Practical Salinity Units Research Articles

Thermophilic aerobic digestion using aquaculture sludge from rainbow trout aquaculture facilities: effect of salinity

The objectives of this study were to evaluate the potential of using thermophilic aerobic digestion (TAD) to hydrolyze aquaculture sludge, and to investigate the hydrolysis efficiency and changes in microbial community structure during TAD at 0, 15, and 30 practical salinity units (psu). As digestion progressed, soluble organic matter concentrations in all reactors increased to their maximum values at 6 h. The hydrolysis efficiency at 6 h decreased as salinity increased: 2.42% at 0 psu, 1.78% at 15 psu, and 1.04% at 30 psu. The microbial community compositions at the genus level prominently differed in the relative abundances of dominant bacteria between 0 psu and 30 psu. The relative abundance of genera Iodidimonas and Tepidiphilus increased significantly as salinity increased. Increase in the salinity at which thermophilic aerobic digestion of aquaculture sludge was conducted altered the microbial community structure, which in turn decreased the efficiency of organic matter hydrolysis.

Monitoring the Amazon River plume from satellite observations

ABSTRACT Most of our knowledge about the Amazon River plume is limited from the field experiments or modeling due to various challenges for satellite remote sensing, i.e., constant cloud coverage, atmospheric correction, and difficulties to differentiate the Amazon River plume from normal Atlantic Ocean water. In this study, observations of ocean properties from the Visible Infrared Imaging Radiometer Suite (VIIRS) and sea surface salinity (SSS) from the Soil Moisture Active Passive (SMAP) missions in 2021 are used as an example to demonstrate the capability to monitor the Amazon River plume on daily basis and characterize the change of the ocean environment driven by the offshoots and migration of the plume in the western tropical Atlantic Ocean. The start, development, evolvement, dispersion, and dissipation of the Amazon River plume in 2021 in the western tropical Atlantic Ocean were effectively monitored. The extensive influence of the Amazon River runoff mostly occurred between June and November. The water diffuse attenuation coefficient at 490 nm (K d (490)) and SSS were significantly impacted by the Amazon River plume in the vast region of the western tropical Atlantic Ocean. The routes of the Amazon River plume that flowed into the Atlantic Ocean were highly variable. High turbidity, low salinity plume water with K d (490) reaching ~1.0 m−1 and SSS ~25 practical salinity unit (psu) was found in a region over 500 km from the Amazon River Estuary. The comparison of the Amazon River plume in 2020 and 2022 further shows the complication of the driving forcing and inter-annual variability of the Amazon River plume. The combination of gap-free satellite ocean color and SSS observations provided a new tool to monitor the short-term variability of the Amazon River plume and assess its seasonal and interannual impact on the ocean environment in the western tropical Atlantic Ocean.

The evolutive role of shoot apical meristems in the adaptation of angiosperms to life at sea and the jump to potential environmental biotechnology applications

Seagrasses have adapted to a submerged lifestyle in seawater through a complex set of evolutionary processes. However, they show sensitivity to increases in natural salinity levels such as those commonly found in discharges of desalination plants, which have exponentially grown due to water scarcity in highly populated temperate areas, such as the Mediterranean basin. This study assessed the effects of brine-derived hypersalinity on the Mediterranean seagrass Posidonia oceanica, focusing on the metabolic responses of shoot apical meristems (SAMs). Although most physiological and genetic studies have used leaves, SAMs are more directly correlated with plant survival and might be more responsive to salinity stress. The experiments were: a controlled mesocosm of more than six practical salinity units (psu) over natural levels using either artificial salts or desalination brine and field transplantation experiments comparing two sites following the dilution plume of the brine (+5 and + 2 psu) with control. Hydrogen peroxide (H2O2), thiobarbituric acid reactive substances (TBARS), and ascorbate were measured to determine oxidative stress and damage, as well as relative expression of genes related to osmotic regulation and oxidative responses. Overall, relative expression of genes related to osmotic regulation (SOS1, SOS3, AKT 2/3) and oxidative stress (STRK1, CAT, MnSOD, FeSOD, APX, GR) was higher in SAMs at higher brine exposures, indicating a more active metabolic response in this organ. Similarly, reactive oxygen species (ROS) production and lipid peroxidation were higher closer to brine discharge and total ascorbate lower, indicating a correlative response with stressor intensity. These findings confirm that SAMs play an essential role in P. oceanica hypersalinity responses and its adaptation to life in the marine environment. Finally, the use of P. oceanica SAMs in this study is highly recommended for an early detection of threats caused by desalination brines that may cause physiological damage and meadow regression.

