along a predefined road transect (20 km) and six line transects (13 km) tracked by foot. Additionally, reports of mammal signs for six corresponding research sites along the Neretva were registered. Altogether, at least 15 mammal species could be distinguished. From the identified carnivores, four are listed in Annex II of the Habitats Directive. Furthermore, the signs of lynx presence, in addition to brown bear and grey wolf, highlight the connectivity value of the upper Neretva in its current state. Based on information from local practitioners, the main threats to large carnivores are hunting pressure and a negative perception as competitors. A likely decline in habitat quality and fragmentation, due to the planned hydropower plants expansion and logging activities, exacerbate these threats. Consequently, further systematic monitoring is recommended to assess the possible impact of human driven environmental changes.

Public acceptance of Eurasian lynx (Lynx lynx) in Germany

In a geological epoch, referred to as the Anthropocene, where large carnivores are increasing and expanding across Europe simultaneously to human activity impacting wildlife population numbers, an understanding of how to manage conservation success is required. Eurasian lynx (Lynx lynx) populations have expanded back into some of their former ranges within Germany through natural re-establishment and reintroductions. Several proven illegal killings of Eurasian lynx (hereafter lynx) in Germany question the acceptance of lynx within one of its former ranges. An online self-administered questionnaire (n = 1195) was distributed across Germany collecting data on demographic factors, knowledge of lynx ecology, feelings on the presence of lynx, and future management of lynx populations. The questionnaire data was analysed through non-parametric tests. The results suggested that acceptance of lynx was similarly high across German states. No significant differences were found in acceptance scores between lynx absence or presence, nor within areas of different lynx re-establishment processes. Age, education, profession, and awareness or experience of lynx attacks were significantly associated with acceptance scores. Longitudinal monitoring would prove beneficial in assessing acceptance levels of this large carnivore amongst the public in Germany. Acceptance of lynx assists in creating a balanced ecosystem where large carnivores and humans can co-exist and share the same landscape.

Potencjalnie ważne terytoria występowania rysia euroazjatyckiego (Lynx lynx) jako podstawa do wyznaczenia elementów strukturalnych ekosieci regionu Zakarpacia na Ukrainie

The purpose of the publication is to indicate potentially important areas for the presence of the Eurasian lynx (Lynx lynx) within the Transcarpathian region in Ukraine using GIS modeling tools to optimize the network of protected areas. The modeling consisted of a preliminary analysis of the following five parameters that are important for the life of the species: type of land cover, proximity of non-forest biotopes to forest biotopes, high-altitude bioclimatic zones, degrees of relief dissection, distance from settlements and roads. A comprehensive GIS analysis of these parameters made it possible to establish that the total area of territories that are potentially suitable for the presence of the Eurasian lynx within the Transcarpathian region is 192.6 thousand hectares, and covers about 9.9% of the territory of the region. The most suitable areas are concentrated in the mountainous southeastern part of the region within the orographic ecoregion of the elevated dissected highlands. Within the modeled habitats, there are often places where real signs of the species’ distribution have been recorded. In the second stage of the research, a cartographic model “Territories of the potential presence of the Eurasian lynx (Lynx lynx) and key areas of the regional ecological network of the Transcarpathian region” was obtained, where population, reproduction and other areas, as well as the degree of nature protection in these areas, were established.

Predation risk can modify the foraging behaviour of frugivorous carnivores: Implications of rewilding apex predators for plant-animal mutualisms.

