Predominant Polyunsaturated Fatty Acid Research Articles

Replacement of saturated fatty acids with linoleic acid in western diet attenuates atherosclerosis in a mouse model with inducible ablation of hepatic LDL receptor

Dietary saturate fatty acids (SFAs) have been consistently linked to atherosclerosis and obesity, both of which are characterized by chronic inflammation and impaired lipid metabolism. In comparison, the effects of linoleic acid (LA), the predominant polyunsaturated fatty acid in the Western diet, seem to diverge. Data from human studies suggest a positive association between high dietary intake of LA and the improvement of cardiovascular risk. However, excessive LA intake has been implicated in the development of obesity. Concerns have also been raised on the potential pro-inflammatory properties of LA metabolites. Herein, by utilizing a mouse model with liver-specific Ldlr knockdown, we directly determined the effects of replacing SFAs with LA in a Western diet on the development of obesity and atherosclerosis. Specifically, mice treated with a Ldlr ASO were placed on a Western diet containing either SFA-rich butter (WD-B) or LA-rich corn oil (WD-CO) for 12 weeks. Despite of showing no changes in body weight gain or adiposity, mice on WD-CO exhibited significantly less atherosclerotic lesions compared to those on WD-B diet. Reduced lesion formation in the WD-CO-fed mice corresponded with a reduction of plasma triglyceride and cholesterol content, especially in VLDL and LDL, and ApoB protein levels. Although it increased expression of proinflammatory cytokines TNF-α and IL-6 in the liver, WD-CO did not appear to affect hepatic injury or damage when compared to WD-B. Collectively, our results indicate that replacing SFAs with LA in a Western diet could reduce the development of atherosclerosis independently of obesity.

Fatty acid profile and lipid oxidation of dry aged beef

The objective of this research was to determine the effect of the extended dry aging period (0, 20, 40, and 60 days) on lipid oxidation, fatty acid profile, and tenderness in selected dry aged Hanwoo cow beef cut loins. Dry aging regimes were 40C and 85% relative humidity. Lipid oxidation was determined by using 2-thiobarbituric acid reactive substances. Shear force values of the highly marbled beef decreased first 20d aging, and did not significantly change after that, until 60d. Hardness of texture profile analysis gradually decreased, but chewiness value did not differ. A total of 16 fatty acids were identified in dry-aged beef. The most predominant FAs in the aged beef were C18:1, C16:0, and C18:0. The C18:2 was the predominant polyunsaturated fatty acid in the samples. Total PUFA gradually and C18:2n-6 decreased (p<0.05) during aging. C20:1, C18:3n-3, C20:4 and C22:1 acid significantly decreased (p<0.05) until 20d aging and no changes were observed after 20d to 60d. 2-thiobarbituric acid reactive values of beef increased (p<0.001) during dry-aging days, dry aging for 40d and 60d resulted in excessive oxidation of lipids. Dry-aged meat is so popular because of its unique taste. Improvements in tenderness associated with the 20d aging of a beef subprimals, however, some lipid oxidation may be associated with the development of specific dry-aged beef flavor.

Occurrence and patterns of nutritional traits and polycyclic aromatic hydrocarbons (PAHs) in sea cucumber (Holothuria polii) tissues: benefits and risk for human health

