The rapid development of highway transportation has led to a gradual deterioration of the sound environment along highways. The theory of periodic structures provides a new approach for the design of traffic noise barriers. However, the lack of practical numerical models and on-site experimental validation has hindered the widespread application of periodic structure sound barriers. This study investigates the noise reduction performance of nested structure periodic sound barriers. Results from semi-anechoic chamber tests demonstrate excellent wave attenuation at the air gap of the barrier, achieving a peak noise reduction of 16 dB without the addition of any sound-absorbing materials or treatment measures, particularly at local peaks in the highway noise spectrum. To enhance the noise reduction performance of nested structure periodic sound barriers, three optimization measures were explored: filling sound-absorbing materials inside the scattering elements, adding micro-perforated panels, and their combined effects. The results indicate that both porous sound-absorbing materials and micro-perforated panels can improve noise reduction effectiveness, especially outside the air gap. In the optimization design of nested structure periodic sound barriers, adding porous sound-absorbing materials inside the micro-perforated panels effectively enhances noise reduction within the air gap. Considering the abundance of abandoned w-beam guardrails in highway projects, which also meet the requirements of scattering elements, this study proposes the use of abandoned w-beam guardrails as scattering elements. This approach aligns with principles of cost-effectiveness and environmental sustainability, and it provides design guidance to promote the application of nested structure periodic sound barriers in traffic noise control.