Influence of different stocking densities of pearlspot (Etroplus suratensis) on plankton diversity indices of biofilm-based rearing system

Aim: Biofilm-based aquaculture systems, proven cost-effective, reduce the need for expensive supplementary feed. The biofilm acts as a natural planktonic food source for reared organisms. Previous research highlighted the influence of fish stocking density and biomass on plankton diversity in different aquaculture settings, underscoring the significance of these factors in biofilm-based systems. Hence, the present study attempted to understand how varying stocking densities of pearlspot (Etroplus suratensis) influence plankton diversity indices in the biofilm-based aquaculture system. Methodology: The present experiment was designed in 5 Practical Salinity Unit (PSU) brackishwater with soil-bottomed FRP circular (500 L) tanks for 60 days. The sugarcane bagasse was used as a substrate forbiofilm production after fertilization with cow dung, urea, and lime. Fries of pearlspot (4.67 ± 0.04 mm 1.71 ± 0.03 g-1) were stocked at four different stocking densities in triplicates viz T1 (50 nos); T2 (100 nos), T3 (150 nos) and T4 (200 nos) fish fry m-3 in the biofilm-based rearing system. Plankton samples were collected from water and biofilm deposited on the substrate and analyzed for diversity indices. The Shannon Diversity Index, Simpson Diversity Index, Simpson Dominance Index, and Margalef Richness Index were used to compute the plankton diversity indices in different treatments using standard equations. Results: In the current investigation, lower stocking densities with lower fish biomass were associated with significantly higher (P≤0.05) Shannon Diversity Index, Simpson Diversity Index, and Margalef Richness Index in water. In contrast, the Simpson Dominance Index in water showed a significantly lower (P≤0.05) value for treatments with lower fish biomass than for treatments with higher biomass. Interpretation: The values obtained for various diversity indices indicated that a biofilm-rearing system with lower E. suratensis biomass produced more planktonic abundance, evenness, and species richness. Key words: Biofilm, Pearlspot, Plankton diversity, Stocking density

NITROGEN LIMITATION AND CHANGES IN SALINITY AFFECTS CHEMICAL-PROXIMATE-COMPOSITION OF Thalassiosira weissflogii

Microalgae are a crucial factor in aquaculture activities, since they are used as live food in the larvae stage of shrimp, mollusks, and fish. Nevertheless, just a few microalga species are produce. In this study we evaluate the effect of salinity at 25, 35, 45 and 55 practical salinity unit (psu), in F/2 media as control group and two nitrogen (NaNO3) limited media, F/4 and F/8 on the diatom Thalassiosira weissflogii. To value the development of the diatom, kinetic growth curves, pH, biomass production and chemical-proximate composition were estimated. The highest growth rate and organic matter values were in media F/4 and 35 psu. Meanwhile, media F/8 at 55 psu presented the highest amount of ash and a very low growth rate. T. weissflogii is affected by high salinity concentration, however the limitation of nitrogen with 25 and 35 psu did not affect growth considerably. Concerning the chemical-proximate composition of T. weissflogii, media F/4 at 25 psu, presented the highest percentages of carbohydrates and proteins. In the same way, medium F2 at 25 psu, reported the highest amounts of carotenes. The results showed that high salinity was the variable that major affected the cell density, biomass production and chemical-proximate composition of T. weissflogii.

