Dietary Powder Research Articles

The effects of kelp powder and fucoidan on the intestinal digestive capacity, immune response, and bacterial community structure composition of large yellow croakers (Larimichthys crocea)

Feed terrestrial components can induce intestinal stress in fish, affecting their overall health and growth. Recent studies suggest that seaweed products may improve fish intestinal health. In this experiment, three types of feed were prepared: a basic diet (C group), a diet with 0.2 % fucoidan (F group), and a diet with 3 % kelp powder (K group). These diets were fed to large yellow croaker (Larimichthys crocea) over an 8-week period. Each feed was randomly assigned to three seawater cages (4.0 m × 4.0 m × 5.0 m) containing 700 fish per cage. The study assessed changes in growth and intestinal health, including intestinal tissue morphology, digestive enzyme activities, expression of immune-related genes, and bacterial community structure. Results showed that incorporating seaweed products into the diet improved the growth and quality traits of large yellow croakers and significantly enhanced their intestinal digestive capacity (P < 0.05). Specifically, the 0.2 % fucoidan diet significantly increased the intestinal villus length and the activities of digestive enzymes such as trypsin, lipase, and α-amylase (P < 0.05). The 3 % kelp powder diet significantly enhanced the intestinal crypt depth and the activities of trypsin and lipase (P < 0.05). Both seaweed additives significantly enhanced intestinal health by mitigating inflammatory factors. Notably, the control group's biomarkers indicated a high presence of potential pathogenic bacteria, such as Streptococcus, Pseudomonas, Enterococcus, Herbaspirillum, Neisseria, Haemophilus, and Stenotrophomonas. After the addition of seaweed additives, these bacteria were no longer the indicator bacteria, while the abundance of beneficial bacteria like Ligilactobacillus and Lactobacillus increased. Significant reductions in the expression of inflammatory factors (e.g., il-6, tnf-α, ifn-γ in the fucoidan group and il-8 in the kelp powder group) further supported these findings. Our findings suggested that both seaweed additives helped balance intestinal microbial communities and reduce bacterial antigen load. Considering the effects, costs, manufacturing, and nutrition, adding 3 % kelp powder to the feed of large yellow croaker might be preferable. This study substantiated the beneficial effects of seaweed on the aquaculture of large yellow croaker, particularly in improving intestinal health. These findings advocated for its wider and more scientifically validated use in fish farming practices.

Powder diet exacerbates oropharyngeal candidiasis in a mouse model.

This study reports on the influence of a powder diet in a mouse model of oropharyngeal candidiasis (OPC), a significant health concern caused primarily by Candida albicans. Despite identical nutritional composition, we found that a powdered diet significantly increased Candida burdens and oral lesions, and aggravated weight loss compared to a standard pelleted diet. High fungal burdens and severe oral lesions were accomplished within 48 hours after infection with only one dose of cortisone. Moreover, mice on a powder diet recovered a week after infection. Using a powder diet, we thus modified the cortisone OPC murine model in a way that simplifies the infection process, enhances reproducibility, and facilitates studies investigating both pathogenesis and recovery processes. Our findings also underscore the pivotal role of the physical form of the diet in the progression and severity of oral Candida infection in this model. Future research should investigate this relationship further to broaden our understanding of the underlying mechanisms, potentially leading to novel prevention strategies and improved disease management.IMPORTANCEOropharyngeal candidiasis (OPC) is a multifactorial disease and a significant health concern. We found that the physical form of the diet plays a critical role in the severity and progression of OPC. We developed a modified cortisone OPC murine model that facilitates studies investigating pathogenesis and recovery processes.

Determination of energy values in pistachio shell powder and soybean hulls fed to gestating and lactating sows.

