Power-time curves and metabolic properties of Tetrahymena thermophila BF5 exposed to different Yb3+ levels were studied by ampoule method of isothermal calorimetry at 28°C. Metabolic rate (r) decreased significantly while peak time (PT) increased with the increase of Yb3+. These results were mainly due to the inhibition of cell growth, which corresponded to the decrease of cell number obtained by cell counting. Compared with cell counting, calorimetry was sensible, easy to use and convenient for monitoring the toxic effects of Yb3+ on cells and freshwater ecosystem. It was also found that cell membrane fluidity decreased significantly under the effects of Yb3+, which indicated that Yb3+ could be membrane active molecules with its effect on cell membranes as fundamental aspect of its toxicity.
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