Potassium Tetraborate Research Articles

Sustainable release of borate- and nitrate- electrolyte additives via metal-organic frameworks nanocapsules for stable lithium metal batteries

Constructing robust electrolyte interphase through inorganic electrolyte additives is an effective strategy to improve the electrochemical performance of lithium metal batteries (LMBs). However, the limited solubility of such additives in electrolyte hinders their further application. Herein, we apply metal–organic frameworks (MOFs) as nanocapsules to sustainably release lithium nitrate (LiNO3) and a novel salt of potassium tetraborate (K2B4O5(OH)4). LiNO3 is inclined to undergo reduction on lithium anode to form a nitride-rich solid electrolyte interphase (SEI) layer; while K2B4O5(OH)4 preferentially decomposes on high-voltage cathodes to create a boron-rich cathode electrolyte interphase (CEI) layer. Although both salts exhibit poor solubility in commercial carbonate electrolyte, they can be continuously consumed on the electrode surface and concurrently replenished from the nanocapsules during long-term cycling process. As a result, a compact lithium deposition is achieved and the dissolution of transition metals is restrained. Upon the sacrifice of LiNO3 and K2B4O5(OH)4, the lifespan of LMBs is remarkbly improved. The Li||LiNi0.5Co0.2Mn0.3 (NCM523) cells at 4.3 V and Li||LiNi0.6Co0.2Mn0.2 (NCM622) cells at 4.4 V can respectively deliver high capacity retentions of 87 % and 80.1 % for 200 cycles.

Intracellular and mitochondrial proteomic analysis reveals antifungal mechanisms of borate on mango black spot pathogen Alternaria alternata

AbstractBoron, in the form of potassium tetraborate, has previously been found to be effective at inhibiting mango black spot disease, caused by Alternaria alternata. However, the mechanisms involved in this inhibition are largely unknown. In this study, A. alternata was treated in vitro with potassium tetraborate at a concentration of 5–10 mM for 48 or 72 h. The intracellular and mitochondrial proteins were extracted from mycelium and separated using two‐dimensional electrophoresis (2‐DE). Differentially expressed proteins (DEPs) were identified using bioinformatics tools and differences between protein spots were derived from mass spectrometry (MS). Using matrix‐assisted laser desorption ionization time‐of‐flight tandem mass spectrometry (MALDI‐ToF‐MS/MS), 96 intracellular and 56 mitochondrial DEPs were identified. The intracellular proteins identified were found to be involved in posttranslational modifications, protein turnover and chaperones, while the mitochondrial proteins were involved in electron transport chains. Our results demonstrate that various metabolic pathways are involved in the antifungal activity of boron. The differential expression of 20 genes was also verified at the mRNA level by reverse transcription‐quantitative PCR. Our study suggests that borate could be used as a potential substitute for synthetic fungicides to control this postharvest disease of mango fruits.

Investigation of Potassium Tetraborate Resistance in Dickeya spp.

Dickeya spp. are common plant pathogens associated with bacterial soft rot, potato blackleg, and slow wilt, which are plant diseases that account for major losses in the agricultural industry. The diseases caused by these bacterial species are not yet fully managed with existing techniques, and new approaches need to be considered to minimize future crop loss. Previous research has shown that the inorganic salt potassium tetraborate tetrahydrate (PTB) can inhibit the growth of Dickeya species; however, disk diffusion assays result in a unique phenotype with two zones of inhibition. This study investigates the effects of PTB on the growth of four Dickeya spp.. It was hypothesized that the production of phage is responsible for the two zones of inhibition. Disk diffusion assays and growth curves were used to confirm the impact of PTB on Dickeya and attempts were made to directly isolate phage from the strains. To elucidate the mechanism of action of PTB, Tn-Seq libraries were used to determine which genes are required for growth in the presence of PTB. Tn-Seq libraries showed that different Dickeya strains shared seven overlapping genes including stress-related genes that increase bacterial resistance to PTB. Gene expression studies were used to determine the changes in gene expression that result from PTB exposure. Preliminary results showed that exposure to PTB induces the expression of stress-related genes in Dickeya to increase survival in the presence of the compound. Further research is needed to better understand the implications of observed changes in bacterial gene expression.