Species-specific metabolome changes during salinity downshift in sub-Arctic populations of Mytilus edulis and M. trossulus

The blue mussels Mytilus edulis and Mytilus trossulus are ecologically and economically important species distributed widely across the Northern Hemisphere. Understanding their behavioral and physiological disparities is crucial for assessing their ecological success and aquacultural value. The recent finding of non-native M. trossulus in the White Sea raises concerns regarding its potential competition with native M. edulis and its prospective spread in light of climate change and surface water freshening. We investigated the responses of M. edulis and M. trossulus to salinity variations by examining shell closure thresholds and tissue levels of 35 metabolic intermediates in mussels acclimated to different salinities (25, 16, and 10). The salinity threshold for valve closure was similar in both studied species, but M. trossulus consistently opened at lower salinities (by 0.2–0.7 practical salinity units) compared to M. edulis. Salinity-induced changes in metabolite levels were similar between the two species. Taurine emerged as the dominant osmolyte, comprising over 50% of the total free amino acid pool, with aspartate and glycine contributing 15–30%. Concentrations of taurine, glycine, and total free amino acids declined with decreasing salinity. Taurine to glycine ratios were higher in M. edulis and increased in both species with declining salinity. Acclimation salinity significantly influenced urea cycle intermediates and methionine sulfoxide content, a cellular biomarker of amino acid oxidation. Species-specific differences were observed in purine metabolism, with higher levels of GMP and AMP found in M. edulis. Likewise, aromatic amino acids and histidine levels were higher in M. edulis compared to M. trossulus. However, no evidence suggests superior adaptation of M. trossulus metabolism to hypoosmotic stress compared to M. edulis. Further research is necessary to elucidate the functional implications of subtle metabolic differences between these Mytilus congeners and their ecological consequences in changing marine environments.

Surface snow bromide and nitrate at Eureka, Canada, in early spring and implications for polar boundary layer chemistry

Abstract. This study explores the role of snowpack in polar boundary layer chemistry, especially as a direct source of reactive bromine (BrOx = BrO + Br) and nitrogen (NOx = NO + NO2) in the Arctic springtime. Surface snow samples were collected daily from a Canadian high Arctic location at Eureka, Nunavut (80° N, 86° W) from the end of February to the end of March in 2018 and 2019. The snow was sampled at several sites representing distinct environments: sea ice, inland close to sea level, and a hilltop ∼ 600 m above sea level (a.s.l.). At the inland sites, surface snow salinity has a double-peak distribution with the first and lowest peak at 0.001–0.002 practical salinity unit (psu), which corresponds to the precipitation effect, and the second peak at 0.01–0.04 psu, which is likely related to the salt accumulation effect (due to loss of water vapour by sublimation). Snow salinity on sea ice has a triple-peak distribution; its first and second peaks overlap with the inland peaks, and the third peak at 0.2–0.4 psu is likely due to the sea water effect (a result of upward migration of brine). At all sites, snow sodium and chloride concentrations increase by almost 10-fold from the top 0.2 to ∼ 1.5 cm. Surface snow bromide at sea level is significantly enriched, indicating a net sink of atmospheric bromine. Moreover, surface snow bromide at sea level has an increasing trend over the measurement period, with mean slopes of 0.024 µM d−1 in the 0–0.2 cm layer and 0.016 µM d−1 in the 0.2–0.5 cm layer. Surface snow nitrate at sea level also shows a significant increasing trend, with mean slopes of 0.27, 0.20, and 0.07 µM d−1 in the top 0.2, 0.2–0.5, and 0.5–1.5 cm layers, respectively. Using these trends, an integrated net deposition flux of bromide of (1.01 ± 0.48) × 107 molec.cm-2s-1 and an integrated net deposition flux of nitrate of (2.6 ± 0.37) × 108 molec.cm-2s-1 were derived. In addition, the surface snow nitrate and bromide at inland sites were found to be significantly correlated (R = 0.48–0.76) with the [NO3-]/[Br-] ratio of 4–7 indicating a possible acceleration effect of reactive bromine in atmospheric NOx-to-nitrate conversion. This is the first time such an effect has been seen in snow chemistry data obtained with a sampling frequency as short as 1 d. BrO partial column (0–4 km) data measured by MAX-DOAS show a decreasing trend in early spring, which generally agrees with the derived surface snow bromide deposition flux indicating that bromine in Eureka atmosphere and surface snow did not reach a photochemical equilibrium state. Through mass balance analysis, we conclude that the average release flux of reactive bromine from snow over the campaign period must be smaller than the derived bromide deposition flux of ∼ 1 × 107 molec.cm-2s-1. Note that the net mean fluxes observed do not completely rule out larger bidirectional fluxes over shorter timescales.

