Ppm IAA Research Articles

Potato (Solanum tuberosum L.) Planlet Acclimatization on Various Auxin Concentrations and Growing Media

The provision of quality potato seeds is a problem faced by farmers. Tissue culture propagation is an effort to increase the availability of quality seeds. The success of tissue culture is influenced by the ability to control environmental conditions, growth regulators, and growing media at the acclimatization stage. The research aims to determine the interaction between the use of auxin concentrations and the planting medium and to obtain the appropriate concentration of auxin and planting medium for potato acclimatization. The research used a split-plot design with 2 factors and 3 replications. The main plot is the auxin concentration consisting of 3 levels; 1 ppm, 2 ppm, and 3 ppm. Subplots are planting media consisting of 3 levels; husk charcoal:vermicompost, cocopeat:vermicompost, and husk charcoal:cocopeat:vermicompost. Data were analyzed using ANOVA and followed by DMRT at a 5% level. The results showed that there was an interaction between the treatment of 3 ppm IAA concentration and the planting medium of husk charcoal:cocopeat:vermicompost on the plant height parameter 4 WAP. The IAA concentration of 3 ppm was better than the concentration of 1 ppm in the parameter number of leaves 8 WAP. The growing media of husk charcoal:cocopeat:vermicompost was better than the growing medium of charcoal husk:vermicompost on the parameters of plant height 8 WAP, number of leaves 8 WAP, leaf length and leaf width 8 WAP.

Pengaruh waktu induksi dan ZPT terhadap pertumbuhan kalus kotiledon kacang panjang (Vigna unguiculata subsp. sesquipedalis)

Yard-Long bean plants are an agricultural commodity that has great potential to be developed because they are easy to cultivate, their market share is also quite high but there are still problems in meeting the need for sufficient seeds and they are still susceptible to pests and disease. Therefore, efforts need to be made to increase production and induce variations. One method that can be used is tissue culture for the induction of somaclonal variations and/or the formation of transgenic plants. This study aims to determine the effect of variations in callus initiation time and the effect of Plant Growth Regulators (PGR) administration on the growth of long bean cotyledon explant callus. Seed sterilization was carried out using detergent, 10% sodium hypochlorite, 70% alcohol and sterile distilled water. The results of the research showed that variations in callus initiation time on MS media with the addition of 2,4-D PGR and the use of several variations of PGR in subcultures were able to initiate callus and influence callus size, callus color, and the percentage of browning of Yard-long bean cotyledon explants. Callus initiation time of 4 weeks with a combination of 2 ppm BAP concentration with 0.1 ppm IAA produced the largest callus size and brownish white callus color. The addition of PGR during subculture had a smaller browning percentage than the treatment without PGR.

Pengaruh Kombinasi ZPT IAA dan BAP terhadap Pertumbuhan Eksplan Vanili (Vanilla planifolia Andrews) secara In Vitro

Vanilla (Vanilla planifolia Andrews) is one of the commodity values of plantation crops in Indonesia which has a high economy. Vanilla cultivation is done to get vanilla fruit which is the raw material for making vanilla essence. Vanilla can be cultivated generatively and vegetatively. However, in the field, generative cultivation of vanilla through seeds is considered very detrimental. Because it takes a long time and a low success rate. Meanwhile, vegetative cultivation of vanilla through cuttings is also considered less effective. Because, often seedlings from tendril cuttings are susceptible to stem rot disease and their growth is not uneven. So that planting on a large scale will be difficult to do. Therefore, to obtain more vanilla seeds, it is necessary to use certain technologies such as in vitro. The purpose of this research was to determine the effect of the combination ZPT IAA and BAP on the growth of vanilla explants. The research used was RAL non-factorial with 5 treatments, namely P1 (0 ppm IAA + 2 ppm BAP), P2 (0,1 ppm IAA + 1,5 ppm BAP), P3 (0,3 ppm IAA + 1 ppm BAP), P4 (0,5 ppm IAA + 0,5 ppm BAP), and P5 (0,7 ppm IAA + 0 ppm BAP). Each treatment was repeated 5 times. The basic media used is MS. The results showed that the P3 and P5 treatments affected the ability to explants germinate, explants did not stagnate, and explants form shoots. However, all treatments did not affect the ability of explants to produce roots.

