Alginate Research Articles

Alginate-based edible coating with magnoliae essential oil/β-cyclodextrin inclusion complex for Kyoho grape preservation

To prolong the shelf life of Kyoho grapes at room temperature, an edible coating composed of sodium alginate (SA) containing an inclusion complex of Magnoliae essential oil (MEO) and β-cyclodextrin (β-CD) (SA/MEO@β-CD coating) was developed. Initially, the inclusion complex of MEO with β-CD was developed to enhance its thermal stability. MEO@β-CD was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). In addition, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) results show that β-CD can improve the thermal stability of MEO. The entrapment efficiency and loading efficiency were 82.56% and 8.8%. MEO@β-CD was added to the SA coating for preserving Kyoho grapes, while monitoring the enzyme activity, total bacterial count, and sensory characteristics of the grapes. Compared to the uncoated group, the SA/MEO@β-CD coated group exhibited a significant reduction in total color difference, decay rate, weight loss rate, enzyme activity, and total number of bacterial colonies, while also slowing the decline in hardness and total soluble solids. The effect of the SA/MEO@β-CD1 group was highly effective. Conclusively, the SA/MEO@β-CD coating preserves Kyoho grapes by reducing lipid oxidation and inhibiting microbial growth, demonstrating its potential as an advanced edible packaging solution for grape preservation.

Green self-templated synthesis of P-doped mesoporous carbon from dual sodium salts with improved average pore size for capacitive deionization

Mesoporous carbon materials hold significant potential in capacitive deionization (CDI) technology owing to expansive accessible surface area, more adaptable pore structure, excellent conductivity properties and effective surface modification and functionalization. While templating is a common method for synthesizing mesoporous carbons, the commonly used hard and soft templating approaches among them suffer from the challenges of expensive precursors and templating agents, complex procedures, secondary contaminants and difficulties in template removal. In contrast, the self-templating method not only circumvents the traditional template removal or combustion steps but also eliminates the use of organic solvents and hazardous chemicals, thus minimizing environmental impact. In this paper, we have successfully synthesized phosphorus-doped mesoporous carbon with a large average pore size (paver) using a green and convenient self-templating method employing dual sodium salts of sodium alginate (SA) and sodium phytate (SP). Compared to single SA (6.76 nm) or SP (3.74 nm) self-templated carbonization, the average pore size of carbon materials obtained from dual self-templated carbonization with a proportional mixture (12.97 nm) is significantly improved. The larger average pore size provides a greater surface area for electrode-electrolyte interactions, allowing for easier access and faster diffusion of ions into the pores, thereby enhancing the electrochemical and capacitive deionization properties of the material. The resulting material demonstrates promising electrochemical and desalination performance as evidenced by possessing a specific capacitance of 175.8 A/g and an adsorption capacity of 19.65 mg/g, underlining its potential for application in capacitive deionization utilizing mesoporous carbon materials with large average pore size.

Valorization of the performance of novel and natural sodium alginate/pectin/Portulaca oleracea L. ternary composites in the adsorption of toxic methylene blue dye from the aquatic environment

This research introduces the development of a novel, sustainable, cost-effective, and eco-friendly sodium alginate (SA)-pectin (P) ternary composite, enhanced with Portulaca oleracea L. (PO) additive, which has been thoroughly investigated for its efficacy in removing hazardous methylene blue (MB) dye from wastewater. The selectivity studies using various cationic and anionic dyes were conducted. The composite microbeads that were generated underwent characterization using FTIR-ATR, SEM, XRD, zeta potential, and pHpzc analysis. Subsequently, the most favorable parameters for adsorption, including initial pH (2–12), contact time (0–180 min), adsorbent dosage (0.01–0.20 g), and temperature (298–318 K), were identified. The effect of monovalent and divalent salt concentrations on adsorption process was evaluated. The adsorption data were utilized in several isotherm (Langmuir, Freundlich, D-R, and Temkin) and kinetic (pseudo-first-order and pseudo-second-order) models. According to the Langmuir isotherm model was calculated the adsorption capacity at 298 K is 709.22 mg/g for SA/P/PO30 composite microbeads. The process of adsorption was seen to conform to a pseudo-second-order kinetic model. The results revealed that the process was both exothermic (∆Ho=−10.42kJ/mol) and spontaneous (∆Go=−26.04kJ/molat298K). Moreover, reusability analyses demonstrated that the composite microbeads that were created may be utilized several times, even after the 5th cycle. The results indicate that the developed composite microbeads have the potential to serve as an effective and inexpensive adsorbent for eliminating cationic contaminants from a wastewater.

