Porcine Muscle Tissue Research Articles

Characterizing Temperature-Dependent Acoustic and Thermal Tissue Properties for High-Intensity Focused Ultrasound Computational Modeling

High-intensity focused ultrasound (HIFU) thermal therapies utilize concentrated sound waves to ablate diseased tissue at precise locations within the body. Computational simulations of HIFU can assist clinicians by predicting the death of target tissues, identifying sensitive healthy tissues that risk thermal damage, and optimizing acoustic power delivery to minimize treatment times and maximize treatment efficacy. Accurate simulations require accurate inputs, and many computational solvers neglect property changes induced by tissue heating during treatment. Additionally, temperature-dependent tissue property data in the literature are relatively scarce. This study presents methodology for characterizing temperature-dependent acoustic and thermal properties in ex vivo porcine muscle tissue. From 20 – 50 °C, speed of sound is found to increase from approximately 1580 – 1620 m/s. The acoustic attenuation coefficient increases for 20 – 50 °C from 0.09 – 0.24 Np/cm at 0.5 MHz and 0.16 – 0.37 Np/cm at 1.6 MHz. Thermal conductivity and thermal diffusivity increase from 0.52 – 0.55 W/m °C and 0.147 – 0.158 mm2/s, respectively, over 20 – 60 °C. Specific heat capacity increases from approximately 3500 – 3800 J/kg °C, over 20 – 80 °C. Each property is consistent with data found in the literature, extends the literature to a larger temperature range, and, for acoustic properties, extends to unique frequencies. Temperature-dependent predictive models are also developed for each of the five properties. This study’s property measurement methodologies can be used to characterize other biological tissues, and the predictive models developed herein will facilitate future efforts in temperature-dependent modeling and uncertainty quantification of HIFU thermal therapies.

Wireless Magnetic Robot for Precise Hierarchical Control of Tissue Deformation.

Mechanotherapy has emerged as a promising treatment for tissue injury. However, existing robots for mechanotherapy are often designed on intuition, lack remote and wireless control, and have limited motion modes. Herein, through topology optimization and hybrid fabrication, wireless magneto-active soft robots are created that can achieve various modes of programmatic deformations under remote magnetic actuation and apply mechanical forces to tissues in a precise and predictable manner. These soft robots can quickly and wirelessly deform under magnetic actuation and are able to deliver compressing, stretching, shearing, and multimodal forces to the surrounding tissues. The design framework considers the hierarchical tissue-robot interaction and, therefore, can design customized soft robots for different types of tissues with varied mechanical properties. It is shown that these customized robots with different programmable motionscan induce precise deformations of porcine muscle, liver, and heart tissues with excellent durability. The soft robots, the underlying design principles, and the fabrication approach provide a new avenue for developing next-generationmechanotherapy.

Low-velocity nail penetration response of muscle tissue and gelatin

Quantitative estimation of soft tissue injuries due to penetration of sharp objects is a challenging task for forensic pathologists. The severity of injury depends on the force required to penetrate the tissue. This study focuses on investigating the amount of force required to penetrate porcine muscle tissue and gelatin simulants (10 % wt) to mimic human muscle tissue when subjected to sharp objects like nail at velocities below 5 m/s. A custom-made experimental setup was used to examine the influence of penetration velocity and nail diameter on penetration forces. Images captured by a high-speed camera were used to track the position and velocity of the nail. A finite element (FE) model was established to simulate the penetration behavior of the tissue and gelatin. The FE simulations of the nail penetration were validated through direct comparison with the experimental results. In tissues as well as in the simulant, penetration forces were seen to increase with increasing nail diameter and velocity. Porcine muscle tissue showed 23.9–46.5 % higher penetration forces than gelatin simulants (10 % wt) depending on nail diameter and velocity; the difference being higher for higher nail diameter and velocity. The ranges of maximum penetration forces measured were 8.6–59.1 N for porcine muscle tissue and 6.8–34.9 N for gelatin simulant. This study helps to quantify injuries caused by sharp nails at low velocities and offers insights with potential applications in injury management strategies and forensic studies.

