Cleavage Plane Orientation Research Articles

The development of laser cutting and drilling processes for thick Lithium Niobate wafers for electronic sensor applications

Lithium Niobate (LiNbO3) is a CVD grown synthetic salt having a trigonal crystal structure consisting of niobium, lithium, and oxygen. Due to its variety of mechanical and optical properties, single crystal LiNbO3 finds end use applications in optical waveguides, piezoelectric sensors, optical modulators, and electronic oscillators.LiNbO3 wafers are traditionally mechanically processed using abrasive disks and wire saws, and as a result of the inherently low fracture toughness cut-edge chipping and fracture occur lowering process yields. This study aimed to develop a laser process for the cutting, profiling and drilling of thick whole LiNbO3 wafers, using a femtosecond pulsed 515 nm wavelength source. The stresses induced during processing were controlled through a combination of wafer annealing, crystallographic (012) and (010) cleavage plane orientation. Process generated thermal gradients were controlled using beam manipulation and a water spray liquid flow process. The developed processes substantially reduced material fracture and significantly enhanced cut-edge quality, elevating yields above 80%.

On rate dependence and anisotropy in phase field modeling of polycrystalline fracture

A phase field theory of fracture incorporating rate dependence (i.e., kinetic viscosity) and anisotropic elasticity is implemented to study strength and failure modes in polycrystalline materials. After presentation of the constitutive framework, a new analytical solution is derived for one-dimensional (1D) homogeneous loading at constant strain rate, wherein crack viscosity is enabled. The three-dimensional (3D) theory is implemented in a finite element (FE) context, with static and dynamic solutions verified up to peak loading for homogeneous microstructures. Extensive simulations of heterogeneous polycrystalline microstructures follow, where baseline material properties correspond to either boron carbide (B4C) or titanium diboride (TiB2). Results quantify effects of loading rate, elastic anisotropy, cleavage energy anisotropy, and grain boundary surface energy on tensile strength. Notable are effects of strong basal texture, which influences average strength via both elastic anisotropy and basal cleavage plane orientation. Effects of loading rate are naturally more prominent for materials that fail mostly intergranularly rather than transgranularly, in agreement with experimental observations.

The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus.

SummaryRadial glia-like (RGL) stem cells persist in the adult mammalian hippocampus, where they generate new neurons and astrocytes throughout life. The process of adult neurogenesis is well documented, but cell-autonomous factors regulating neuronal and astroglial differentiation are incompletely understood. Here, we evaluate the functions of the transcription factor zinc-finger E-box binding homeobox 1 (ZEB1) in adult hippocampal RGL cells using a conditional-inducible mouse model. We find that ZEB1 is necessary for self-renewal of active RGL cells. Genetic deletion of Zeb1 causes a shift toward symmetric cell division that consumes the RGL cell and generates pro-neuronal progenies, resulting in an increase of newborn neurons and a decrease of newly generated astrocytes. We identify ZEB1 as positive regulator of the ets-domain transcription factor ETV5 that is critical for asymmetric division.

Altered cleavage plane orientation with increased genomic aneuploidy produced by receptor-mediated lysophosphatidic acid (LPA) signaling in mouse cerebral cortical neural progenitor cells