Ionic microsphere therapy for skin trauma

The skin is an important barrier for the body. Normal wound healing can be severely impeded by inadequate angiogenesis, excessive inflammation, and increased susceptibility to infection. Therefore, effective wound therapy should focus on accelerating the healing process and providing antimicrobial properties that are essential for wound recovery. Alginate (ALG) is a biocompatible polysaccharide substance capable of forming hydrogel dressings by cross-linking with metal ions, especially with Zn2+, Fe3+, Cu2+, and Ca2+ metal ions. The hydrogel formed by them can gradually release Zn2+, Fe3+, Cu2+, and Ca2+ during the process of wound repair, which has an important role in promoting angiogenesis and collagen deposition. Here, we prepared hydrogel microspheres (MS) based on cross-linking of different ions such as Zn-MS, Fe-MS, Cu-MS, and Ca-MS and investigated the effect of these ions on wound healing. In vitro and in vivo experiments showed that MS formed by Zn2+ enhanced antimicrobial activity, MS formed by Fe3+ and Cu2+ increased the formation of collagen fibers, and MS formed by Zn2+ and Ca2+ promoted the development of capillaries. So, the hydrogel MS formed by cross-linking ALG with these four ions are of great significance and research value in the field of skin wound application.

Three-dimensional bioprinted materials in alginate-hyaluronic acid complex based hydrogel based bio-ink as absorbents for heavy metal ions removal

Major environmental and health effects arise from the presence of heavy metals in water, including lead (Pb²⁺), mercury (Hg²⁺), chromium (Cr⁶⁺), cadmium (Cd²⁺), and arsenic (As³⁺), which contribute significantly to water pollution and pose severe risks to ecosystems and human health. This study demonstrates the fabrication of 3D printed self-regenerative functional living materials, a fungi, by using mixture of sodium alginate and hyaluronic acid hydrogel as absorbents to remove the heavy metals from the environment. Bioprinting has emerged as a transformative technology for fabricating complex biological constructs. The bioprinting experiments, conducted with optimized parameters, revealed the viscoelastic behavior of the hydrogel, suitable for 3D bioprinting applications. MicroCT analysis indicated that the hydrogel's porosity and structural properties support fungal growth in a controlled three-dimensional setting. Microscopic analyses, including phase contrast microscopy, FESEM, and HRTEM, provided insights into the cellular morphology of the bioprinted constructs. Cell viability was assessed using flow cytometry with FDA and PI staining, showing a significant population of live cells, affirming the biocompatibility of the hydrogel. Additionally, the study explored the hydrogel's potential for heavy metal removal from water samples. Multi-element standard solutions containing Copper(Cu²⁺), Cadmium(Cd²⁺), Nickel(Ni²⁺), Cobalt(Co²⁺), and Ferrous(Fe³⁺) ions at different concentrations were incubated with hydrogel patches embedded with Aspergillus flavus. Atomic absorption spectrophotometry analysis showed rapid initial removal rates of metal ions, with maximum removal efficiency achieved around the 12th hour. Comparisons with a control hydrogel without the fungal strain demonstrated significantly enhanced removal efficiency due to the presence of Aspergillus flavus. This study highlights the dual functionality of sodium alginate with hyaluronic acid -based hydrogels for bioprinting fungi and for environmental remediation of heavy metals, offering promising applications in environmental cleanup.

Boric Acid Removal from Water with Alginate Based Beads and Films as Adsorbents

High levels of boron cause harmful effects on humans, animals and plants. While boron is an important auxiliary nutrient for plants, excessive amounts cause toxic effects. The removal of boron in wastewater from chemical processes, industrial processes and agriculture is an important field of study. One of the most widely used methods for boron removal is adsorption method due to its low cost and ease of processing. In this study, adsorption method and sodium alginate microcapsules and sodium alginate-carbon nanotube film adsorbents were used as adsorbents for boric acid removal. In adsorption studies, sodium alginate-carbon nanotube film adsorbents with 42.11% boron removal were more efficient than sodium alginate microcapsules As a result of kinetic studies, it was found that the study was consistent with the pseudo-first order kinetic model.