Apex predators play key roles in food webs and their recovery can trigger trophic cascades in some ecosystems. Intra‐guild competition can reduce the abundances of smaller predators and perceived predation risk can alter their foraging behaviour thereby limiting seed dispersal by frugivorous carnivores. However, little is known about how plant–frugivore mutualisms could be disturbed in the presence of larger predators.We evaluated the top‐down effect of the regional superpredator, the Iberian lynx Lynx pardinus, on the number of visits and fruits consumed by medium‐sized frugivorous carnivores, as well as the foraging behaviour of identified individuals, by examining the consumption likelihood and the foraging time.We carried out a field experiment in which we placed Iberian pear Pyrus bourgaeana fruits beneath fruiting trees and monitored pear removal by frugivorous carnivores, both inside and outside lynx ranges. Using camera traps, we recorded the presence of the red fox Vulpes vulpes, the Eurasian badger Meles meles and the stone marten Martes foina, as well as the number of fruits they consumed and their time spent foraging.Red fox was the most frequent fruit consumer carnivore. We found there were fewer visits and less fruit consumed by foxes inside lynx ranges, but lynx presence did not seem to affect badgers. We did not observe any stone marten visits inside lynx territories. The foraging behaviour of red foxes was also altered inside lynx ranges whereby foxes were less efficient, consuming less fruit per unit of time and having shorter visits. Local availability of fruit resources, forest coverage and individual personality also were important variables to understand visitation and foraging in a landscape of fear.Our results show a potential trophic cascade from apex predators to primary producers. The presence of lynx can reduce frugivorous carnivore numbers and induce shifts in their feeding behaviour that may modify the seed dispersal patterns with likely consequences for the demography of many fleshy‐fruited plant species. We conclude that knowledge of the ecological interactions making up trophic webs is an asset to design effective conservation strategies, particularly in rewilding programs.

Rasprostranjenost i najmanja veličina populacije euroazijskog risa (Lynx lynx) u Hrvatskoj u razdoblju 2018.–2020.

Scientific data on distribution and abundance of endangered species are the foundation for their effective conservation and management. In this paper, we present results of the first scientifically – based estimation of lynx population size in Croatia. The goal of the study was to determine the area of lynx distribution and to estimate the minimum size of lynx population in Croatia in the period 2018 - 2020. To determine lynx distribution, 902 signs of lynx presence were collected in the period from the beginning of May 2018 until the end of April 2020. Out of those, 92.8% of lynx observations were categorized as C1, 2.8% as C2 and 4.4% as C3. Permanent lynx presence was confirmed in Primorsko – Goranska and Ličko – Senjska county, in southern part of Karlovac county and north-eastern part of Zadar county on the total surface of 7200 km2. For the minimum population size estimation, 804 camera trap photographs led to identification of 89 – 108 adult lynxes. Among 108 identified individuals there were 29 females, 22 males, while for 7 animals the sex was not determined. During the two reproductive seasons, we photographed 44 cubs in 25 litters. Future important steps in lynx population monitoring are correcting the deficiencies identified in this study and implementation of methodology that will allow us to use spatial capture recapture models for estimation of lynx abundance in Croatia.

Comparison of methods for estimating omnidirectional landscape connectivity

Maintaining and improving ecological connectivity is an important component of wildlife conservation. Omnidirectional circuit theory algorithms model the flow of electric current across a resistance grid from all directions, making them particularly useful for modeling connectivity of multiple or widespread species, or when source and destination sites are not specified, such as in the case of continuously distributed species. We compared three published omnidirectional connectivity methods—the point-based method, the wall-to-wall method, and the Omniscape method. We compared the three omnidirectional connectivity methods using ten simulated resistance grids, representing commonly encountered landscape features. We then used three Canada lynx (Lynx canadensis) snow-track datasets from Ontario, Canada to validate the ability of each method to detect landscape use. Current density maps generated by all three methods were highly correlated for most simulated landscapes, with the exception of uniform resistance grids. In uniform grids, without landscape features to guide current, each method revealed unique node-placement biases. All three methods produced similar connectivity maps for lynx, showing higher current at lynx presence locations compared to absence locations. The main differences between methods were not in their output, but in their implementation and use of computational resources. Our findings suggest that all three currently published omnidirectional connectivity models produce similar current density maps and can accurately map landscape connectivity. We provide guidelines for selecting a method based on factors such as computational resources, study area, and project goals.