Abstract Objectives The paper evaluates the benefit and risk for human health associated with consumption of sea cucumber Holothuria polii (H. polii) from Italian coasts (Central Mediterranean Sea). Materials and Methods Body wall (BW), internal tunic (ITu), muscle bands (MBs), alimentary canal (AC), gonad (Gd), and respiratory tree (RT) of H. polii were analyzed for proximate composition. Moreover, amino acids (AAs), fatty acids (FAs) and polycyclic aromatic hydrocarbons (PAHs) were determined with high-performance liquid chromatography coupled with ultraviolet-visible spectroscopy (UPLC UV/Vis), gas chromatography-flame ionization detector (GC-FID) and gas chromatography mass spectrometry (GC-MS), respectively. Results Differences in the contents of total amino acids (TAAs) occurred based on tissue and sex, with AC and MB of female and Gd of male showing higher contents (range 47.8–60.2 g/kg we weight (ww)). Glycine and glutamic acid were the most abundant. Polyunsaturated fatty acid (PUFA) was the major class of FAs and arachidonic acid and eicosapentenoic acid (EPA) were the predominant PUFA. n-3 PUFA showed higher content in Gd, AC, and RT, indicating higher quality. A favorable n-3/n-6 in the range of 1.04–1.67 was observed. PAHs showed values ranging from 23 to 207 µg/kg ww with the highest levels in Gd and AC tissues and the lower in BW. Benzo[a]pyrene, the most toxic compound, was detected in all tissues, of both sexes, at levels of 1.5–18 µg/kg ww. Conclusion All tissues of H. polii, although with differences among them, are valuable food and can contribute for a healthy diet. Excess cancer risk (CR) values for Gd and AC tissues were above the considerable CR threshold of one in 10 000 established by the United States Environmental Protection Agency (USEPA) for high ingestion rate of this seafood.

Thraustochytrids from the Red Sea mangroves in Saudi Arabia and their abilities to produce docosahexaenoic acid

AbstractThis is the first study of thraustochytrids from the Red Sea coast in Saudi Arabia. One hundred and thirty-four isolates of thraustochytids were cultured from Al-Leith, Jeddah and Yanbu mangroves from this area, and were categorized into 38 morphotypes. Among the isolated thraustochytrids,Aurantiochytriumwas the most speciose genus, represented by 36 morphological types. Seventeen strains formed a distinct clade within the genusAurantiochytriumbased on phylogenetic analyses of 18S rDNA sequences. TheAurantiochytriumclade from the Middle East is characterized by the production of high levels of oleic and linoleic acids and may represent undescribed taxa. FourAurantiochytriumstrains were grown on large scale to study their ability to produce docosahexaenoic acid (DHA). These strains produced biomass ranging from 37.7 to 66 g L−1and the percentages of oil ranged from 40 to 57.2% of the dry weight. Twenty-one fatty acids were recorded from the four strains which included: eight saturated four monounsaturated and nine polyunsaturated. Dominant fatty acids included C16:0 palmitic acid (24.14–37.02% total fatty acid (TFA)), C18:1ω9 oleic acid (3.01–25.07% TFA), C18:2ω6 linoleic acid (1.85–20.85% TFA) and C22:6ω3 DHA (4.87–16.5% TFA). DHA was the predominant polyunsaturated fatty acid in three strains, while linoleic acid was the predominant PUFAs in one strain.

Nordic cyanobacterial and algal lipids: Triacylglycerol accumulation, chemotaxonomy and bioindustrial potential.

The ability to capture and convert sunlight, water and nutrients into useful compounds make photosynthetic microbes ideal candidates for the bio-industrial factories of the future. However, the suitability of isolates from temperate regions to grow under Nordic conditions is questionable. In this work, we explore the chemotaxonomy of Nordic strains of cyanobacteria and one green alga and evaluate their potential as raw materials for the production of lipid-based bio-industrial compounds. Thin-layer chromatography was used to identify the presence of triacylglycerol, which were detected in the majority of strains. Fatty acid methyl ester profiles were analysed to determine the suitability of strains for the production of biodiesel or the production of polyunsaturated fatty acids for the nutraceutical industry. The Nordic Synechococcus strains were unique in demonstrating fatty acid profiles comprised mostly C14:0, C16:0 and C16:1 and lacking polyunsaturated fatty acids. These properties translated to superior predicted biodiesel qualities, including cetane number, cold filter plugging point and oxidative stability compared to the other evaluated strains. Polyunsaturated fatty acids were detected at high levels (38-53%), with Calothrix sp. 336/3 being abundant in two essential fatty acids, linoleic and alpha-linolenic acid (21 and 17%, respectively). Gamma-linoleic acid was the predominant polyunsaturated fatty acid for the remaining strains (13-21%). In addition to assessing the potential of Nordic strains for bio-industrial production, this work also discusses issues such as taxonomy and predictive modelling, which can affect the identification of prospective high-performing strains.