Development of 3D printed tissue-mimicking materials: Combining fiber reinforcement and fluid content for improved surgical rehearsal

The prevalence of medical errors during surgical procedures has led to a higher emphasis on improving surgical outcomes, by improving surgical planning and training. Anatomical models have become valuable tools for pre-operative planning and current 3D printed models strive to better match real soft biological tissues. This study aimed to develop novel 3D printed material composites with controllable mechanical properties that mimic soft tissue. Concepts of microstructuring, fiber reinforcement and fluid infill in extrusion-based 3D printing are combined to design tunable materials towards target tissues of porcine muscle and liver. Material characterization was performed in triangular wave cyclic experiments under uniaxial tension with increasing displacements. Hereby, initial EI and final EII elastic moduli were evaluated. Further, the viscous response was characterized by the dissipated energy ratio UD and suture retention strength (SRS) was determined by single tensile pull-out tests Elastic moduli of printed materials were successfully tuned to 510 ± 10 kPa, closely resembling porcine muscle with 580 ± 150 kPa. The dissipated energy ratio UD of the silicone was increased from 0.09 ± 0.01 to 0.46 ± 0.17 by addition of gyroid infill and viscous fluid. Suture retention strength (SRS) for porcine liver tissue was 1.64 ± 0.42 N, while that of 3D printed silicone showed a mean SRS of 5.1 ± 0.6 N. Although the exact properties of porcine muscle and liver tissue require finer tuning, this study established techniques for refinement of 3D printed tissue-mimicking materials, ultimately enabling more accurate models for surgical rehearsal.

Heating of metallic orthodontic devices during anti-aging treatment with vacuum and electromagnetic fields: In vitro study.

The physical appearance of an individual plays a primary role as it influences the opinion of the viewer. For this reason, orthodontic therapy to improve perceived aesthetics is in high demand among patients. This factor, combined with the increase in the number of non-invasive facial aesthetic treatments, has led to the need to understand potential risk factors in the application of medical devices to the perioral skin in patients with fixed orthodontic appliances. The aim of this study was to evaluate in vitro heating of the orthodontic bracket following electromagnetic fields and negative pressure (V-EMF) used as an anti-aging treatment. Two different types of titanium alloy wires, one made of "beta-Titanium" alloy and the other "Ni-Ti" (DW Lingual Systems GmbH-Bad Essen-Germany) were used. The orthodontic wires and brackets mounted on a resin mouth were covered with porcine muscle tissue, then subjected to anti-aging therapy with a Bi-one LifeTouchTherapy medical device (Expo Italia Srl-Florence-Italy) which generates a combination of vacuum and electromagnetic fields (V-EMF) already adopted for antiaging therapy. During administration of the therapy, the orthodontic brackets and porcine tissue were thermally monitored using a Wavetek Materman TMD90 thermal probe (Willtek Communications GmbH-Germany). In total 20 orthodontic mouths were used, 10 with Beta Titanium wires and 10 with Nickel Titanium wires. A temperature increase of about 1°C was recorded in each group. The outcome of the present research shows that the absolute temperatures measured on orthodontic appliances, which, despite having a slightly different curve, both show an increase in temperature of 1.1°C at the end of the session, thus falling well within the safety range of 2°C as specified by the standard CENELEC EN 45502-1. Therefore, V-EMF therapy can be considered safe for the entire dental system and for metal prostheses, which tend to heat up at most as much as biological tissue (+0.9°C/1.1°C vs. 1.1°C/1.1°C). In conclusion, anti-aging therapy with V-EMF causes a thermal increase on orthodontic brackets that is not harmful to pulp health.