Colonisation patterns and reef growth of the invasive serpulid Ficopomatus enigmaticus in a Greek coastal lagoon

The reef-building serpulid polychaete Ficopomatus enigmaticus is an invasive species in brackish waters in tropical and temperate zones worldwide and often has massive impacts on ecosystems and anthropogenic activities. In Prokopos Lagoon in Greece, the species has constructed large reefs over the past few decades which occupy a large area of the lagoon. In this study, we used high-resolution aerial photography to map the distribution of these reefs, and conducted a one-year field survey to investigate settlement and growth parameters, including abundance, biomass and body length, using artificial settlement panels. We correlated our findings with physicochemical measurements, specifically focusing on temperature, salinity and dissolved oxygen. Salinity exhibited marked and rapid fluctuations, ranging from less than 2 to over 42 Practical Salinity Units (PSU). These variations did not affect the adult population, but settlement only occurred at salinities above approximately 10 PSU and only when water temperatures surpassed 18–20 °C. Settlement was reduced when temperatures were high (> 28 °C) but was not affected by high salinity. The maximum abundance measured was 880 000 individuals m−2, and reefs attained a weight of 18 kg m−2 in 11 months. This study contributes further to understanding the species’ environmental tolerances and life cycle, which in turn may help to design future management measures.

Protist communities of microbial mats from the extreme environments of five saline Andean lagoons at high altitudes in the Atacama Desert.

Heterotrophic protists colonizing microbial mats have received little attention over the last few years, despite their importance in microbial food webs. A significant challenge originates from the fact that many protists remain uncultivable and their functions remain poorly understood. Metabarcoding studies of protists in microbial mats across high-altitude lagoons of different salinities (4.3-34 practical salinity units) were carried out to provide insights into their vertical stratification at the millimeter scale. DNA and cDNA were analyzed for selected stations. Sequence variants classified as the amoeboid rhizarian Rhogostoma and the ciliate Euplotes were found to be common members of the heterotrophic protist communities. They were accompanied by diatoms and kinetoplastids. Correlation analyses point to the salinity of the water column as a main driver influencing the structure of the protist communities at the five studied microbial mats. The active part of the protist communities was detected to be higher at lower salinities (<20 practical salinity units). We found a restricted overlap of the protist community between the different microbial mats indicating the uniqueness of these different aquatic habitats. On the other hand, the dominating genotypes present in metabarcoding were similar and could be isolated and sequenced in comparative studies (Rhogostoma, Euplotes, Neobodo). Our results provide a snapshot of the unculturable protist diversity thriving the benthic zone of five athalossohaline lagoons across the Andean plateau.

Influence of Salinity on the Partitioning Behavior of Six Commonly Used Pesticides in Fish Eggs.

Salinity has been reported to impact the octanol-water partition coefficient of organic contaminants entering aquatic ecosystems. However, limited data are available on the impacts of salinity on their partitioning from the aqueous phase to adjacent organic compartments. The pesticides bifenthrin, chlorpyrifos, dicloran, myclobutanil, penconazole, and triadimefon were used to investigate the effects of salinity on their partitioning to capelin (Mallotus villosus) eggs in 5 practical salinity units (PSU) versus 25 PSU artificial seawater (ASW). The partitioning coefficient was significantly higher in 25 versus 5 PSU ASW for bifenthrin, chlorpyrifos, dicloran, penconazole, and triadimefon by 31%, 28%, 35%, 28%, and 20%, respectively, while for myclobutanil there was no significant difference. Moreover, pesticide partitioning to store-bought capelin eggs was consistent with the partitioning observed for the standard assay species, inland silversides (Menidia beryllina) eggs, after partitioning between the eggs and exposure solution had reached a state of equilibrium. The present study illustrates the importance of considering the influence of salinity on the environmental partitioning and fate of hydrophobic organic contaminants in aquatic ecosystems. Environ Toxicol Chem 2024;43:299-306. © 2023 SETAC.