Carboxymethyl chitosan-derived carbon foam with hierarchical pores tuned by potassium tetraborate and potassium carbonate for supercapacitors

Porous carbons have been extensively concerned in energy-storage fields due to their good conductivity, stable chemical and physical properties, and large specific surface area. The fabrication of porous carbons possessing controlled structure and excellent capacitive performance by facile preparation methods is highly desirable. Herein, a nitrogen-doped carbon foam derived from carboxymethyl chitosan is fabricated via a gel-based method with potassium tetraborate tetrahydrate (K2B4O7·4H2O) as the template agent and potassium carbonate (K2CO3) as the activating agent. The as-prepared carbon foam (KBCF) owns hierarchical pores (i.e., macro-, meso-, and micropores) resulting from the synergy of K2B4O7 and K2CO3. Moreover, the doped nitrogen atoms in KBCF originating from the amino groups in carboxymethyl chitosan provide additional pseudocapacitance. As a result, KBCF possesses a large specific surface area (large to ~3231.0 m2 g−1), outstanding specific capacitance (317 F/g at 1 A g−1), and excellent cycle life (retaining 96.3 % of the initial capacitance after 10,000 consecutive charge-discharge cycles). The assembled symmetric quasi-solid supercapacitor (KBCF//KBCF) can deliver a high power density of 25 kW kg−1 at an energy density of 2.35 Wh kg−1 and an energy density of 6.84 Wh kg−1 at a power density of 500 W kg−1. The outstanding electrochemical performance demonstrates a promising prospect of KBCF in high-performance supercapacitor electrodes.

Эволюция спектральных и структурных характеристик боратов, образующихся при взаимодействии оксидов лантана и скандия с расплавом тетрабората калия

The structure, morphology, IR spectra, as well as luminescence spectra and luminescence excitation spectra of europium-doped borates formed during the interaction of lanthanum and scandium oxides with a potassium tetraborate melt at 970°С with the general formula La0.99-xScxEu0.01BO3 were studied. It is shown that with an increase in the Sc3+ concentration, three compounds are successively formed: LaBO3, LaSc3(BO3)4, and ScBO3. At 0 ≤ x ≤ 0.26, the samples are single-phase and have the structure of LaBO3 aragonite. Within the range of 0.26 < x < 0.75, the samples are two-phase and contain the structures of LaBO3 aragonite and LaSc3(BO3)4 huntite. At 0.75 ≤ x ≤ 0.85, LaSc3(BO3)4 is observed. Within the range of 0.85 < x ≤ 0.97, the samples are two-phase and consist of LaSc3(BO3)4 huntite and ScBO3 calcite. At 0.97 < x ≤ 0.99, the ScBO3 samples have a calcite structure. Correspondence between the structure and spectral characteristics of these compounds was established.

Эволюция спектральных и структурных характеристик боратов, образующихся при взаимодействии оксидов лантана и индия с расплавом тетрабората калия

The structure, morphology, IR spectra, as well as luminescence spectra and luminescence excitation spectra of europium-doped borates formed during the interaction of lanthanum and indium oxides with a potassium tetraborate melt at 970 °С have been studied. With an increase in the In3+ concentration in the batch, a sequential change of the following structural states is observed: single-phase LaBO3 aragonite, LaBO3 aragonite + InBO3 calcite, single-phase InBO3 calcite. The correspondence between the structure and spectral characteristics of these compounds has been established. It is shown that indium and lanthanum orthoborates do not form joint solid solutions.

Evolution of the spectral and structural characteristics of borates formed during the interaction of lanthanum and scandium oxides with a potassium tetraborate melt

The structure, morphology, IR spectra, as well as luminescence spectra and luminescence excitation spectra of europium-doped borates formed during the interaction of lanthanum and scandium oxides with a potassium tetraborate melt at 970oC with the general formula La0.99-xScxEu0.01BO3 were studied. It is shown that with an increase in the Sc3+ concentration, three compounds are successively formed: LaBO3, LaSc3(BO3)4, and ScBO3. At 0≤ x≤ 0.26, the samples are single-phase and have the structure of LaBO3 aragonite. Within the range of 0.26<x<0.75, the samples are two-phase and contain the structures of LaBO3 aragonite and LaSc3(BO3)4 huntite. At 0.75≤ x≤0.85, LaSc3(BO3)4 is observed. Within the range of 0.85<x≤0.97, the samples are two-phase and consist of LaSc3(BO3)4 huntite and ScBO3 calcite. At 0.97<x≤0.99, the ScBO3 samples have a calcite structure. Correspondence between the structure and spectral characteristics of these compounds was established. Keywords: phosphors for LEDs, rare earth orthoborates, X-ray diffraction analysis, IR spectroscopy, luminescence spectra.