DYNAMIC CHANGES IN THE COMPOSITION OF BIOLOGICALLY ACTIVE COMPOUNDS OF BUPLEURUM SCORZONERIFOLIUM WILLD. AERIAL PART IN DIFFERENT PHENOLOGICAL PHASES

The article is devoted to investigate the changes in the composition of biologically active substances (essential oils, lipid fraction, phenolic compounds) of the aerial part of the Bupleurum scorzonerifolium Willd. in depending on the phase of plant development. The greatest variety of compounds included in the composition of essential oils is characterized by a flowering phase. Fifteen terpene compounds – p-cymol, β-myrcene, trans-β-ocymene, limonene, (E,E)-α-farnesene, α-copaene, β-elemene, humulene, germacrene D, caryophyllene, γ-muurolene, bicyclogermacrene, Δ-cadinene, t-muurolol, spatulenol – are constant components of essential oils, regardless of phenophase. The quantitative content of these components varies from trace to major at different phenological phases. The coincidence of the main components of the lipid fraction was established. The main saturated acid is 16:0. Its content changes depending on the phase of vegetation – it is approximately at the same level determined in the phases of vegetation and flowering phases (26.78% and 27.26%, respectively) and there is a decrease in the fruit phase (18.17%). The main monounsaturated fatty acid is 18:1n9. Its greatest content was found in the fruiting phase, the smallest – flowering. The predominant polyunsaturated fatty acid is 18:2n9,12. Its greatest content was found in the vegetation phase (26.78%), the lowest - flowering (24.23%). However, the total content of unsaturated fatty acids exceeds the total content of saturated fatty acids in all phases of plant development. Although, their ratio varies from phenophase. There is a general pattern of highest content of phenolic compounds in the flowering phase for all studied samples. It was shown that during the flowering phase, the maximum accumulation of essential oils, lipids and phenolic compounds is observed.

Refining high-quality leaf protein and valuable co-products from green biomass of Jerusalem artichoke (Helianthus tuberosus L.) for sustainable protein supply

The present study evaluates the green biomass of Jerusalem artichoke (JA) as an alternative green protein. A leaf protein concentrate (LPC) was prepared from leafy shoots using biotechnological methods. Seven clones were compared to assess the importance of the genetic basis of JA, and alfalfa served as the control. The LPC content of JA was an average 39 g kg−1 of fresh biomass, while that of alfalfa was 32 g kg−1. The JA can produce up to 936 kg of protein ha−1 year−1 without fertilization under rainfed conditions. The crude protein content of the LPC varied from 24.2 to 31.4 m/m%, depending on clones and harvesting time, which was comparable to that of alfalfa LPC (~ 32.3%). The amino acid profile of the LPC of JA, particularly of the essential amino acids, was similar to alfalfa and soybean. In addition, our results confirmed that the polyunsaturated fatty acid (PUFA) content varied between 64 and 68% in the LPC fraction, regardless of the clone that was used, with linoleic acid and linolenic acid being the predominant PUFAs. In addition, unlike alfalfa, the content of arachidonic acid was 0.5% in the JA LPC. The tuber yield was significantly reduced because of the repeated harvesting of the shoot parts; however, the tubers obtained were sufficient to regenerate the plantation in the subsequent year, thus ensuring the renewable ability and sustainability of the green biomass of JA.

