Post-mining Soil Research Articles

Growth and physiology responses of Samanea saman inoculated with arbuscular mycorrhizal fungi in silica post-mining soil media using biodegradable pots

Arbuscular mycorrhizal fungi (AMF) and biodegradable pots are environmentally friendly and enhance plant growth in adverse soil conditions. These studies explored AMF interactions and biodegradable pots in physiology, growth, and the uptake of nutrients in Samanea saman seedlings. The present research interactive effects of biodegradable pots with different compositions and raw material sizes with and without AMF inoculation on S. saman grown in silica post-mining soil media. Results indicated AMF inoculations significantly improved leaf chlorophyll content, photosynthetic rate, heights, diameters, biomass, as well as nutrient absorption of S. saman as compared with non-inoculated plants in biodegradable pots. AMF and biodegradable pots composed of 15% used newspaper, 70% compost, 5% cocopeat, and 10% rock phosphate showed the best results and increased the leaf chlorophyll content, photosynthesis rate, height, diameter, and total biomass of the plants by 161.1%, 208.7%, 263.6%, 118.1%, and 269.9%, respectively, compared to biodegradable pots composed only of used newspaper. Additionally, uptake of the nutrients nitrogen, phosphate, potassium, and magnesium increased by 365%, 800%, 369%, and 250%, respectively. By the fourteenth week after transplanting, the C/N ratio of the organic pot decreased significantly. Thus, AMF and biodegradable pots containing compost and rock phosphate interact positively and enhance the growth of S. saman under adverse soil conditions. These findings suggest that biodegradable pots containing compost and rock phosphate show potential as more environmentally friendly replacements for plastic bags (polybags) as growth medium containers for seedling production.

Establishment of Nitrogen-Fixing Frankia, Arbuscular Mycorrhizal Fungi, and Their Effects on Alder (Alnus glutinosa L.) Growth in Post-Mining Heap Soils

Planting nitrogen-fixing plants in post-mining sites and similar degraded areas is a common approach to speed up soil development and buildup of the nitrogen pool in soil organic matter. The aim of this study was to explore if slower growth of alder seedlings in initial post-mining sites results from adverse soil conditions or lack of microbial symbionts. To address this question, we sampled young soil (age 15 years) and more developed soil (age 70 years) from heaps after coal mining near Sokolov (Czech Republic). Soil samples were sterilized and not inoculated or inoculated with arbuscular mycorrhizal fungi (AMF) or AMF + Frankia, followed by planting with alder (Alnus glutinosa) seedlings germinated and precultured under sterile conditions. The effect of soil age on alder growth appeared to be non-significant. The only significant growth effect was seen with Frankia inoculation, implicating this inoculum as a key factor in later succession in post-mining soils. When the soil was fully inoculated, alder biomass was higher in developed soil supplied with iron (Fe) and phosphorus (P), indicating that iron and phosphorus availability may affect alder growth. In young soil, alder growth was highest with a combination of iron, phosphorus, and sulfur (S), and a positive effect of sulfur in young soil may correspond with a reduced, alkaline soil pH and increased phosphorus and iron availability.

Soil amendments influence early plant survival and growth in reclamation of severely degraded lands by gold mining in the Peruvian Amazon

Gold mining has been causing the most severe impacts on the soils of the Peruvian Amazon. It has created challenges for their recovery. In this context, soil amendments could play a crucial role in plant establishment in post-mining soils. The study aimed to analyze the effects of two amendments on the early plant survival and growth of seven species in the reclamation of severely degraded lands by gold mining in the Southeastern Peruvian Amazon. The study was based on a completely randomized block design, including 2-amendment treatments (T1: sawdust + island guano manure and T2: T1 + organic soil + hydrogel) and a control. The plant survivorship, height growth, diameter growth, and biomass accumulation were measured. This study found that amendments may be effective at increasing survivorship and plant growth in degraded lands by gold mining in the Peruvian Amazon. The amendments increased the survival, diameter, height, and biomass of most plant species in the study. In general, survivorship and plant growth in T2 were high compared to T1. At the end of the experiment, the highest survivorship was for an Indigofera suffruticosa and Crotalaria pallida (>80%). The diameter growth was higher in T2 than in T1. The species growing fastest in diameter (>1.5 cm) were Crotalaria cajanifolia, C. pallida and Ochroma pyramidale. Soil amendments provided similar effects on height for most species except for I. suffruticosa. Therefore, C. pallida, I. suffruticosa, C. cajanifolia and O. pyramidale are key species to be considered in reforestation and/or restoration initiatives, due to its potential to acclimate and establish itself in severely degraded areas.