Camera-Trapping Two Felid Species: Monitoring Eurasian Lynx (Lynx lynx) and Wildcat (Felis silvestris) Populations in Mixed Temperate Forest Ecosystems

Documenting reliable and concrete data makes camera-trapping an appropriate tool for many secretive large mammals. Camera-trapping has been widely used for detecting the large mammal fauna of Turkey. However, systematic surveys and comprehensive assessments are still not sufficient for assessing wildlife populations in the country. In the present study, populations of Eurasian lynx (Lynx lynx) and wildcat (Felis silvestris) were monitored by systematic camera-trapping, and their co-existence was investigated with 70 camera-trap stations between 2014–2018 in northern Anatolia. Daily activity patterns and kernel density estimation were used to analyse and compare temporal patterns of target species. High temporal overlap (Δ4: 0.81) for Eurasian lynx and wildcat at the same study sites, and no alteration in wildcat activity pattern in the presence of Eurasian lynx, suggest that these two felid species can co-exist in the same habitats using the same period of the day. Domestic cat (Felis catus) was detected at 52.5% of the wildcat-positive stations indicating that there is hybridisation threat for the wildcat population in the region. The study reveals that systematic camera-trapping surveys are an applicable method for monitoring cryptic mammalian carnivores and provide useful insights into the threats on wildlife populations like hybridisation.

Planning the peninsula-wide recovery of the Iberian lynx: identification of favourable habitat areas

Abstract The creation of new populations through reintroductions in their former range is still necessary for the long-term conservation of the Iberian lynx (Lynx pardinus). A large-scale assessment of habitat suitability is a necessary prerequisite for evaluating the possibility of carrying out lynx reintroductions. We modelled habitat suitability for the Iberian lynx in the Iberian Peninsula using MaxEnt. Lynx presence data for 2010–2013 from Andujar-Cardeña and Doñana populations were used. Habitat variables were quantified using the CORINE Land Cover. This habitat suitability model predicted an environmentally suitable area of 87,747 km2, which represents 14.08% of the whole Iberian Peninsula. Of the total suitable habitat identified, 45% is located in a large continuous area in the south-western quadrant coinciding with the historical range of the species in the mid-20th century. However, there are also relevant patches mainly in the north-eastern quadrant. About 55% of the potential area suitable for the lynx falls outside protected areas. If Iberian lynx populations are to attain levels that will facilitate their long-term survival, it will be necessary to consider potential habitat for the species throughout the whole of the Iberian Peninsula.

Valuation of Large Carnivores and Regulated Carnivore Hunting

Large carnivores are keystone species but represent economic costs to hunters. In Sweden, carnivore territories generally overlap with hunting areas, and as a result, conflicts occur because of the competition for prey. The wolf, lynx, and brown bear are protected species by law but are hunted when authorities allocate license hunting quotas. The aim of these quotas is to limit carnivore numbers. We estimate a hedonic model using ordinary least squares to address the effect of large carnivore occurrence on hunting lease prices by accounting for the presence of license hunting quotas for predators. This result is compared with the least absolute deviation estimations, which reduce the influence of outliers in the survey data. To isolate the effect of carnivores on hunting lease prices, we use snow depth and forest productivity as proxy variables for game harvest in the absence of carnivores. Our results show that lynx and wolf presence reduce hunting lease prices, but lynx presence shows higher significance and robustness. Based on median regressions, the marginal implicit price of an additional wolf territory is about 15% larger than that of an additional lynx territory. In contrast, we found no conclusive evidence that bear abundance directly affects hunting lease prices, but regulated bear hunting is found to have a positive and significant impact on hunting leases, suggesting indirect positive net benefits of increased brown bear abundance.

Fear of the dark? Contrasting impacts of humans versus lynx on diel activity of roe deer across Europe.