Efficient Propagation Methods and Management Implication of Aconitum chasmanthum Stapf ex Holmes: A Critically Endangered and Endemic Medicinal Herb of Kashmir Himalaya

Background: Knowledge about propagation of rare and endangered plant species is very important for their conservation and sustainable utilization. Therefore, it is imperative to understand different reproductive aspects of such species for their conservation and management. It is in this backdrop different propagation method of Aconitum chasmanthum- a Critically Endangered and Endemic Medicinal plant species has been undertaken. Methods: Seed germination was carried out using different media. For each treatment mean germination time (MGT) was also calculated. Different agro-techniques were also developed in different soil textural classes. The fresh underground tubers of the species were collected from natural population and were split longitudinally into 2, 3 and 4 pieces depending upon the size of the parental tuber containing a portion of shoot apex. The split cuttings were treated with 100 ppm IAA, IBA and GA3 for 48 hours and were sown in sandy loam soils under pots at Herbal Garden. The pots were kept in diffused light to monitor the leafy shoot generation, survival and comparison with the control (untreated cuttings). Result: The present study revealed that seeds of the species remain dormant for a pretty long period and do not germinate in darker conditions. Among various treatments the species showed 90.0±8.66% germination when chilled for 20 days with mean germination time (MGT) of 13.678±1.874 days as against 10.66±1.154% germination of the control. Although the plants grew nicely on various soils, maximum plant survival and vigorous growth was obtained in loamy textured soils. The plants responded to various nutrient treatments and in loamy soil the maximum dry biomass was shown by the plants treated with nitrogen (300 mg/kg soil) and NPK (500 mg/kg soil). The plants treated with NPK (500 mg/kg soil) produced 14.46±2.50 gm dry biomass as against the control of 5.23±0.750 gm per individual. The experimental manipulations also reveal that the species has a potential to propagate through tuber cuttings in sandy loam soils. The cuttings treated with IAA, IBA and GA3 showed 68.75% to 75.0% survival rate as against the 62.5% in control.

The effect of murashige and skoog (MS) modified medium and several types of auxins on the growth of stevia (Stevia rebaudiana bertoni) in vitro

The aims of this research is to testing the use of several types of auxins with the same concentration (0.2 ppm IAA; 0.2 ppm NAA; 0.2 ppm IBA) and modification of MS media (1/4 MS; 1 / 2MS; MS full ). Parameters observed included roots number, roots length, plantlets length and velocity of root emergence. The result shown that roots appeared influenced by the modification of MS media, the fastest root appearance was in the treatment using full MS and 1/2 MS on 14 and 15 DAS (day after showing). The several types of auxin and the modification of MS media were very significant. The number of roots parameter also resulted from the interaction between the auxin type and the modification of MS media which had a very significant effect. At the end of the observation (30 DAS) highest number of roots with an average of 4.23 was found in the using NAA treatment, with 1/2 MS. The modification of MS media is more optimal increasing roots length is at 1/2 MS. The modification of MS media which is more optimal in increasing length of plantlets at full media and was not significantly different with 1/2 MS.

Studies on sexual compatibility and or incompatibility among Psidium species and their hybridity confirmation through ssr markers

Abstract The present investigation was carried out with an aim to determine the cross compatibility between Psidium species (P. chinensis, P. guineense, P. cattleianum and P. molle) and commercial guava cultivars (Arka Kiran, Arka Rashmi, Arka Mridula and Allahabad Safed). The results revealed that, P. chinensis and P. guineense were found to be good cross compatible male parents whereas, Allahabad Safeda and Arka Kiran were found to be good cross compatible female parents. However, no fruit set and seed set were obtained when P. cattleianum and P. molle were used as male parent indicating the cross incompatibility of these species with commercial guava cultivars which is reconfirmed using florescence microscopy technique to understand the pollen-pistil interaction between incompatible P. cattleianum and P. molle species with P. guajava cv. Allahabad Safeda. Result indicated that low fluorescence intensity along with poor pollen germination and improper pollen-tube growth could be the reason for incompatibility. In order to break this incompatibility, a modified pollination technique called stigma maceration technique was followed wherein stigma of the male parent macerated with different chemicals viz., carbohydrate source (2% sucrose), growth regulators (50 ppm GA3 and iaa) and micronutrients (50 ppm of each Boric acid, MgSO4, CaNO3 and KNO3) and pollinated twice i.e., on the day of flower opening and the same time of next day resulted in cross compatibility of P. cattleianum and P. molle with commercial guava cultivars. Further hybridity confirmation of interspecific hybrids using ssr markers revealed that, 8 markers were found to be co-dominant and confirmed the hybridity of interspecific hybrid combinations.