Myo-inositol improves osmoregulation by promoting lipid utilization in Nile tilapia (Oreochromis niloticus)

This research explores the influence of myo-inositol (MI) on the hypertonic stress adaptation of Nile tilapia (Oreochromis niloticus) by increasing lipid utilization under long-term salinity stress. A two-factor interactive experiment was used, and diets containing either normal lipids (NL, 7%) or high lipids (HL, 15%) contained three MI concentrations of 0, 400, and 1200 mg/kg in each lipid diet. Fish were fed six diets under 20 psu (practical salinity units [psu]) for eight weeks. Under long-term hypertonic stress, dietary MI supplementation significantly improved the growth performance of O. niloticus (P < 0.05). Dietary 1200 mg/kg MI supplementation significantly promoted protein deposition and reduced fat accumulation in the whole fish (P < 0.05). The HL diet significantly caused lipid accumulation in the liver, while dietary 1200 mg/kg MI addition alleviated lipid accumulation by promoting the expression of genes related to lipolysis (PPAR-α; ATGL and CPT) and inhibiting the expression of genes related to lipid synthesis (SREBP; DGAT and FAS) (P < 0.05). In addition, 400 mg/kg MI significantly reduced serum lipids (TG and LDL-C) (P < 0.05). qPCR showed that the purity of dietary 1200 mg/kg MI significantly upregulated the expression of an ion transporter-related gene (NKA) in gills (P < 0.05). Meanwhile, 400 mg/kg and 1200 mg/kg MI significantly affected the content of fatty acids in the liver and gill (P < 0.05). In addition, dietary 1200 mg/kg MI supplementation significantly increased the antioxidant capacity in the liver of O. niloticus. In conclusion, dietary supplementation with 1200 mg/kg MI could regulate lipid metabolism, promote lipid utilization and improve the osmotic regulation ability of O. niloticus.

Comparative Transcriptome and WGCNA Analysis Reveal Molecular Responses to Salinity Change in Larvae of the Iwagaki Oyster Crassostrea Nippona.

The Iwagaki oyster Crassostrea nippona is an important aquaculture species with significant potential for large-scale oyster farming. It is susceptible to the fluctuated salinity in the coastal area. In this study, we compared the transcriptome of Crassostrea nippona larvae under variant conditions with low-salinity stress (28, 20, 15, 10, and 5 practical salinity units (psu)) for 24h. KEGG enrichment analysis of differentially expressed genes (DEGs) from pairwise comparisons identified several free amino acid metabolism pathway (taurine and hypotaurine, arginine and proline, glycine, and beta-alanine) contributing to the salinity change adaptation and activated "lysosome" and "apoptosis" pathway in response to the low-salinity stress (10 and 5 psu). Trend analysis revealed sustained upregulation of transmembrane transport-related genes (such as SLC family) and downregulation of ribosomal protein synthesis genes faced with decreasing salinities. In addition, 9 biomarkers in response to low-salinity stress were identified through weighted gene co-expression network analysis (WGCNA) and validated by qRT-PCR. Our transcriptome analysis provides a comprehensive view of the molecular mechanisms and regulatory networks underlying the adaptive responses of oyster larvae to hypo-salinity conditions. These findings contribute to our understanding of the complex biological processes involved in oyster resilience and adaptation to changing environmental conditions.

