Introduction: Students who are pre-health experience higher levels of stress and adopt maladaptive coping behaviors including alcohol and drug use. Additionally, students belonging to sexual and gender minority (SGM) groups are more likely to drop out from science, technology, engineering, and math (STEM) majors. Studies have yet to determine how SGM identities influence self-perceived ability and desire to pursue careers in healthcare. Hypothesis: Pre-health students belonging to SGM groups have lower self confidence in their career trajectory as a function of identity compared to their cisgender, heterosexual peers. Methods: Pre-health students at Boston University were surveyed using quantitative Likert-like items and open response questions. Survey content explored the degree to which students agreed with statements regarding how their sexual orientation and gender has affected their career trajectory as a pre-health student. Responses were grouped by gender and sexual orientation and unpaired Mann-Whitney rank tests were used to assess differences at an alpha of 0.05. Results: Of the 71 respondents, 44 (61.97%) identified as heterosexual women, 14 (19.71%) identified as queer women, 10 (14.08%) identified as heterosexual men and 3 (4.22%) identified as queer men. Queer women, compared to heterosexual women, reported higher perceived effects of sexual orientation (p=0.0228, median 2.0, IQR 1-2 vs. 1.0, IQR 1-2) and gender identity (p=0.0079, median 2.0, IQR 1-3.5, vs. median 1.0, IQR 1-2) on career trajectory. There were no significant differences between responses from queer men and heterosexual men regarding perceived effect of sexual orientation and gender identity on their career trajectories. Conclusion: Queer women in pre-health tracks may benefit most from targeted supportive intervention. This study was unfunded. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.