Pore Size Of Matrix Research Articles

Effect of curing regimes on mechanical properties and microstructure of engineered cementitious composites with full desert sand

To establish a curing regime that facilitates the rapid enhancement and stable progression of early-age strength and tensile ductility in engineered cementitious composites with full desert sand (DSECC), aligning with production efficiency, this study examines the effects of different curing regimes (standard curing, natural curing, 20℃ water curing, and 90℃ water bath curing) on DSECC 's the mechanical properties and microstructure. The findings indicate that 90℃ water bath curing is beneficial to enhance the mechanical properties of DSECC, and its early 7 days strength and tensile deformation capacity (up to 7.05 %) can reach or exceed the standard curing 28 days level. In the uniaxial tensile test, samples cured in 20℃ water exhibit increased crack widths and reduced crack numbers, resulting in a sharp decline in the ultimate tensile strain from 4.96 % at 14 days to 1.57 % at 28 days. Natural curing leads to regression in strength at 28 days. The microstructure analysis revealed that 90℃ water bath curing fostered the development of high-density C-S-H gel and the dissolution of desert sand, while preserving the integrity of the fibers, enhancing the fiber-matrix interface transition zone (ITZ). The pore characteristic parameters under 90℃ water bath curing are superior, which has a notable refinement of the DSECC matrix's pore size. 20℃ Water curing can make up for the defects caused by desert sand and improve the compactness of matrix-aggregate ITZ. Natural curing yields the lowest degree of matrix hydration, with porosity reaching as high as 41.90 %, and pores smaller than 50 nm accounting for only 8.97 %.

Influence of Graphene Oxide Concentration and Ultrasonication Energy on Fracture Behavior of Nano-Reinforced Cement Pastes

The aim of this study is twofold. First, to assess the effect of the sonication process on the optimal dispersion of GO sheets for nanostructural reinforcement of cement pastes, as there is currently no clear criterion on this effect in the literature. For this purpose, in the first stage, the GO content in distilled water was fixed at 0.03% by weight, and the sheets were dispersed using different levels of ultrasonic energy, ranging from 0 J/mL to 2582 J/mL. In the second stage, to analyze the modification of pore structure due to the addition of GO sheets in different ratios (0–0.06% by weight) and its relationship with the mechanical and fracture properties of reinforced cement pastes. According to the results, it has been determined that the incorporation of GO sheets into the matrix alters the mechanical and fracture behavior, varying depending on matrix pore size and GO particle size. The addition of GO leads to a reduction in the average size of macropores (greater than 8 µm) of 13% for a dosage of 0.45% in weight and micropores (between 8 and 0.5 µm) in a 64% for the same composition with non-sonicated GO, although the total volume of pores in these ranges only decreased slightly. This reduction is more pronounced when the GO has not been sonicated and has larger particle size. Sonicated GO primarily modifies the range of capillary pores (<0.5 µm). The addition of GO with the highest degree of dispersion (465 nm) did not show significant improvements in compressive strength or Young’s modulus, as the cement used contains a significant volume of macropores that are not substantially reduced in any composition. Adding 0.030% ultrasonicated GO achieved a 7.8% increase in fracture energy, while an addition of 0.045% resulted in a 13.3% decrease in characteristic length, primarily due to the effect of capillary and micropores.

Study of the dual role mechanism triggered by in-situ cross-linking in hollow fiber membrane matrix

In this paper, a novel polyvinylidene fluoride (PVDF) hollow fiber membrane with uniform pore size and hydrophilic cross-linked network in membrane matrix was developed by non‐solvent induced phase separation method. The dual role mechanism of in-situ cross-linking in regulating molecular chain arrangement during phase separation and preventing deterioration of membrane mechanical properties were analyzed. The results demonstrated that the cross-linked network formed by in-situ crosslinking entangled with PVDF molecular chains which improved the regularity of PVDF molecular chains, increasing the uniformity of the pore size distribution, the percentage of the most probable pore size of the modified membrane increased to 93.25 %. The cross-linked network was immobilized in the membrane which formed semi-interpenetrating structure, maintaining the mechanical properties of the membrane. The permeation flux of the modified membrane increased by 24.1 times to 477.7 L m−2 h−1 compared to the original PVDF membrane. The rejection of BSA was maintained at 98.5 %, with the tensile strength increased to 2.52 MPa. The modified membrane exhibited persistent hydrophilicity in 21 days washing experiment with improved antifouling performance.

