Objective: To compare indicators of fluid distribution and cellular integrity in university athletes through a cross-sectional study of 167 university athletes (18–35 years) who competed in team sports and individual sports. Approach: Bioimpedance was used to estimate total body water (TBW), intracellular water (ICW), extracellular water (ECW), ECW/ICW ratio, impedance (Z), reactance (Xc), resistance (R), phase angle, body cell mass (BCM) and ECW/BCM ratio at a frequency of 50 kHz. X-ray absorptiometry was used to determine lean soft tissue mass and body fat. As covariables, sex, age, time of practice and type of modality were obtained by questionnaire. Main results: Individual male athletes presented higher values of ECW/ICW ratio (means difference MD = 0.006, p = 0.020) and ECW/BCM ratio (MD = 0.006, p = 0.017) than team sports male athletes. Values of Z (MD = 24.988, p = 0.009), Xc (MD = 3.327, p = 0.008) and R (MD = 24.755, p = 0.09) were higher in male athletes practicing team sports than individual sports. Female team sports athletes presented higher values of Z (MD = 32.029, p = 0.015) and R (MD = 31.987, p = 0.015) than individual female athletes. It was concluded that ECW/ICW and ECW/BCM ratios were higher in male athletes who practiced individual sports. For the Z, R, and Xc values, the athletes in team sports presented higher values. The female athletes who practiced team sports presented higher values of Z and R than did individual female athletes. Significance: ECW/ICW and ECW/BCM ratios were higher in male athletes who practiced individual sports. For the Z, R, and Xc values, the athletes in team sports presented higher values. The female athletes who practiced team sports presented higher values of Z and R than did individual female athletes.