You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology II1 Apr 2016MP85-07 URINARY MIRNAS AS A PREDICTOR OF TUMOR RECURRENCE IN PATIENTS WITH CLEAR CELL RENAL CELL CARCINOMA Rodolfo Borges Dos Reis, Xian Shu, Yuanqing Ye, Beatriz Sanchez Espiridion, Maosheng Huang, Mehrad Adibi, Leonardo Borregales, Lisly Chery, Jose Karam, Xifeng Wu, and Christopher Wood Rodolfo Borges Dos ReisRodolfo Borges Dos Reis More articles by this author , Xian ShuXian Shu More articles by this author , Yuanqing YeYuanqing Ye More articles by this author , Beatriz Sanchez EspiridionBeatriz Sanchez Espiridion More articles by this author , Maosheng HuangMaosheng Huang More articles by this author , Mehrad AdibiMehrad Adibi More articles by this author , Leonardo BorregalesLeonardo Borregales More articles by this author , Lisly CheryLisly Chery More articles by this author , Jose KaramJose Karam More articles by this author , Xifeng WuXifeng Wu More articles by this author , and Christopher WoodChristopher Wood More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2273AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Up to 25% of patients with Renal Cell Carcinoma (RCC) present at advanced stages and nearly 40% of patients who undergo curative surgery experience recurrence. Micro RNAs (miRNA) are non-coding strands of RNA involved in gene expression. The relative stability of microRNA in serum and urine and its ability to accurately classify specific disease states through expression profiles have turned microRNA quantification as a promising biomarker. We aim to identify miRNAs with altered expression in the urine of patients with clear cell RCC (ccRCC), prior to and after curative surgery and determine predictors of prognosis. METHODS Pre and postoperative urine samples of 117 patients with ccRCC were prospectively collected. Relevant clinicopathological data were abstracted from medical records. Total RNA and miRNA were extracted from urine samples. A set of 137 miRNAs was evaluated using high-throughput BioMark HD Real-Time PCR system. Relative quantifications (RQ) of miRNAs levels in pre and postoperative urine samples were calculated and compared using Wilcoxon rank test. To measure the change of miRNA levels from pre- to postoperative, we calculated the ratio of post/pre RQs. The association between clinical variables and metastasis were tested using log-rank test. RESULTS Over half of the patients presented with pathological stage 3 (pT3) or Fuhrman grade (FG) III-IV (69%). During a mean 73 months follow-up period, 22 patients recurred and 11 succumbed to cancer. 34 postoperative miRNAs were significantly altered (p<0.05) compared to their preoperative levels. After adjusting for age, gender, BMI, race, stage, FG and surgery type, five miRNAs (miR-191-5p, miR-324-3p, miR-186-5p, miR-93-5p and miR-30b-5p) were significantly reduced in postoperative samples. The 50% or more reduced postoperative levels of these urine miRNAs conferred 2 to 4-fold increased risk of metastasis with miR-191-5p being most significant (HR=4.16, 95% CI=1.38-12.58, p=0.011). In pT3 patients the reduction in the expression of miR-191-5p, miRNA-324-3p and miRNA-186-5p was associated with an increased risk of developing metastases (HR=7.43, 95% CI=1.81-30.5, p=0.005; HR=12.8, 95% CI= 2.53-64.7, p=0.002; HR=4.8, 95% CI=1.26-18.2, p=0.02 respectively). CONCLUSIONS Urine miRNAs are promising biomarkers. We demonstrated that miR-191-5p, miR-324-3p, miR-186-5p are potential molecular markers to predict recurrence in pT3 patients when adjusted to other variables. This can impact our understanding of this heterogeneous disease and the associate treatments. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1100 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Rodolfo Borges Dos Reis More articles by this author Xian Shu More articles by this author Yuanqing Ye More articles by this author Beatriz Sanchez Espiridion More articles by this author Maosheng Huang More articles by this author Mehrad Adibi More articles by this author Leonardo Borregales More articles by this author Lisly Chery More articles by this author Jose Karam More articles by this author Xifeng Wu More articles by this author Christopher Wood More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...