1426 PURPOSE: This study cross-validated the 20-meter shuttle run test (20 MST) in predicting VO2 max values of college soccer athletes. METHODS: Forty-nine male college soccer players (age 21.62 ± 0.95 yrs) completed VO2 max evaluations via open circuit spirometry and maximal treadmill testing (TMT) and the 20 MST. Shuttle VO2 max was calculated from the following regression equation: VO2 max = −27.4 + 6.0 * maximal running speed (Mercier et al. 1983). RESULTS: The following cross-validation statistics were computed: r = 0.79 and SEE = 4.03 mlkg−1.min−1. The following mean and SD values for the respective actual and predicted VO2 max values were: (TMT = 56.04 ± 6.54 mlkg−1.min−1 and 20 MST = 55.56 ± 5.36 mlkg−1.min−1). A paired t-test indicated no significant difference between the TMT and 20 MST, P = 0.41. The Bland-Altman statistics indicate that the limits of agreement (95% confidence intervals) were −7.48 to 8.43 mlkg−1.min−1). CONCLUSIONS: Results of this investigation found the 20 MST to be an appropriate predictor of VO2 max and was suitable as a field test to predict VO2 max values of college soccer athletes.