The Effect of IAA Concentration and Growing Media Composition on Banana Raja Bulu (Musa paradisiaca L.) Planlet Growth at Aclimatization Stage

Banana plants are an annua horticultural crops that has production capability without depend on the season and are widely cultivated in the tropic or subtropic areas. Tissue culture can produce good quality banana seedling in large quantities. Banana planlets from tissue culture require appropriate growing media and growth regulators such as auxin to stimulate their metabolic systems. The research aim to examine effect of IAA auxin hormone concentration, composition sand: compost: husk charcoal and its interactions on the growth of raja bulu banana plantlets. The research was conducted in July - September 2019 at Plant Physiology and Breeding Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang. The study used a complete randomized factorial design 4x2 with 5 replications. The first factor was IAA concentration (K) consists of 4 levels, K0: 0 ppm, K1: 30 ppm, K2: 60 ppm, K3: 90 ppm. The second factor was composition of growing media (M), M0: sand: compost: husk charcoal (1: 1: 1) and M1: sand: compost: husk charcoal (1: 1: 2). Data were analyzed by F test and proceeded by DMRT test at the level of 5%. The results showed that IAA significantly affected stem diameter, number of leaves, chlorophyll content and nitrate reductase activity of banana seedlings. The treatment of growing media significantly affected plant height and number of leaves. Concentration of 30 ppm IAA at all growing media increased significantly on stem diameter, number of leaves, chlorophyll content and nitrate reductase activity.

Effect of Azospirillum zeae and Seed Priming with Zinc, Manganese and Auxin on Growth and Yield Parameters of Wheat, under Dryland Farming

In this study, field experiments with wheat (Triticum aestivum L.) were conducted consecutively for 2 years. The main objective was to determine (1) effect of seed priming with auxin (IAA), zinc (Zn) or manganese (Mn) on growth and yield parameters of wheat under dry land farming; (2) influence of on-farm priming with Azospirillum under field conditions. In this study, the response of dryland wheat yield to soaking seeds in water, 2 ppm IAA, 0.2% Mn solution and 0.2% aqueous solution of Zn with or without Azospirillum zeae inoculation was investigated under field condition. Total grain yield was significantly enhanced for water primed seeds i.e., 1273 kg ha−1 (6.8%) or seeds primed with IAA, as compared to 1191 kg ha−1 in plants from non-primed seeds. Difference in grain yield between priming with water and 2 ppm IAA was not significant when compared with 1372 kg ha−1 (15%) for seeds primed with 0.2% Mn solution and 1440 kg ha−1 (20%) for seeds primed with 0.2% Zn solution. Grain yield was significantly increased for plants inoculated with Azospirillum, 1411 kg ha−1 (8%) as compared to 1191 t ha−1 for non-Azospirillum-inoculated seeds. Yield parameters, i.e., tiller no. per m−2, grain number per spike, and 1000-grain weight, were all significantly affected by priming treatments and Azospirillum inoculation. Grains with the high nitrogen and phosphorous content were recorded for the treatment where seeds were primed with Zn and inoculated with Azospirillum. In the present study, inoculation of wheat seeds followed by priming is a significant approach to enhance grain yield and grain nutrients (N and P) content.

Formation of lateral roots and root hairs of Ciherang rice as a result of Nostoc colonization

We performed an interaction study between the cyanobacteria Nostoc sp. CPG24 and GIA13a and the roots of Ciherang rice (Oryza sativa var. Ciherang) in a hydroponic system. For this purpose,14-day-old rice seedlings were cultivated in 10× BG11 N-free, aerated liquid medium. The plants were incubated at room temperature and grown in a 12:12 light–dark cycle. To better understand the role of Nostoc colonization on root growth, we exposed the rice samples to four different treatments: P1 (0.4 g Nostoc CPG24 strain inoculum), P2 (0.4 g Nostoc GIA13a strain inoculum), K1 (no inoculation of Nostoc and IAA), and K2 (addition of 15 ppm IAA). Regardless of the strain, we found that Nostoc filaments were attached to the root surface, particularly in the regions of formation and elongation of lateral roots and root hairs. The root elongation and number of lateral roots were high under the K2 and P2 treatments, whereas they were substantially reduced under the P1 treatment. We demonstrate that the Nostoc strain GIA13a induces the formation of new lateral roots, whereas the Nostoc strain CPG24 stimulates the formation of root hairs.