The brain is composed of cells having distinct genomic DNA sequences that arise post-zygotically, known as somatic genomic mosaicism (SGM). One form of SGM is aneuploidy—the gain and/or loss of chromosomes—which is associated with mitotic spindle defects. The mitotic spindle orientation determines cleavage plane positioning and, therefore, neural progenitor cell (NPC) fate during cerebral cortical development. Here we report receptor-mediated signaling by lysophosphatidic acid (LPA) as a novel extracellular signal that influences cleavage plane orientation and produces alterations in SGM by inducing aneuploidy during murine cortical neurogenesis. LPA is a bioactive lipid whose actions are mediated by six G protein-coupled receptors, LPA1–LPA6. RNAscope and qPCR assessment of all six LPA receptor genes, and exogenous LPA exposure in LPA receptor (Lpar)-null mice, revealed involvement of Lpar1 and Lpar2 in the orientation of the mitotic spindle. Lpar1 signaling increased non-vertical cleavage in vivo by disrupting cell–cell adhesion, leading to breakdown of the ependymal cell layer. In addition, genomic alterations were significantly increased after LPA exposure, through production of chromosomal aneuploidy in NPCs. These results identify LPA as a receptor-mediated signal that alters both NPC fate and genomes during cortical neurogenesis, thus representing an extracellular signaling mechanism that can produce stable genomic changes in NPCs and their progeny. Normal LPA signaling in early life could therefore influence both the developing and adult brain, whereas its pathological disruption could contribute to a range of neurological and psychiatric diseases, via long-lasting somatic genomic alterations.

Spindle Orientation-Independent Control of Cell Fate Determination by RGS3 and KIF20A.

It was proposed that similar to its role in the invertebrate nervous system, mitotic spindle orientation (or cell cleavage plane orientation) of a dividing neural progenitor cell specifies the fate of daughter cells in the mammalian brain, modulating the production of neurons via symmetric versus asymmetric cell divisions during the course of neurogenesis. Experimental tests of the sufficiency of spindle/cleavage plane orientation in mammalian cell fate determination have yielded conflicting results. On the other hand, the necessity of spindle/cleavage plane orientation in mammalian cell fate determination has not yet been addressed. Here we examined the necessity of spindle/cleavage plane orientation during cortical neurogenesis in mice with loss-of-function of the RGS3-KIF20A interaction axis. We present evidence that while inactivation of RGS3 or KIF20A was linked to a shift in neural progenitor cells from proliferative to differentiative divisions in the developing cortex, these genetic mutations did not lead to anticipated alteration in the orientation of spindle/cleavage plane. Our results indicate that the RGS3-KIF20A axis regulates the balance between proliferation and differentiation in the mammalian cortex employing a mechanism independent of spindle/cleavage plane orientation. These data also caution against using spindle/cleavage plane orientation as the synonym for cell fate determination.

Structure formation by hot extrusion of thermoelectric bismuth chalcogenide solid solution rods

Major advantage of extruded Bi2Te3 based thermoelectric materials is high mechanical strength compared with that of melt-crystallized materials. Mechanical properties are of special importance for thermogenerator module applications where thermogenerator branches may undergo elevated thermal stresses due to large temperature differences at the modules. Since extrusion is typically a high-temperature process the structure of extruded materials is controlled by the plastic deformation in multiple slip systems resulting in the formation of a final deformed structure. The grain orientations are predominantly such that the most probable cleavage plane orientation is parallel to the extrusion axis. Recovery processes occur simultaneously and different recrystallization stages may take place. In the latter case the deformed texture may be destroyed. Structure evolution along the extruded rod of Bi2Se0.3Te2.7 ternary solid solution was studied with metallography and X-ray diffraction. Extrusion was interrupted for the study and so the specimen was a whole rod the initial part of which was the extrusion billet and the final part was the as-extruded material. The structure of the material is formed by competitive processes of dislocation generation and annealing. The plastic deformation energy is the highest in the extruder zone of the rod. Both the hardening processes and the texture are controlled by the plastic deformation mechanism. Plastic deformation is accompanied by generation of defects that are most likely vacancy type ones.

