Pre-sprouted Sugarcane Seedlings Research Articles

Ferronickel mining slag on substrates for production of pre-sprouted sugarcane seedlings

Ferronickel mining slag on substrates for production of pre-sprouted sugarcane seedlings

Efeitos da inoculação de Nitrospirillum amazonense e biofertilizante em cana-de-açúcar

ABSTRACT: In this study, we analyzed the hypothesis that the combination of Nitrospirillum amazonense strain BR11145 with biological fertilizer prepared using Microgeo® and native microbiome from location of the product application results in morphological and nutritional gains for the initial development of sugarcane plants and soil chemistry. For this purpose, pre-sprouted sugarcane seedlings were grown in a greenhouse mesocosm experiment using soil amended with nitrogen/phosphorus/potassium fertilizer. The experimental treatments consisted of: 1) biological fertilizer with the addition of N. amazonense (100 mL ha-1), 2) biological fertilizer without the addition of N. amazonense, 3) inoculation with N. amazonense at a dose of 100 mL ha-1 with 2x108 viable cell mL-1, 4) inoculation with N. amazonense at a dose of 200 mL ha-1 with 2x108 viable cell mL-1, and 5) control, without the addition of biological fertilizer and N. amazonense. The biological fertilizer was applied at dose of 300 L ha-1, which was split at planting (200 L ha-1) and in the post-emergence phase (100 L ha-1). After 164 days of planting, it was detected an increase in leaf length +3, number of green leaves, leaf area and sulfur content in the leaves of sugarcane plants that received the biological fertilizer with the addition of N. amazonense. In conclusion, the combination of N. amazonense with biological fertilizer revealed positive effects through morphological and nutritional characteristics in sugarcane plants during their early stages of development when compared to plants grown only with the inoculation of N. amazonense, biological fertilizer or mineral fertilizers, with few notable positive effects on soil chemistry.

Biochar influences the cane fields’ microbiota and the development of pre-sprouted sugarcane seedlings

Biochar influences the cane fields’ microbiota and the development of pre-sprouted sugarcane seedlings

Weed interference periods in pre-sprouted sugarcane seedlings

The aim of this study was to determine the period prior to interference (PPI), the critical-period of interference prevention (CPIP), and the total-period of interference prevention (TPIP) in sugarcane in the pre-sprouted seedlings (PSS) system, in coexistence with Cyperus rotundus and other weeds, adopting a loss of 5% in yield. The treatments corresponded to seven coexistence periods and seven weed control periods: 0–30, 0–60, 0–90, 0–120, 0–150, 0–180, and 0–240 days after planting (DAP). The cultivar used was IACSP95-5000. Until 26 DAP, weeds did not compete with sugarcane. From 26 to 68 DAP, weeds competed with sugarcane. Thus, it is considered that from 68 DAP, weed control is no longer necessary because increased periods of control do not result in significant yield gains. The results showed a 26 days PPI, 26–68 days CPIP, and 68 days TPIP, adopting a loss of 5% in yield, for weed competition with sugarcane in the PSS system. There was a reduction of 8.22% in sugarcane yield per day of coexistence and an increase of 11.37% per day of control.

Micrometeorological Modification Promoted by Photoselective Meshes and Supplementary Lighting in the Production of Pre-sprouted Sugarcane Seedlings

Micrometeorological Modification Promoted by Photoselective Meshes and Supplementary Lighting in the Production of Pre-sprouted Sugarcane Seedlings

Quality of pre-sprouted sugarcane seedlings grown on substrates with decomposed babassu palm stem