Pistachio shell powder is a high-fiber co-product from the pistachio nut industry that may provide energy and nutrients in animal diets, but no data have been reported for the nutritional value of pistachio shell powder when fed to pigs. Two experiments were, therefore, conducted to test the hypothesis that apparent total tract digestibility (ATTD) of gross energy (GE), dry matter (DM), and total dietary fiber (TDF) and concentration of digestible energy (DE) in pistachio shell powder are not different from those in soybean hulls when fed to gestating or lactating sows. In experiment 1, 24 gestating sows were housed in metabolism crates and fed a corn-based basal diet or 2 diets that contained corn and 20% pistachio shell powder or corn and 20% soybean hulls. Sows were fed experimental diets for 13d with feces and urine being quantitatively collected for 4d after 6d of adaptation. In experiment 2, 24 lactating sows were housed in farrowing crates and fed a diet based on corn and soybean meal (SBM) or 2 diets that contained corn, SBM, and 20% of either pistachio shell powder or soybean hulls, and feces were collected for 6d after 7d of adaptation to the diets. Results indicated that for gestating sows, the diet containing soybean hulls had greater (P < 0.05) ATTD of DM, GE, and TDF than the diet containing pistachio shell powder. The DE and metabolizable energy (ME) in the pistachio shell powder diet were less (P < 0.05) than in the basal diet and the diet containing soybean hulls. The ME in pistachio shells (2,606 kcal/kg DM) was less (P < 0.05) than in soybean hulls (3,645 kcal/kg DM). When fed to lactating sows, ATTD of DM, GE, and TDF in the diet containing pistachio shell powder was less (P < 0.05) than in the diet containing soybean hulls or in the basal diet. The DE in the diet containing pistachio shell powder was also less (P < 0.05) than in the soybean hulls diet. The DE in pistachio shell powder (1,664 kcal/kg DM) was less (P < 0.05) than in soybean hulls (2,795 kcal/kg DM). In conclusion, the ATTD of DM and GE and the DE in pistachio shell powder were less than in soybean hulls, and inclusion in lactation diets, therefore, needs to be limited.

Growth Enhancement in Labeo rohita by replacing Conventional feed with Moringa Leaves (Moringa oleifera) Powder

Aquaculture enhancement is the need of time to encounter the food demand of the tremendously increasing population. Conventional animals and plants are equally important for human consumption, so examination of other little-researched nutritious plants such as Moringa oleifera (Lam) is necessary as an alternative source of aquaculture food protein that may be cost-effective. Objectives: To investigate the growth response of Labeo rohita species with the addition of M. oleifera leaves in conventional food and assessment of economic return and prospects to increase the farmer’s income. Methods: The current study was planned to observe the growth response and biomass production in Labeo rohita (Rohu) with increased economic returns while feeding at different doses of MOL (i-e. 0%, 10%, and 20% inclusion in conventional feed using MOL). The present study was designed in earthen ponds at Wains Agricultural Farm, UC-52, Shah Sadiq Nehang, Shorkot tehsil, Jhang district, Pakistan for 10 weeks (February to April 2018). Results: It was observed that a 10% inclusion of MOL powder in conventional fishmeal produce batter biomass as compared to other doses. At the end of the 10th week, L. rohita produced 65.16 g body weight with 10% inclusion whereas a 63.20 g increase was observed with a conventional diet. Conclusions: It was inferred that aggregate saving was about 13% when fish was fed with MOL powder diet. Thus, the present study proved a cost-effective approach for fish farming in developing countries like Pakistan.

Comparative evaluation of A1A2 and A2A2 cow milk-containing diets on biochemical and histological parameters of Wistar rats.

This Research Communication aims to compare the effect of A1A2 and A2A2 cow milk diets on the biochemical and histological parameters of rats. The rats were divided into four groups and fed with a normal diet, A2 milk powder, A1A2 or A2A2 cow milk diets for 90 d. Blood glucose, kidney function, liver function and lipid profile were examined during the experimental period. The study showed an increase in the body weight of the A1A2 group whereas a slight decrease in the A2A2 group, and blood glucose levels increased from d 0 to day 90 in all experimental groups. However, none of these changes were found to be statistically insignificant (P > 0.05). Moreover, no significant changes were recorded in other parameters (serum glutamic pyruvic transferase and serum glutamic-oxaloacetic transaminase for liver function, bilirubin direct, cholesterol, triglycerides, creatinine and uric acid). The histology of the liver, kidney and pancreas also showed no changes in all groups. Overall, this study revealed no significant difference in the nutritional values of A1A2 and A2A2 milk types and hence equally beneficial for health. Although the present study showed no significant difference in the effect of both milk types in 90 d, further studies might be conducted to evaluate their longer term effects.