MICROPROPAGATION OF Labisia pumila USING EMBRYO CULTURE

Labisia pumila or commonly known as Kacip Fatimah is one of the plants that were used as a medicinal purpose. Previously, several studies on the regeneration of the plant through tissue culture had been conducted. L. pumila can be planted using macropropagation technique in which L. pumila can be propagated by using stem cuttings. However, the seed yields were low. This study was initiated to regenerate L. pumila using micropropagation from the embryo as the explant. Embryos were cultured on different strength MS culture mediums which are full–strength and half–strength whereby the half-strength MS medium was supplemented with 0.5 ppm benzylaminopurine (BAP) for shoot induction. Shoot formation was achieved from both media. High rate of shoot formation occurred on full-strength MS medium without plant growth regulators. Multiple shoots was established on half-strength MS medium containing 0.5 ppm BAP. Shoots elongation and plantlet establishment were produced with culture on half-strength MS medium with combination of 0.5 ppm BA and 0.5 ppm IAA.

Roots Induction and Growth of Shoot Cutting of Jirak (Eurya acuminata DC.) by Adding Some Growth Regulator Solutions in Various Planting Medium

The research about roots induction and growth of shoot cutting of Jirak (Eurya acuminata DC.) by adding some growth regulator solutions in various planting medium, was conducted from May until August 2017 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University. The aims of the research to find out effect of providing growth regulator solution with different types, used of planting medium and interaction of both factors the best to roots induction and growth Jirak (E. acuminata DC.) by cutting. The research used Complete Randomized Design (CRD) factorial with two factors and three replications. Factors A were growth regulator solution (a0: control, a1: IBA 100 ppm, a2: NAA 100 ppm, a3: IAA 100 ppm) and factors B were various planting medium (b0: garden soil, b1: sand, b2: charcoal husk). The research result were adding some growth regulator solutions and various planting medium significanly affected roots induction and growth of jirak and there is interaction on the high of plant. The treatment of 100 ppm IAA and treatment using sand medium obtain the highest result in increasing roots induction and growth of shoot cutting of Jirak.

Increasing the Growth and Quality of Red Chili with Growth Hormone from Endophytic Bacteria

<p>Red chili is a very important horticultural commodity in Indonesia having low productivity and quality. Cultivation method needs to be improved including the use of exogenous growth hormones. The purpose of this study was to determine (1) the optimum concentration of IAA and GA growing hormones from isolate B6.2 in stimulating plant growth and improving the quality of large red chili fruit; (2) molecular identity of the B6.2 bacterial isolate. The growth hormone content of B6.2 isolates using HPLC obtained 0.49 ppm IAA and 64.53 ppm GA. The growth hormone potential test on the growth and quality of chili was carried out with a concentration of 1, 3, 5 ml/l, while water and synthetic hormones was used as negative and positive control, respectively. The experimental design used was a Complete Random Design with the foliar spray application to the plant canopy three times during the growth period. The results showed the best concentration in increasing plant height, fruit weight, shooth wet, and dry weight compared to controls at the age of 76 days after planting (dap) was a concentration of 5 ml/l, with the values of 71.7±0.9 cm , 94.7±0.3 g, 11.5±0.43 g, and 1.4±0.09 g, respectively. The molecular identification showed that B6.2 isolate was classified as Bacillus vallismortis with 100% homology. The growth hormone from isolate B6.2 has the potential to increase growth and production of red chili plants.</p>

PENGARUH KONSENTRASI IAA DAN BERBAGAI JENIS MEDIA TUMBUH TERHADAP PERTUMBUHAN TANAMAN SELEDRI (Apium graveolens L.) DENGAN SISTEM BUDIDAYA HIDROPONIK FERTIGASI

The purpose of the study was to determine the effect of IAA concentration and various types of media growing on celery plant growth with a fertilizing hydroponic cultivation system in interaction as well as each of the main influences. The design used in this study is the Design of Divided Plots in the form of Complete Randomized Design consisting of two factors. The first factor is IAA (A) with a concentration of 0, 1, 10, 100 ppm while the second factor is Various Types of Growing Media (M), namely Cocopeat, Husk Charcoal, Sawdust Powder, Hydroton. The parameters observed were plant height (cm), economical wet weight (g), root volume (cm3), average net assimilation rate (g /cm2/day), average relative growth rate (g /day), number of tillers (fruit), root canopy ratio (g). The last observation data were analyzed statistically and continued with a BNJ follow-up test at the level of 5%. The results showed that the interaction of IAA concentrations and various types of media grew significantly on the parameters of the plant's wet weight, root volume, and root canopy ratio. The best treatment was found in a combination of treatment of 1.0 ppm IAA concentration and cocopeat growing media. The main effect of IAA concentration was real on all observed parameters. The best treatment is 1.0 ppm IAA concentration. The main influence of various types of media grows significantly for all observational parameters. The best treatment is the cocopeat media.