Intelligent microneedle patch based on functionalized alginate and chitosan for long-term self-regulated insulin delivery

Severely diabetic patients need insulin input to maintain the body's glycemic balance. However, traditional injection methods are often associated with poor adherence and an increased risk of hypoglycemia. Microneedle technology offers a promising solution by minimizing pain and trauma during insulin administration. Nonetheless, achieving prolonged glycemic control by microneedle with high insulin loading remains a significant challenge. Herein, we introduce an innovative microneedle patch that draws inspiration from the elegant light-induced blooming of water lily petals. The patch features a glucose-responsive hydrogel network crafted from two modified polysaccharide polymers, which enables the delivery of long-acting insulin without depending on glucose oxidase. By incorporating phenylboronic acid-modified sodium alginate, quaternary ammonium chitosan, and polyvinyl alcohol into a hydrogel matrix, we have created a microneedle system that harbors dynamic borate ester linkages and electrostatic attractions, resulting in heightened sensitivity to blood glucose levels. The electrostatic interaction acts as a relatively stable crosslinking point, balancing the dynamic reproducibility response based on the borate ester bond. This self-adaptive hydrogel can regulate insulin-controlled release by responding to changes in glucose concentration. Herein, we achieved massive insulin loading (20 IU) with long lasting glycaemic control (48 h) in a single treatment of diabetic SD rats.

Gelation-pyrolysis strategy for fabrication of advanced carbon/sulfur cathodes for lithium-sulfur batteries

The development of high-performance carbon-based composite hosts play decisive roles in the electrochemistry of lithium sulfur batteries. Herein, a novel metal-ion induced gelation self-assembly technology is reported to construct sodium alginate carbon (SAC) based polar hierarchical carbon composites with cross-linked network architecture and in-situ co-grown cross-linked polar nanoparticles. Interestingly, it shows high versatility to an extensive array of materials including metals, alloys, and metallic oxides. As a representative, NiCo alloy nanoparticles are chosen to obtain the SAC/NiCo composite host for sulfur in LSBs, which possess superior physical/chemical adsorption capabilities and catalytic conversion kinetics to polysulfide in virtue of synergistic interaction between the hierarchical pore structures and NiCo catalyst. The designed SAC/NiCo-S cathode shows superior electrochemical performance with excellent rate capacity (2 C: 693.5 mAh/g) and enhanced cycling stability (764.3 mAh/g at 0.1 C after 240 cycles). This work provides a straightforward approach for fabricating multifunctional carbon composites with adjustable component for advanced energy storage system.

Extraction and Characterization of Cellulose from Kelp (Laminaria japonica) Residue

Abstract Kelp residue is the byproduct of industrial production of sodium alginate from kelp. An in-depth utilization of kelp residue not only creates higher economic value but also opens up a new pathway for comprehensive utilization of kelp. In this study, a method of first removing acid-soluble ash and then depleting protein was employed to extract cellulose components from kelp residue. Single-factor experiments demonstrated that treating kelp residue with 1.2 mol/L hydrochloric acid at a solid-liquid ratio of 1:5 effectively removed acid-soluble ash. Subsequently, further treatment with 3% NaOH at a solid-liquid ratio of 1:10, conducted at 100 °C for 140 minutes, reduced the protein content in the extract to below 1%, with a cellulose yield of 33.5%. Fourier transform infrared spectroscopy analysis revealed that characteristic peaks attributed to cellulose existed in the extractive, while electron microscopy observations indicated that the extracted kelp residue cellulose exhibited irregular thin-sheet structures. The cellulose obtained in this study lays the foundation for its further utilization and processing into cellulose-derived products.