Changes of fatty acid profile of mullet fish (Mugil cephalus) fillets as influenced by gamma irradiation

The current study was conducted to assess fatty acids profile of Mugil cephalus fillets as affected by low-dose of commercial Gamma ray’s irradiation (0, 0.75, 1.5, 2.25 and 3 kGy). The results showed that the palmitic (c16:0) and stearic acids (c18:0) were predominant saturated fatty acids (SFAs) while, oleic (c18:1) and linoleic acids (c18:2) were the predominant unsaturated fatty acids (USFAs) in all treated and control samples. The concentration of total saturated (SFAs) and monounsaturated fatty acids (MUFAs) were incremented with increment irradiation dose. Oleic acid (c18:1) and Palmitoleic acid (c16:1) were major (MUFAs) in all treated and control samples of Mugil cephalus. Linoleic (c18:2), eicosapentaenoic (c20:5) and docosahexaenoic (c22:6) acids were predominant polyunsaturated fatty acid (PUFAs) regarding treated in addition to control samples. The Significant difference (p < 0.0 5) was found between irradiated and non-irradiated Mugil cephalus of some PUFAs (Linoleic, Arachidonic and Eicosapentaenoic acid).

Effect of Omega-3 Fatty Acid Supplementation on Oxylipins in a Routine Clinical Setting.

Omega-6 polyunsaturated fatty acid (n-6 PUFA) is the predominant polyunsaturated fatty acid (PUFA), especially in Western diet. A high omega-6/omega-3 ratio in Western diets is implicated in the development of cardiovascular diseases and inflammatory processes. Studies in animal models and in humans have demonstrated beneficial effects of omega-3 PUFA (n-3 PUFA) in a variety of diseases, including cardiac arrhythmias and inflammatory diseases, as well as breast and colon cancer. The molecular mechanisms underlying the effects of n-3 PUFA are still not well understood. Possible mechanisms include competition between n-3 and n-6 PUFAs at the cyclooxygenase (COX) and lipoxygenase (LOX) and cytochrome P450 levels, and subsequent formation of oxylipins with specific anti-inflammatory or anti-arrhythmic effects. In this study, we report the impact of routine long-term treatment with prescription-grade n-3 PUFA (either 840 mg or 1680 mg per day) on blood cell membrane fatty acid composition, as well as plasma oxylipin patterns, in a patient population with severe hyperlipidemia and cardiovascular disease who are on standard lipid-lowering and cardioprotective medications. Lipidomics analyses were performed by LC/ESI-MS/MS. Supplementation led to a dose-dependent increase in n-3 PUFA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the blood cell fraction. We also observed a dose-dependent increase in EPA- and DHA-derived epoxy metabolites, whereas the effect of n-3 PUFA supplementation on LOX-dependent EPA- and DHA-derived hydroxy metabolites was less pronounced, with a tendency towards lower metabolites in subjects with higher n-3 PUFA levels. These data thus generally confirm effects of n-3 PUFA supplementation observed previously in healthy individuals. Additionally, they indicate a suppressive effect of high n-3 PUFA supplementation on the formation of LOX metabolites in the context of concomitant aspirin medication.

Comparative study of the nutritional composition and toxic elements of farmed and wild Chanodichthys mongolicus

Information of the difference in quality between farmed and wild fish is central to better ensuring fish products produced in aquaculture meet regulatory and consumer requirements. Proximate composition, amino acid and fatty acid profiles, and toxic elements contents of farmed and wild Chanodichthys mongolicus were established and compared. Significantly higher crude protein content while lower moisture content in farmed fish compared to wild fish were observed (P<0.05). The percentages of total amino acids (TAA), total essential amino acids (TEAA), total non-essential amino acids (TNEAA) and total delicious amino acids (TDAA) in farmed fish were all significantly higher than those in the wild equivalent (P<0.05). Compared to the FAO/WHO recommended reference values, the ratios of TEAA/TAA (39.84%–40.33%) were comparable to 40% and TEAA / TNEAA (66.22%–67.60%) were above 60%. Fatty acid profiles in both farmed and wild C. mongolicus were dominated by monounsaturated fatty acid (MUFA), with farmed fish contained much more MUFA content compared to wild counterpart (P<0.05). Notably, wild fish exhibited significantly higher levels of total polyunsaturated fatty acid (PUFA) including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) than farmed fish (P<0.05). The EPA (C20:5n3) and linoleic acid (C18:2n6) were the predominant PUFA in wild and farmed C. mongolicus, respectively. Moreover, farmed fish displayed an overall lower toxic element levels (As, Cd, Pb and Hg) in comparison with wild fish, and both were far lower than the established limit standard. In conclusion, our results suggest that the nutritional quality of farmed C. mongolicus was inferior to their wild counterpart with respect to fatty acids nutrition, and therefore further studies should focus on the improving C. mongolicus diet in order to enhance the overall nutritional composition.