Production and Physiology Characteristics of Some Rubber Clones in Early Tapping with Growth Regulator

The study was aimed to study latex production and physiology character of some rubber clones in early tapping with the use of Plant Growth Regulators (PGR) and the relationship between production and latex yield characters. The research was conducted in KarangInong Farm, PTP-N I Kso PTP-N III, East Aceh. The research was prepared using two factors Nested Design, namely 5 clone factors and 7 hormone factors. The treatment was repeated twice, but nested in the clone factor, so the number of experimental units was 7 x 5 x 2 = 70. Each experimental unit consists of 4 plants with the total is 280 plants. The results showed that response of each clone and hormone was different for production parameters and latex yield character. Treatment of clone PB 340 and hormone H6 (600 ppm IAA + Kinetin 60 ppm) yield the highest production (g/p/s). The best Interaction of clone and hormone treatment is K4H4 (IRR 107 clone with IAA 500 ppm + Kinetin 60 ppm) as indicated by production parameters (38.04 g/p/s), sucrose content (9.14 mM, phosphoric acid (6.84 mM), Thiol (0.50 mM) and plugging index (16.34%)

Clonal propagation technique for mass multiplication of Noni (Morinda citrifolia) seedlings

A study was under taken to standardize the type of cutting, concentration of growth hormone and suitable rooting media to produce elite seedlings in Morinda citrifolia at Forest College and Research Institute, Mettupalayam. Investigation was carried out to standardize the treatments to induce root and shoot growth in different types of cuttings of Morinda citrifolia L. using two growth regulators (IAA, IBA) used in different concentrations (1000, 1500, 2000 and 2500 ppm) in two kinds growth medium (Sand and Coir) for different type of cuttings, 2500 ppm IBA treatment recorded its superiority in terms of maximum sprouting (85%), rooting (79%) and survival (70%) percentage, number of roots (15.83), root length (8.51 cm), number of shoots (4.00) and shoot length (9.43 cm) in leafy shoot cuttings, irrespective of rooting media used. From this current study it is concluded that, for macro clonal propagation of noni ( Morinda citrifolia L.), either leafy shoot cuttings or hard wood cuttings could be treated with 2500 ppm IBA or semi hard wood cuttings could be treated with 1500 ppm IAA and could be planted in coir pith medium for the production of elite planting materials.

Effects of Indole-3-Acetic Acid on the Growth Parameters of <i>Citrullus lanatus</i> (Thunberg) Matsum and Nakai

Effects of Indole-3-Acid (IAA) on Citrullus lanatus plant height, leaf number, branching and flower production were studied in order to evaluate the optimum concentrations required for growth and development. Foliar application using five different concentrations [1, 5, 10, 20 and 0 ppm IAA] was conducted twice at two weeks interval. The results suggest that as IAA concentration increases, plant height decreases with an optimum concentration of 5 ppm IAA. Mean plant height was also significantly different. More so, optimum concentration for leaf production and branching varied with varietal difference. Results obtained for number of branching suggest that 1 ppm IAA is optimum for variety A and 10 ppm is optimum for varieties B, C and D. Although flower production was poor during the study, high flower production is recorded for plants in 20 ppm IAA at 6 weeks after planting for all the varieties. This work establishes the inducing effects of different IAA concentration, which may be exploited for the commercial production of C. lanatus.Keywords: Watermelon (Citrullus lanatus), Indole-3-Acetic acid, Growth parameters, Leaf production, Plant Physiology.

Efficacy of IAA concentration and cutting length on rooting of stem cuttings in Spondias pinnata Linn.