Fractal and Multifractal Analysis of Microscopic Pore Structure of UHPC Matrix Modified with Nano Silica

Nano silica (NS) has been found to have a positive impact on enhancing the microporous structure of Ultra-High-Performance Concrete (UHPC). However, there is a lack of effective methods to accurately characterize the regulatory improvement mechanism of NS on the pore structure of UHPC. In this study, our objective is to investigate the influence of NS on various characteristic parameters of the pore structure in UHPC, including porosity, average pore size, box fractal dimension, and multifractal spectral parameters. To analyze these effects, we employ a combination of X- CT image processing techniques and fractal theory. Furthermore, we conducted regression analysis using linear functions to explore the correlation between these parameters and the 28d compressive strength of UHPC. The experimental results demonstrate that NS promotes the refinement of matrix pore size, leading to a denser microstructure of the matrix. Fractal analysis revealed that the pore structure of NS-modified UHPC exhibited favorable fractal characteristics. The fractal dimension and multiple fractal parameters provided complementary insights into the pore structure of NS-modified UHPC from different perspectives. The fractal dimension described the global information, indicating that NS improved matrix defects and reduced the complexity of the pore structure. On the other hand, the multiple fractal parameters supplemented local information, highlighting how the increase in micropores contributed to the heterogeneity of the pore structure. The results of the correlation analysis indicate that the developed mathematical model has a good fit with the 28d compressive strength of UHPC.

Critical pressure (Pc) and critical temperature (Tc) of Midra shale

The critical pressure (Pc) and critical temperature (Tc) of shale gas depend on the characteristic pore size because of the importance of fluid–rock interactions in the matrix. This size dependency is neglected in highly permeable formations, where gas composition is only implemented because the fluid–fluid interactions are dominant. This study determines the critical properties by accounting for the characteristic pore size in the shale matrix and gas composition. The analyzed components are carbon dioxide, ethane, methane, n-butane, nitrogen, pentane, and propane. It shows that the bulk properties overestimate the actual critical properties. The overestimation varies between 15 and 26% in a uniform 5 nm conduit with a circular cross section, and it increases nonlinearly when decreasing the conduit size. Overestimation versus size is presented to provide a convenient tool for correcting the existing data. This study also determines the critical properties of Midra shale by accounting for the pore-throat size and pore-body size distributions. The former distribution is based on the mercury injection capillary pressure measurements of eight samples, whereas the latter is based on the nitrogen adsorption measurements of six samples. This study indicates that common bulk properties overestimate the critical properties of the studied shale between 5 and 22%. The results have applications in characterizing multiphase transport in shale gas reservoirs.

Shale barrier performance in petroleum systems: implications for CO 2 storage and nuclear waste disposal

Shale is often required to act as a natural barrier to fluid flow around nuclear waste repositories and above CO 2 storage sites. The small pore size of the shale matrix makes it an effective barrier to fluid flow. However, leakage could occur along faults or fractures. Experiments provide insight into fault/fracture-related leakage on short timescales (i.e. 1–10 years) compared to that needed for safe disposal (up to 1 Ma). Data collected by the petroleum industry provides strong evidence on how faults and fractures in shale impact fluid flow on such timescales. Faulted shales act as seals to petroleum reservoirs and abnormal pressures on geological timescales (>10 Ma). This observation suggests that faults in shale can either form without acting as flow conduits or act as temporary conduits but then reseal. Index properties such as clay content and elastic moduli are useful for identifying shales in which faults/fractures are likely to self-seal. However, fault and fracture-related fluid flow can occur through weak shales if high overpressures are maintained. Nuclear waste repositories can be sited away from where overpressures could develop. Leakage from CO 2 storage sites is more risky because the CO 2 provides drive to maintain high pressures, which could suppress self-sealing. Thematic collection: This article is part of the Fault and top seals 2022 collection available at: https://www.lyellcollection.org/topic/collections/fault-and-top-seals-2022

The Structure-Function Relationship of Branched Polyethylenimine Impregnated over Mesoporous Carbon Aerogels: An In-Depth Thermogravimetric Insight.