A second life for mining waste as an amendment for soil remediation

Mining is a cornerstone of the productive system, particularly in sectors, such as renewable energy and electronic equipment production, where its significance is anticipated to grow in the coming years. Aligned with the principles of Nature-Based Solutions and Zero Waste policies, recycling mining waste as soil amendments could concurrently restore degraded areas and reduce the disposal of mining waste. In this context, we aimed to remediate a post-mining soil primarily impacted by heavy metal pollution using an alkaline waste generated in dunite exploitation, either in combination with compost or independently in field conditions. The objectives were to minimize heavy metal(loid)s mobility (As, Cu, Cd, Ni, Pb, and Se), to improve soil health, and stimulate plant growth (phytoremediation, Lolium perenne L. was used). Results revealed that the combination of dunite and compost successfully reduced the concentrations of available Cu and Ni in the soil by more than four times, significantly enhanced soil properties, and promoted the harvest of a greater biomass. Additionally, Lolium perenne L. demonstrated phytostabilizing capacity for Cu and Ni in the soil treated with the amendments. In conclusion, the utilization of combined dunite-based and organic amendments proves to be a favorable strategy for restoring polluted post-mining soils.

Biochar-nanoparticle combinations enhance the biogeochemical recovery of a post-mining soil

Here we addressed the capacity of distinct amendments to reduce arsenic (As), copper (Cu), selenium (Se) and zinc (Zn) associated risks and improve the biogeochemical functions of post-mining soil. To this, we examined nanoparticles (NPs) and/or biochar effects, combined with phytostabilization using Lolium perenne L. Soil samples were taken in a former metal mine surroundings. Ryegrass seeds were sown in pots containing different combinations of NPs (zero-valent iron (nZVI) or hydroxyapatite (nH)) (0 and 2 %), and biochar (0, 3 and 5 %). Plants were grown for 45 days and the plant yield and element accumulation were evaluated, also soil properties (element distribution within the soil fractions, fertility, and enzymatic activities associated with microbiota functionality and nutrient cycling) were determined. Results showed biochar-treated soil had a higher pH, and much higher organic carbon (C) content than control soil and NP-treated soils, and it revealed increased labile C, total N, and available P concentrations. Soil treatment with NP-biochar combinations increased exchangeable non-acid cation concentrations and reduced exchangeable Na%, improved soil fertility, reduced sodicity risk, and increased ryegrass biomass. Enzymatic activities, particularly dehydrogenase and glucosidase, increased upon the addition of biochar, and this effect was fostered by NPs. Most treatments led to a significant reduction of metal(loid)s contents in biomass, mitigating contamination risks. The two different NPs had similar effects in many parameters, nH outperformed nZVI in terms of increased nutrients, C content, and enzymatic activities. On the basis of our results, combined biochar-NP amendments use, specially nH, emerges as a potential post-mining soil restoration strategy.

Assessment of carbon sequestration potential of mining areas under ecological restoration in China

Mining activities aggravate the ecological degradation and emission of greenhouse gases throughout the world, thereby affecting the global climate and posing a serious threat to the ecological safety. Vegetation restoration is considered to be an effective and sustainable strategy to improve the post-mining soil quality and functions. However, we still have a limited knowledge of the impact of vegetation restoration on carbon sequestration potential in mining areas. In this pursuit, the present study was envisaged to integrate the findings from studies on soil organic carbon (SOC) sequestration in mining areas under vegetation restoration with field monitoring data. The carbon sequestration potential under vegetation restoration in China's mining areas was estimated by using a machine learning model. The results showed that (1) Vegetation restoration exhibited a consistently positive impact on the changes in the SOC reserves. The carbon sequestration potential was the highest in mixed forests, followed by broad-leaved forests, coniferous forests, grassland, shrubland, and farmland; (2) The number of years of vegetation restoration and mean annual precipitation were found to be the important moderating variables affecting the SOC reserves in reclaimed soils in mining areas; (3) There were significant differences in the SOC sequestration potential under different vegetation restoration scenarios in mining areas in China. The SOC sequestration potential reached up to 9.86 million t C a−1, when the soil was restored to the initial state. Based on the meta-analysis, the maximal attainable SOC sequestration potential was found to be 4.26 million t C a−1. The SOC sequestration potential reached the highest level of 12.86 million t C a−1, when the optimal vegetation type in a given climate was restored. The results indicated the importance of vegetation restoration for improving the soil sequestration potential in mining areas. The time lag in carbon sequestration potential for different vegetation types in mining areas was also revealed. Our findings can assist the development of ecological restoration regimens in mining areas to mitigate the global climate change.

