Presence Of Extracellular Enzymes Research Articles

Studies On the Role of Fungal Strains in Bioremediation of Dyes Isolated from Textile Effluents

Fungal strains are widely used for the cleaning of soil, sediments, groundwater, surface water, and the ecosystem. The presence of extracellular enzymes in fungi facilitates the process of bioremediation of textile dyes. This study was conducted to observe the quality of water being released from textile dyes industries and also the capability of some fungal strains which can remediate these dyes by showing the tendency of their resistance. Samples of water were collected from the polluted area surrounding the textile dyeing industries in Lahore. In the process of isolation, Potato Dextrose Agar (PDA) medium was used to verify the fungal growth. Fungal strains were purified, and the morphological characterization of fungal strains was carried out at 10X and 100X by using a magnification microscope. The fungal strains, such as Aspergillus niger, Aspergillusoryzae, and Aspergillusflavus were identified. The stress of four types of dyes was given to each fungal strain. The results showed that Aspergillusoryzae was one of the most stable, non-toxic, and resistant fungal species against the high stress of dyes as compared to other species

Bioactives and Extracellular Enzymes Obtained from Fermented Macrofungi Cultivated in Cotton and Jatropha Seed Cakes.

This work focused on obtaining fermented oil cake (cotton or Jatropha) via macrofungi growth with potential characteristics for animal feed formulations, such as the presence of extracellular enzymes, bioactive (ergosterol and antioxidants), and detoxification of antinutritional compounds. The concentration of phorbol esters was reduced by four macrofungi in Jatropha seed cake (JSC) to non-toxic levels. At least two macrofungi efficiently degraded free gossypol in cottonseed cake (CSC). Fermentation with Coriolopsis sp. INPA1646 and Tyromyces sp. INPA1696 resulted in increased ergosterol concentrations, antioxidant activity reduction, and high activity of laccases and proteases. Bromatological analysis indicated high crude protein concentrations, with partial solubilization by fungal proteases. Fermented products from Coriolopsis sp. and Tyromyces sp. in JSC or CSC can be considered important biological inputs for monogastric and polygastric animal feed.

Studies On the Role of Fungal Strains in Bioremediation of Dyes Isolated from Textile Effluents

Fungal strains are widely used for the cleaning of soil, sediments, groundwater, surface water, and theecosystem. The presence of extracellular enzymes in fungi facilitates the process of bioremediation of textile dyes. Thisstudy was conducted to observe the quality of water being released from textile dyes industries and also the capabilityof some fungal strains which can remediate these dyes by showing the tendency of their resistance. Samples of waterwere collected from the polluted area surrounding the textile dyeing industries in Lahore. In the process of isolation,Potato Dextrose Agar (PDA) medium was used to verify the fungal growth. Fungal strains were purified, and themorphological characterization of fungal strains was carried out at 10X and 100X by using a magnification microscope.The fungal strains, such as Aspergillus niger, Aspergillusoryzae, and Aspergillusflavus were identified. The stress offour types of dyes was given to each fungal strain. The results showed that Aspergillusoryzae was one of the moststable, non-toxic, and resistant fungal species against the high stress of dyes as compared to other species.

Production of inulin- and neolevan-type fructooligosaccharides by Penicillium janczewskii Zaleski CCIBt 3352.

Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of β-fructosyltransferases and/or β-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in a medium supplemented with sucrose concentrations of 30, 100, or 150gL-1 . Hydrolytic activity on sucrose was observed in culture filtrates; however, at 150gL-1 sucrose, the accumulation of 8g L-1 1-kestose (inulin-type FOS) and 7.3gL-1 neokestose (neolevan-type FOS) was observed, the latter being a type of FOS not commonly produced by filamentous fungi. In addition, minor amounts of four unidentified oligosaccharides, with a high degree of polymerization, were detected. The production of FOS was also observed in enzymatic assays, indicating the presence of extracellular enzymes with transfructosylating activity in the culture filtrates. Our findings demonstrate the feasibility of isolating promising microorganisms, for the production of FOS-synthesizing enzymes, from the rhizosphere of fructan-producing plants of the Brazilian Cerrado.

The Influence of Trace Elements on Anaerobic Digestion Process

The article is the literature review on the importance of trace elements supplementation in the methane fermentation process. The production of biogas, including methane, as well as the efficiency of the process depend on the substrates to be fermented. Substances supplied with the substrate as well as products generated in the decomposition phases can inhibit the process. The factor limiting fermentation is the rate of enzymatic hydrolysis of substrates. Certain compounds, such as alkanes, alkenes, biphenol, aromatic hydrocarbons, alcohols and ketones, are not directly susceptible to hydrolysis. They undergo this process in the presence of extracellular enzymes. The instability of the methane fermentation process described in the literature may be related to the lack of trace elements or micronutrients. Trace elements (Co, Ni, Cu, Mn, Fe, Zn, Se and Mo) are components of enzymes, some bacterial nucleic acids and essential for the synthesis of vitamins. The role of some trace elements, eg. Fe or Mo, has been well understood, while the importance of others still needs to be clarified. Literature data indicate that supplementing trace elements not only prevents process inhibition, but can also improve its performance by providing higher methane production.