A novel strategy to synthesize bioactive glass based on the eutectic reaction of B2O3–K2O

Melt-derived route was used to prepare modified bioactive glass-ceramic based on the 45S5 composition with the same network connectivity. Their phase composition, sinterability, and bioactivity were studied. A modified composition was proposed using potassium tetraborate (K2B4O7) to reduce the melting temperature during manufacture. The phase composition and the bioactivity was determined by X-ray diffraction and Fourier transform infrared spectroscopy. Furthermore, the antibacterial properties were evaluated against Enterococcus faecalis. The result shows that glass-ceramics already had P–O and C–O bond functional groups on day 2. These bonds are responsible for the creation of the HCA layer. Scanning electron microscopy (SEM) pictures and Energy Dispersive X-ray Spectroscopy (EDX) investigations showed that, after being immersed in SBF solution, a layer of hydroxyapatite (HA) formed on both BG surfaces on day 2 and that by day 21, HCA cluster crystals had developed. Inductively coupled plasma-optical emission spectroscopy metrics of ionic release from the prepared glass-ceramic, mainly calcium and phosphorus ions in SBF solution, revealed that HCA formation occurred on both BG surfaces, which correlated to the increasing pH within 2 days of incubation; furthermore, it exhibited good antibacterial behavior against the Enterococcus faecalis.

Mean activity coefficients of KCl in the KCl–K2B4O7–H2O solutions at 288.15 K determined by cell potential method and their application to the prediction of solid–liquid equilibria of the KCl–K2B4O7–H2O system

• The unreported mixed ion interaction of Pitzer parameters in KCl–K 2 B 4 O 7 –H 2 O solutions at 288.15 K were obtained in this work. • The experimental and predicted solubilities were compared to verify the rationality of fitting parameters. After measuring the cell potential of the cell without liquid junction K-ISE | KCl ( m 1 ), K 2 B 4 O 7 ( m 2 ) | Cl-ISE with the calibrated potassium ion and chloride ion selective electrode, the mean activity coefficients of KCl in KCl–K 2 B 4 O 7 –H 2 O solutions are calculated by the Nernst equation. The single parameters β (0) , β (1) , C Φ of K 2 B 4 O 7 , and the mixed ion interaction parameters θ Cl, B 4 O 5 (OH) 4 and ψ K,Cl, B 4 O 5 (OH) 4 of the Pitzer model were determined by programming solver and mean activity coefficients in this work. Furthermore, Pitzer equation is also used to evaluate the mean activity coefficient of potassium tetraborate in the KCl–K 2 B 4 O 7 –H 2 O solutions, as well as the osmotic coefficients, water activity and excess Gibbs free energy of the mixed solutions. Additionally, the experimental measurements of phase equilibria in the ternary system KCl–K 2 B 4 O 7 –H 2 O at 288.15 K are investigated in this work. The solubilities of the salts in the ternary system at 288.15 K are predicted by using Pitzer equation and the obtained ion parameters. The comparision of experimental and calculated phase diagram is presented in detail. The prediction results show that the predicted data are in accordance with the experimental data.

Evaluation of potassium borate as a volatility-reducing agent for dicamba

AbstractDicamba was labeled in dicamba-resistant cotton (Gossypium hirsutum L.) and soybean [Glycine max (L.) Merr.] in 2017, resulting in a record number of off-target complaints. To address off-target movement via volatilization, experiments were conducted to evaluate the effectiveness of potassium tetraborate tetrahydrate (KBo) as a volatility-reducing agent (VRA) with dicamba. Low-tunnel experiments examined: (1) whether KBo functions as a dicamba VRA, (2) the relationship between KBo concentration and dicamba volatilization, (3) the effectiveness of KBo compared with potassium acetate as a VRA, and (4) the impact of KBo on dicamba volatilization with and without glufosinate. In a large-scale trial (0.4-ha plots), the effectiveness of KBo in reducing dicamba volatilization was quantified relative to a commercial dicamba application labeled for use in 2020. The addition of KBo to dicamba reduced volatility over dicamba alone and a dicamba plus potassium acetate premix. As KBo concentration increased in the dicamba spray solution, volatilization was exponentially reduced. Dicamba volatilization with the addition of KBo at 0.01 M was comparable to dicamba plus potassium acetate at 0.05 M. Potassium tetraborate tetrahydrate was more effective than potassium acetate at reducing volatility of a dicamba plus glufosinate mixture. In large-scale experiments over a 30-h period, the addition of KBo to a diglycolamine plus glyphosate mixture lowered dicamba volatilization 82% to 89% over the herbicide mixture alone. Overall, the addition of KBo to dicamba appears promising as a VRA compared with what is commercially available.