Mesenchymal stem cells-laden self-healing hydrogel microfiber fragments from microfluidic bubbles interrupted spinning for wound healing

Mesenchymal stem cells (MSCs) loaded hydrogels have shown positive therapeutic effects in skin wound treatment, while their application is still impractical on the wound bed. Here, we propose novel MSCs-loaded self-healing hydrogel microfiber fragments from microfluidic bubbles interrupted spinning for wound healing. The hydrogel microfibers are composed of sodium alginate, gelatin, polyvinyl alcohol, and borax, vesting them with good biocompatibility and self-healing ability. Their fragments with adjustable length are generated by introducing bubbles into microfluidic channels. We have demonstrated that the self-healing microfibers can promote the proliferation of MSCs, which are seeded alongside fragments’ surfaces to further facilitate the wound healing process. In addition, these MSCs laden self-healing hydrogel microfibers (MSG-fibers) showed obvious healing effect in rat wound model, which significantly promoted granulation tissue formation, collagen deposition and vascular construction at the wound site. Thus, we believe that our proposed MSC-laden microfiber fragments are valuable in promoting wound healing and have promising prospect in becoming new options for wound management.

Injectable self-healing alginate/PEG hydrogels cross-linked via thiol-Michael addition bonds for hemostasis and wound healing

In this study, an alginate/PEG hydrogel was developed via a thiol-Michael addition reaction between oxidized quinone of catechols on dopamine-grafted sodium alginate (SA-DA) and sulfhydryl groups of 4-arm polyethylene glycol tetra-thiol (4-arm PEG-SH) under mildly basic conditions. Through the formation of thiol-terminated catechol groups, the accompanying oxidized catechols are reduced, significantly strengthening the internal network structure of the hydrogel and improving tissue adhesion. Meanwhile, the hydrogels have excellent self-healing properties due to the dynamic non-covalent bonds between the groups. Adjustment of hydrogel properties by varying the mass ratio of two hydrogel precursors. Due to the high content of thiol-terminated catechol groups, the Gel 3 exhibited good tissue adhesion, rapid self-healing ability, and other multifunctions beneficial to wound healing, including killing of E. coli and S. aureus, rapid hemostasis and promoting migration of L929 cells. The full-thickness skin wound model shows that the hydrogel dressing significantly accelerated wound contraction, with increased granulation tissue thickness, collagen disposition, and enhanced vascularization, thus promoting wound healing. Therefore, the thiol-Michael addition reaction is an effective method for creating multifunctional hydrogels, and the injectable self-healing alginate/PEG hydrogels prepared in this way could be used in the biomedical area as wound healing dressing materials.

Microencapsulation of Azadirachta indica oil and Tinospora cordifolia extracts blend for single-use applications

The innovative utilization of the microencapsulation technique and the antimicrobial properties of Azadirachta Indica (A.I.) and Tinospora cordifolia (T.C.) present a novel approach to producing antiseptic wipes. In this study, microcapsules with A.I. oil and T.C. extracts were synthesized and integrated into non-woven cotton fabric aiming to create disposable antiseptic medical wipes. The T.C. extract and A.I. oil were used in a 1:1 ratio and microencapsulated utilizing sodium alginate as the encapsulation material through an ionic gelation process. The optical microscope analysis of microcapsules revealed diverse microcapsule sizes, ranging from 50 μm to 180 μm in diameter and scanning electron microscopy revealed that the treated fabric has uniform distribution of microcapsules across the entire fabric surface. The antimicrobial efficacy was evaluated through disk diffusion for the extract and oil and the fabric's antimicrobial activity was assessed by measuring bacterial reduction. The treated fabric displayed substantial reductions in both Gram-positive bacteria (Staphylococcus aureus (87 %) and Bacillus cereus (99 %)) and Gram-negative bacteria (Escherichia coli (81 %) and Pseudomonas aeruginosa (99 %)). Although the treated fabric exhibited a slight decrease in whiteness (48.5–39), this alteration did not adversely affect other fabric properties. These findings underscore the potential of microencapsulated natural extracts in producing effective antimicrobial medical wipes, thereby contributing to infection control.

S1522 Efficacy and Safety of Sodium Alginate as an Injection Solution in Endoscopic Submucosal Dissection: A Systematic Review and Meta-Analysis

S1522 Efficacy and Safety of Sodium Alginate as an Injection Solution in Endoscopic Submucosal Dissection: A Systematic Review and Meta-Analysis

3D morphometry of endothelial cells angiogenesis in an extracellular matrix composite hydrogel