Effect of a Baobab Powder Diet on Growth and Digestive Balance in Growing Rats

Introduction: The fight against malnutrition (over- and undernutrition) requires knowledge of the fruits we eat. Baobab is a fruit from the north of Côte d'Ivoire whose consumption can help improve nutrition. Aim: The aim of this study was to investigate the chemical composition of baobab pulp and evaluate its nutritional impact after consumption in growing rats. Methodology: To achieve this objective, the physicochemical parameters and macronutrient contents of baobab flour were determined. Then, 3 batches of 5 rats were fed separately with three diets: RFB (baobab pulp), RM (baobab pulp + cornstarch) and RFM (cornstarch). Regular weighing was carried out to determine growth and digestibility parameters. Results: At the end of the work, it was found that baobab pulp is an acidic food (pH = 3.2), composed mainly of carbohydrates (92.47), mainly energy. It has a high starch content (56.34), a high sugar content (21.37), hence its sweet taste; with a considerable fiber content (7.82), in terms of dry matter (DM). Baobab flour contains very low levels of protein (2.08) and lipids (0.37). From a nutritional point of view, the baobab pulp diet (RFB) does not promote rat growth, and therefore the utilization of dietary protein. In addition, the RFB diet results in negative digestibility of dietary proteins and very low digestibility of the dry matter in the food consumed. On the other hand, rat growth and the digestibility of dietary protein and dry matter increased with the reduction (RM) or elimination (RFM) of baobab flour from the diet. Conclusion: Ultimately, these results enable us to assess the nutritional quality of baobab flour. Its consumption, which does not promote weight gain despite its high carbohydrate content, can therefore be used in the fight against obesity.

Liver triacylglycerol accumulation but not postprandial lipemia is reduced by a skim milk powder diet in male rats

Increases in postprandial lipids are linked to the development of cardiometabolic and fatty liver disease. Prior work has suggested that dairy possesses beneficial cardiometabolic effects and thus the aim of the current investigation was to test the hypotheses that the habitual consumption of dairy, in the form of skim milk powder (SMP), would protect against increases in circulating lipids and liver lipid accumulation following an oral fat challenge in rats. Male rats were fed either a semipurified low-fat control diet with casein or a diet with an equivalent amount of protein (∼13% kcal) provided through skim milk powder (SMP) for 6 weeks (n = 40/group). Rats were then given an oral gavage of palm oil (5 mL/kg body weight) or an equivalent volume of water, and serum and liver were harvested 90 minutes or 4 hours after. Rats fed the SMP diet gained less weight than controls but there were no differences in glucose tolerance between groups. The fat gavage increased serum lipids in both diet groups, whereas there was a main effect of the fat challenge to increase, and the SMP diet, to decrease liver triacylglycerol accumulation. The percentage of saturated and monounsaturated fatty acids and the protein content/activity of lipogenic enzymes were reduced in livers from SMP-fed rats, whereas the percentage of polyunsaturated fatty acids was increased. In summary, we provide evidence that SMP consumption, although not protecting against postprandial lipemia, markedly attenuates triacylglycerol accumulation and the relative amount of saturated and monounsaturated fatty acids in the liver.

Alternate Day Feeding as a Cost-effective Strategy for Tank Culture of the Pacific Shortfin Eel Anguilla bicolor pacifica

There is a growing interest in the aquaculture of tropical anguillid eels as an export commodity. However, studies on feeding strategies, and the present demand to reduce feed costs need to be addressed to ensure the economic viability of eel farming. In this study, the effects of daily (DF) and alternate day (ADF) feeding on growth, feed utilization, body composition, blood chemistry, liver and intestinal morphology, and economic viability in the Pacific shortfin eel Anguilla bicolor pacifica were examined. Each feeding group of 30 elvers (166.25 ± 16.23 g mean initial wt) were randomly stocked in triplicate 4 m3 outdoor concrete tanks. These were fed for 155 d with formulated eel powder diet (49.77% crude protein; 10.21% crude lipid) made into a paste. Growth and survival were not significantly different between the two feeding groups. However, feed efficiency was improved in the ADF group, with significantly higher protein efficiency ratio (0.63) than in the DF group (0.39). Feed conversion ratio (FCR) was lower in ADF (3.85) than in the DF group (6.44), resulting in a 40% reduction in total feed consumption. Biometric indices, body proximate composition, and blood chemistry were not significantly affected. Likewise, liver and intestinal morphology showed no apparent alterations between groups. Partial costs-and-returns analysis showed that ADF yielded higher net profit and profit index. These results suggest that feeding on alternate days promote compensatory growth, better feed utilization, and normal physiological condition of A. bicolor pacifica, and reduce feed cost in the rearing process. Therefore, alternate day feeding should be promoted as a sound feed management strategy in the tank culture of tropical anguillid eels.