Abstract 4312: Preneoplastic activity of dietary poly unsaturated fatty acid (PUFA) regulation of organ and tissue microenvironments in an iso-caloric pair-fed mouse model

Abstract Diets containing omega-3 (ω3) PUFAs have health benefits due to their anti-inflammatory activity and lower risk of chronic conditions including cardiac, autoimmune and neoplastic diseases. This contrasts with ω6 PUFA containing Western diets, which are pro-inflammatory. Balancing the dietary ω6:ω3 ratio has been suggested to have cancer preventive and potentially therapeutic activity; however, the majority of these studies have not differentiated dietary PUFA content from obesity. Herein we examined the effects of the ω6:ω3 ratio in iso-caloric diets that were pair fed. These diets had 35.5% of calories from fat with an ω6:ω3 ratio in the ω6 and ω3 based diets of 42:1 and 1:1 respectively (confirmed by gas chromatography-mass spectrometry) using the liquid, Lieber DeCarli diet and fish oil substituting for 70% of olive oil. The ω3 diet contained eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid at a 3:1 ratio, while the ω6 diet contained linoleic acid as the predominant ω6 PUFA and a low level of the ω3 PUFA, linolenic acid. After establishing female BALB/c mice on these diets, weight gain and diet consumption were monitored for 12 weeks, the mice sacrificed and the dietary effects on mammary gland and hepatic histopathology, leukocyte phenotypes and organ and tissue lipidomics determined. In association with pair feeding there were no differences in diet consumed (ω-3 diet used as baseline) and weight gain between cohorts. The cohort on the ω6 diet had depressed numbers of marrow progenitor cells and increased splenic subcapsular extramedullary hematopoiesis consistent with an inflammatory response, increased hepatocyte lipidosis, hypertrophy and multinucleation and increased hepatic vascularity with thicker intima. The ω-6 dietary cohort also had mammary fat pads (MFPs) with increased adipocytes in the tubular epithelium, stromal cellularity and epithelial tissue density. These mammary gland and hepatic histopathologic changes and subclinical inflammation are consistent with pre-neoplastic lesions and were accompanied by organ specific lipodemic changes. This included significant increases in arachidonic acid (AA) in the plasma, spleen and liver, but not MFPs, of the ω-6 cohort, and a significant increase in EPA and DHA levels in the plasma, spleen, MFPs and liver of the ω-3 cohort. In addition, the liver and MFPs had a significant increase in the ω3 PUFA, docosapentaenoic acid. In summary, our studies demonstrate that the dietary ratio of ω6:ω3, independent of obesity can regulate mammary gland and hepatocyte proliferation and subclinical inflammation in association with significant, organ specific increases in AA levels contributing to microenvironmental preneoplastic hyperplasia. Citation Format: Saraswoti Khadge, Paul Black, Concetta DiRusso, Geoff Thiele, Lynell W. Klassen, J Graham Sharp, Timothy R. McGuire, Michael J. Duryee, Holly C. Britton, Alicia Dafferner, Jordan Beck, James E. Talmadge. Preneoplastic activity of dietary poly unsaturated fatty acid (PUFA) regulation of organ and tissue microenvironments in an iso-caloric pair-fed mouse model. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 4312.

Physicochemical properties, antioxidant activities and protective effect against acute ethanol-induced hepatic injury in mice of foxtail millet (Setaria italica) bran oil.