Blastomere cleavage plane orientation and the tetrahedral formation are associated with increased probability of a good-quality blastocyst for cryopreservation or transfer: a time-lapse study

To determine whether blastomere spatial arrangement in early human embryos is reflective of embryonic potential. Retrospective analysis of prospectively collected data. Single academic center. Patients undergoing a single blastocyst transfer. None. Developmental kinetics, blastocyst quality, embryo dysmorphisms, and live birth rate. A total of 716 embryos were examined in detail for cleavage plane orientation, blastomere arrangement, and morphokinetic behavior. Tetrahedral (TET) and nontetrahedral embryos (nTET) differed significantly in developmental kinetics. The frequency of dysmorphisms, multinucleation, and irregular chaotic division was higher in nTET embryos. Only 44% of nTET versus 62.9% of TET embryos were scored as top-quality blastocysts. After adjusting for age, our data indicated that having TET embryos significantly increased the odds of having a blastocyst for cryopreservation/transfer (odds ratio, 3.58; confidence interval, 2.42-5.28) when compared with nTET. A total of 164 fresh single ETs were performed with blastocyst-stage embryos. The implantation rate for TET- and nTET-derived blastocysts were similar (64.7% and 62%, respectively). The live birth rate was 55% in both groups. A meridonal first division was noted in 85% of the fresh SET blastocysts. Cleavage plane orientation during the first three divisions appeared to dictate final blastomere spatial arrangement. The TET formation at the four-cell stage was predictive for embryos most likely to develop into good-quality blastocysts for cryopreservation/transfer. Morphokinetic markers of embryo potential were significantly different between TET and nTET embryos.

The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART.

Assisted reproduction technology offers the opportunity to observe the very early stages of human development. However, due to practical constraints, for decades morphological examination of embryo development has been undertaken at a few isolated time points at the stages of fertilisation (Day 1), cleavage (Day 2-3) and blastocyst (Day 5-6). Rather surprisingly, the morula stage (Day 3-4) has been so far neglected, despite its involvement in crucial cellular processes and developmental decisions. The objective of this review is to collate novel and unsuspected insights into developmental processes occurring during formation of the morula, highlighting the key importance of this stage for a better understanding of preimplantation development and an improvement of ART. PubMed was used to search the MEDLINE database for peer-reviewed English-language original articles and reviews concerning the morula stage in mammals. Searches were performed by adopting 'embryo', 'morula', 'compaction', 'cell fate' and 'IVF/assisted reproduction' as main terms, in association with other keywords expressing concepts relevant to the subject (e.g. cell polarity). The most relevant publications, i.e. those concerning major phenomena occurring during formation of the morula in established experimental models and the human species, were assessed and discussed critically. Novel live cell imaging technologies and cell biology studies have extended our understanding of morula formation as a key stage for the development of the blastocyst and determination of the inner cell mass (ICM) and the trophectoderm (TE). Cellular processes, such as dynamic formation of filopodia and cytoskeleton-mediated zippering cell-to-cell interactions, intervene to allow cell compaction (a geometrical requisite essential for development) and formation of the blastocoel, respectively. At the same time, differential orientation of cleavage planes, cell polarity and cortical tensile forces interact and cooperate to position blastomeres either internally or externally, thereby influencing their cellular fate. Recent time lapse microscopy (TLM) observations also suggest that in the human the process of compaction may represent an important checkpoint for embryo viability, through which chromosomally abnormal blastomeres are sensed and eliminated by the embryo. In clinical embryology, the morula stage has been always perceived as a 'black box' in the continuum of preimplantation development. This has dictated its virtual exclusion from mainstream ART procedures. Recent findings described in this review indicate that the morula, and the associated process of compaction, as a crucial stage not only for the formation of the blastocyst, but also for the health of the conceptus. This understanding may open new avenues for innovative approaches to embryo manipulation, assessment and treatment.

Zeb1 is important for proper cleavage plane orientation of dividing progenitors and neuronal migration in the mouse neocortex.

During neocortical development, there are two important events, including expansion of the neural progenitor pool through symmetric divisions, and generation of neurons via asymmetrical divisions that lead to a serial process of neuronal polarization, migration, and layer-type specific phenotype acquisition. The mechanisms underlying these processes remain poorly elucidated. Here, we show that the transcription factor Zeb1 regulates the orientation of the cleavage plane of dividing neural progenitors, neuronal polarity, and migration. Upon Zeb1 removal, the cleavage plane of mitotic neural progenitors fails to orientate vertically, resulting in random orientation and premature neuronal differentiation. Consequently, these extra number of precociously produced neurons migrate aberrantly to the upper layer. Mechanistically, we show that Zeb1 suppresses Pak3, a p21-activated serine/threonine protein kinase, through formation of a functional repressing complex together with methyltransferase PRMT5 and Pak3. Our results reveal that Zeb1 plays an essential role in neocortical development and may provide insights into the mechanisms responsible for cortical developmental diseases.