Size effect of graphene oxide from quantum dot to nanoflake on the mobility of nanoplastics in seawater-saturated sand

Marine sedimentary environment serves as an important sink of terrigenous nanoplastics (NP) and graphene oxides (GO). In this study, we discovered that GO of varying sizes exhibited distinct binding modes with 200 nm NP in 35 practical salinity unit (PSU) seawater, resulting in varying impacts on the mobility of NP in porous media. GO-8, with a size of 8±2 nm, firmly adhered to the surface of NP and formed stable primary heterogeneous aggregates, which promoted NP mobility and increased the mass recovery of effluent (Meff) from 24.74% to 31.08%. GO-250 (246±10 nm) partly enveloped NP and only slightly increased the volume of heteroaggregates, which had minimal effect on NP transport. Conversely, GO-850 (855±55 nm) wrapped numerous NP particles to form large secondary heteroaggregates that clung to sand surfaces, providing additional attachment sites for NP, resulting in complete inhibition of NP mobility in porous media (Meff = 0%). In brackish water with 3.5 PSU, all GO-8, GO-250 and GO-850 achieved enhanced mobility of NP, with Meff increasing from 50.35% to 85.62%, 69.45% and 75.41%, respectively. The results indicate that GO size effects on NP mobility are also salinity-dependent.

Development of a microplate-based novel toxicity bioassay using Chlorophyta and Phaeophyceae macroalgae.

Macroalgae are one of the main producers in marine environments. However, only a few toxicity test methods have been established that use reference strains of macroalgae to evaluate the effects of chemicals on the growth and reproduction of macroalgae to monitor water quality. We selected reference strains of Chlorophyta, Ulva aragoënsis; Phaeophyceae, Ectocarpus siliculosus; and wakame, Undaria pinnatifida, as test species to establish a microplate-based method to investigate the toxicity of potassium dichromate, 3,5-dichlorophenol, and two common herbicides (diuron and simazine). We determined the growth of the three macroalgae in their early life stages and during the sporangia formation stage in E. siliculosus under laboratory conditions. We observed that the growth and sporangia formation in these algae were impaired in a dose-dependent manner. Additionally, we investigated the sensitivity of these macroalgae by comparing the toxicity values of toxicants used in this study with those obtained from a database. Compared to other microalgae and plant species, macroalgae showed a relatively high sensitivity to organic compounds, including herbicides. Growth tests using U. aragoënsis and E. siliculosus produced reliable results at 0-32 and 25-32 practical salinity units (PSU), respectively. The tests established in this study could test the toxicity of chemical substances in macroalgae and are thus expected to contribute to a better understanding of the environmental risks of chemical substances on aquatic biota. The tests could be applied to all effluent toxicity tests used for the management of seawater and brackish water quality.

Experimental assessment the effects of saline discharge on plankton community of the recipient freshwater ecosystem – Inputs to scientific ballast water management of inland ports

Expanding international trade has accelerated the transfer of ballast water worldwide, the change in salinity is expected to be detrimental to recipient areas’ species, especially to freshwater ports’. This study simulated the changes in freshwater plankton community with respect to salinity stress resulting from ballast water discharged. The prepared saline water (simulates ballast water that meets D-2 standards) was discharged into the collected freshwater samples (from natural lake) at different volume ratios, which represented the salinity changes in localized areas of the receiving waters. The samples were counted and identified for ≥ 10–50 µm and ≥ 50 µm organisms with FDA (Florescein Diacetate) & CMFDA (Chloromethylflurescein Diacetate) method and Microscope counting method. From the first day after saline water discharged, it was found that when water salinity reached 12.2 PSU (Practical salinity units), the density of ≥ 50 µm organism was about 0 ind./m3, while only when water salinity reached 24.8 PSU, the density of ≥ 10–50 µm organisms was 0 cells/mL from the sixth day after saline water discharged. When the salinity was less than or equal to 2.1 PSU, the composition of species of ≥ 50 µm organisms in samples were similar to the control group, but for ≥ 10–50 µm organisms, when the salinity was less than or equal to 6.5 PSU, the community structure in samples were similar to the control group. The ≥ 10–50 µm and ≥ 50 µm organisms, these two groups were differently responsive to the changes in salinity. Besides, when the change of water salinity doesn’t exceed 6.5 PSU, the density of plankton first decreased and then increased, but when salinity changes exceed 6.5 PSU, the density of plankton exhibited a great decline during ten days. The results indicated that high-salt ballast water discharge could negatively affect freshwater harbor organisms, the salinity indicators could provide the basis for future studies of actual water samples, pay attention to the impact of ballast water discharge on inland ports is urgent.