This study was designed to investigate physicochemical characterization of the oil extracted from foxtail millet bran (FMBO), and the antioxidant and hepatoprotective effects against acute ethanol-induced hepatic injury in mice. GC-MS analysis revealed that unsaturated fatty acids (UFAs) account for 83.76% of the total fatty acids; in particular, the linoleic acid (C18:2) is the predominant polyunsaturated fatty acid (PUFA), and the compounds of squalene and six phytosterols (or phytostanols) were identified in unsaponifiable matter of FMBO. The antioxidant activity examination of FMBO in vitro showed highly ferric-reducing antioxidant power and scavenging effects against DPPH· and HO· radicals. Furthermore, the protective effect of FMBO against acute hepatic injuries induced by ethanol was verified in mice. In this, intragastric administration with different dosages of FMBO in mice ahead of acute ethanol administration could observably antagonize the ethanol-induced increases in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and the hepatic malondialdehyde (MDA) levels, respectively, along with enhanced hepatic superoxide dismutase (SOD) levels relative to the control. Hepatic histological changes were also observed and confirmed that FMBO is capable of attenuating ethanol-induced hepatic injury.

A Protocol for Quantifying Lipid Peroxidation in Cellular Systems by F2-Isoprostane Analysis

Cellular systems are essential model systems to study reactive oxygen species and oxidative damage but there are widely accepted technical difficulties with available methods for quantifying endogenous oxidative damage in these systems. Here we present a stable isotope dilution UPLC-MS/MS protocol for measuring F2-isoprostanes as accurate markers for endogenous oxidative damage in cellular systems. F2-isoprostanes are chemically stable prostaglandin-like lipid peroxidation products of arachidonic acid, the predominant polyunsaturated fatty acid in mammalian cells. This approach is rapid and highly sensitive, allowing for the absolute quantification of endogenous lipid peroxidation in as little as ten thousand cells as well as damage originating from multiple ROS sources. Furthermore, differences in the endogenous cellular redox state induced by transcriptional regulation of ROS scavenging enzymes were detected by following this protocol. Finally we showed that the F2-isoprostane 5-iPF2α-VI is a metabolically stable end product, which is excreted from cells. Overall, this protocol enables accurate, specific and sensitive quantification of endogenous lipid peroxidation in cellular systems.

Chemical and fatty acid composition of male mouflon (Ovis ammon musimon Pal.) meat

The aim of this research was to determine the effect of age on chemical and fatty acid composition of the male mouflon (Ovis ammon musimon Pal.) meat. The male mouflons included in the study originated from the North Adriatic island Rab, Croatia. Three age groups were included (young, sub-adult, adult). An effect of age on chemical composition was not found. However, higher percentage of total fat, dry matter and ash was determined in the meat of young mouflons. Predominating saturated fatty acids in mouflon meat were myristic, palmitic and stearic acids. Age significantly (p < 0.05) affected only the percentage of palmitic acid being the highest in the meat of young mouflons. Percentage of the predominant monounsaturated fatty acid, oleic acid, did not change significantly with age. A higher (p < 0.05) percentage of the predominant polyunsaturated fatty acid, linoleic acid, was found in the meat of sub-adult mouflons. The meat of the young mouflons had a higher (p < 0.05) percentage of conjugated linoleic acid. The meat of the sub-adult and adult mouflons was found to have a higher (p < 0.05) percentage of total saturated fatty acids. A lower (p < 0.05) ratio of polyunsaturated and saturated fatty acids was found in the meat of young than in the meat of sub-adult mouflons. The ratio of n-6 and n-3 was lower (p < 0.05) in the adult than in the sub-adult mouflons. Higher (p < 0.05) atherogenicity and thrombogenicity indices were found in the young rather than in the adult mouflons. Low fat content (<1 %) and favourable ratios of polyunsaturated, saturated, n-6 and n-3 fatty acids make the mouflon meat a good choice for human diet.