Effects of roasted burdock root tea, drink, and the residue on caecal microbiota of mice fed low-dietary fibre diet

Burdock (Arctium lappa) root has been traditionally consumed in Japan since ancient times. Roasted burdock tea (BT) is gaining popularity worldwide as a health drink as it is rich in inulin and chlorogenic acid, and has been reported to have health benefits, including inhibitory and ameliorative effects on constipation and bowel inflammation. However, the underlying mechanism remains unclear. To determine the effects of BT on gut microbiota, diet without cellulose (no fibre: NF), 5% (w/w) whole roasted burdock root powder (WB) diet, 5% (w/v) BT as drinking liquid, or 1.7% (w/w) residue of BT (BR) diet was fed to mice. Microbiota analysis was performed using 16S rRNA (V4) gene amplicon sequencing. The abundance of microbial groups related to obesity and inflammation, such as Allobaculum, was decreased by feeding burdock root preparations, particularly BT (0.6%) compared with NF (25%). Conversely, the abundance of Muribaculaceae, which are gut commensals that correlate with anti-inflammation, immunomodulation, and longevity, in the three burdock groups was 2–3-times higher than that in the NF group. The levels of tumour necrosis factor-α, a pro-inflammatory cytokine, were 2.5-fold decreased in the BT group. In conclusion, BT may affect gut microbiota composition and, thereby, impact the host immune system.

Comparative effect of various powder diets on growth of big‐eyed bug Geocoris ochropterus (Fieber) (Hemiptera: Geocoridae)

AbstractApplication of powder diets may promote development of the predator Geocoris ochropterus (Fieber) when densities are low in greenhouses or fields. Development and nymphal survival ratio of G. ochropterus fed on different powder diets including silkworm pupae (A), crickets (B), silkworm pupae and sunflower seed (C) and crickets and sunflower seed (D) were examined. Total average development period was 30.14 days for instars fed on silkworm pupae, 34.43 days for instars fed on crickets, 26.28 days for instars fed on silkworm pupae and sunflower seed and 29.67 days for instars fed on crickets and sunflower seed, and they were significantly different. Head width, body length, forewing length, dry body weight of adults and sex ratio were not affected by the different diets. There were significantly different found in survival rates among the diets when the nymph reached 5th instar. Results indicate that the combination powder of silkworm pupae and sunflower seed will promise as an alternative diet in the greenhouses to maintain the population of G. ochropterus.

A SIMPLE MASS REARING METHOD FOR BRINJAL SHOOT AND FRUIT BORER &lt;i&gt;LEUCINODES ORBONALIS&lt;/i&gt; GUENEE

A simple, effective and economically cheaper method for mass rearing of the brinjal shoot and fruit borer, Leucinodes orbonalis Guenee was developed using both modified artificial diet fortified with brinjal powder and natural diet - potato. A comparison of the various biological parameters such as egg incubation period, larval period, larval weight (12-day old), pre-pupal period, pupal period, pupal weight (1-day old), adult longevity, fecundity, oviposition period and sex ratio of different life stages of the test-insect were studied for both the food sources in this investigation. The larval and pupal periods on natural (potato) diet were 14.58± 1.51 and 6.7± 1.22 days respectively, with a mean fecundity of 165.55± 36.26 per female and a mean life cycle of 26.91days. The larval and pupal period of shoot and fruit borer reared on modified diet were 15.97± 1.24 and 6.25± 1.23 days respectively with a mean fecundity of 149.45± 25.83 per female and a mean life cycle of 25.26 days. Provision of a suitable site for adult mating and oviposition was attained by designing a separate mating chamber along with a suitable egg laying substratum. Thus, an easy and economical rearing technique could successfully support the rearing to six generations of target-insect in laboratory.