ABSTRACT The use of pre-sprouted seedlings in the implantation of sugarcane crops not only reduces the establishment period and production costs, but also has other advantages, such as a lesser requirement of propagules (stems) and greater phytosanitary control of the seedlings, when compared to conventional planting. Commercial substrates are also expensive and their use increases the cost of seedlings. This study aimed to evaluate the quality of sugarcane pre-sprouted seedlings grown on substrates with different proportions of decomposed babassu palm stem (DBPS), as well as to recommend the substrate formulation that provides the most favorable environment for the growth and development of sugarcane pre-sprouted seedlings. For preparation of the substrate (S), DBPS and soil were used in the following proportions: S1: 0 % of DBPS + 100 % of soil; S2: 20 % of DBPS + 80 % of soil; S3: 40 % of DBPS + 60 % of soil; S4: 60 % of DBPS + 40 % of soil; S5: 80 % of DBPS + 20 % of soil; S6: 100 % of DBPS + 0 % of soil. The use of S2 and S3 considerably improved the seedling quality parameters, such as sprout diameter and length, number of leaves and total, shoot and root dry mass. However, from an economic point of view, S2 is recommended for use.

Root- and foliar-applied silicon modifies C: N: P ratio and increases the nutritional efficiency of pre-sprouted sugarcane seedlings under water deficit.

Water deficit limits the establishment of sugarcane plants from pre-sprouted seedlings (PSS). Silicon (Si) can mitigate such stress, and your supply in plants with the active absorption mechanism is carried out through roots. However, foliar spraying has been practiced to supply Si in PSS production nurseries. Althought it is known that Si via roots can alter C: N: P ratios, nothing has been reported about its supply via foliar spraying, nor whether such changes interfere with structural nutrient use efficiency and with plant physiological responses. Thus, this study aimes to asses whether Si foliar spraying changes C: N: P ratios and increases the nutritional efficiency of PSS, as well as whether water deficiency interferes with such a relationships. For these purposes, three experiments were carried out. In experiment I, treatments consisted of two sugarcane cultivars (CTC 4 and RB 966928) and three Si supply forms (in nutrient solution via roots [SiR], via foliar spraying [SiL], and one control with the absence of Si [-Si]). The same Si supply forms were used in the other two experiments. In experiment II, a short-term water deficit was induced by polyethylene glycol addition to nutrient solution (-0.6 MPa) for three days. In experiment III, a long-term water deficit was imposed using levels of soil water retention capacity (70% [no water deficit], 50% [moderate water deficit], and 30% [severe water deficit] for 30 days. Our findings revealed that Si supply decreased C concentrations regardless of water conditions and that N and P concentrations varied with Si supply form and water deficit level. Moreover, root- and foliar-applied Si modified the C: N: P stoichiometry and increased C use efficiency in PSS, which thus increased N and P use efficiencies. Such an increased nutritional performance helped adjust physiological parameters and increase dry matter yield in PSS, both under water stress and non-stress conditions. Further, Si foliar spraying promoted structural effects on PSS regardless of water conditions, even if sugarcane has an active absorption via roots. In conclusion, foliar spraying can be used to supply Si in PSS production nurseries.

Immunity of sugarcane cultivars to Meloidogyne enterolobii

Brazil is currently the world’s largest producer and exporter of sugarcane, and the crop has high socioeconomic importance in the country. Root-knot nematodes (Meloidogyne spp.) are one of the major limiting factors in sugarcane production. These plant parasites have wide geographic distribution, high damage potential, and are difficult to control. Recently, the species Meloidogyne enterolobii was identified in sugarcane crops in the state of Pernambuco, Brazil. Given the importance of genetic resistance for integrated nematode management and the lack of research on the M. enterolobii–sugarcane pathosystem, this study aimed to assess the response of sugarcane cultivars to M. enterolobii. Thirteen cultivars were evaluated for their resistance to M. enterolobii based on the nematode reproduction factor. The experiment was conducted in a greenhouse, in a completely randomized design, with 14 replicates. Pre-sprouted sugarcane seedlings were transplanted to 5 L pots, and each pot was considered an experimental unit. At 15 days after transplanting, the seedlings were inoculated with 5,000 eggs and second-stage juveniles of M. enterolobii. Tomato and okra plants were also inoculated to test the viability of the inoculum. At 240 days after inoculation, plant roots were processed and evaluated for nematode number. This parameter was used to calculate the nematode reproduction factor on each cultivar. All sugarcane cultivars were found to be immune to M. enterolobii, with a reproduction factor of 0.