An experiment was laid out in factorial randomized block design with 12 treatments and 3 replications which included three levels (10, 15 and 20 cm) of cutting length and four levels of IAA treatment (0 control, 500, 1000 and 1500 ppm). Each cutting was treated with different concentrations of IAA for 15 sec. Investigation revealed that 15 cm length of cuttings treated with 1500 ppm IAA gave the best results in terms of earliest bud (13.27 DAP) and leaf (16.32 DAP) initiation, highest success (66.67%), shoot length (34.89 cm), root length (13.49 cm), collar diameter (20.50 mm), root number (9.53), root diameter (6.91 mm), fresh shoot weight (45.47g), fresh leaf weight (14.07 g), dry shoot weight (21.15 g), dry root weight (2.67 g) and total biomass (29.80 g/plant).

Regeneration of Plantlets Through PLB (Protocorm-Like Body) Formation in Phalaenopsis ‘Join Angle X Sogo Musadian’

Selection and regeneration of specific hybrids of Phalaenopsis typically takes a long time since this plant usually reproduces through seeds. This study was conducted to examine the best medium and explants for regeneration of Phalaenopsis ‘Join Angle x Sogo Musadian’. In vitro and in vivo roots and leaves were used as explants, which were cultured in half-strength Murashige and Skoog medium supplemented with various combinations of plant growth regulators (TDZ, 2,4-D, NAA, BAP and IAA). The results showed that the in vitro roots produced the highest number of PLBs (49.3 PLBs) when they were cultured in medium containing 0.5 ppm NAA, 5 ppm BAP, and 0.5 ppm IAA. The in vitro leaf explants also regenerated PLBs, however, only two PLBs developed, i.e. when they were cultured in medium containing 1 ppm TDZ and 2 ppm 2,4-D. PLB germination into plantlets was performed by culturing each PLB on MS medium without plant growth regulators. Based on the results of the present study, it can be concluded that in vitro roots and half-strength MS medium supplemented with 0.5 ppm NAA, 5 ppm BAP, and 0.5 ppm IAA are the best explant and best medium respectively for plant regeneration through PLBs.

Seasonal Variation in Rooting Behaviour of Ginkgo biloba L. Cuttings

Propagation by cuttings is an effective method for ex-situ conservation of plants. In the present study, propagation of Ginkgo biloba L. was conducted using hardwood and semi hard wood cuttings. The cuttings from basal part of the tree were selected for the experiment and planted during rainy (i.e. July) and summer season (i.e. April) at Central Department of Botany, T.U. Cuttings beds were prepared using 3:1 sand and soil. Different concentration of auxins such as IAA, NAA and IBA were found effective in inducing rooting. During July, the stem cuttings treated with 1000 ppm IAA for 30 minutes induced highest percentage (90 %) and statistically signifi cant (P< 0.05) rooting while those treated with same concentration and time induced only 40% rooting during April. Like wise other auxins NAA and IBA induced more rooting during July and less rooting during April. Cuttings planted as control, during July showed 10 % rooting while during April did not show any rooting. Among all the auxins studied, IAA hormone and rainy season was found to be the most effective for rooting of G. biloba cuttings. From the anatomical study it was revealed that vascular cambium, secondary phloem, cortical cells and pericycle cells took part on rooting. Successfully rooted cuttings were then transferred to the natural soil condition. J. Nat. Hist. Mus. Vol. 26, 2012: 175-180

Growth Yield and Yield Attributes of Tomato (Lycopersicon esculentum Mill.) as Influenced by Indole Acetic Acid

The experiment was conducted at research field and laboratory of the Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh during the period of December 2013 to April 2014. The experiment was laid out in two factors randomized complete block design with three replications including three concentrations of Indole Acetic Acid (0, 100 and 200 ppm) and three tomato varieties (BARI tomato 7, Manik and Ratan). Plant height, number of leaves and number of branches, days required for first flower initiation, days required for 50% flowering, days required for fruit setting, fruit cluster plant-1, fruit plant-1, weight tomato-1, yield plant-1, yield plot-1 and yield hectare-1 were significant influenced by the combined application of IAA and varieties of tomato. BARI Tomato-7 had the highest fruit yield with 100 ppm IAA and the lowest yield was observed in Ratan with 0 ppm IAA. IAA treated plots showed better performance for growth parameters and yield compared to control condition and 100 ppm IAA was more suitable than the 200 ppm IAA for higher yield of tomato cultivation. Among the treatment combinations, BARI Tomato-7 with 100 ppm IAA was superior, Ratan with 0 ppm IAA was inferior and BARI Tomato-7 with 200 ppm IAA, Manik with 200 ppm IAA and Ratan with 200 ppm IAA treated plots showed the intermediate results for yield and yield components.J. Environ. Sci. & Natural Resources, 8(1): 139-145 2015