Dietary powder and molecular imprinted polymer nanoencapsulated sodium propionate to enhance growth performance, digestive enzymes activity, antioxidant defense, and mucosal immune response in African cichlid (Labidochromis lividus) fingerlings

Abstract This study was conducted to examine the effects of powder sodium propionate (P-SP) and SP- loaded molecular imprinted polymer (MIP) nanoparticles (MIP-SP NPs) on the growth, skin mucosal immune parameters, and digestive and liver enzymes activities of African cichlid (Labidochromis lividus) fingerlings. Fish with an average weight of 500±2 mg were stocked into 12 experimental units and fed on experimental diets prepared by supplementing the basal diet (control) with MIP NPs, P-SP (5 g SP kg−1 of dry diet), and MIP-SP NPs for 8 weeks. The findings demonstrated that growth indices improved in the MIP-SP NPs followed by the P-SP dietary group compared to the control groups (P&lt;0.05). The activity of digestive enzymes of lipase, trypsin, protease, and alkaline phosphatase was higher in the fish fed SP-supplemented diets than in the controls (P&lt;0.05). The protease and lipase activities in the MIP-SP NPs dietary group increased by 29.33% and 48.81% compared to the control, respectively (P&lt;0.05). In addition, the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of liver tissue decreased in the SP dietary groups, while the catalase (CAT ), superoxide dismutase (SOD), and alkaline phosphatase (ALP) levels increased compared to the control groups (P&lt;0.05). The highest SOD and ALP levels were observed in the fish fed on the MIP-SP NPs-supplemented diet (P&lt;0.05). Furthermore, the skin mucosal immune indices, including alternative hemolytic complement activity (ACH50), lysozyme, and total immunoglobulin (Ig) levels increased in the MIP-SP NPs and P-SP dietary groups compared to the controls (P&lt;0.05). The findings indicated that sodium propionate encapsulated in molecularly imprinted polymer nanoparticles could enhance the efficiency of dietary SP in African cichlid fish.

Membrane filtration processing of infant milk formula alters protein digestion in young pigs

Reducing heat treatment (HT) during processing of infant milk formula (IMF) is desirable to produce a product that more closely resembles breast milk. By employing membrane filtration (MEM), we produced an IMF (60:40 whey to casein ratio) at pilot scale (250 kg). MEM-IMF had a significantly higher content of native whey (59.9 %) compared to HT-IMF (4.5 %) (p < 0.001). Pigs, at 28 days old, were blocked by sex, weight and litter origin and assigned to one of two treatments (n = 14/treatment): (1) starter diet containing 35 % of HT-IMF powder or (2) starter diet containing 35 % of MEM-IMF powder for 28 days. Body weight and feed intake were recorded weekly. Pigs at day 28 post weaning were sacrificed 180 min after their final feeding, for the collection of gastric, duodenal, jejunum and ileal contents (n = 10/treatment). MEM-IMF diet resulted in more water-soluble proteins and higher levels of protein hydrolysis in the digesta at various gut locations compared to HT-IMF (p < 0.05). In the jejunal digesta, a higher concentration of free amino acids were present post MEM-IMF consumption (247 ± 15 µmol g−1 of protein in digesta) compared to HT-IMF (205 ± 21 µmol g−1 of protein). Overall, average daily weight gain, average dairy feed intake and feed conversion efficiency were similar for pigs fed either MEM-IMF or HT-IMF diets, but differences and trends to difference of these indicators were determined in particular intervention periods. In conclusion, reducing heat treatment during processing of IMF influenced protein digestion and revealed minor effects on growth parameters providing in vivo evidence that babies who are fed with IMF processed by MEM are likely to have different protein digestion kinetics but minimal effect on overall growth trajectories as babies fed IMF processed by traditional thermal processing.

Effect of Diet Supplemented with Graded Levels of Ginger (Zingiber officinale) Powder on Growth Performance, Hematological Parameters, and Serum Lipids of Broiler Chickens.

The present study was conducted at the Poultry Research Center, Faculty of Agriculture; Sebha University, Libya. The feeding trial lasted 42 days in total. A local hatchery provided 240 unsexed one-day-old broiler chicks (Ross 308). The birds were randomly distributed into four groups, and each group was then divided into four replicates, with 15 birds in each group. The objective of the current study was to examine the impact of ginger powder as a natural feed supplement on broiler chickens' growth performance, hematological parameters, and serum lipids. Four dietary treatments were formulated and supplemented with ginger powder at levels 0%, 0.2%, 0.4% and 0.6%. The findings exhibited substantial differences in body weight and weight gain across the experimental groups. Birds fed ginger-enriched diets had higher feed conversion and lower feed intake than control birds (P<0.05). Birds fed a 0.6% ginger powder diet compared to the control group had considerably superior productive performance (P<0.05), while feed consumption was lowered. Dietary ginger powder supplementation improved hematological parameters; white blood cells, lymphocytes, heterophils, and the lymphocyte to heterophil ratio were improved with dietary ginger powder supplementation (P<0.05). Additionally, as compared to the control group, ginger powder groups significantly (P<0.05) lowered serum triglycerides, total cholesterol, and very low-density lipoprotein. While supplementation of dietary ginger powder significantly increased (P<0.05), the levels of high-density lipoprotein against the control group. In conclusion, ginger supplementation had a positive effect on the performance of chickens when supplemented on broiler ration and can be considered an alternative growth promoter in feeding broiler ration.