Improvement of a Pb2Ti2O5.4F1.2 Photoanode for Solar Water Splitting by Refining the Cocatalyst and Electrolyte

Abstract Photoanode materials that are active and stable for visible-light water oxidation benefit the development of artificial photosynthesis schemes, including overall water splitting and CO2/N2 fixation. The pyrochlore-structured oxyfluoride Pb2Ti2O5.4F1.2 is an n-type semiconductor with a bandgap of 2.4 eV; it has been used as a photoanode for water oxidation. In this work, cocatalysts and electrolytes that efficiently promote photoelectrochemical water oxidation on Pb2Ti2O5.4F1.2 were investigated in an aqueous solution at pH ∼9. Among the investigated transition-metal cocatalysts, cobalt phosphate (CoPi) was found to result in the best performance. The performance of the CoPi-modified Pb2Ti2O5.4F1.2 electrode toward the photoelectrochemical oxidation of water depended on the electrolyte used. As a general trend, proton-accepting electrolytes such as tetraborate, phosphate, and bicarbonate led to higher performance. The highest photoanodic current was obtained under simulated sunlight irradiation (AM1.5G, 100 mW cm−2), accompanied by O2 evolution with a Faradaic efficiency close to unity, when potassium tetraborate was used as the electrolyte.

Antibacterial activity and mode of action of potassium tetraborate tetrahydrate against soft-rot bacterial plant pathogens.

Bacterial soft rot caused by the bacteria Dickeya and Pectobacterium is a destructive disease of vegetables, as well as ornamental plants. Several management options exist to help control these pathogens. Because of the limited success of these approaches, there is a need for the development of alternative methods to reduce losses. In this study, we evaluated the effect of potassium tetraborate tetrahydrate (PTB) on the growth of six Dickeya and Pectobacterium spp. Disc diffusion assays showed that Dickeya spp. and Pectobacterium spp. differ in their sensitivity to PTB. Spontaneous PTB-resistant mutants of Pectobacterium were identified and further investigation of the mechanism of PTB resistance was conducted by full genome sequencing. Point mutations in genes cpdB and supK were found in a single Pectobacterium atrosepticum PTB-resistant mutant. Additionally, point mutations in genes prfB (synonym supK) and prmC were found in two independent Pectobacterium brasiliense PTB-resistant mutants. prfB and prmC encode peptide chain release factor 2 and its methyltransferase, respectively. We propose the disruption of translation activity due to PTB leads to Pectobacterium growth inhibition. The P. atrosepticum PTB-resistant mutant showed altered swimming motility. Disease severity was reduced for P. atrosepticum -inoculated potato stems sprayed with PTB. We discuss the potential risk of selecting for bacterial resistance to this chemical.

Proteomic analysis of the mango anthracnose pathogen Colletotrichum gloeosporioides treated with borate highlights distinct mitochondrial response mechanisms

Mango anthracnose caused by the fungal pathogen Colletotrichum gloeosporioides results in substantial economic losses in postharvest fruits. Borate is an effective fungicide against C. gloeosporioides. It appears to induce mitochondrial degradation and dysfunction in the fungal conidia, but the underlying molecular mechanism remains unclear. This study investigated the effects of borate on this pathogen by examining its mitochondrial proteins, which were extracted from C. gloeosporioides mycelium after borate treatment (5 or 10 mm potassium tetraborate for 48 and 72 h) and characterized using proteomic analysis (two‐dimensional electrophoresis, 2‐DE; and mass spectrometry, MS) and bioinformatics. In total, 600 protein spots were separated by 2‐DE, and 115 of these appeared to be differentially expressed proteins (DEPs). Among the DEPs, 58 were identified using MALDI‐TOF‐TOF MS/MS. Borate treatment was found to alter the expression levels of proteins involved in seven different metabolic pathways, mostly related to energy supply and/or biosynthesis. The effect of borate on mRNA expression levels of 17 genes involved in these metabolic pathways was confirmed by real‐time quantitative reverse transcription PCR (qRT‐PCR). The results suggest that borate might have distinct effects on mitochondrial function in C. gloeosporioides and could be used to control anthracnose in postharvest mango fruits.