Genotyping and distribution of virulence factors in V. parahaemolyticus from seafood and environmental sources, South-west coast of India

Vibrio parahaemolyticus,found naturally in warm coastal and estuarine waters worldwide is a leading cause of seafood-borne gastroenteritis and mass mortality among marine fishes and invertebrates. The present study focused on genotyping and virulence characterization of V. parahaemolyticus isolated from food and environmental sources along the South west coast of India, for surveillance of possible public health risk. Molecular typing was performed using ERIC-PCR. The potential pathogenicity of the strains was evaluated by screening for presence of type III secretion systems (T3SSs) genes. The presence of extracellular enzymes was also investigated Results revealed high level of genetic heterogeneity among the V. parahaemolyticus strains. Urease and hemolysin production observed in few strains is to be highlighted, since these phenotypic features are typical for clinical isolates of V. parahaemolyticus. All the strains carried the T3SS1 gene. T3SS2 β gene was present in majority of the strains whereas, T3SS2 α gene was not detected in any. Presence of genetically heterogeneous V. parahaemolyticus strains with virulence potential predicts high risk of disease outbreak associated with this pathogen in Cochin. The study emphasizes the need for a continuous surveillance of water systems in Cochin estuary and adjoining aquaculture farms.

Environmental DNA: ecological and genetic aspects

Attention to environmental DNA (eDNA) was motivated by problem of undesirable gene transfer possibility from genetically modified plants to wild bacteria and other organisms. First studies have already examined persistence of DNA from these plants in soil, and also in the samples of nearby groundwater and river for a few kilometers from the place of cultivating. In soil it persists long time enough – from a few days to a few years, and in water – from a few hours to a few days. eDNA excreted from different sources – frozen ice cores, sediments of lakes, soil, caves, water of lakes, rivers and oceans, contains genetic information about biodiversity of present and ancient organisms. Researches revealed an important fact: data of eDNA and other sources, for example pollen, macrofossils, living animals and plants, complement each other, showing more reliable information about the variety of species, than used separately. Therefore the analysis eDNA needs to be not of considered alternative method of ecological researches, but an additional to traditional methods. In the process of study of eDNA it is necessary to take into account five aspects at least: its origin, physical state, conversion, transport and technical challenges. The origin of eDNA remains studied not enough. From a few publications it is known that eDNA comes in different composition excretions, leaves, hair, peeling etc., or as a result of released plasmids and chromosomal DNA from living prokaryotes. There are also possible secondary sources of eDNA – dead bodies and excretions of predators, scavengers, detritivores and coprovores. On the amount of the genetic material, released by organisms in an environment, various ontogenetic, trophic and other factors can have considerably influence. eDNA can be presented in both intracellular and extracellular forms.. Over time intracellular eDNA releases outside by influence of different ecological factors – activity of microorganisms, presence of extracellular enzymes, mechanical destruction etc. In further extracellular eDNA can break in corpuscles of different sizes – mainly within the limits of 1–10 μm. It can be free, adsorbed by other substances or dissolved. At certain conditions the period of eDNA persistence can be very great – from a few hours (in water) to hundred thousands of years (in frozen ice cores). Ancient eDNA is very fragmented and chemically changed by various physical, chemical and biological factors of environment. Substantive eDNA amount is taken up by bacteria and protozoa. Here it quickly metabolizes, but some its fragments can be integrated in a local genome. eDNA is able to be transported to great distance (from a few meters to 10 kilometers) that can appreciably influence on the results of its research. Also the laboratory experiment has certain problems – design (equipment, sequence of operations and condition of it realization), realization of experiment, authenticity of it will depend on quality of equipment and reagents, competence and honesty of scientific personnel etc.), ability of skilled researcher to give interpretation of results. Data that given in our review testifies that the active study of eDNA only began, and further intensive efforts of environmentalists and geneticists are needed in direction of it research. The results of such researches will allow to create the effective methods of scientifically reasonable recreating nature application.