Mechanical characterizations of single-crystalline (Cu, Ni)6Sn5 through uniaxial micro-compression

Microelectronic industry is experiencing a major paradigm shift from the conventional two-dimensional into hierarchical three-dimensional integrated circuits (3D ICs). In 3D IC technology, through-silicon-vias and micro joints are regarded as the two vital interconnection processes to achieve higher packing density as well as heterogeneous integration. However, the miniaturization of solder joints down to micron level causes the consequence that intermetallic compounds takes up most of the volume of micro joints within a short period of time, in stark contrast to the well-known scenario at the opposite end of spectrum that soft Sn-based solders always occupies a high volume fraction of joints. Thus, intermetallics are expected to shoulder primary responsibilities of mechanical reliability in micro joints. Therefore, a full understanding about the mechanical behaviors of intermetallics at micron scale is essential for validity and reliability evaluation of 3D IC interconnections. The present study focuses on mechanical assessment of single-crystalline (Cu, Ni)6Sn5 by means of uniaxial micro-compression and nanoindentation. The anisotropic mechanical properties of hexagonal Cu6Sn5 are investigated by compression testing on single-crystalline Cu6Sn5 micropillars with different grain orientations. The jammed network of dislocation inside deformed micropillars suggests that intermittent strain bursts in stress-strain curves are relevant to dislocation-avalanche mechanism. In addition, Cu6Sn5 micropillars are found to exhibit brittle cleavage failure with most fractured pieces crushed in the same direction, and one set of preferred cleavage plane are characterized by post-mortem analysis. Moreover, the strengthening and phase-stabilizing effect of Ni element on Cu6Sn5 is also investigated by micropillar compression and X-ray diffraction, respectively.

Analytical prediction for depth of subsurface damage in silicon wafer due to self-rotating grinding process

Subsurface damage (SSD) induced by silicon wafer grinding process is an unavoidable problem in semiconductor manufacturing. Although experimental attempts have been made on investigation of the influential factors on the SSD depth, however, few theoretical studies have been conducted to obtain SSD depth through grinding parameters. To fill the gap, an analytical model is developed to predict the SSD depth in silicon wafer due to self-rotating grinding process, which can reveal the relationship among SSD depth and the grinding parameters, the size of the abrasive grains and the radial distance from the wafer center. The establishment of the proposed model is based on scratch theory and fracture mechanics of isotropic brittle materials, and we further consider the effects of elastic recovery, cleavage plane and crystalline orientation on SSD formation. To validate the applicability of the proposed predictive model, grinding experiments with varied grinding parameters are performed and the depths of SSD along the <110> and <100> crystal directions are also measured and analyzed. The results given by the proposed model present reasonable accuracy of less than 20% deviation with experimental results. Effects of grinding parameters, wafer radial distance, crystalline orientation, and abrasive grain size on SSD depth are discussed in detail.

Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains.

The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells within the Outer Proliferation Center (OPC) of Drosophila larval brains.

Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding

SummaryThe developmental mechanisms regulating the number of adult neural stem cells (aNSCs) are largely unknown. Here we show that the cleavage plane orientation in murine embryonic radial glia cells (RGCs) regulates the number of aNSCs in the lateral ganglionic eminence (LGE). Randomizing spindle orientation in RGCs by overexpression of Insc or a dominant-negative form of Lgn (dnLgn) reduces the frequency of self-renewing asymmetric divisions while favoring symmetric divisions generating two SNPs. Importantly, these changes during embryonic development result in reduced seeding of aNSCs. Interestingly, no effects on aNSC numbers were observed when Insc was overexpressed in postnatal RGCs or aNSCs. These data suggest a new mechanism for controlling aNSC numbers and show that the role of spindle orientation during brain development is highly time and region dependent.