Super-resolution stimulated Raman scattering microscopy enhanced by quantum light and deconvolution

Stimulated Raman scattering (SRS) microscopy is a powerful tool for label-free chemical contrast bio-imaging. However, its spatial resolution is limited by diffraction; its noise level is also fundamentally limited by the shot noise due to the quantum nature of light. In this work, we apply the squeezed light technique associated with the deconvolution method to achieve quantum-enhanced super-resolution SRS microscopy. To generate squeezed pump light, we design a unique cascaded scheme by using two nonlinear crystals, in which the second-harmonic generation (SHG) from the first crystal is used to boost the SHG of the second crystal sequentially. Such a cascaded light squeezed scheme suppresses the shot noise down to 89.7% (1 dB), which can be readily applied to the existing conventional SRS microscopy. We combine the squeezed light-controlled SRS with the Richardson–Lucy deconvolution method to break the diffraction limit by improving the spatial resolution of ∼2.2-fold compared to conventional SRS imaging. We realize the quantum-enhanced super-resolution SRS imaging in a variety of samples (e.g., oleic acid, porcine muscle tissue), suggesting the potential of squeezed light SRS with deconvolution for label-free super-resolution chemical imaging in biological and biomedical systems.

Photothermal Effects of High-Energy Photobiomodulation Therapies: An In Vitro Investigation.

The purpose of this study was to investigate photothermal aspects of photobiomodulation therapies (PBMT) in vitro to assist in the development of safe clinical parameters with respect to higher-power devices with large surface applicators. Laser wavelengths in the range of 650 nm-1064 nm were investigated using a thermal camera. Thermographic measures of surface and sub-surface temperature variations of similar lean porcine muscle tissue samples were recorded for a series of calibrated experiments. A thermal comparison was then made between Flat-top and Gaussian beam spatial distribution devices. Outcome data were subjected to statistical analysis using an ANOVA model. Results acquired at similar parameters of irradiance indicated that the application of the 980 nm wavelength was associated with the highest rise in temperature, which decreased with other wavelengths in the order 980 > 1064 ≈ 650 >>> 810 nm (p < 5 × 10-20). All wavelengths assessed were associated with a significant temperature increase, and with the exception of 810 nm, all exceeded the threshold of a 6 °C rise within the prescribed parameter limits. Optical scanning by movement of the applied source over a relevant area was found to offer effective mitigation of these temperature increases. An extended discussion is presented, analysing the clinical significance of the study outcomes. Recommendations are made within the limits of this in vitro study in order to assist future clinical investigations.

Minimally Invasive Live Tissue High-Fidelity Thermophysical Modeling Using Real-Time Thermography.

We present a novel thermodynamic parameter estimation framework for energy-based surgery on live tissue, with direct applications to tissue characterization during electrosurgery. This framework addresses the problem of estimating tissue-specific thermodynamics in real-time, which would enable accurate prediction of thermal damage impact to the tissue and damage-conscious planning of electrosurgical procedures. Our approach provides basic thermodynamic information such as thermal diffusivity, and also allows for obtaining the thermal relaxation time and a model of the heat source, yielding in real-time a controlled hyperbolic thermodynamics model. The latter accounts for the finite thermal propagation time necessary for modeling of the electrosurgical action, in which the probe motion speed often surpasses the speed of thermal propagation in the tissue operated on. Our approach relies solely on thermographer feedback and a knowledge of the power level and position of the electrosurgical pencil, imposing only very minor adjustments to normal electrosurgery to obtain a high-fidelity model of the tissue-probe interaction. Our method is minimally invasive and can be performed in situ. We apply our method first to simulated data based on porcine muscle tissue to verify its accuracy and then to in vivo liver tissue, and compare the results with those from the literature. This comparison shows that parameterizing the Maxwell-Cattaneo model through the framework proposed yields a noticeably higher fidelity real-time adaptable representation of the thermodynamic tissue response to the electrosurgical impact than currently available. A discussion on the differences between the live and the dead tissue thermodynamics is also provided.