Human umbilical vein endothelial cells (HUVECs) play a fundamental role in angiogenesis. Herein, we introduce digital holographic microscopy (DHM) for the 3D quantitative morphological analysis of HUVECs in extracellular matrix (ECM)-based biomaterials as an angiogenesis model. The combination of volumetric information from DHM and the physicochemical and cytobiocompatibility data provided by fluorescence microscopy and cytology offers a comprehensive understanding of the angiogenesis-related parameters of HUVECs within the ECM. DHM enables label-free, non-contact, and non-invasive 3D monitoring of living samples in real time, in a quantitative manner. In this study, the human amniotic membrane (HAM) is decellularized, pulverized, and combined with sodium alginate hydrogel to provide an in vitro substrate for modeling HUVEC angiogenesis. Our results demonstrate that modifying alginate hydrogel with HAM enhances its biofunctionality due to the presence of ECM components. Moreover, the DHM results reveal an increase in its porous properties, which, in turn, aids in interpreting the tubulation results.

Encapsulating Rhodamine 6G in Oxidized Sodium Alginate Polymeric Hydrogel for Photodynamically Inactivating Cancer Cells.

As cancer therapy progresses, challenges remain due to the inherent drawbacks of conventional treatments such as chemotherapy, gene therapy, radiation therapy, and surgical removal. Moreover, due to their associated side effects, conventional treatments affect both cancerous and normal cells, making photodynamic therapy (PDT) an attractive alternative. As a result of its minimal toxicity, exceptional specificity, and non-invasive characteristics, PDT represents an innovative and highly promising cancer treatment strategy using photosensitizers (PSs) and precise wavelength excitation light to introduce reactive oxygen species (ROS) in the vicinity of cancer cells. Poor aqueous solubility and decreased sensitivity of Rhodamine 6G (R6G) prevent its use as a photosensitizer in PDT, necessitating the development of oxidized sodium alginate (OSA) hydrogelated nanocarriers to enhance its bioavailability, targeted distribution, and ROS-quantum yield. The ROS quantum yield increased from 0.30 in an aqueous environment to 0.51 when using alginate-based formulations, and it was further enhanced to 0.81 in the case of OSA. Furthermore, the nanoformulations produced fluorescent signals suitable for use as cellular imaging agents, demonstrating contrast-enhancing capabilities in medical imaging and showing minimal toxicity.

Field-dependent nonlinear electrical response characteristics in polymer dielectrics with sodium alginate scaffold

Field-dependent nonlinear electrical response characteristics in polymer dielectrics with sodium alginate scaffold

Modeling conformational changes in alginic acid oligomers induced by external forces

In this study, the mechanism and nature of mechanical force-induced conformational transitions of alginate oligomers with different ratios of β-D-mannuronic acid (M unit) and α-L-guluronic acid (G unit) units were investigated. The influence of the type of glycosidic linkage in either homo- or heterooligomers on the nature of conformational transitions was also considered. For this purpose, two different theoretical methods were used: quantum mechanics (QM) at the DFT level with the EGO (Enforced Geometry Optimization) approach previously tested also for other saccharide systems, and molecular dynamics (MD) simulations within hybrid interaction potentials, which take into account both the ab initio (QM) level of theory and classical molecular mechanics (MM) force fields. This allowed to characterize in detail the structural and energetic properties of the conformational transition occurring upon the influence of external, mechanical forces (e.g. ring conformations at the path of ring-inversion process as well as the energies corresponding to initial, final and intermediate states). The results indicate qualitatively various responses against the applied force, depending on the G:M ratio, which have their source in differing topologies of glycosidic linkage in either G or M units. This is of potential relevance for determining the content of naturally heterogeneous alginate chains by the AFM experimental studies. The effects of explicit solvent and non-zero temperature are not of primarily importance in the context of determined stretching properties.

Binary Biomass-Based Electrolyte Films for High-Performance All-Solid-State Supercapacitor.