A Butterfly Effect on Neural Stem Cells

Adult neural stem cells originate from the embryonic brain, but the underlying mechanisms remain poorly known. In this issue of Neuron, Falk etal. (2017) reveal how the timely control of cleavage plane orientation during division of embryonic neural progenitors has a specific and long-lasting impact on adult neurogenesis.

APLIKACE TEXTURNÍ RTG-DIFRAKČNÍ ANALÝZY V TEKTONICE – KVANTIFIKACE PŘEDNOSTNÍ ORIENTACE KALCITU V KARBONÁTOVÝCH HORNINÁCH

The goal of this work was to apply texture x-ray diffraction analysis to study naturally strained rocks, in which the quantification of main preferred orientation cannot be conducted by the optical methods. This method has mainly been developed for metallography and its application in geology has been very limited so far. Samples of the fine-grained limestone have been collected from an outcrop, in which the direction of tectonic movement has been known. Thus, the tectonic situation could be correlated with the data obtained by XRD texture analysis. Analyses have been done by two devices with different geometry of experiment. The first experiment (Schulz reflection geometry) needed correction for the gain data, because of tilting of the sample, which led to the misalignment of the sample from the x-ray beam direction. The second one (in-plane geometry) has been measured, when the sample has been fixed and rotated, thus the correction was not needed. The results in a form of pole figures reflect the mechanism of deformation. The orientation of cleavage planes of calcite parallel to foliation indicates a cataclastic flow. Thus, the method could be used to study deformation mechanisms. The asymmetry of the results can show sense of shear, but it could also reflect inhomogenities of the samples.

Cleavage of Human Embryos: Options and Diversity

In order to estimate the diversity of embryo cleavage relatives to embryo progress (blastocyst formation), time-lapse imaging data of preimplantation human embryo development were used. This retrospective study is focused on the topographic features and time parameters of the cleavages, with particular emphasis on the lengths of cleavage cycles and the genealogy of blastomeres in 2- to 8-cell human embryos. We have found that all 4-cell human embryos have four developmental variants that are based on the sequence of appearance and orientation of cleavage planes during embryo cleavage from 2 to 4 blastomeres. Each variant of cleavage shows a strong correlation with further developmental dynamics of the embryos (different cleavage cycle characteristics as well as lengths of blastomere cycles). An analysis of the sequence of human blastomere divisions allowed us to postulate that the effects of zygotic determinants are eliminated as a result of cleavage, and that, thereafter, blastomeres acquire the ability of own syntheses, regulation, polarization, formation of functional contacts, and, finally, of specific differentiation. This data on the early development of human embryos obtained using noninvasive methods complements and extend our understanding of the embryogenesis of eutherian mammals and may be applied in the practice of reproductive technologies.

Mapping neurogenesis onset in the optic tectum of Xenopus laevis.

Neural progenitor cells have a central role in the development and evolution of the vertebrate brain. During early brain development, neural progenitors first expand their numbers through repeated proliferative divisions and then begin to exhibit neurogenic divisions. The transparent and experimentally accessible optic tectum of Xenopus laevis is an excellent model system for the study of the cell biology of neurogenesis, but the precise spatial and temporal relationship between proliferative and neurogenic progenitors has not been explored in this system. Here we construct a spatial map of proliferative and neurogenic divisions through lineage tracing of individual progenitors and their progeny. We find a clear spatial separation of proliferative and neurogenic progenitors along the anterior-posterior axis of the optic tectum, with proliferative progenitors located more posteriorly and neurogenic progenitors located more anteriorly. Since individual progenitors are repositioned toward more anterior locations as they mature, this spatial separation likely reflects an increasing restriction in the proliferative potential of individual progenitors. We then examined whether the transition from proliferative to neurogenic behavior correlates with cellular properties that have previously been implicated in regulating neurogenesis onset. Our data reveal that the transition from proliferation to neurogenesis is associated with a small change in cleavage plane orientation and a more pronounced change in cell cycle kinetics in a manner reminiscent of observations from mammalian systems. Our findings highlight the potential to use the optic tectum of Xenopus laevis as an accessible system for the study of the cell biology of neurogenesis. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 76: 1328-1341, 2016.

Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex.

The evolutionary expansion of the neocortex in mammals has been linked to enlargement of the subventricular zone (SVZ) and increased proliferative capacity of basal progenitors (BPs), notably basal radial glia (bRG). The transcription factor Pax6 is known to be highly expressed in primate, but not mouse, BPs. Here, we demonstrate that sustaining Pax6 expression selectively in BP-genic apical radial glia (aRG) and their BP progeny of embryonic mouse neocortex suffices to induce primate-like progenitor behaviour. Specifically, we conditionally expressed Pax6 by in utero electroporation using a novel, Tis21–CreERT2 mouse line. This expression altered aRG cleavage plane orientation to promote bRG generation, increased cell-cycle re-entry of BPs, and ultimately increased upper-layer neuron production. Upper-layer neuron production was also increased in double-transgenic mouse embryos with sustained Pax6 expression in the neurogenic lineage. Strikingly, increased BPs existed not only in the SVZ but also in the intermediate zone of the neocortex of these double-transgenic mouse embryos. In mutant mouse embryos lacking functional Pax6, the proportion of bRG among BPs was reduced. Our data identify specific Pax6 effects in BPs and imply that sustaining this Pax6 function in BPs could be a key aspect of SVZ enlargement and, consequently, the evolutionary expansion of the neocortex.

Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1.

The proneural factor Ascl1 controls multiple steps of neurogenesis in the embryonic brain, including progenitor division and neuronal migration. Here we show that Cenpj, also known as CPAP, a microcephaly gene, is a transcriptional target of Ascl1 in the embryonic cerebral cortex. We have characterized the role of Cenpj during cortical development by in utero electroporation knockdown and found that silencing Cenpj in the ventricular zone disrupts centrosome biogenesis and randomizes the cleavage plane orientation of radial glia progenitors. Moreover, we show that downregulation of Cenpj in post-mitotic neurons increases stable microtubules and leads to slower neuronal migration, abnormal centrosome position and aberrant neuronal morphology. Moreover, rescue experiments shows that Cenpj mediates the role of Ascl1 in centrosome biogenesis in progenitor cells and in microtubule dynamics in migrating neurons. These data provide insights into genetic pathways controlling cortical development and primary microcephaly observed in humans with mutations in Cenpj.

Mica Inclusions inside Host Mica Grains from the Sutlej Section of the Higher Himalayan Crystallines, India-Morphology and Constrains in Genesis

Biotite and muscovite inclusions inside mica host minerals from the Sutlej section of the Higher Himalayan Crystalline were studied under an optical microscope. These inclusions formed possibly by local recrystallization of mica grains during regional prograde metamorphism, with some affected by top-to-SW shear leading to parallelogram shapes. Recrystallization may have been assisted by solution transfer along the cleavage planes of the host grains. The relative competency of deformed phyllosilicate inclusions with the same or different composition to the host depends on the size and orientation of (001) cleavage planes of the inclusions relative to the host. Shearing of mica inclusions led to their parallelogram geometries within the contained mica inclusions. Some of the sheared inclusions deflect cleavage planes in the host minerals and define flanking microstructures. Trapezoid-shaped inclusions are a new finding that deserves more attention for their genesis. These structurally anisotropic inclusions did not originate from sub-grains, secondary infillings or retrogression. These inclusions are also not related to pseudomorphism, isomorphism, folding of the bulk rock etc. Some of the inclusions formed by recrystallization of the host mineral during top-to-SW ductile shear.