Native plant composition and soil microfauna in limestone post-mining land as potential for development of ruminant forage

Abstract. Harwanto, Karti PDMH, Suwardi, Abdullah L. 2023. Native plant composition and soil microfauna in limestone post-mining land as potential for development of ruminant forage. Biodiversitas 24: 6332-6342. Limestone post-mining land is a potential marginal land, through revegetation and integration, as a forage provider. Therefore, utilizing an exploratory systematic random sampling, this research explored the native botanical composition that had the potential for forage and soil microfauna associated with plants in the limestone post-mining land of PT Sinar Tambang Arthalestari, Banyumas, Central Java, Indonesia from June to September 2023. The results showed that the limestone post-mining soil had a slightly alkaline pH and low fertility. The botanical composition comprised 16 grasses, four legumes, and 21 forbs. Native plants that have the highest Importance Value Index (IVI) were grasses; Ischaemum rugosum, Imperata cylindrica, Paspalum scrobiculatum, and legumes; Mimosa pudica, Centrosema pubescens, and Calopogonium mucunoides. Grasses contained 8.35-12.61% Crude Protein (CP) and 42.50-50.78% Total Digestible Nutrient (TDN), while legumes contained 17.06-17.74% CP, 62.12-64.75% TDN. Mycorrhizal colonization and populations of Azotobacter, Azospirilum, and Rhizobium were associated with native plants in the rhizosphere. Conclusively, the grasses I. rugosum, I. cylindrica, P. scrobiculatum, and the legumes M. pudica, C. pubescens, and C. mucunoides have the potential to forage on limestone post-mining land and are associated with soil microfauna. However, soil fertility requires further improvement by applying organic matter, nitrogen, and phosphorus fertilizers.

Optimizing Nutrient and Quality of Second Ratoon Odot Grass through Biochar Application on Post-Nickel Soil

This study aims to determine the application of various levels of biochar in post-nickel mining soil on the productivity and quality of second ratoon odot grass (Pennisetum purpureum cv. mott). This study used a completely randomized design (CRD) with 4 treatments and 4 replications. The treatments consisted of P0: without biochar (control), P1: 75 gr/polybag biochar, P2: 150 gr/polybag biochar, and P3: 225 gr/polybag biochar. Each treatment contained 30 kg/polybag of post-nickel mining soil and was given 150 g/polybag of goat manure. The data obtained were analyzed using analysis of variance and further tested with the Duncan Multiple Range Test on treatments that had a significant effect. Parameters observed included the content of dry matter (DM), organic matter (OM), crude fiber (CF), and crude protein (CP). The results showed that the application of biochar on post-mining soil had no significant effect on DM, OM, and CP content but significantly (P<0.05) different CF content of odot grass. P1 showed 9.06% DM, 84.70% OM, 21.60% CF, and 9.74% CP. This study concludes that the application of 75 g/polybag biochar on post-nickel soil showed the best quality of the second ratoon odot grass in this study.

Soil properties change, and arbuscular mycorrhizal fungi associated with plants growing on the post-gold mining land of Bombana, Indonesia