The effect of a siamese pumpkin and tuna-based powder (CHAGURO) diet in improving chol/HDL ratios in prediabetes individuals with dyslipidemia: a randomised trial

Background: The study aimed to examine the effect of administering CHAGURO (a product of the combination of siamese pumpkin and tuna-based powder) on the lipid profile in prediabetes individuals with dyslipidemia. Methods: The experimental trial was conducted with two intervention groups, with each group receiving a dose of 7.5g/70kg body weight (BW)/day (low dose) and 15g/70kg BW/day (high dose) for 60 days. Respondents in each group ranged in age from 30 to 60 years old. Prediabetes was defined as fasting blood sugar levels of 100-125 mg/dL, while cholesterol levels of 200 mg/dL was considered dyslipidemia. The mean baseline HbA1c level for all subjects was 5.5%. Respondents were randomized into intervention groups using simple randomization. Pre-test and post-test measurements were performed for body mass index (BMI), physical activity (using IPAQ-SF), hemoglobin A1c (HbA1c), lipid profile, blood pressure, and food intake (using SQ-FFQ). Statistical analyses performed included descriptive tests, independent and paired T-tests, and analysis of variance (ANOVA). Results: Compared to the baseline, HbA1c, total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides in the two groups did not improve significantly by 60 days. For lipid variables, a significant improvement in the cholesterol/high-density lipoprotein (chol/HDL) ratios were observed when compared against the baseline for the two groups. When comparing the changes between groups, 7.5 g of CHAGURO showed a larger improvement in HDL-C (mean differences: 1.86 mg/dL; 95% CI: -0.86-4.59), compared to 15 g of CHAGURO (mean differences: -3.62 mg/dL; 95% CI: -7.53-0.28). A significant (p&lt;0.05) decrease in the chol/HDL ratio was observed in the 7.5 g CHAGURO group (mean differences: -0.20 mg/dL; 95% CI: -0.39-(-0.01)). Conclusions: CHAGURO can be a functional food alternative to improve the condition of prediabetes people with dyslipidemia. Trial registration: Dr. Sardjito General Hospital (KE/FK/0951/EC/2020), 27/08/2020

Preventive effects of garlic powder on cisplatin-induced nephrotoxicity in male wister rats

Cisplatin is cancer chemotherapeutic agent that used in treatment of many types of solid cancers. However, cisplatin administration showed adverse effects including multi-organs toxicity including nephrotoxicity. Garlic is an anti-carcinogenic, antioxidant, anti-diabetic, Reno protective, anti-atherosclerotic, antibacterial, antifungal, and antihypertensive agent. Therefore, present study was conducted to investigate preventive effect of garlic powder on Cisplatin induced nephrotoxicity in male Wister rats. Twenty-one male Wister rats were assigned into three groups namely, C (as control negative group), CP (control positive) received two i.p injection of cisplatin at dose of 8 mg/kg and G-CP received two i.p injection of cisplatin at dose of 8 mg/kg and fed with 10% garlic powder diet. Serum samples were collected and kidney function parameters including creatinine, urea and uric acid levels were measured using colorimetric kits for these parameters. creatinine results showed a significant increasing in CP group rats compared with G-CP group. Urea results showed a significant increasing in CP group rats compared with G-CP group. Also, uric acid results showed a significant increasing in CP group rats compared with G-CP group. In conclusion, rats that fed with 10% garlic powder diet reduced nephrotoxicity that induced by Cisplatin.