Magnetic Resonance Acoustic Radiation Force Imaging (MR-ARFI) for the monitoring of High Intensity Focused Ultrasound (HIFU) ablation in anisotropic tissue.

We introduce a non-invasive MR-Acoustic Radiation Force Imaging (ARFI)-based elastography method that provides both the local shear modulus and temperature maps for the monitoring of High Intensity Focused Ultrasound (HIFU) therapy. To take tissue anisotropy into account, the local shear modulus μ is determined in selected radial directions around the focal spot by fitting the phase profiles to a linear viscoelastic model, including tissue-specific mechanical relaxation time τ. MR-ARFI was evaluated on a calibrated phantom, then applied to the monitoring of HIFU in a gel phantom, ex vivo and in vivo porcine muscle tissue, in parallel with MR-thermometry. As expected, the shear modulus polar maps reflected the isotropy of phantoms and the anisotropy of muscle. In the HIFU monitoring experiments, both the shear modulus polar map and the thermometry map were updated with every pair of MR-ARFI phase images acquired with opposite MR-ARFI-encoding. The shear modulus was found to decrease (phantom and ex vivo) or increase (in vivo) during heating, before remaining steady during the cooling phase. The mechanical relaxation time, estimated pre- and post-HIFU, was found to vary in muscle tissue. MR-ARFI allowed for monitoring of viscoelasticity changes around the HIFU focal spot even in anisotropic muscle tissue.

An efficient and economical way to obtain porcine muscle stem cells for cultured meat production

Cultured meat technology is an emerging and promising strategy for animal protein production. Muscle stem cells are regarded as important seed cells for generating muscle fiber in vitro because of their proliferative and myogenic differentiation potential. However, current approaches for the isolation and purification of muscle stem cells are low-yield and high-cost, limiting the industrial production of cultured meat. Here, we reported an efficient and economical protease combination consisting of pronase and dispase II for the isolation of primary muscle stem cells, achieving 5.06 ± 0.12 × 106 nucleated cells and 3.19 ± 0.19 × 106 Pax7+ cells from 1 g of porcine muscle tissue. Furthermore, by investigating the effect of initial purity on the proliferation and differentiation potential of muscle stem cells, we found that higher purity of initial muscle stem cells promoted the maintenance of myogenic properties of cells after expansion but reduced the total number of obtained cells. Based on nucleated cells isolated from 1 g of porcine muscle, muscle stem cells purified by 0.5 h of pre-plating yielded 2.19 ± 0.16 × 108 cells with myogenic differentiation capacity after 20 days of expansion, which was 5-fold higher than those purified by fluorescence-activated cell sorting (FACS). Therefore, a modified approach was developed to obtain porcine muscle stem cells for cultured meat production, involving tissue digestion with the pronase and dispase II combination and purification through pre-plating for 0.5 h. This approach was simple, efficient, and economic, which would facilitate the industrial production of cultured meat.

Fracture toughness determination of porcine muscle tissue based on AQLV model derived viscous dissipated energy

The ability of soft collagenous tissue (SCT) to withstand propagation of a defect in the presence of a macroscopic crack is termed the ’fracture toughness parameter’. In soft tissues not undergoing significant plastic deformation, it is purported that a considerable amount of additional energy is dissipated during failure processes, due to viscoelasticity. Hence the total work, measured experimentally during failure, is the sum of fracture and viscoelastic energies. Previous authors have aimed to apply constitutive modeling to describe viscoelastic hysteresis for fracture toughness determination with a tendency of models to either over or underestimate the viscous energy. In this study, the fracture toughness of porcine muscle tissue is determined using two strategies. Firstly, it was determined experimentally by calculation of the difference in dissipated energy of notched and unnotched tissue specimens undergoing cyclic ’triangular wave’ excitation with increasing strain levels in uniaxial tension. The second strategy involved the extension and use of the adaptive quasi-linear viscoelastic model (AQLV) to model cyclic loading (model parameters were obtained from a previous study) and sequentially the dissipated energy was calculated. The mean value of the dissipated energy based on the AQLV approach was then subtracted from the total dissipated energy of notched porcine muscle tissue samples to determine the fracture toughness. The mean experimental viscous dissipated energy ratio was 0.24 ± 0.04 in the experimental approach, compared to 0.28 ± 0.03 for the AQLV model. Fracture toughness determined experimentally yielded 0.84 ± 0.80 kJ/m2, and 0.71 ± 0.76 kJ/m2 for the AQLV model, without a significant difference (p = 0.87). Hence, the AQLV model enables a reasonable estimation of viscous dissipated energy in porcine muscle tissue with the advantage to perform tests only on notched specimens, instead of testing additional unnotched samples. Moreover, the AQLV model will help to better understand the constitutive viscoelastic behaviour of SCTs and might also serve as a basis for future fracture toughness determination with constitutive model simulations.