We present a comprehensive thermogravimetric analysis (TGA) of polyethylenimine (PEI)-impregnated resorcinol-formaldehyde (RF) aerogels. While numerous studies focus on PEI-impregnated SBA, RF materials have been less examined, despite their interest and specificities. As most articles on PEI-impregnated porous materials follow typical experimental methods defined for SBA, particularities of RF-PEI materials could remain unheeded. The design of nonisothermal TGA protocols, completed with nitrogen isotherms, based on the systematic filling of the matrix delivers a fundamental understanding of the relationship between the structure and function. This study demonstrates (i) the competition between the matrix and PEI for CO2-physisorption (φ) and CO2-chemisorption (χ), (ii) the hysteresis ([Formula: see text]) of CO2 capture at low temperature attributed to the kinetic (K) hindrance of CO2 diffusion (D) through PEI film/plugs limiting the chemisorption, and (iii) the thermodynamic (θ) equilibrium limiting the capture at high temperature. At variance with SBA-PEI materials, the first layers of PEI in RF are readily available for CO2 capture given that this matrix does not covalently bind PEI as SBA. A facile method allows the discrimination between physi- and chemisorption, exhibiting how the former decreases with PEI coverage. The CO2 capture hysteresis, while seldom introduced or discussed, underlines that the commonly accepted operating temperature of the "maximum capture" is based on an incomplete experiment. Through isotherm adsorption analysis, we correlate the evolution of this maximum to the morphological distribution of PEI. This contribution highlights the specificities of RF-PEI and the advantages of our TGA protocol in understanding the structure/function relationship of this kind of material by avoiding the typical direct applications of SBA-specific protocols. The method is straightforward, does not need large-scale facilities, and is applicable to other materials. Its easiness and rapidness are suited to high-volume studies, befitting for the comprehensive evaluation of interacting factors such as the matrix's nature, pore size, and PEI weight.

Drill-In Fluid Optimization for Formation Damage Control Considering Salt Dissolution in Saline-Lacustrine Reservoirs

Summary Salt dissolution induced by drill-in fluid loss is a frequent occurrence in saline-lacustrine reservoirs, which can potentially result in serious formation damage. In light of this, an experimental study was conducted to investigate the salt mineral dissolution and dynamic damage in the rock samples collected from a saline-lacustrine carbonate reservoir and the response of pore-fracture structures using the in-situ drill-in fluids. The study further involved analyzing the formation-damage-control (FDC) ability of the in-situ drill-in fluids. The results indicated that although salt dissolution significantly increased the pore size of the tight matrix and the width of natural fractures, improving the conductivity of seepage channels, the increase in pore-fracture size may have greatly aggravated the drill-in fluid loss during the process. The continuous serious filtrate loss, lower pressure-bearing capacity of the plugging zone, and lower permeability recovery rate (PRR) of rock indicated poor FDC performance of in-situ brine drilling fluids for the salt-dissolved core samples. The FDC performance of drill-in fluids for saline-lacustrine carbonate reservoirs was optimized based on the response of reservoir pore-fracture structure to salt dissolution and the theory of slightly underbalanced activity. The experimental results showed that the optimized drill-in fluids had better FDC ability, with an average PRR increase of 14.04%. Field application indicated that the optimized drill-in fluids reduced the drill-in fluid loss by 76.48%, shortened the drilling cycle by 45.20%, and increased the initial production capacity per well by 7.70%. This study can provide insightful guidance to optimize the FDC performance of drill-in fluids for saline-lacustrine hydrocarbon reservoirs during drilling.