EFFECT OF 3 MONTHS-FEEDING OF A POWDER DIET FORTIFIED WITH CHILLI (GUNTUR, BIRD’S EYE, AND NAGA KING) ON SUPEROXIDE DISMUTASE LEVELS IN SD RAT

Purpose: Chilli has been considered a culprit and saviour in various non-communicable disorders in the scientific world. We hypothesise that varying proportions of active principles in different chilli affect its therapeutic potential significantly, and it could be a game-changer in the area of drug discovery. Design/methodology/approach: In this regard, nutritional analysis was performed for three chillies, followed by the animal study on SD rats fed a standard rodent powder diet (20% protein) fortified with 0.005% of capsaicin (CAP) equivalent chilli powder for three months to measure superoxide dismutase (SOD) in five visceral organs/tissues. Finding: The NKC and BEC groups have shown opposite results to the GC and capsaicin equivalent groups (CEG) regarding animal body weight and SOD levels in various tissues. However, there is no change in feed intake. Research limitations/implications: In parallel, serum SOD should have been tested. Other methods for SOD analysis would fetch confirmatory results. Using a mild or moderate stress rodent model would prove its therapeutic efficacy. CAP alone might affect anti-obesity and SOD levels; however, the DHC content of chilli plays an essential role in providing synergistic or antagonistic effects on anti-obesity or activating the antioxidant system depending on the threshold level. It means it could have biphasic properties similar to capsaicin. Originality/value: The present paper generates evidence that capsaicinoid relative proportion in chilli plays an essential role in its therapeutic or negative impact on health. KEYWORDS: Naga King Chilli, Guntur Chilli, Bird’s Eye Chilli, Capsaicin, Wistar rat Paper type: Original article

Monocaprin Enhances Bioavailability of Fucoxanthin in Diabetic/Obese KK-Ay Mice.

Fucoxanthin is a marine carotenoid found in brown seaweeds and several microalgae. It has been reported that fucoxanthin has health benefits such as anti-obesity and anti-diabetic effects. To facilitate fucoxanthin applications in the food industry, it is important to improve its low bioavailability. We attempted the combined feeding of fucoxanthin-containing seaweed oil (SO) and monocaprin in a powder diet and analyzed the fucoxanthin metabolite contents in the liver, small intestine and serum of diabetic/obese KK-Ay mice. After 4 weeks of feeding with the experimental diets, the serum fucoxanthinol concentrations of the mice fed 0.2% SO and 0.5% monocaprin were higher than those of the 0.2% SO-fed mice. Furthermore, fucoxanthinol accumulation in the liver and small intestine tended to increase in a combination diet of 0.2% SO and 0.125–0.5% monocaprin compared with a diet of 0.2% SO alone, although amarouciaxanthin A accumulation was not different among the 0.2% SO-fed groups. These results suggest that a combination of monocaprin with fucoxanthin-containing SO is an effective treatment for improving the bioavailability of fucoxanthin.

Abstract LB165: Preclinical evaluation of carnosic acid (CA) and rosemary extract standardized to 40% carnosic acid (RE40CA) for the prevention of invasive breast cancer