Optical Property Measurement and Temperature Monitoring in High-Intensity Focused Ultrasound Therapy by Diffuse Optical Tomography: A Correlation Study

In this article, we propose a new approach utilizing diffuse optical tomography (DOT) to monitoring the changes in tissues’ optical properties and temperature in high-intensity focused ultrasound (HIFU) therapy. By correlating the tissue reduced scattering coefficient (μs’) reconstructed by DOT and the temperature measured by a thermocouple, the quantitative relationship between μs’ and temperature in HIFU treatment was explored. The experiments were conducted using porcine and chicken breast muscle tissues during HIFU; the temperature of each tissue sample was recorded using a thermocouple. To incorporate the temperature dependency of tissue optical properties, both polynomial and exponential models were utilized to fit the experimental data. The results show that the change of μs’ during HIFU treatment could be detected in real-time using DOT and that this change of μs’ is quantitatively correlated with tissue temperature. Furthermore, while the tissue-type-dependent relationship between μs’ and temperature is non-linear in nature, it is stable and repeatable. Therefore, our approach has the potential to be used to predict temperature of tissue during HIFU treatment.

Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3.

Chimeric RNA is a crucial target for tumor diagnosis and drug therapy, also having its unique biological role in normal tissues. TNNI2-ACTA1-V1 (TA-V1), a chimeric RNA discovered by our laboratory in porcine muscle tissue, can inhibit the proliferation of Porcine Skeletal Muscle Satellite Cells (PSCs). The regulatory mechanism of TA-V1 in PSCs remains unclear, but we speculate that NCOA3, DDR2 and RDX may be the target genes of TA-V1. In this study, we explored the effects of NCOA3, DDR2 and RDX on cell viability and cell proliferation by CCK-8 assay, EdU staining and flow cytometry. Furthermore, the regulatory pathway of proliferation in PSCs mediated by TA-V1 through NCOA3 or CyclinD1 was elucidated by co-transfection and co-immunoprecipitation (Co-IP). The results revealed that overexpression of NCOA3 significantly increased cell viability and the expression level of CyclinD1, and also promotes cell proliferation by changing cells from the G1 phase to the S phase. In addition, inhibiting the expression of NCOA3 substantially reduced cell viability and inhibited cell proliferation. Overexpression of DDR2 and RDX had no significant effect on cell viability and proliferation. Co-transfection experiments showed that NCOA3 could rescue the proliferation inhibition of PSCs caused by TA-V1. Co-IP assay indicated that TA-V1 directly interacts with NCOA3. Our study explores the hypothesis that TA-V1 directly regulates NCOA3, indirectly regulating CyclinD1, thereby regulating PSCs proliferation. We provide new putative mechanisms of porcine skeletal muscle growth and lay the foundation for the study of chimeric RNA in normal tissues.