Mechanical properties and shrinkage performance of one-part-geopolymer based on fly ash and micronized dredged sediments

This paper aims to study the drying and autogenous shrinkage of optimized One-Part-Geopolymer binders based on micronized dredged sediments and fly ash. Four One-Part-Geopolymer with 0, 15, 30 and 50% wt of sediments and fly ash were optimized by the variation of SiO2/Al2O3 molar ratio between 3; 3.5; 4; 4.5; 5. Findings shown that the sediments incorporation increases the mechanical strength and decreases the matrix pore size. This improvement is related to two phenomena: the fineness of the sediments which plays the role of nucleation zone in the matrix, and the coexistence of C-A-S-H and N-A-S-H gels as shown by the SEM-EDS. The reduction in the pore size of mortars with the addition of sediments leads to an increase in drying and autogenous shrinkage which have been linked to pore distribution rather than mass loss, unlike ordinary cementitious materials. However, the shrinkage values are still lower than the literature findings.

Modified experimental method to investigate micro transport in the shale matrix for shale gas production

Matrix permeability is the primary transport property for shale gas recovery, due to the special production process of gas from matrix to wellbore. With the micro pore size of shale matrix, it is necessary to consider micro transport mechanisms for the obtained permeability from macro experimental techniques. In this study, the macro experimental permeability was investigated in micro way, by the analyzation of pressure drop curves of shale matrix at high- and low-pressure experimental conditions, with modified numerical model containing micro transport mechanisms. As selected parameters, porosity, pore radius and tortuosity were regressed to obtain apparent permeabilities of three samples. The analysis shows that, tortuosity is much higher than macro one, and has been proved to be necessary and reasonable. The Knudsen diffusion and slippage effect control the micro transport of shale matrix at low and high pressure, separately, which is the main difference of macro and micro transports. Slippage flow weight factor helps to increase of slippage flow proportion with pressure. Higher pore radius and tortuosity both could weaken the effect of Knudsen diffusion, and slippage flow appears positive relationship with pore size at high pressure. This research provides an inspiration to shale transport study and a micro perspective theoretical guidance for the macro recovery of in-site shale gas reservoir.

Bifunctional polyhedral oligomeric silsesquioxane engineered polyamide membrane for efficient Li+/Mg2+ separation

Nanofiltration membrane technology holds great promise in extracting lithium (Li) from salt-lake brines. However, designing nanofiltration membranes with both high water permeance and ion selectivity remains a grand challenge. Here, amino-functionalized polyhedral oligomeric silsesquioxane (8NH2-POSS) nanocage is designed as a bifunctional molecular filler into polyamide (PA) layer during the interfacial polymerization of polyethyleneimine (PEI) and trimesoyl chloride (TMC). The 8NH2-POSS not only provides abundant amino groups to increase the membrane electropositivity from +4.5 mV to +33.2 mV (pH = 6.0), but also enlarges the pore size of PA matrix and constructs additional water channels to facilitate water transport. The optimal POSS-PA membrane has a rejection rate of 98.1 % to 1 g/L MgCl2, a pure water permeance of 12.0 L m-2h−1 bar−1 and an exceptional separation factor (SLi,Mg) of 13.9 at an ultralow Li+/Mg2+ mass ratio of 1:150, surpassing the benchmark PA membranes.

Comprehensive Characterization of Tissues Derived from Animals at Different Regenerative Stages: A Comparative Analysis between Fetal and Adult Mouse Skin.

Tissue regeneration capabilities vary significantly throughout an organism's lifespan. For example, mammals can fully regenerate until they reach specific developmental stages, after which they can only repair the tissue without restoring its original architecture and function. The high regenerative potential of fetal stages has been attributed to various factors, such as stem cells, the immune system, specific growth factors, and the presence of extracellular matrix molecules upon damage. To better understand the local differences between regenerative and reparative tissues, we conducted a comparative analysis of skin derived from mice at regenerative and reparative stages. Our findings show that both types of skin differ in their molecular composition, structure, and functionality. We observed a significant increase in cellular density, nucleic acid content, neutral lipid density, Collagen III, and glycosaminoglycans in regenerative skin compared with reparative skin. Additionally, regenerative skin had significantly higher porosity, metabolic activity, water absorption capacity, and elasticity than reparative skin. Finally, our results also revealed significant differences in lipid distribution, extracellular matrix pore size, and proteoglycans between the two groups. This study provides comprehensive data on the molecular and structural clues that enable full tissue regeneration in fetal stages, which could aid in developing new biomaterials and strategies for tissue engineering and regeneration.