<p>This study aimed to investigate the effect of gold mining on soil properties. Soil samples were taken from the post-gold mining land, the property of PT Panca Logam Nusantara and PT Alam Buana Indonesia, and a nearby natural forest in Bombana, Southeast Sulawesi Province. The next step focused on specifying soil pH, total nitrogen (TN) and carbon (TC) concentration, C/N ratio, available phosphorus (P) concentration, cation exchange capacity (CEC), and exchangeable K, Na, Mg, Ca, Fe, Mn, Cd and Pb concentration, texture and spore amount, AMF resource and AMF colonization. The result shows that the pH in post-gold mining soil was higher than that in natural forest soil. Meanwhile, TN, TC, available P, and CEC of post-gold mining soil got lower compared with these of natural forest soil. The texture in the post-mining soil was clay loam, while that in natural forest soil was clay. Total of 10 AMF species belonging to five genera and three families were found in a post-gold mining area. Soil pH, CEC, soil texture, Mn, and total Fe had a negative relation with AMF colonization and spore count, while organic C, total N, C/N ratio, P<sub>2</sub>O<sub>5</sub> and silt had a positive relation. Sand was proven to have a strong and positive correlation with the amount of AMF species. Adding organic matter and fertilization as well as applying mycorrhizal biofertilizers, were urgently required to support the effort in restoring post-gold mining soil.</p>

Effects of vegetation restoration on the concentrations of multiple metal elements in post-mining soils

Vegetation restoration is vital for soil ecological restoration in post-mining areas, but a global-scale quantitative assessment of its effects on soil metal elements is lacking. Here, we conducted a meta-analysis with 2308 paired observations collected from 137 publications to evaluate vegetation restoration effects on the concentrations of 17 metal elements, namely K, AK (available K), Ca, Na, Mg, Fe, Mn, Zn, Cu, Al, Cr, Co, Ni, Cd, Sb, Hg, and Pb in post-mining soils. We found that (1) vegetation restoration significantly increased the concentrations of K, AK, Ca, Mg and Co by 43.2, 42.5, 53.4, 53.7, and 137.2%, respectively, but did not affect the concentrations of Na, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, Sb, Hg, and Pb; (2) the effects of vegetation restoration on soil metal concentration were seldom impacted by vegetation type, while soil depth only affected the responses of AK, Cd, and Pb concentrations to vegetation restoration, and leaf type only impacted the responses of Ca and Ni concentrations to vegetation restoration; (3) latitude, elevation, restoration year, climate, and initial soil properties were also important moderator variables of vegetation restoration effects, but their impacts varied among different metals. Overall, our results clearly showed that vegetation restoration in posting-mining areas generally have a positive effect on the concentrations of nutrient elements but did not influence that of toxic elements, which provides useful information for the restoration and reconstruction of soil ecosystem in post-mining areas.

Which Soil Properties Determine Tree Nutrient Supply in Extreme Technosol Conditions?

In recent years, there has been an increased interest in the selection of tree species for their adaptation to difficult habitat conditions in post-mining areas. As global climate change can cause changes in the circulation of elements, it is vital to understand how soil and habitat conditions affect the mineral nutritional status of trees.The study compared the nitrogen (N) and phosphorus (P) content in the leaves of various tree species on different substrates created by mining activities. The research was carried out in stands growing on reclaimed post-mining soils composed of various parent materials, including sands, clays, and ashes. The factors influencing the N and P supply to trees in the designated parent material were investigated. The soil’s physicochemical properties were determined, and biochemical analyses were conducted. The tests performed showed that there were no differences in the N and P content in the leaves of scots pine (Pinus sylvestris), black locust (Robinia pseudoacacia), black alder (Alnus glutinosa), and common birch (Larix decidua) growing on different substrates. The results confirmed a significant relationship between the mineral nutritional status of trees and the chemical (N, Mg2+, and K+) and biological (AcdPhoSP) properties of soils. The N supply was related to the contents of nitrogen total (Ntot) and exchangeable magnesium (Mg2+) in soils and to the specific activity of acid phosphatase (AcdPhoSP). The level of phosphorus (P) supply to the studied tree species growing on technogenic soils was shown to be a factor related to the activity of acid phosphatase (AcdPhoSP).

Carbon Sequestration in Remediated Post-Mining Soils: A New Indicator for the Vertical Soil Organic Carbon Variability Evaluation in Remediated Post-Mining Soils