The history of a cluster of large icebergs on leaving the Weddell Sea pack ice and their impact on the ocean

AbstractThe life history and oceanic impact of three very large icebergs that escaped together from the Weddell Sea sea ice, near the tip of the Antarctic Peninsula, are traced from March 2014. Despite the initial proximity of these three icebergs, they followed very different trajectories across the South Atlantic until their eventual break-up and melting 1 year later. The largest, giant iceberg, B17a, spent extensive periods grounded near two different islands. The triplet's gradual melting is examined through the impact on the icebergs' dimensions, but also the meltwater's oceanic influence on the local salinity and primary productivity. It is found that there was generally a significant local surface and mixed-layer freshening of a few tenths of a practical salinity unit, up to several hundred kilometres away from the 10–20 km-sized icebergs. In contrast, the chlorophyll impact was highly temporally variable, although it tended to be larger in the summer. Break-up of these large icebergs did not occur until near the end of their life. We also show that modelling the trajectories of individual very large icebergs can be reasonable for up to 2 weeks if the characteristics of the iceberg and the local ocean and atmospheric forcing are well known.

A forecast effects of climate change and anthropogenic compounds in Gambusia holbrooki: ecotoxicological effects of salinity and metformin

Due to global warming and extreme weather events, estuarine and coastal ecosystems are facing sudden fluctuations in salinity. These ecosystems are also threatened by organic and inorganic compounds that increase water pollution. Metformin is an antidiabetic drug commonly used by patients with type-2 diabetes, and an increase in environmental concentration has been recorded. To better understand the impacts of these two stressors on aquatic organisms, this study assessed: 1) the acute (96 h) ecotoxicological effects (antioxidant and biotransformation capacity, oxidative damage, energetic reserves, and protein content, neurotoxicity) induced by a range of metformin concentrations in Gambusia holbrooki under different salinities (17, 24, 31 expressed as Practical Salinity Units - PSU); and 2) the same endpoints after chronic exposure (28 d) under a range of metformin concentrations at a salinity of 17. The results obtained from the acute exposure showed interactions between salinity and metformin in G. holbrooki superoxide dismutase (SOD) activity, body protein, and glycogen (GLY) contents. The results revealed that an increase in salinity can modulate the response of G. holbrooki to metformin. Chronically exposed organisms showed that metformin led to a significant decrease in SOD activity at most of the tested concentrations (0.5, 1.0, and 10 µg/L). In addition, glutathione S-transferases increased and glutathione peroxidase activity decreased significantly at concentrations of metformin of 5 and 10 at the µg/L, respectively. Therefore, overall, metformin can lead to potential oxidative stress in G. holbrooki the highest metformin concentrations tested and the GLY content in G. holbrooki increased after exposure to metformin concentrations of 0.5, 1.0 and 5.0 μg/L. Published studies have already shown that metformin alone can lead to oxidative damage in aquatic species, endangering the biodiversity of aquatic ecosystems. Therefore, additional ecotoxicological studies should be performed to characterize if other metformin concentrations combined with salinity, or other climate change-related factors, might impact non-target species. Standard toxicity bioassays may not be predictive of actual pollutants (e.g. metformin) toxicity under variable environmental conditions, and the investigation of a wider range of exposure conditions could improve the accuracy of chemical risk assessments.

A newly isolated alkaliphilic cyanobacterium for biomass production with direct air CO2 capture