Humans, as super predators, can have strong effects on wildlife behaviour, including profound modifications of diel activity patterns. Subsequent to the return of large carnivores to human-modified ecosystems, many prey species have adjusted their spatial behaviour to the contrasting landscapes of fear generated by both their natural predators and anthropogenic pressures. The effects of predation risk on temporal shifts in diel activity of prey, however, remain largely unexplored in human-dominated landscapes. We investigated the influence of the density of lynx Lynx lynx, a nocturnal predator, on the diel activity patterns of their main prey, the roe deer Capreolus capreolus, across a gradient of human disturbance and hunting at the European scale. Based on 11 million activity records from 431 individually GPS-monitored roe deer in 12 populations within the EURODEER network (http://eurodeer.org), we investigated how lynx predation risk in combination with both lethal and non-lethal human activities affected the diurnality of deer. We demonstrated marked plasticity in roe deer diel activity patterns in response to spatio-temporal variations in risk, mostly due to human activities. In particular, roe deer decreased their level of diurnality by a factor of 1.37 when the background level of general human disturbance was high. Hunting exacerbated this effect, as during the hunting season deer switched most of their activity to night-time and, to a lesser extent, to dawn, although this pattern varied noticeably in relation to lynx density. Indeed, in the presence of lynx, their main natural predator, roe deer were relatively more diurnal. Overall, our results revealed a strong influence of human activities and the presence of lynx on diel shifts in roe deer activity. In the context of the recovery of large carnivores across Europe, we provide important insights about the effects of predators on the behavioural responses of their prey in human-dominated ecosystems. Modifications in the temporal partitioning of ungulate activity as a response to human activities may facilitate human-wildlife coexistence, but likely also have knock-on effects for predator-prey interactions, with cascading effects on ecosystem functioning.

Using MaxEnt algorithm to assess habitat suitability of a potential Iberian lynx population in central Iberian Peninsula

Iberian lynx distribution is currently restricted to the south of the Iberian Peninsula. Nevertheless, there is evidence of the presence of several small groups in the peninsular centre that have been forgotten by management and conservation actions. In this research, we gathered evidences of Iberian lynx presence along 21 transects located in the southwest of the Madrid province. In these transects lynx DNA was identified in 47 scats, which scientifically proves the presence of the species in that location. Using these locations (presence-only data) we built a maximum entropy model (MaxEnt) to estimate the suitability of the study area for the species. Our results show the existence of an almost continuous area that is approximately 744 km2 that is suitable for the Iberian lynx. Seventy-eight percent of this area is within the Natura 2000 network and, therefore, it falls under regulations to preserve and restore habitat types, flora and fauna. This study shows the suitability of this territory has for the Iberian lynx.

Spatial segregation between Iberian lynx and other carnivores

Rabbits represent the bulk of its diet as for many other Iberian predators. This study addresses how the presence of the Iberian lynx affects the spatial distribution of the mesocarnivore community at landscape scale in the Sierra de Andújar. We studied mesocarnivore presence by sampling at 230 camera trapping stations, located in areas with and without lynx. We used a x2–test to compare the proportion of stations in which each species of carnivore were recorded in the zones with and without lynx. The proportion of camera trapping stations in which red fox (Vulpes vulpes), Egyptian mongoose (Herpestes ichneumon), beech marten (Martes foina), wildcat (Felis sylvestris) and common genet (Genetta genetta) were detected was significantly lower in the area where lynx were present than in the area where it was absent. No significant differences between the two types of areas were found for badgers (Meles meles). Our results highlight the role of the lynx as apex predators and the benefits that the recovery of Iberian lynx populations would entail in terms of trophic interactions and restored disrupted ecosystems processes.Key words: Intraguild competition, Carnivores, Phototrapping, Apex preda

Positive Beispiele der Wald-Wild-Situation bei der Weisstannenverjüngung

Positive examples of forest-ungulate situations focusing on silver fir regeneration Roe deer, chamois and red deer browse on the apical shoots of young trees, and particularly selected are those of the European silver fir. Because of this selective feeding of ungulates, the silver fir is unable to sustain its population density relative to other tree species. However, due to locally functioning forest-ungulate situations, there have been areas of improved silver fir regeneration during the last few years. In this study, twenty different sites exhibiting such improvement have been analysed. Eighteen sites were situated in thirteen different Swiss cantons, as well as one each in Germany and Austria. With the aid of interviews with experts such as local foresters, gamekeepers, hunters and representatives of the cantonal departments of hunting and forestry, the importance of sixty-six potential influencing factors on a locally improved forest-ungulate system has been investigated. Frequency analysis has shown that the density of roe deer, the availability of light and the presence of lynx are the most important of these factors. But also the density of chamois, the increased focus on a natural regeneration and the form of management have been frequently mentioned as central factors. Changes in communication between the hunting and forestry sides have been considered most often as central or important. Positive examples could be examined in all regions of Switzerland – in cantons with patent hunting and hunting districts as well as in areas with different densities and compositions of wild ungulates. It does not seem that a single influencing factor, but rather a combination of different factors was decisive for improved silver fir regeneration.