Fatty acid composition and Ω3/Ω6 ratios of the muscle lipids of six fish species in Sugla Lake, Turkey

Fatty acid composition of the muscle lipids of Carassius gibelio, Pseudophoxinus anatolicus, Sander lucioperca, Tinca tinca, Vimba vimba tenella and Capoeta capoeta in Sugla Lake were determined. In all species, palmitic acid (13.25- 18.54% of total fatty acids) and oleic acid (11.93-34.23% of total fatty acids) were identified as major saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA), respectively. Docosahexaenoic acid (DHA) was found to be the major polyunsaturated fatty acid (PUFA) in T. tinca, C. capoeta, C. gibelio, P. anatolicus and S. lucioperca while the predominant PUFA of V. vimba tenella was eicosapentaenoic acid (EPA). S. lucioperca contained more ?3 fatty acids than the other fish species. The percentages of total ?3 fatty acids were higher than those of total ?6 fatty acids in all species. Since P. anatolicus is endemic and endangered, this species should be protected and produced for future marketing.

Comparative fatty acid profiles of wild and farmed tropical freshwater fish rohu (Labeo rohita)

The proximate composition of the whole body and the fatty acid composition of the liver, muscle, eye and brain of wild and cultured rohu (Labeo rohita) were analyzed. The cultured species was found to have significantly (P < 0.05) higher lipid contents than its wild counterpart. The saturated (SFA) and monounsaturated (MUFA) fatty acid contents were significantly higher in the cultured species, whereas the n-6 and n-3 polyunsaturated fatty acid (PUFA) levels were higher in the wild species. Fatty acids C16:0 and C18:1 n-9 were the principal fatty acids of the SFAs and MUFAs, respectively, identified in the analyses. Docosahexaenoic acid, eicosapentaenoic acid, and arachidonic acid were the predominant PUFAs in both groups, and all three were found to be present at significantly (P < 0.05) higher levels in the wild species. Erucic acid (C22:1 n-9), which was the predominant fatty acid (30.76%) in the feed, was detected only at low levels in muscle (0.30%), liver (1.04%) and eye (1.28%) of cultured fish tissue.

A Comparison of Proximate Composition And Fatty Acid Profile of Indus River Fish Species

Eight species of freshwater fish from the Indus River were analyzed for their proximate composition and fatty acid (FA) profile. Differences were observed (P < 0.05) for moisture (59.95–79.45%), ash (0.05–4.95%), crude protein (17–20.09%) and lipid (0.85–18.32%) contents. The changes in FA profiles of fish species were significant (P < 0.05). The monounsaturated (MUFA) fatty acid content (24.55–48.35 g/100 g) was higher than the saturated fatty acid (SFA) (25.04–41.02 g/100 g) and polyunsaturated fatty acid (PUFA) (15.72–35.34 g/100g). The predominant PUFAs were eicosapentaenoic acid (C20:5 n-3) and docosahexaenoic acid (C22:6 n-3). All of the fish species had n-3/n-6 within the recommended values (1–4), which confirms he importance of River Indus fish as a significant dietary source of n-3 PUFA.

Innovations in the development of healthier chicken sausages formulated with different lipid sources

Long-chain polyunsaturated n-3 fatty acids are critical nutrients for human health and the fortification of foods with these fatty acids is an important emerging area from the commercial and academic point of view. Development, characterization, and changes during refrigerated vacuum storage of low-fat chicken sausages formulated with preemulsified squid oil were examined and compared with those formulated with beef tallow. Physicochemical analysis and process yield after heat treatment were determined; the heat-treated sausages were evaluated by purge loss, color, texture, microstructure by SEM, microbial counts, fatty acid profile, lipid oxidation, and sensory analysis during refrigerated vacuum storage. Process yield of both formulations was higher than 97% and purge losses during storage were lower than 7%. Purge losses of oil-formulated sausages were lower than those with beef tallow. Sausages with squid oil resulted in higher lightness, lower redness and yellowness, and lower texture profile analysis parameters than the formulation prepared with beef tallow. Microstructure of both formulations was similar, except for the fat droplets that microscopic observations showed in the sausages made with beef tallow. Low lipid oxidation was detected in formulation with squid oil due to the the combination of ingredients and storage conditions. Microbial counts of both products were less than 5 log cfu/g at the end of 90 d of storage. The sausage formulated with squid oil presented more than 30 and 40 g/100 g of monounsaturated and polyunsaturated fatty acids, respectively. Docosahexaenoic acid was the predominant polyunsaturated fatty acid, followed by eicosapentaenoic acid and linoleic acid. Both products showed safe sanitary conditions, good sensory acceptability, and presented very good stability and quality attributes, but sausages formulated with squid oil showed a better fatty acid profile according to nutritional criteria.