Large-scale microalgal biomass production usually relies on concentrated CO2 as the carbon source which is a major logistical and economic challenge because of the required infrastructure and geospatial constraints of siting large algal production facilities close to concentrated CO2 sources. By taking advantage of enhanced CO2 mass transfer within alkaline media due to the reaction between CO2 and OH-, carbon can be directly captured from the ambient air and used by the algae. Achieving such goal requires algal strains that can grow fast under alkaline conditions. In this study, a newly isolated alkaliphilic cyanobacterial strain Cyanobacterium sp. PNNL-SSL1 was characterized for its temperature, pH, and salinity tolerance. The result shows that PNNL-SSL1 could sustain grow at pH above 11.2 but exhibited the highest growth rates at pH below 10.5. The strain is a warm season strain and grows the best at 10 PSU (practical salinity unit). The strain was then evaluated in indoor climate simulation ponds under outdoor-relevant pond cultivation conditions with carbon supplied only through direct air CO2 capture at the culture surface. Two batch culture runs, at slightly different initial pH, exhibited average biomass productivity of 15.2 g m-2 day-1 on ash-free dry weight basis. CO2 was directly captured from the air at an average rate of 21.6 g CO2 m-2 day-1 (78.8 tons ha-1 yr-1), contributing 74% of the carbon fixed in the biomass. The results demonstrated that PNNL-SSL1 is a promising strain for biomass production using air CO2 as the carbon source.

Behavioural and physiological responses to salinization and air exposure during the ontogeny of a freshwater South American snail.

Salinization is of global concern, threatening freshwater biodiversity. Salinity tolerance is highly variable and therefore needs to be evaluated on a species-specific basis. An estuarine population of Chilina dombeiana, a freshwater gastropod endemic to Chile and classified as vulnerable, has been recently found in the Biobío River's mouth, suggesting some degree of tolerance to brackish waters. This study evaluated the survival, behaviour (medium preference) and physiology of C. dombeiana when exposed to salinities higher than freshwater, thus elucidating the potential mechanisms used to survive salinization. Chilina dombeiana belongs to the Pulmonate group;, so we evaluated oxygen uptake in air and water, aiming to evaluate emersion as a potential avoidance response to a progressive salinity increase. Complete embryo development was observed for salinities ≤ 16 PSU (practical salinity units) but hatching rates above 50% were only achieved in freshwater (0 PSU). It was also found that salinity had stage-specific effects during embryonic development. In adults, acute exposure to brackish water (12 PSU) caused a decrease in oxygen consumption (compared to freshwater), in the ammonium excretion rates and in the percentage of muscular water content. Although C. dombeiana was able to take up oxygen in both mediums, survival in air decreased over time (days), which correlates with the behavioural preference to remain submerged, even at elevated salinities. Considering the survival of adults and embryos decreased as salinity increased and the lack of an avoidance behaviour or a physiological ability to maintain homeostasis at salinities higher than freshwater, our results suggest this snail could be adversely affected by salinization in the long term. Furthermore, given the ability of C. dombeiana to uptake oxygen in both mediums, it should be considered as a facultative air breather snail, rather than a strictly aquatic species.

Ocean Salinity Retrieval and Prediction for Soil Moisture Active Passive Satellite Using Data-to-Data Translation

Sea surface salinity (SSS) is a key parameter in physical oceanography, global hydrological, biochemical cycles, and climate change studies. L-band radiometers onboard satellites, such as Soil Moisture Active Passive (SMAP), Soil Moisture and Ocean Salinity (SMOS), have played a crucial role in monitoring and analyzing the global SSS in the past decades. This study presents a method to retrieve and predict the SMAP SSS through data-to-data translation (D2D) based on conditional generative adversarial networks. The model was constructed from the polarized brightness temperatures, differences in the polarized brightness temperatures from the L band of the SMAP satellite, and sea surface temperature and sea surface wind speed from the European Center for Medium-Range Weather Forecasts data from April 2015 to July 2020, and applied to produce SMAP SSS. A comparison between the SMAP salinity and the D2D-generated SMAP salinity showed excellent agreement, evaluated through bias = 0.016, root mean square error (RMSE) = 0.173 in practical salinity unit (psu) units, and correlation coefficient (CC) = 0.985. Furthermore, a comparison between the D2D-generated and buoy-observed salinities showed good agreement (bias = -0.031 psu and RMSE = 0.196 psu, CC = 0.971). Additionally, the results of the one-month prediction model were also in good agreement with the SMAP SSS (bias = 0.028 psu and RMSE = 0.218 psu, CC = 0.977). Consequently, the D2D-based model can be effectively used to generate SMAP SSS information and can be applied to various microwave satellites.