Extracellular Matrix-Derived Biophysical Cues Mediate Interstitial Flow-Induced Sprouting Angiogenesis.

Sprouting angiogenesis is orchestrated by an intricate balance of biochemical and mechanical cues in the local tissue microenvironment. Interstitial flow has been established as a potent regulator of angiogenesis. Similarly, extracellular matrix (ECM) physical properties, such as stiffness and microarchitecture, have also emerged as important mediators of angiogenesis. However, the interplay between interstitial flow and ECM physical properties in the initiation and control of angiogenesis is poorly understood. Using a three-dimensional (3D) microfluidic tissue analogue of angiogenic sprouting with defined interstitial flow superimposed over ECM with well-characterized physical properties, we found that the addition of hyaluronan (HA) to collagen-based matrices significantly enhances sprouting induced by interstitial flow compared to responses in collagen-only hydrogels. We confirmed that both the stiffness and matrix pore size of collagen-only hydrogels were increased by the addition of HA. Interestingly, interstitial flow-potentiated sprouting responses in collagen/HA matrices were not affected when functionally blocking the HA receptor CD44. In contrast, enzymatic depletion of HA in collagen/HA matrices with hyaluronidase (HAdase) resulted in decreased stiffness, pore size, and interstitial flow-mediated sprouting to the levels observed in collagen-only matrices. Taken together, these results suggest that HA enhances interstitial flow-mediated angiogenic sprouting through its alterations to collagen ECM stiffness and pore size.

Evaluation of the Elastic Modulus and Plateau Stress of a 2D Porous Aluminum Alloy Based on a Convolutional Neural Network

Porous metals are a new ultra-light material with high specific stiffness, specific strength, and good energy absorption properties. The elastic modulus and plateau stress of porous metals are essential parameters. There have been many studies on the effects of the matrix material, porosity, and pore size on the elastic modulus and plateau stress of porous metals, but few studies can be found on the impact of pore arrangement. The pore arrangement of porous metals cannot be quantitatively described, and the design space of a porous metal structure under the same porosity is vast. With the powerful learning and prediction ability of neural networks, the influence of pore arrangement can be better understood. In this paper, a convolutional neural network was used to explore the impact of pore arrangement on both the elastic modulus and plateau stress of a porous aluminum alloy. Firstly, a finite element method was used to simulate the compression of a porous aluminum alloy to obtain a training sample library. Secondly, a convolutional neural network was built to positively predict the elastic modulus and plateau stress of the porous aluminum alloy. Partial samples were used to select the best training model from five convolutional neural network candidates. Dropout, Batch Normalization, and L2 regularization methods were used to alleviate the over-fitting phenomenon in training. All data in the database were then trained and predicted, and the predicted goodness of fit of the elastic modulus and plateau stress were 0.8785 and 0.5922, respectively. A search method based on the convolutional neural network was then used to iteratively search the database. Under the condition of using a small amount of data, the pore structure with the best elastic modulus and plateau stress in the database could be determined, and the inverse design of a structure with high elastic modulus and plateau stress could be realized.

The Impact of the Cellular Environment and Aging on Modeling Alzheimer's Disease in 3D Cell Culture Models.