The present study experimentally investigated two different open-cast post-mining areas with different remediation methods for the vertical distribution of sequestered soil organic carbon (SOC). The study has been performed for two soil layers (0–15 cm, and 15–30 cm) for the four areas with different remediation advancement (up to 20 years) at both studied post-mining soils: the limestone post-mining soil remediated with embankment and lignite post-mining soil remediated with sewage sludge. The study revealed that SOC is more stable within soil depths for lignite post-mining soil remediated with sewage sludge in comparison to the limestone post-mining soil remediated with embankment. The lignite post-mining soil remediated with sewage sludge showed a better hydrophobicity, humidity, aromaticity, and C/N ratio according to the 13C NMR. Therefore, in that soil, an increased microbial community has been observed. The study observed a positive correlation between GRSP content with a fungi community within soil depths. For lignite post-mining soil remediated with sewage sludge, the activity of ureases and dehydrogenases was generally lower compared to the post-mining soil remediation with embankment. The investigation found good parameters of Ce and NCER which for both studied areas were negative which indicate for the privilege of the higher capturing of CO2 over its release from the soil into the atmosphere. The study finds no relevant changes in SOC, POXC, TC, and LOI content within soil depth and remediation age. Due to the lack of a possible well-describing indicator of the vertical distribution of SOC stability in post-mining remediation soil, we proposed two different indicators for differentially managed post-mining soil remediations. The model of calculation of vertical SOC variability index can be universally used for different post-mining soils under remediation, however, both proposed calculated indexes are unique for studied soils. The proposed model of an index may be helpful for remediation management, C sequestration prediction, and lowering the carbon footprint of mining activity.

Beneficial use of dredged sediments as a resource for mine reclamation: A case study of Lake Dianchi’s management in China

Dredging is one of the most effective methods for inhibiting the endogenous contamination of natural lakes. However, both the amount and the scope of dredging will be restricted if the disposal of the dredged sediment incurs considerable environmental and economic costs. The use of dredged sediments as a post-mining soil amendment for mine reclamation benefits both sustainable dredging and ecological restoration. This study incorporates a field planting experiment with a life cycle assessment to confirm the practical effectiveness of sediment disposal via mine reclamation, as well as its environmental and economic superiority over other alternative scenarios. The results show that the sediment offered plentiful organic matter and nitrogen for mine substrate, stimulating plant growth and increasing photosynthetic carbon fixation density, followed by enhanced plant root absorption and an improved soil immobilization effect on heavy metals. A 2:1 ratio of mine substrate to sediment is recommended to significantly promote the yield of ryegrass while reducing levels of groundwater pollution and soil contaminant accumulation. Due to the significant reduction in electricity and fuel, mine reclamation had minimal environmental impacts on global warming (2.63 × 10-2 kg CO2 eq./kg DS), fossil depletion (6.81 × 10-3 kg oil eq./DS), human toxicity (2.29 × 10-5 kg 1,4-DB eq/kg DS), photochemical oxidant formation (7.62 × 10-5 kg NOx eq./kg DS), and terrestrial acidification (6.69 × 10-5 kg SO2 eq./kg DS). Mine reclamation also had a lower cost (CNY 0.260/ kg DS) than cement production (CNY 0.965/kg DS) and unfired brick production (CNY 0.268/kg DS). The use of freshwater for irrigation and electricity for dehydration were the key factors in mine reclamation. Through this comprehensive evaluation, the disposal of dredged sediment for mine reclamation was verified to be both environmentally and economically feasible.

The Growth, Competition, and Facilitation of Grass and Legumes in Post-mining Soils

The Growth, Competition, and Facilitation of Grass and Legumes in Post-mining Soils

Post-Tin-Mining Agricultural Soil Regeneration Using Local Organic Amendments Improve Nitrogen Fixation and Uptake in a Legume–Cassava Intercropping System

The low nitrogen content of Bangka Island’s post-tin-mining soil may limit its suitability for agricultural production. In this study, we investigated the effect of locally available organic soil amendments on nitrogen fixation (N2–fixation) and crop nitrogen (N) uptake in a cassava–legume intercrop system. Cassava was intercropped with centrosema in post-tin-mining soils with six treatments, including a control and different soil amendments, such as dolomite, compost, charcoal, a combined treatment of charcoal and compost, and a combined treatment of compost and sawdust. The percentages of N derived from N2-fixation (%Ndfa) with the different seasons and treatments were comparable. Nonetheless, due to the higher shoot biomass accumulation, the mass of N2–fixation in soil amended with compost and when combined with charcoal was significantly higher than the control (50 to 73 kg ha−1). Treatments with compost and its combination with charcoal exhibited higher N uptake from the cassava–centrosema intercropped system (82 and 137 kg ha−1) and higher inorganic ammonium (NH4+) concentrations in the soil at harvest time (5.5 and 6.7 µg g−1). When combined with organic soil amendments, N2–fixation from centrosema produces not only higher biomass, but also higher N contribution to the system. Overall, locally available organic amendments, particularly the combined application of charcoal and compost, showed promise for improving N2–fixation of intercrop centrosema as well as for increasing N availability in the soil, which is of critical importance for crop growth in post-mining soils that have lost fertility.