In situ fabrication of nanostructured Au–Bi as high-efficient electrocatalyst for water splitting

It is essential that the development of efficient and excellent stability electrocatalysts for water oxidation, which could covert effectively renewable electricity to chemical energy. Herein, it is fabricated firstly in situ that a novel Au–Bi anode oxygen-evolving catalyst with an outstanding catalytic performance for water splitting by electrolytic deposition from dilute AuCl4− solutions in potassium tetraborate electrolyte at pH 11. The H2 generated for water splitting rate of the Au–Bi is 116.38 μmol cm−2 h−1 and the Faradaic efficiency reaches 90.43% in 0.1 M tripotassium phosphate solution. Moreover, the main active component of the Au–Bi is gold oxide (Au2O3). What is more, this work expands the research on Au-based oxygen-evolving catalysts and provides increased insight into water splitting.

Reduction of Gray Mold with Preharvest Applications of Potassium Tetraborate in Greenhouse Tomato

Potassium tetraborate tetrahydrate, K2B4O7⋅4H2O (PTB), was assessed as a preharvest spray in the greenhouse for reducing gray mold of tomato fruit caused by Botrytis cinerea. Efficacy of PTB was compared with a natural proprietary product formulated by Agrichem, which was previously shown to inhibit mycelial growth of B. cinerea in vitro and was used as a positive control for greenhouse studies. Mycelial growth and spore germination of B. cinerea were completely inhibited in vitro by PTB at 25 mM. Phytotoxicity was not observed on treated tomato plants with PTB at 25 mM. Preharvest sprays were applied at three ripening stages: turning, pink, and light red. After fruit reached the final ripening stage, all fruits were harvested and stored for 10 days at either 4 or 25°C. Disease severity was reduced compared with controls after storage at either temperature when PTB was applied at the turning stage. Preharvest applications of PTB improved the marketable quality of postharvest tomato fruit and significantly reduced the incidence of gray mold in comparison with inoculated control plants, which received no preharvest fungicidal treatment.

Antibacterial Effect of Potassium Tetraborate Tetrahydrate against Soft Rot Disease Agent Pectobacterium carotovorum in Tomato.

Soft rot caused by Pectobacterium carotovorum is one of most common bacterial diseases occurring in fruits and vegetables worldwide, yet consumer-acceptable options for post-harvest disease management are still insufficient. We evaluated the effect of potassium tetraborate tetrahydrate (B4K2O7.4H2O) (PTB) on the growth of P. carotovorum using strain BA17 as a representative of high virulence. Complete inhibition of bacterial growth was achieved by treatment with PTB at 100 mM both at pH 9.2 and after adjustment to pH 7.0. Bactericidal activity was quantified and validated by counting fluorescently labeled live and dead bacterial cells using flow cytometry, and reconfirmed using qPCR with high-affinity photoreactive DNA binding dye propidium monoazide (PMA). The results of flow cytometry, qPCR, and culturing confirmed that bacterial cells were killed following exposure to PTB at 100 mM. Bacterial cell membranes were damaged following a 5-min treatment and extrusion of cytoplasmic material from bacterial cells was observed using electronic transmission microscopy. Soft rot incidence on inoculated tomato fruit was significantly reduced by dipping infected fruits in PTB at 100 mM for 5 min and no lesions developed following a 10-min treatment. PTB does not pose a hazard to human health and is an effective alternative to other bactericides and antibiotics for controlling soft rot disease of tomato caused by P. carotovorum.

Solid–Liquid Equilibrium in the Aqueous System Containing the Borates of Potassium, Rubidium, and Magnesium at 348 K

The stable phase equilibrium and phase diagram of the quaternary system K+, Rb+, Mg2+ // borate·H2O at 348 K were determined by isothermal dissolution method. The densities, refractive indices, and solubility values of the equilibrated solution in the quaternary system were measured. The crystalloid forms of the solid phase of invariant point were identified by X-ray diffraction method. The stable phase diagram, water content diagram, and the diagrams of the densities/refractive indices versus composition were plotted based on the measured data. Results show that this quaternary system at 348 K without double salt or solid solution formed belongs to a simple eutectic type. The invariant point E saturated with three salts corresponding to potassium tetraborate tetrahydrate (K2B4O5(OH)4·2H2O), rubidium pentaborate tetrahydrate (RbB5O6(OH)4·2H2O), and hungchaoite (MgB4O5(OH)4·7H2O). The crystallization field of RbB5O6(OH)4·2H2O is the maximum, meaning that it has the smallest solubility among the coexisting salts. On the solubility curves AE (cosaturated with salts MgB4O5(OH)4·7H2O and RbB5O6(OH)4·2H2O) and BE (cosaturated with salts K2B4O5(OH)4·2H2O and RbB5O6(OH)4·2H2O), the water content decreases, the densities and refractive indices increase with the increase of J(K2B4O7); on the curve CE (cosaturated with salts MgB4O5(OH)4·7H2O and K2B4O5(OH)4·2H2O), the water content and the densities increase, the refractive indices decrease with the increase of J(K2B4O7).