Desenvolvimento de variedades de cana-de-açúcar sob aplicação foliar de Nitrogênio

A cana-de-açúcar tem grande importância socioeconômica, devido a geração de emprego, e por ser matéria prima para diversos produtos. Portanto, é imprescidível realizar o manejo nutricional adequado, principalmente do nitrogênio (N), visando beneficiar a produtividade da cultura. O objetivo do trabalho foi avaliar o desenvolvimento de diferentes variedades de cana-de-açúcar cultivadas com fornecimento de N via solução nutritiva e foliar. O experimento foi realizado em casa de vegetação do Instituto Federal de Pernambuco - Campus Vitória, utilizando mudas pré-brotadas de cana-de-açúcar (MPB), em delineamento em blocos casualizado, com duas variedades RB992506 e CTC961007, cultivadas em areia lavada na ausência e presença de N, com dois níveis de aplicação foliar do N (com e sem), arranjo fatorial (2 x 2 x 2), com três repetições. Aos 53 DAT foram realizadas as avaliações: diâmetro do colmo; altura da planta; massa fresca e seca da parte aérea e da raiz; clorofila; largura e comprimento, da folha +1. A solução nutritiva completa, incrementou todas as variáveis supracitada, independentemente da aplicão foliar de N. De modo geral a aplicação foliar, só apresentou incremento, quando associada a solução incompleta. O nitrogênio proporcionou melhor desenvovimento e maior produtividade da cultura.

Nitrogênio e Azospirillum brasilense no desenvolvimento inicial da cana-de-açúcar

A study was conducted with the objective of investigating the effects of the interaction between the inoculation of the pre-sprouted seedlings with Azospirillum brasilenseand the nitrogen fertilization in topdressing on the development of sugarcane plants(Saccharum officinarum). Pre-sprouted sugarcane seedlings, produced from 3.0 cm long mini-stems of the RB867515 variety, were transplanted 25 days after sprouting into 12 L plastic pots. The treatments were arranged in a randomized block design, in a 2 × 5 factorial scheme: two inoculation treatments [without (control) and inoculation with Azospirillum brasilense] and five application rates of N fertilizer in topdressing (0, 30, 60, 90 and 120 mg dm–3of N), with four repetitions. The inoculation with A. brasilensewas carried out in transplanting the seedlings using 4 mL of the inoculum AzoTotal® per seedling, containing the strains AbV5 and AbV6. The nitrogen fertilization in topdressing was performed at 20 days after transplanting the seedlings as urea (45% of N). The results reported that the application of N fertilizer rates in topdressingresulted in a linear increase in plant height, stem diameter, number of leaves per plant, number of tillers and dry matter production of plants inoculated with A. brasilense.When the plants were not inoculated with A. brasilense, nitrogen fertilization resulted in an increase also in the number of leaves and tillers per plant. The use of A. brasilenseinoculation in pre-sprouted sugarcane seedlings has a beneficial effect on the development of plants only when associated with nitrogen fertilization in topdressing. Sugarcane plants not fertilized with N have a negative response to inoculation with A. brasilense, indicating the occurrence of possible phytotoxic effects on plant development up to 60 days after transplanting.

Response of Pre-sprouted Sugarcane Seedlings to Foliar Spraying of Potassium Silicate, Sodium and Potassium Silicate, Nanosilica and Monosilicic Acid