Production of cultured meat by culturing porcine smooth muscle cells in vitro with food grade peanut wire-drawing protein scaffold

Cultivating meat is a promising solution to the negative problems brought by traditional animal husbandry. To make cultured meat have the sensory and nutritional characteristics of conventional meat as much as possible, many studies have been conducted on various cell types and scaffold characteristics. Therefore, this study aims to produce a low-cost cultured meat with a quality closer to that of conventional meat. Tissue generation requires three-dimensional (3D) scaffolds to support cells and simulate extracellular matrix (ECM). Here, we used peanut wire-drawing protein (a biomaterial based on edible porous protein) as a new culture meat scaffold to culture cells. The scaffold can support cell attachment and proliferation to create 3D engineered porcine muscle tissue. The differentiation of smooth muscle cells (SMCs) was induced by a low serum medium to produce more extracellular matrix proteins. After differentiation, it was found that peanut wire-drawing protein scaffolds could be used for porcine smooth muscle cell adhesion and growth. The ECM protein and muscle protein produced by SMCs can endow cultured meat with better quality. This technology provides an innovative pathway for the industrialized production of cultured meat.

Next Generation Sequencing-based DNA metabarcoding for animal species profiling in fish feed

The expansion of worldwide aquaculture has been accompanied by extensive growth in the fish feed industry. However, improper labelling of many commercially available fish feeds has raised security and safety concerns over the species’ origin of the ingredients. The inclusion of ruminants-derived ingredients in fish feed is prohibited according to EU legislation while porcine inclusion in fish feed has been a great concern among Muslim farmers. In contrast to the limited species that could be simultaneously determined using multiplex PCR, this study utilised Next Generation Sequencing-based DNA metabarcoding assay to determine the compositional profiles of animal species in fish feed samples in a more holistic manner. In relation to the religious issue associated with porcine-derived ingredients in fish feed, this study firstly aimed to determine the sensitivity of the methods in profiling fish feed adulterated with porcine blood and muscle tissues. Next, 10 commercially available fish feed samples were analysed. As a result, a detection limit of as low as 3% (w/w) porcine muscle and blood in the laboratory-prepared fish feed was obtained. The analysis of 10 commercial fish feeds shows surprising findings: 50% of the feeds contain Sus scrofa and 80% contain Bos taurus, a ruminant. Only one commercial fish feed was found to be solely composed of marine species. This study shows that commercial fish feeds sold in Malaysia contain undesirable animal species, and emphasises the need for accurate and legally enforced labelling of mammalian species in fish feed products.

Tissue-mimicking phantom materials with tunable optical properties suitable for assessment of diffuse reflectance spectroscopy during electrosurgery.

Emerging intraoperative tumor margin assessment techniques require the development of more complex and reliable organ phantoms to assess the performance of the technique before its translation into the clinic. In this work, electrically conductive tissue-mimicking materials (TMMs) based on fat, water and agar/gelatin were produced with tunable optical properties. The composition of the phantoms allowed for the assessment of tumor margins using diffuse reflectance spectroscopy, as the fat/water ratio served as a discriminating factor between the healthy and malignant tissue. Moreover, the possibility of using polyvinyl alcohol (PVA) or transglutaminase in combination with fat, water and gelatin for developing TMMs was studied. The diffuse spectral response of the developed phantom materials had a good match with the spectral response of porcine muscle and adipose tissue, as well as in vitro human breast tissue. Using the developed recipe, anatomically relevant heterogeneous breast phantoms representing the optical properties of different layers of the human breast were fabricated using 3D-printed molds. These TMMs can be used for further development of phantoms applicable for simulating the realistic breast conserving surgery workflow in order to evaluate the intraoperative optical-based tumor margin assessment techniques during electrosurgery.

A Novel miRNA Y-56 Targeting IGF-1R Mediates the Proliferation of Porcine Skeletal Muscle Satellite Cells Through AKT and ERK Pathways.