Isolasi Cendawan Mikoriza Arbuskula dari Rizosfer Tanaman Berkayu Asal Pulau Bangka dan Karakteristik Struktur Kultur Mikorizanya

Arbuscular Mycorrhizal Fungi (AMF) associate with a variety of plants including forest trees. Research on AMF in forestry in Indonesia is limited, especially on woody plants grown in Bangka Island. Therefore, the aim of this research was to isolate and identify AMF associated with woody plants from Bangka Island grown in the post-mining soil in greenhouse for 7 years. The Soil samples derived from 8 pots of the 7 years old woody plants were used as spore sources. Spore extraction from the soil used the wet sieving and decanting method. Fungal identification was carried out based on morphological characteristics, and fungal isolation used pot culture with Pueraria javanica as a host plant. Fungal structures within the root were analyzed after root staining with trypan blue. There were 18 spore morphotypes observed which belong to 5 types of Acaulospora and 13 types of Glomus. Seven single spore pot cultures were successfully isolated from species of Acaulospora sp.1, Acaulospora sp.4, Acaulospora sp.5, Glomus sp.2, Glomus sp.3, Glomus sp.6, and Glomus sp.11. AM fungal colonization structures observed within the root were Paris type arbuscules, internal hyphae, and vesicles. The AMF cultures obtained could be used as biofertilizer for woody plant seedlings production for post mining reclamation activities.

The interactive effect of reclamation scenario and vegetation types on physical parameters of soils developed on carboniferous mine spoil heap

AbstractBulk density, porosity, and water retention capacity play a key role in limiting root growth and nutrient uptake in developed technogenic soils. Thus, the purpose of this study was to evaluate the impact of reclamation technologies and different vegetation types on vital physical soil properties formed from carboniferous materials. The case study was conducted on Sośnica hard coal post‐mine spoil heaps (Upper Silesian Coal Basin of Southern Poland). Nine experimental combinations of reclamation technologies and vegetation types were tested. For vegetation types and basic physical soil parameters, 120 plots (10 × 10 m) were selected. The texture analysis was done on composite soil samples collected at two uppermost soil depths (0–10 and 10–20 cm). Intact structure samples were collected into 100cm3 cylinders from the middle of each plot at both depths for bulk density (BD), porosity, capillary water capacity (CWC), and soil water storage (SWS). In the comparison of different reclamation scenarios, we noted that topsoiling increased BD, whereas CWC and porosity decreased. Spontaneous succession had the same effect on BD, porosity, and CWC as cultivation. Revegetation of the spoil heap with grasses and forbs resulted in lower BD and higher porosity and CWC in the top layer (0–10 cm), whereas woodland resulted in lower BD and higher porosity and CWC at lower depths (10–20 cm). The decreasing order of the SWS was grassland > forbland > woodland. Thus, forbs and grasses should be followed by tree planting, which is crucial to improving the hostile physical properties of the Carboniferous postmine soils.

Micromorphological soil assessment in abandoned quarry dumps of the Central Caucasus, Russia

This study compared the micromorphological and agrochemical metrics in soils from the quarry dumps and zonal soils, the Central Caucasus. Soil micromorphological investigations are important tool for evaluation of soil dynamics after anthropogenic impacts on terrestrial ecosystems. The results showed that the carbon content in the primary soil of the sand and gravel quarries was lower than that in the reference soil. The differences detected were statistically significant for both the Urvan plot soils (t = 11.95; p = 0.000) and the Progress plot soils (t = 18.73; p = 0.000). In contrast, in the quarry with clay bottom substrate (Gerpegezh), no significant difference was found between the reference and postmine soils. The reference soil around the sand and gravel quarries was slightly more acidic than the primary soil. In the clay quarry, the primary soil was more acidic with a strong acidic value, while the reference soil was neutral. The difference of nutrients (P, K, NH4+, NO3-) between the primary and reference soils were negligible. The only exception was the NO3- content in the reference soil of Progress settlement, where it was significantly higher (t = 4.19; p = 0.002) than in the original soil of the site. No difference was observed for the mineral component of the primary soil. Investigation of key zonal soils of the region. Zonal Caucasus soils: Phaeozem Gleiyc, Phaeozem and Umbric Retisol are different in terms of micro texture. Thus, Phaeozem Gleiyc characterizes by microstructure composed by primary angular mineral forms. Phaeozem and Retisol demonstrated formation of biogenic structure with alteration of mineral particles. Data obtained show that rapid self revegetation of the quarries results in initialization of primary soil formation and transformation of the soil microstructure and organization on the micro level.