Silicon (Si) applied via foliar on pre-sprouted sugarcane seedlings is a practical alternative for supplying Si, however, it is necessary to use an adequate Si source and optimal concentration, but this information is unknown. This study evaluates the effect of alternative Si sources applied via foliar spraying at lower concentrations in relation to potassium silicate, on Si accumulation and the beneficial effects on pre-sprouted sugarcane seedlings and initial growth. Two experiments were carried out in a greenhouse, one in the production phase of pre-sprouted seedlings and the other in the initial growth phase of sugarcane grown in pots. Both experimental designs were randomized blocks with treatments arranged in a 4 × 5 factorial scheme, with four Si sources: nanosilica (Si-nano), monosilicic acid stabilized with PEG-400 (Si-acid), sodium and potassium silicate stabilized with sorbitol (Si-alkali) and potassium silicate (Si-K) in five concentrations (0, 0.25, 0.5, 0.75 and 1.0 mmol L−1), and 4 repetitions. The Si syrup pH was adjusted to 5.5 ± 0.2. The foliar spraying of Si on pre-sprouted sugarcane seedlings is feasible since it increased Si accumulation, and benefited seedling production and plant initial growth. Soluble Si sources with stabilizers increased Si accumulation more efficiently compared to potassium silicate (Si-K) and nanosilica (Si-nano). Plants that have been sprayed with the PEG-400-stabilized monosilicic acid (Si-acid) and the sorbitol-stabilized sodium and potassium silicate (Si-alkali) at 0.5 and 0.6 mmol L−1 concentrations, respectively, promote had greater Si accumulation and beneficial effects in the growth of pre-sprouted sugarcane seedlings in production phase. In the initial growth of sugarcane, the sources Si-acid and S-alkali also increase the buildup and beneficial effects, but both sources at 0.6 mmol L−1 concentrations.

Growth of pre-sprouted sugarcane seedlings submitted to supplementary lighting

ABSTRACT Brazil is the largest producer and exporter of sugarcane in the world, and the sugar and ethanol sector has invested in the development of new technologies and methods of planting to meet the market demand. Thus, the aim of this study was to evaluate the performance of sugarcane at the acclimation phase and under field conditions, from pre-sprouted seedlings exposed to supplementary lighting, through light-emitting diode, in the period of acclimation in a greenhouse. The experiment was carried out in Carpina, PE, Brazil, from January to September 2017. The experimental plots were composed of pre-sprouted seedlings acclimated in greenhouse, exposed to the supplementary lighting systems, 90% Red + 10% Blue (R:B 90/10), 80% Red + 20% Blue (R:B 80/20), 70% Red + 30% Blue (R:B 70/30) and a control. The evaluation of seedling growth was performed through the measurement of biometric indicators: plant height (cm); stem length (cm); stem diameter (cm) and number of tillers. The biometric indicators showed no significant differences, but plants under lighting systems with a greater proportion of the red wavelength (R:B 80/20 and R:B 90/10) showed higher percentage of survival in the field.

EFFECTS OF PHOTOSELECTIVE SHADE NETS ON EARLY GROWTH OF SUGARCANE SEEDLINGS

ABSTRACT Sugarcane has a great economic interest in Brazil, so it needs technologies to increase its yield strength, especially for seedling production. This study aimed to ascertain the influence of shading nets on early growth and development of pre-sprouted sugarcane seedlings. An experiment was conducted in a protected environment, in the Rural Federal University of Pernambuco (UFRPE), in Recife-PE, Brazil. The protected environments were covered with anti-UV low-density polyethylene plastic plus: Solpack® red ultranet net, Solpack® white net, Solpack® freshnet net, and without shade net, and five replications each. The experiment was carried out in a completely randomized design, and multivariate principal component analysis (PCA) was used to verify associations among cultivation modules, micrometeorological factors, and seedling growth parameters. Anti-UV low-density polyethylene + shade net improved seedling growth and development up to 27 days after planting. Higher substrate temperatures benefited early growth of seedlings. After 27 days of planting, sugarcane seedlings required higher air temperatures, as well as increased global and photosynthetically active solar radiation.

Silicon in Pre-sprouted Sugarcane Seedlings Mitigates the Effects of Water Deficit After Transplanting

This study is aimed at assessing whether silicon (Si) can mitigate the effects caused by water stress in pre-sprouted seedlings (PSS) in sugarcane after their transplanting in the soil and what is the best form in the application if via nutrient solution or leaf spraying. The treatments were arranged in a 3 × 3 factorial scheme with silicon supply via nutrient solution (2 mmol L−1), Si via leaf application (3.4 mmol L−1), and without Si (control) and three levels of soil water retention capacity (70%, 50%, and 30%), for 30 days. In the absence of Si, the water deficit was damaging to the physiological, growth, and dry matter production variables of plants, in a 30% level of soil water retention capacity. Silicon supply via nutrient solution was more efficient when compared with Si via leaf spraying in the formation of pre-sprouted sugarcane seedlings in the first 30 days after transplanting, since it increased the quantum efficiency of photosystem II, chlorophyll content, leaf water potential, and water content, decreased cell electrolyte leakage and free proline content, and allowed a higher growth and biomass production. In conclusion, the supply of Si via nutrient solution in pre-sprouted sugarcane seedlings can be considered a feasible alternative to alleviate water deficit damage imposed after transplanting, in a 30% level of soil water retention capacity.