Creating a cellular model of Alzheimer's disease (AD) that accurately recapitulates disease pathology has been a longstanding challenge. Recent studies showed that human AD neural cells, integrated into three-dimensional(3D) hydrogel matrix, display key features of AD neuropathology. Like in the human brain, the extracellular matrix (ECM) plays a critical role in determining the rate of neuropathogenesis in hydrogel-based 3D cellular models. Aging, the greatest risk factor for AD, significantly alters brain ECM properties. Therefore, it is important to understand how age-associated changes in ECM affect accumulation of pathogenic molecules, neuroinflammation, and neurodegeneration in AD patients and in vitro models.In this review, mechanistic hypotheses is presented to address the impact of the ECM properties and their changes with aging on AD and AD-related dementias. Altered ECM characteristics in aged brains, including matrix stiffness, pore size, and composition, will contribute to disease pathogenesis by modulating the accumulation, propagation, and spreading of pathogenic molecules of AD. Emerging hydrogel-based disease models with differing ECM properties provide an exciting opportunity to study the impact of brain ECM aging on AD pathogenesis, providing novel mechanistic insights. Understanding the role of ECM aging in AD pathogenesis should also improve modeling AD in 3D hydrogel systems.

Carbon quantum dots-Ag nanoparticle membrane for preventing emerging contaminants in oil produced water

A polycarbonate copolymer was used to create a novel low-cost microfiltration membrane. Due to their antibacterial properties, carbon quantum dots and silver nanoparticles (CD-Ag NPs) have been synthesized and incorporated into a poly(acrylonitrile-styrene) membrane by the Creighton method. This work examined the membranes using Infrared Vibrational Spectroscopy, Ultraviolet-Visible Spectroscopy, and a Scanning Electron Microscope (SEM). An investigation was conducted to determine the effects of the CDs-Ag NPs amount on the matrix's morphology, pore size, porosity, permeability, and mechanical strength. Also, photobleaching was used to reduce and stabilize CDs-Ag NPs, as reflected by a red shift in the spectra for CDs compared to CDs-Ag NPs from 290 nm to 570 nm in the UV/Vis spectrum, indicating the nanoparticle was generated. Pore size and mechanical strength are reduced when CDs-Ag NPs are added to neat membranes. However, it is followed by a decrease in porosity and mechanical strength. The optimized membrane exhibited a 0.66 pore size. In addition to removing oils and metals from wastewater, they also remove dyes, antibiotics, and other organic and inorganic compounds. The colony forming unit (CFU) test also showed that the percentage of CFU decreased as AgNO3 concentration increased so that at a concentration of 4, the percentage of CFU for E. coli and S. aureus was 5 % and 1.9 % for S. aureus. The Creighton methodology was therefore proven suitable for functionalizing the membrane, and discs-Ag NPs membranes have been demonstrated to be promising wastewater treatment membranes.

Synthesis, characterization and superoxide dismutase activity of a biomimetic Mn(III) complex covalently anchored to mesoporous silica

A mononuclear Mn(III) complex of a clickable ligand, [Mn(hbpapn)(H2O)2]ClO4·4.5H2O, where H2hbpapn = 1,3-bis[(2-hydroxybenzyl)(propargyl)amino]propane, has been prepared and fully characterized. The complex catalyzes the dismutation of superoxide employing a Mn(III)/Mn(IV) redox cycle, with catalytic rate constant of 3.9 × 106 M−1 s−1 determined through the nitro blue tetrazolium photoreduction inhibition assay, in aqueous medium of pH 7.8. The alkyne function of the ligand was used for the covalent attachment of the catalyst to azide modified mesoporous silicas with different texture and morphology, through click chemistry. In these materials the catalyst is essentially linked to the inner pore walls, isolated and protected from the external medium. The hybrid materials can be recycled, and retain or improve the superoxide dismutase activity of the free catalyst with the pore size of the solid matrix playing a role on the activity of the catalyst.