Composición de ácidos grasos de los caracoles marinos Phyllonotus pomum y Chicoreus brevifrons (Gastropoda: Muricidae)

Muricid species of P. pomum and C. brevifrons are of economic importance in the Caribbean. This study includes a comparative evaluation of fatty acid content in the total lipid composition of Phyllonotus pomum and Chicoreus brevifrons. Snail samples were collected during the rainy, dry and transition seasons, in Punta Arena, Sucre (Venezuela). Total lipids were extracted and the specific fatty acid contents were analyzed by gas chromatography. Lipid concentrations varied between 0.87 and 1.85%, with minimum and maximum values corresponding to C. brevifrons collected during rainy and dry seasons, respectively. In the case of total lipids, a high concentration of unsaturated fatty acids (57.21-70.05%) was observed followed by saturated fatty acids (20.33-31.94%), during all seasons. The polyunsaturated occurred in higher proportion among the unsaturated fatty acids, except for P. pomum which showed higher proportion of monounsaturated fatty acids (38.95%) during the transition season. The prevailing fatty acids were: C14:0, C16:0, C18:0, C20:1, C22:1 omega-11, C22:1 omega-9, C18:3 omega-3, C20:5 omega-3 and C22:6 omega-3, among which docosahexaenoic acid was the predominant polyunsaturated fatty acid, showing values between 4.62 and 33.11%. The presence of high concentrations of polyunsaturated fatty acids found in P. Pomum and C. brevifrons allow their recommendation for human consumption with appropriate resource utilization.

Cytochrome P450-dependent metabolism of eicosapentaenoic acid in the nematode Caenorhabditis elegans

The genome of Caenorhabditis elegans contains 75 full length cytochrome P450 (CYP) genes whose individual functions are largely unknown yet. We tested the hypothesis that some of them may be involved in the metabolism of eicosapentaenoic acid (EPA), the predominant polyunsaturated fatty acid of this nematode. Microsomes isolated from adult worms contained spectrally active CYP proteins and showed NADPH-CYP reductase (CPR) activities. They metabolized EPA and with lower activity also arachidonic acid (AA) to specific sets of regioisomeric epoxy- and ω-/(ω-1)-hydroxy-derivatives. 17( R),18( S)-epoxyeicosatetraenoic acid was produced as the main EPA metabolite with an enantiomeric purity of 72%. The epoxygenase and hydroxylase reactions were NADPH-dependent, required the functional expression of the CPR-encoding emb-8 gene, and were inhibited by 17-ODYA and PPOH, two compounds known to inactivate mammalian AA-metabolizing CYP isoforms. Multiple followed by single RNAi gene silencing experiments identified CYP-29A3 and CYP-33E2 as the major isoforms contributing to EPA metabolism in C. elegans. Liquid chromatography/mass spectrometry revealed that regioisomeric epoxy- and hydroxy-derivatives of EPA and AA are endogenous constituents of C. elegans. The endogenous EPA metabolite levels were increased by treating the worms with fenofibrate, which also induced the microsomal epoxygenase and hydroxylase activities. These results demonstrate for the first time that C. elegans shares with mammals the capacity to produce CYP-dependent eicosanoids and may thus facilitate future studies on the mechanisms of action of this important class of signaling molecules.