BIOPHOTONICS: ACCLIMATION OF PRE-SPROUTED SUGARCANE SEEDLINGS IN PROTECTED ENVIRONMENT

ABSTRACT Sugarcane is considered one of the main alternatives for biofuel production and an important product of Brazilian agriculture, so it is indispensable to adopt new techniques for its cultivation and management. Thus, the present study aimed to evaluate the effect of the quality of supplemental lighting, based on light-emitting diode (LED), on the acclimation of pre-sprouted sugarcane seedlings in a protected environment. The experimental design was completely randomized, consisting of three supplemental LED lighting systems, with light proportions of 90% red + 10% blue (R:B 90/10), 80% red + 20% blue (R:B 80/20) and 70% red + 30% blue (R:B 70/30), and a control, with natural solar radiation, with 5 replicates. Multivariate principal component analysis was used based on the matrix of correlation between all variables (meteorological, physiological and biometric). It was found that the different LED lighting systems increased the photosynthetically active radiation; however, until 20 days after planting, the seedlings were little influenced by the supplemental lighting systems. At the end of the acclimation period, the R:B 80/20 lighting system was the most associated with the growth and size of sugarcane seedlings.

Sprouting of pre-sprouted sugarcane seedlings and micrometeorological variables under photo-selective nets

ABSTRACT Sugarcane is a grass species that stands out worldwide in the production of ethanol. Brazil is the world’s largest producer and leader in exports, responsible for more than 50% of the products that are marketed worldwide. The objective of this study was to investigate the effect of photo-selective nets on micrometeorological variables and on sprouting of pre-sprouted sugarcane seedlings. The experiment was carried out in a protected environment at Universidade Federal Rural de Pernambuco - UFRPE, in a completely randomized design. Seedlings of cultivar RB92579 were obtained by the technique of production of pre-sprouted seedlings. The protected environment was divided into four modules corresponding to the treatments: covered with anti-UV low-density polyethylene plastic: + Solpack® red ultranet net, + Solpack® white net, + Solpack® freshnet net and without shade net. Micrometeorological data of air temperature and substrate temperature were recorded in each module. The first count of emergence, sprouting speed index and sprouting percentage were calculated. Principal component analysis was used to verify the association between the cultivation modules and the micrometeorological and sprouting variables of the seedlings. Air temperature in the protected environment was 8.7% higher than that in the external environment. The white net led to sprouting of 78.93%. The substrate temperature above 30.4 °C favored seedling sprouting. The modules with white net and red ultranet net favored seedling sprouting.

Development and Gas Exchange of Pre-sprouted Sugarcane Seedlings in Three Different Growing Substrate Media

Objective: Evaluate the development and gas exchange of pre-sprouted sugarcane seedlings in different substrates.
 Study Design: A randomized complete block design was used with two genotypes, three types of substrate (2 x 3), four replicates and five plants per replicate, totaling 120 plants.
 Location and Duration of Study: Department of Crop Sciences of the Center for Agrarian Sciences, Universidade Federal de Paraíba in the city of Areia, Brazil, between August and October 2018.
 Methods: The following were the treatments: T1 – genotype 1 in commercial substrate; T2 – genotype 1 in commercial substrate + bovine manure; T3 – genotype 1 in bovine manure + sand; T4 – genotype 2 in commercial substrate; T5 – genotype 2 in commercial substrate + bovine manure; T6 – genotype 2 in bovine manure + sand. Variables analyzed: height, diameter, dry mass of shoot and root and physiological response of seedlings.
 Results: A significant difference at the 1% level was found for the dry matter of the seedling roots among treatments with difference substrates. A significant difference at the 1% level was also found for shoot length and a significant interaction at the 5% level was found between genotype and substrate for this characteristic. Significant effects were found with regard to the rate of photosynthesis, stomatal conductance, transpiration and carboxylation efficiency.
 Conclusion: The substrate with manure + sand favored the accumulation of root dry matter and shoot length. Genotype 1 exhibited its best performance with regard to photosynthesis and stomatal conduction in the treatment with the commercial substrate and demonstrated greater carboxylation efficiency than genotype 2, independently of the substrates. The pre-sprouted seedling production system needs to be explored further to obtain greater information regarding the nutritional requirements and characteristics of the genotypes.