The importance of lagomorphs for the Eurasian lynx in Western Asia: Results from a large scale camera-trapping survey in Turkey

Explaining predator-prey relationship is crucial for implementing effective conservation practices on large mammals. The Eurasian lynx (Lynx lynx) preys on small ungulates such as roe deer (Capreolus capreolus) in Europe, and on hares (Lepus spp.) in more eastern longitudes, but there is lack of information on the southernmost populations of the Eurasian lynx in Turkey.This study explores the spatial and temporal relationship of the Eurasian lynx with its two main preys, roe deer and the brown hare (Lepus europaeus) in north-western Anatolia using long-term camera-trapping data. Camera-trapping surveys were held with 173 systematic camera-trap stations at 10 study sites between November 2007 and July 2016, and reached to 53,995 trap-nights in total. To analyse the camera-trap data, we used a generalized linear mixed model (GLMM) assuming binomial error distribution for presence/absence data and general linear mixed model (LMM) for the relative abundance data. In both modelling approaches, we considered the study site as the random factor. We estimated the overlap of daily activity patterns of Eurasian lynx with roe deer and brown hare using kernel density estimation.Both GLMM and LMM analyses indicated a significant spatial relationship between the Eurasian lynx and the brown hare (χ2 = 22.4, P < 0.0001 and Likelihood ratio = 4.8, P < 0.05, respectively), but not between the Eurasian lynx and roe deer (P > 0.05). The daily activity of Eurasian lynx highly overlapped with brown hare (Δ4 = 0.81), but we found a lower overlap between Eurasian lynx and roe deer (Δ4 = 0.62). The findings reveal that the presence of Eurasian lynx is temporally and spatially synchronized with the brown hare in northern Anatolia, and support that the brown hare, but not the roe deer, constitutes the main diet of Eurasian lynx in the study area.

New old news on the “Lobo cerval” (Lynx lynx?) in NE Spain

There is growing evidence that two Lynx species were present in Spain until recent times: the Iberian lynx (Lynx pardinus) in Mediterranean areas and the Eurasian lynx (Lynx lynx) in the Atlantic and Alpine ones. The recent presence of the Eurasian lynx is mainly deduced from stories and news about fierce animals known as “lobos cervales” or “tigres”, these being dangerous beasts for livestock, a condition that is difficult to associate with the Iberian lynx. In this work, we provide new records of lynx in NE Iberian Peninsula that blur the geographic boundaries between both species. The contact between the Iberian and Eurasian lynxes could explain the signs of hybridization detected between them.

High prevalence of Lynx rufus gammaherpesvirus 1 in wild Vermont bobcats.

Gammaherpesviruses (GHVs) are host specific DNA viruses that infect a large range of mammalian species. These viruses preferentially target host lymphocyte cell populations and infection may lead to morbidity or mortality in immunocompromised, co-infected, or non-adapted hosts. In this study, we tested for the presence of Lynx rufus gammaherpesvirus 1 (LruGHV1) in a northeastern United States population of wild bobcats (L. rufus). We estimated prevalence of infection and viral load in infected individuals using quantitative real-time PCR analysis of spleen DNA from 64 Vermont bobcats. We observed an overall prevalence of 64% using this methodology. Bobcat age was significantly positively associated with GHV infection status, and we noted a trend for higher viral loads in young animals, but prevalence and viral load were similar in male and female bobcats. A single LruGHV1 variant was identified from the sequencing of the viral glycoprotein B gene of Vermont bobcats. This gene sequence was 100% similar to that reported in Florida bobcats and slightly variant from other isolates identified in the Western USA. Our work suggests broad geographic distribution and high prevalence of LruGHV1 in bobcat populations across the United States with infection attributes that suggest horizontal transmission of the agent. Geographic differences in viral genotype may reflect historical migration and expansion events among bobcat populations.