Synergistic effect of surfactants in porous geopolymer: Tailoring pore size and pore connectivity

With the growing interest in foamed geopolymer manufacture, there is an increasing demand for tailoring these composites’ pore structure. The binary system of two surfactants offers a possibility for enhancing foam formation efficiency and stability in geopolymer paste, however, their effects are not well understood. The influence of binary system blends on pore formation, size, and distribution in fly ash-based geopolymer matrix is investigated and their synergistic effects are evaluated. The results show that combining a nonionic surfactant with anionic Sodium Dodecyl Sulfate (SDS) increases open porosity, whereas cationic Cetyltrimethylammonium Bromide (CTAB) promotes the development of closed pores, improving thermal insulating, hygrothermal and mechanical performance. Moreover, this paper proposes a mechanism to describe the creation of pores in the presence of mixed micelles, as well as the benefit of employing mixed surfactant systems in customizing porous composites.

Electromigration Dispersion in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis of Proteins

The electromigration dispersion of the light- and heavy-chain subunit peaks of the therapeutic monoclonal antibody omalizumab was investigated in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using borate cross-linked dextran sieving matrices. Increasing boric acid content (340-640 mM) caused electromigration dispersion shifts for both low (2%)- and high (10%)-dextran-concentration gels in all gel-buffer compositions. In case of the heavy-chain fragment, elevated borate concentrations resulted in decreasing tailing and increasing fronting with the use of higher- and lower-dextran-concentration gels, respectively. The light-chain fragment, on the other hand, exhibited increased fronting with increasing borate concentration for both dextran concentrations examined in this study. Increase of the glycerol ingredient level in the gel-buffer system caused the same effect as the increasing borate concentration in both dextran concentrations. The detected electromigration dispersion was considered as the result of the formation of monomeric and dimeric glycerol-borate complexes as co-ionic constituents, migrating slower than that of the unconjugated tetrahydroxyborate. In addition, complexation of the tetrahydroxyborate anion with the glucose building blocks of the dextran polymer decreased its mobility to practically zero, contributing to further decrease in the resultant effective mobility of the co-ionic species. We suggest that the observed fronting and/or tailing peak shapes of the monoclonal antibody fragments in SDS-CGE at increasing boric acid concentrations can be considered as the result of multiple effects including changes in pH, sieving matrix pore size, viscosity, and the mobility variation of the co-ionic borate adducts with the gel-buffer ingredients. While electromigration dispersion-mediated band broadening, in general, can be minimized via matching the effective mobility of the co-ionic species to the analyte molecules of interest, in case of borate cross-linked dextran gels, optimization of the boric acid concentration required special consideration of its gel cross-linking function. For the light- and heavy-chain fragments of the IgG analyte, best peak shapes were attained with the use of 10% dextran/340 mM boric acid and 10% dextran/640 mM boric acid-containing gel-buffer systems, respectively. Based on this observation, here we introduce the concept of borate-gradient-mediated transient mobility matching in SDS-CGE of proteins. This novel approach resulted in close to optimal peak shapes for the distantly migrating IgG subunits within a single run, as well as unraveled the long-sought possible solution to perform capillary pore-size-gradient gel electrophoresis.

2.5D quartz fabric reinforced nanoporous phenolic composites with weakened heat transfer and optimized mechanical properties

Phenolic-based composites are the most promising ablative thermal protection materials for space applications, but optimizing their thermal insulation performance while maintaining high strength remains extremely challenging. Herein, phenolic-based composites with co-optimized thermal insulation and mechanical properties are prepared via 2.5D quartz fabric reinforcing nanoporous phenolic. The pore size of phenolic matrix is efficiently refined to ∼35 nm by adjusting resin concentration, enabling composites to exhibit high density but low thermal conductivity by enhancing Knudsen diffusion. Finite element analysis shows that low braiding angle of 2.5D fabric can further weakening heat transfer of composites due to anisotropic thermal conductivity of fiber yarns. In-situ X-ray micro-CT results indicate that 2.5D fabrics can reinforce composites through interwoven yarns, and straighter weft yarns result in higher strength along weft direction than that along warp direction. Compared with dense quartz/phenolic composite, the resulting composites exhibit 16.5% lower density (1.32 g/cm3), 58.8% lower thermal conductivity (0.21 W/(m·K), ≥372.7% higher tensile strength (≥182.0 ± 9.6 MPa) and comparable ablation resistance. The present results will further advance the application of phenolic-based composites in more extreme re-entry environments.