Biodegradation and hydrolysis rate of aliphatic aromatic polyester

The biodegradation and hydrolysis rates of an aliphatic aromatic copolyester were measured in manure, food, and yard compost environments and in phosphate buffer solution (pH = 8.0) and vermiculite at 58 °C. Mineralization, molecular weight reduction, and structural changes determined by DSC, FTIR, and 1H NMR were used as indicators of the biodegradation and hydrolysis rates. Poly(butylene adipate- co-terephthalate), PBAT, film biodegraded at distinctive rates in manure, food, and yard compost environments having different microbial activities. The highest biodegradation rate was found in manure compost, which had the highest CO 2 emissions and lowest C/N ratio. The possible presence of extracellular enzymes in manure and food composts may facilitate the hydrolytic reaction since greater molecular weight reduction rates were observed in these composts. 1H NMR and thermal analysis revealed that, while PBAT is a semi-crystalline copolyester with cocrystallization of BT and BA dimers, the soft aliphatic domain (BA) and the amorphous region are more susceptible to hydrolysis and biodegradation than the rigid aromatic domain (BT) and the crystalline region.

Design and development of hydrogel beads for targeted drug delivery to the colon.

The purpose of this research was to develop and evaluate a multiparticulate system of chitosan hydrogel beads exploiting pH-sensitive property and specific biodegradability for colon-targeted delivery of satranidazole. Chitosan hydrogel beads were prepared by the cross-linking method followed by enteric coating with Eudragit S100. All formulations were evaluated for particle size, encapsulation efficiency, swellability, and in vitro drug release. The size of the beads was found to range from 1.04 +/- 0.82 mm to 1.95 +/- 0.05 mm. The amount of the drug released after 24 hours from the formulation was found to be 97.67% +/- 1.25% in the presence of extracellular enzymes as compared with 64.71% +/- 1.91% and 96.52% +/- 1.81% release of drug after 3 and 6 days of enzyme induction, respectively, in the presence of 4% cecal content. Degradation of the chitosan hydrogel beads in the presence of extracellular enzymes as compared with rat cecal and colonic enzymes indicates the potential of this multiparticulate system to serve as a carrier to deliver macromolecules specifically to the colon and can be offered as a substitute in vitro system for performing degradation studies. Studies demonstrated that orally administered chitosan hydrogel beads can be used effectively for the delivery of drug to the colon.

Co-digestion of primary sewage sludge and industrial wastewater under anaerobic sulphate reducing conditions: enzymatic profiles in a recycling sludge bed reactor

The first stage in the degradation and recycling of primary sewage sludge and particulate organic matter is the solubilisation and enhanced hydrolysis of complex polymeric organic carbon structures associated with the anaerobic sulphidogenic environment. Solubilization of complex carbon substrates provides the primary reaction in the BioSURE Process, and is effected in the recycling sludge bed reactor (RSBR). During the process of anaerobic digestion, macromolecules are broken down into simpler low molecular weight compounds in the presence of extracellular enzymes. Though hydrolysis of the complex carbon sources was enhanced under biosulphidogenic conditions, no studies have examined the role of enzymes and the enzymatic profiles in the RSBR. To investigate the overall enzymology in the RSBR variations in COD, pH, sulphate, sulphite and sulphide concentrations, carbohydrates, protein and activities of glucosidases, proteases and lipases were studied over 50 days in the reactor at three different depths: 0-16 cm; 16-32 cm; 32-50 cm. While the pH profile remained fairly constant between 6.9 and 7.3 the sulphate and sulphide levels, as expected, changed dramatically as sulphate reduction took place. Proteases, lipases and glucosidases all showed enhanced activity with depth in the bioreactor. It is suggested that the increased sulphide concentration generated during the sulphate reduction process stimulates the enzymes, leading to enhanced solubilisation of primary sewage sludge.

Characterization of soil samples by enzyme activity

The presence of extracellular enzymes in soils offers a very sensitive method for matching, or distinguishing between, soil samples. Simple colorimetric methods were used to compare enzyme levels in soils from different sites. It was found that each soil tested had its own spectrum of enzyme activity, by which it could be characterized and thereby distinguished from other soils that were essentially similar in physical composition. This article describes nine simple enzyme assays used by sixth-form students for ‘soilprinting’, and draws attention to some possible applications of the technique in forensic science and in studies of soil fertility.

Digestion of polysaccharide constituents of tropical pasture herbage in the bovine rumen : Part IV. The hydrolysis of hemicelluloses from spear grass by cell-free enzyme systems from rumen fluid

Hemicelluloses have been isolated from spear grass ( Heteropogon contortus), before and after digestion in the rumen, and separated into linear and branched fractions. Similar fractions have also been obtained from a pasture sample and from the faeces fibre of a steer fed on the same pasture. The rates of hydrolysis of all of these hemicellulose fractions have been determined in the presence of extracellular enzymes from rumen fluid and of enzymes liberated by disruption of rumen microorganisms. The oligosaccharide products of such enzymic degradations have been partially identified. The results confirm that the incomplete digestion of hemicelluloses in the rumen is due to physical protection ( e.g. by lignin), rather than to structural differences between different components of the hemicelluloses. There is no difference between rates of digestion of branched and linear hemicelluloses, and previous results which indicated such differences were probably caused by presence of a readily digested glucan in linear hemicellulose fractions.