Recovering of soil organic matter and associated C and N pools on regenerated forest ecosystems at different tree species influence on post-fire and reclaimed mine sites

Understanding the processes in soils after disturbances could be helpful in better designing restoration practices. The labile fraction of soil organic carbon (LSOC) plays a vital role in recovering soil properties and quality. This study compared labile C and N fractions, including microbial biomass C (MBC), cold-water-soluble C (WSC), water-soluble N (WSN), hot-water-extractable C (HWC), hot-water-extractable N (HWN), particulate organic carbon (POC), and particulate organic nitrogen (PON) in post-mining, post-fire, and undisturbed soils under different tree cover (Scots pine, common birch, and black alder). Nearly 30 years after the disturbance, post-mining soils were characterized by lower soil organic carbon (SOC) and total nitrogen (TN) as well as their labile fractions compared to those of post-fire and undisturbed soils. Disturbed soils had less stable SOC than undisturbed soils. Compared to the post-fire site, the higher proportion of WSC in SOC in post-mining soils suggests higher SOC turnover and leaching potential during primary succession compared to secondary succession. The post-fire soils had a similar SOC stock to undisturbed soils but differed in the C and N labile fractions stock. Post-fire soils were characterized by higher POC stocks due to charcoal admixture and lower WSN, HWC, and HWN fractions than undisturbed soils. The studied tree species differently affected the recovery of SOM properties after disturbances. Post-mining soils under alder had higher SOC, TN, and labile fractions (except MBC) than soils under birch and pine cover. However, this was not true for the post-fire site, where only WSN was higher under the alder. The soils under birch stands had the highest MBC stocks across all sites. The soils under pine had less stable SOM than those under birch and alder, which may accelerate podzolization processes. Results indicate a longer time for the recovery of C and N pools during primary succession than in secondary succession on sandy soils. Alder, as phytomelioration species that increases the C and N pools in post-fire sites, has limited usefulness.

ANALISIS EFEKTIVITAS ENDOMIKORIZA DAN AMELIORAN TERHADAP PEMBENTUKAN JARINGAN KAYU SEMAI JABON MERAH (Anthochepalus macropyllus)


 
 Arbuscular mycorrhizal fungi (FMA) is a potential microbial that can increase plant growth in various conditions of planting media, including in post-mining soil limestone. In the post-mining land, limestone has been found several types of FMA. This type of FMA has the potential as an inoculant source that is thought to increase the growth of the red jabon plant (anthocephalus macropyllus). Furthermore PT. Holcim Indonesia Tbk also produces the remaining cement laundering waste (LPS), which has the potential as ameliorating material that can increase the availability of nutrition, improve soil aggregate and increase the growth and formation of plant tissue (xylem). Considering that similar research is still minimal, it is necessary to research the effectiveness of using FMA inoculants and Amelioran material on the growth and formation of Red Jabon Semai Wood (A. Macropyllus) in the post-mining soil media. The varied analysis of variables using SPSS software version 10.01 was to determine the effect of treatment and the combination of treatment of the measured variables. At the same time, the analysis of wood anatomy uses the Sass method. The results showed that FMA and Amelioran soil effectively increased the growth and formation of wood networks in A. Macrophyllus. The interaction of FMA and LPS treatment can increase the proportion of phloem and xylem and is significant in increasing the proportion of empul. In contrast, the single FMA treatment effectively increases the growth of the proportion of phloem and xylem. Percentage of Increasing Floem Network Proposi M1 Treatment (64.91%), M2 (66.12%), and Increased Xylem Network Composition for M1 Treatment (16.59%) and M2 (16.38%).