Production of sugarcane seedlings pre-sprouted in commercial and alternative substrates with by-products of the sugarcane industry

The sugarcane agro-industry is a major producer of organic waste, which could be used as substrates for production of pre-sprouted sugarcane seedlings aiming at reducing production costs and the sustainability of the sugar and alcohol industry. This study aimed to identify the best position of origin of the bud chip (little portion of stem with one bud) for the production of pre-sprouted seedlings (PSS) of sugarcane and the effects of commercial and alternative substrates containing by-products of the sugarcane industry on the production of sugarcane PSS. For this, a 4x2 factorial experiment was performed with four substrates: two commercial (TRIMIX® and BIOPLANT®) and two substrates formulated from sugarcane bagasse and filter cake (SABAFI and SUSBAFI) combined with bud chip from the basal and apical regions. The chemical and physical-hydric properties of the substrates and the development of PSS were evaluated. The substrates evaluated provided contrasting environments for root growth in relation to the physical-hydric and chemical properties. The use of by-products from the sugarcane industry can be added to the formulation of substrates for the production of pre-sprouted sugarcane seedlings, although the commercial substrate TRIMIX® provides superior performance to the seedlings, regardless of the origin position of the bud chip.

Weed Interference Periods in Pre-Sprouted Sugarcane Seedlings

ABSTRACT: The aim of this research was to determine weed interference periods in sugarcane cultivated under the system of pre-sprouted seedlings, taking into account the impact on the final plant stand, and bud yield and stalk yield. In the field, this study used a randomized block design with 14 treatments and four replications. The treatments consisted of periods of control and weed coexistence with the sugarcane crop (cultivar IACSP95-5000): 30, 60, 90, 120, 150, 180, 210 and 240 days after planting. A phytosociological survey was carried out in the experimental area, and the species Merremia aegyptia, Urochloa decumbens and Nicandra physaloides were found to be predominant. At the end of 240 days, regression analysis was performed according to Boltzmann’s sigmoidal model and considering loss tolerance of 5% in the evaluated variables. For final plant stand, the period prior to interference was 31 days and the total period of interference prevention was 187 days. For bud yield, the period prior to interference was 23 days and the total period of interference prevention was 178 days. For stalk yield, the period prior to interference was 19 days and the total period of interference prevention was 195 days. Weed interference was detrimental to all evaluated variables, with losses of up to 100% of the sugarcane plants after 120 days of weed coexistence.

DESENVOLVIMENTO DE MPB DE CANA DE AÇÚCAR EM FUNÇÃO DO USO DE NITROGÊNIO E TORTA DE FILTRO NO SOLO

Consideration should be given to the use of industrial waste as sources of fertilizers to soil and plants. The objective of this work was to study nitrogen rate as a function of filter cake doses in the initial development of pre-sprouted sugarcane seedlings. The was design completely randomized in a 4x3 factorial scheme, with 4 doses of filter cake and 3 doses of N, with 4 replicates. The variety used was CTC 9003. The evaluations occurred with 76 DAT (days after transplantation): number of tillers, height of plants, mass humid of the part aerial, total dry mass and root/shoot relationship. The use of filter cake at doses of up to 60 ton ha-1 combined with 100 kg ha-1 of N improves the initial development of sugarcane, being a viable alternative for the destination of this residue in the soil.