Presence Of Antibiotics In Environment Research Articles

Urbanization and land use regulate soil vulnerability to antibiotic contamination in urban green spaces

The presence of antibiotics in environment is an emerging concern because of their ubiquitous occurrence, adverse eco-toxicological effects, and promotion of widespread antibiotic resistance. Urban soil, which plays a noticeable role in human health, may be a reservoir of antibiotics because of intensive human disturbance. However, little is understood about the vulnerability of soil to antibiotic contamination in urban areas and the spatial–temporal characteristics of anthropogenic and environmental pressures. In this study, we developed a framework for the dynamic assessment of soil vulnerability to antibiotic contamination in urban green spaces, combining antibiotic release, exposure, and consequence layers. According to the results, soil vulnerability risks shown obvious spatial–temporal variation in urban areas. Areas at a high risk of antibiotic contamination were usually found in urban centers with high population densities and in seasons with low temperature and vegetation coverage. Quinolones (e.g., ofloxacin and norfloxacin) were priority antibiotics that posed the highest vulnerability risks, followed by tetracyclines. We also confirmed the effectiveness of the vulnerability assessment by correlating soil vulnerability indexes and antibiotic residues in urban soils. Furthermore, urbanization- and land use-related parameters were shown to be critical in regulating soil vulnerability to antibiotic contamination based on sensitivity analysis. These findings have important implications for the prediction and mitigation of urban soil contamination with antibiotics and strategies to improve human health.

Study the Expression of ompf Gene in Esherichia coli Mutants.

The outer membrane porin proteins are the major factors in controlling the permeability of cell membrane. OmpF is an example of porin proteins in Esherichia coli. In normal growth condition a large amount of this protein is synthesised, but under stress condition, such as the presence of antibiotics in environment its expression is decreased inhibiting the entrance of antibiotics into cell. The expression of ompF is inhibited by antisense RNA transcribed from micF. In normal condition the expression of micF is low, but in the presence of antibiotics its expression is increased and causes multiple resistances to irrelevant antibiotics. The aims of this research were to study first, the intactness of micF and then quantify the expression of ompF in ciprofloxacin and tetracycline resistant mutants of E. coli. For this purpose the 5' end of micF was amplified and then sequenced. None of these mutants except one and its clone has a mutation in this gene. Then the relative expression of ompF in these mutants was quantified by real time PCR. There was no significant difference between ompF transcription of mutants and wild type strain. Based on this study and previous study it is concluded that low to intermediate levels of resistance to ciprofloxacin and tetracycline does not decrease ompF transcription.

Corneal Inflammatory Diseases - Infectious Keratitis in Dogs

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