Abstract Background: There is an unmet need for the development of safer and more effective therapies for the prevention of human invasive breast cancer, for which ductal carcinoma in situ (DCIS) is a precursor. Currently, anti-hormonal therapies are the only option in individuals previously diagnosed with hormone receptor positive breast cancer. However, these agents are associated with many side effects. Furthermore, there are currently no preventive options for hormone receptor negative breast cancers. To study DCIS progression, we developed a model referred to as MIND (Mouse-INtraDuctal) in which patient-derived DCIS cells and cell lines are injected intraductally and studied as they progress to invasive ductal carcinoma (IDC). Previously we provided strong evidence for the role of B Cell Lymphoma-9 (BCL9) as a potential driver of DCIS malignancy. We chose to target BCL9 using RE40CA and its active ingredient CA previously identified in a screen for inhibitors of β-catenin binding to BCL9. Experimental Strategy: CA and RE40CA were administered to DCIS.COM MIND animal models for four weeks by oral gavage or mixed in a powder diet formulation. Drug plasma levels were evaluated by LC-MS. Four weeks following drug administration, mammary glands were excised and evaluated for efficacy and expression of biomarkers of aggressive DCIS, nuclear β-catenin and BCL9. For efficacy studies, mammary glands containing DCIS like lesions were processed and subjected to immunofluorescence staining using anti-human CK-5, anti-SMA, Dapi, β-catenin and BCL9. Extent of DCIS in vivo growth, number of invasive lesions, tumor number and tumor volume were evaluated for each mammary gland. Results: Efficacy studies showed a significant reduction in the number of invasive lesions by administration of CA by oral gavage (CA OG:10mg/kg), CA at 200mg/kg diet (CA200), CA400 and RE40CA 500. A significant reduction in extent of DCIS in vivo growth was achieved by CA OG and RE40CA 500. A significant reduction in the number of tumors was achieved by CA OG, RE40CA OG (25mg/kg equivalent to CA 10mg/kg), RE40CA 100, RE40CA 500 and RE40CA 1000. A significant reduction in tumor volume was achieved by CA OG, RE40CA OG, RE40CA 100, RE40CA 500 and RE40CA 1000. Linear regression modelling was used to compare statistical differences among the groups and differences that reached p-value &amp;lt;0.05 were considered significant. Administration of CA or RE40CA by oral gavage was also associated with a significant loss of nuclear BCL9/β-catenin expression in DCIS epithelial cells. Dietary administration of CA and RE40CA as OG or mixed in powder diet showed an increasing trend in plasma concentrations as the dose was increased. Evaluation of HE stained sections of animal organs by histopathology showed no evidence in any tissue of necrosis, degeneration, inflammation or neoplasia. Additionally, no consistent lesions attributable to treatment were observed in any of the animals or groups. Conclusion: Administration of CA and RE40CA are safe and effective in prevention of DCIS malignancy. Future studies are aimed at testing the efficacy of CA/RE40CA using DCIS cell lines representing other DCIS subtypes (ER+/PR+ and HER2 overexpressing) as well as patient derived DCIS MIND animal models. Supported by NCI PREVENT Cancer Preclinical Drug Development Program, 75N91019D00016/75N91020F00001. Citation Format: Yan Hong, Aditi Rastogi, Aryamitra Banerjee, Brandon L. Plattner, Matthew Lindeblad, Alexander V. Lyubimov, Timothy Fields, Seema A. Khan, Altaf Mohammed, Jeremy J. Johnson, Fariba Behbod. Preclinical evaluation of carnosic acid (CA) and rosemary extract standardized to 40% carnosic acid (RE40CA) for the prevention of invasive breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB165.

Influence of green tea powder on the performance, nutrient utilisation, caecal microbiota profile and meat quality of broiler chickens

This study assessed the influence of green tea powder diet supplementation on performance, nutrient utilisation, caecal microbiota profile and meat quality in broiler chickens. A total of 144, one-day-old broiler (Ross 308) chicks were allocated to 18 cages (eight broilers/cage) which were randomly assigned to one of three dietary treatments: a wheat-based basal diet or supplemented with one of two types of green tea; normal (N-GT) or selenium-rich (Se-GT) tea, at an inclusion rate of 1%. Bird performance, nitrogen-corrected apparent metabolisable energy (AMEn), and total tract digestibility of fat and starch were measured on d 7, 21 and 35. Effects on microbiota profile on d 7, 21 and 35 were determined in a cohort study. Green tea supplementation reduced (P&lt;0.05) the weight gain and feed intake but improved (P&lt;0.05) feed efficiency. Supplementation with N-GT increased the AMEn on d 7 and 21, and with Se-GT on d 7 (P&lt;0.05). AMEn increased with age for all treatments. Fat digestibility increased (P&lt;0.05) in birds fed N-GT on d 21 and Se-GT on d 7 and 21. Starch digestibility increased (P&lt;0.05) on d 21 with N-GT supplementation and on d 7 with Se-GT supplementation. Carcass and breast meat yields were unaffected (P&lt;0.05) by the dietary treatments. The abdominal fat pad decreased (P&lt;0.05) in the N-GT diet and numerically in the Se- GT diet. Drip loss was less in meat from birds (P&lt;0.05) fed both green tea treatments. Cooking loss was reduced in the Se-GT treatment (P&lt;0.05). Dietary inclusion of green tea powder positively influenced microbiota profile, with increased (P&lt;0.05) numbers of beneficial bacteria (Lactobacillus spp. and Bifidobacterium spp.) and reduced (P&lt;0.05) numbers of pathogenic bacteria (Clostridium spp. and Bacteroides spp.). Differences were observed between the two green tea types, with Se-GT being more beneficial than N-GT in the positive modulation of intestinal microbiota.