Effect of Doxorubicin and Some Boron Compounds on Erythrocyte Fragility In Rats

Objective: Doxorubicin is a potent antineoplastic agent used in chemical treatment of cancer. However due to their cardiotoxic effects, its use is limited and currently subject for extensive research. Cardiomyopathy caused by doxorubicin still remains as a major concern for this potent drug. This study was aimed to contribute to this issue which needs novel preventive treatment for this side effect. Materials and Methods: Boron and boron containing molecules are subject of medical research due to their properties such as antioxidant nature. They are tested in various diseases and experimental setups. In this study Boric acid, Potassium tetraborate, Amonium biborat tetrahidrat was given to male rats at 10 mg/kg/day for 6 weeks. All study groups (n=8 each) received Doxorubicin and Boron containing compounds via intraperitoneal route. Following 6 weeks blood was withdrawn and erythrocyte osmotic fragility test was applied. Results: Erythrocyte fragility values of all doxorubicin containing groups increases and become significant at 0.5, 0.6, 0.7 and 0.8% NaCl concentrations (p<0.05). Among doxorubicin groups no significant difference was observed. Results of this study suggests that doxorubicin increases erythrocyte osmotic fragility and boron containing molecules are not sufficient to recover this untoward effect on erythrocytes at the applied dosage. Conclusion: Possible mechanisms of this result and testing in a dose dependent manner can be tested in future studies.

Stable Phase Equilibrium of Aqueous Quaternary System Li+, K+, Mg2+//Borate–H2O at 348 K

The solubility values and physicochemical properties such as densities, refractive indices, and pH values of the equilibrium solution in the quaternary system Li+, K+, Mg2+//borate–H2O at 348 K were measured by isothermal dissolution method. The phase diagram, water content diagram, and the diagrams of the physicochemical properties versus composition were constructed using the measured data. Results show that this quaternary system at 348 K is of a simple type, no double salt or solid solution formed. The stable phase diagram of this quaternary system consists of one invariant point, three univariant curves, and three crystalline phase areas. The invariant point saturated with three salts corresponding to lithium tetraborate trihydrate (Li2B4O7·3H2O), potassium tetraborate tetrahydrate (K2B4O7·4H2O), and hungchaoite (MgB4O7·9H2O). The crystallization field of MgB4O7·9H2O is the maximum, meaning that the salt magnesium borate has the smallest solubility among the coexisting salts. Comparisons between the stable phase diagrams at 288 K and 348 K show that the crystallization zones of MgB4O7·9H2O decreased at 348 K, while the crystallization zones of K2B4O7·4H2O and Li2B4O7·3H2O both enlarged at 348 K. On the univariant curve CE, the water contents decrease with the increase of J(K2B4O7); the densities and refractive indices both increase with the increase of J(K2B4O7) and reach the maximum at invariant point E.

DNA damaging and biochemical effects of potassium tetraborate.

Potassium tetraborate (PTB) is a product resulting from the controlled reaction of potassium hydroxide, water and boric acid (BA). It is used in many areas of industry such as disinfectant, detergent and treatment of contact lenses. PTB is one of the boron compounds which is most commonly used in many areas of industry although very limited information is available concerning its toxicity. Therefore, in this study, it is aimed to determine genetic and biochemical effects of PTB in human blood cell cultures (n=4). PTB was added into culture tubes at various concentrations (0-1280 µg/ml). Micronucleus (MN) and chromosomal aberration (CA) tests were performed for genotoxic damage influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative effects. The results indicated that all tested concentrations of PTB were found to be non-genotoxic. In addition, low concentrations (1.25, 2.5 and 5 µg/ml) of PTB caused increases of TAC levels. Furthermore, all concentrations of PTB were not changed the TOS levels in cultured human blood cells. Based on these results, in this study it has been reported for the first time that PTB is not genotoxic and it increases the antioxidant capacity in human peripheral blood lymphocytes.