Solid-state electrolytes have received widespread attention for solving the problem of the leakage of liquid electrolytes and effectively improving the overall performance of supercapacitors. However, the electrochemical performance and environmental friendliness of solid-state electrolytes still need to be further improved. Here, a binary biomass-based solid electrolyte film (LSE) was successfully synthesized through the incorporation of lignin nanoparticles (LNPs) with sodium alginate (SA). The impact of the mass ratio of SA to LNPs on the microstructure, porosity, electrolyte absorption capacity, ionic conductivity, and electrochemical properties of the LSE was thoroughly investigated. The results indicated that as the proportion of SA increased from 5% to 15% of LNPs, the pore structure of the LSE became increasingly uniform and abundant. Consequently, enhancements were observed in porosity, liquid absorption capacity, ionic conductivity, and overall electrochemical performance. Notably, at an SA amount of 15% of LNPs, the ionic conductivity of the resultant LSE-15 was recorded at 14.10 mS cm-1, with the porosity and liquid absorption capacity reaching 58.4% and 308%, respectively. LSE-15 was employed as a solid electrolyte, while LNP-based carbon aerogel (LCA) served as the two electrodes in the construction of a symmetric all-solid-state supercapacitor (SSC). The SSC device demonstrated exceptional electrochemical storage capacity, achieving a specific capacitance of 197 F g-1 at 0.5 A g-1, along with a maximum energy and power density of 27.33 W h kg-1 and 4998 W kg-1, respectively. Furthermore, the SSC device exhibited highly stable electrochemical performance under extreme conditions, including compression, bending, and both series and parallel connections. Therefore, the development and application of binary biomass-based solid electrolyte films in supercapacitors represent a promising strategy for harnessing high-value biomass resources in the field of energy storage.

Preparation and Properties of Thermoregulated Seaweed Fibers Based on Magnetic Paraffin wax@calcium Carbonate Microcapsules

In order to enhance the application of thermoregulated materials, magnetic phase change microcapsules were prepared using a self-assembly method. Paraffin wax was chosen for its fine thermoregulation properties as the core material, while Fe3O4 nanoparticles doped in calcium carbonate served as the hybrid shell material. The microcapsules were then blended with sodium alginate and processed into seaweed fibers through wet spinning. The microstructure, thermal, and magnetic properties of the microcapsules were analyzed using scanning electron microscopy, energy dispersive X-ray spectroscopy, a laser particle size analyzer, an X-ray diffractometer, a differential scanning calorimeter, a thermogravimetric analyzer, and a vibrating sample magnetometer. The thermoregulation of the fibers was evaluated using a thermal infrared imager. The results indicated that the microcapsules had a uniform size distribution and good thermal properties. When the mass fraction of Fe3O4 nanoparticles was 8%, the microcapsules exhibited a saturation magnetization of 2.44 emu/g and an enthalpy value of 94.25 J/g, indicating effective phase change and magnetic properties. Furthermore, the thermoregulated seaweed fibers showed a high enthalpy value of 19.8 J/g with fine shape, offering potential for developing multifunctional fiber products.

Sodium alginate edible films incorporating cactus pear extract: antimicrobial, chemical, and mechanical properties

The potential benefits of biodegradable and functional food packaging materials have garnered increasing attention in recent years. Sodium alginate (SA), a commonly utilized polysaccharide, is particularly noteworthy for producing biodegradable films for food packaging. This study aimed to investigate SA-based edible films enriched with various proportions of cactus pear peel extract (CPPE). We assessed the films and extracts for total phenolic content, antimicrobial and antioxidant activities, as well as mechanical properties. Cactus pear peels powder (CPPP) exhibited higher contents of total polyphenols (1243.82 mg GAE/100 g), total flavonoids (18.92 mg QE/100 g), and betalains (2.28 mg/100 g). The main constituents detected were catechol, pyrogallol, catechin, and alpha-coumaric acid, with concentrations of 1013.82, 223.45, 148.21, and 101.02 ppm, respectively, contributing about 45.71%, 9.85%, 6.54%, and 4.46% of the total phenolic compounds. The thickness of the SA films increased from 0.220 mm to 0.265 mm. The tensile strength values ranged from 1.98 to 3.12, while the elongation at break values for SA-CPPE films decreased relative to the plain SA film. Moreover, the inclusion of CPPE improved the barrier characteristics (with water vapor permeability values for SA films with 1%, 2%, and 3% CPPE ranging from 0.72 × 10−5 g·h−1·m−1·Pa−1 to 1.68 × 10−5 g·h−1·m−1·Pa−1) and induced variations in flexibility and resistance. The plain SA film did not exhibit any inhibitory activity against bacteria and fungi. However, the inclusion of CPPE in alginate films showed favorable antibacterial properties, which improved progressively with increasing CPPE concentration. These findings highlight the potential of incorporating active alginate-based films in food preservation.