As a key regulator of gene transcription and post-transcriptional modification, miRNAs play a wide range of roles in skeletal muscle development. Skeletal muscle satellite cells contribute to postnatal growing muscle fibers. Thus, the goal of this study was to explore the effects of novel miRNA Y-56 on porcine skeletal muscle satellite cells (PSCs). We found that Y-56 was highly expressed in porcine muscle tissues, and its expression was higher in Bama Xiang pigs than in Landrace pigs. The EdU assay, cell counting kit-8, and flow cytometry results showed that Y-56 overexpression suppressed cell proliferation and cell cycle, whereas Y-56 inhibition resulted in the opposite consequences. The results of qRT-PCR and Western blot showed that Y-56 remarkably inhibited the expression levels of cyclin-dependent kinase 4 (CDK4), proliferating cell nuclear antigen (PCNA), and cyclin D1. We identified that IGF-1R was a direct target of Y-56 by dual-luciferase reporter assay. Moreover, IGF-1R overexpression promoted the proliferation and cell cycle process of PSCs and upregulated the expression of CDK4, PCNA, and cyclin D1. Conversely, IGF-1R knockdown had the opposite effect. Furthermore, IGF-1R overexpression partially reversed the inhibition of the cell proliferation and cell cycle process of PSCs and the downregulation of the expression of CDK4, PCNA, and Cyclin D1 caused by Y-56 overexpression. Finally, Y-56 inhibited the protein expression levels of p-AKT and p-ERK. Collectively, our findings suggested that Y-56 represses the proliferation and cell cycle process of PSCs by targeting IGF-1R-mediated AKT and ERK pathways.

Angiogenic Hyaluronic Acid Hydrogels with Curcumin-Coated Magnetic Nanoparticles for Tissue Repair.

Angiogenic magnetic hydrogels are attractive for tissue engineering applications because their integrated properties can improve angiogenesis while providing magnetic guidance and stimulation for tissue healing. In this study, we synthesized magnetic nanoparticles (MNPs) with curcumin as an angiogenic agent, referred to as CMNPs, via a one-pot coprecipitation method. We dispersed CMNPs in hyaluronic acid (HyA) to create angiogenic magnetic hydrogels. CMNPs showed a slightly reduced average diameter compared to that of MNPs and a curcumin content of 11.91%. CMNPs exhibited a sustained slow release of curcumin when immersed in a revised simulated body fluid (rSBF). Both CMNPs and MNPs showed a dose-dependent cytocompatibility when cultured with bone marrow-derived mesenchymal stem cells (BMSCs) using the direct exposure culture method in vitro. The average BMSC density increased when the concentrations of CMNPs or MNPs increased from 100 to 500 μg/mL, but the cell density decreased when the nanoparticle concentration reached 1000 μg/mL. CMNPs showed a weaker magnetic response than MNPs both in air and in water immediately after synthesis but retained the magnetism better than MNPs when embedded in the HyA hydrogel because of less oxidation. CMNPs were able to respond to magnetic guidance even when the porcine skin or muscle tissues were placed in between the nanoparticles and external magnet. The magnetic hydrogels of HyA_CMNP and HyA_MNP promoted the adhesion of BMSCs in a direct exposure culture. The HyA_CMNP group also showed the highest secretion of the vascular endothelial growth factor with the release of curcumin in vitro. Overall, our magnetic hydrogels integrated the desirable properties of cytocompatibility and angiogenesis with magnetic guidance, thus proving to be promising for improving tissue regeneration.

Fracture Toughness Determination of Porcine Muscle Tissue Based on Aqlv Model Derived Viscous Dissipated Energy

Fracture Toughness Determination of Porcine Muscle Tissue Based on Aqlv Model Derived Viscous Dissipated Energy

A parameter reduced adaptive quasi-linear viscoelastic model for soft biological tissue in uniaxial tension

Mechanical characterisation of soft viscous materials is essential for many applications including aerospace industries, material models for surgical simulation, and tissue mimicking materials for anatomical models. Constitutive material models are, therefore, necessary to describe soft biological tissues in physiologically relevant strain ranges. Hereby, the adaptive quasi-linear viscoelastic (AQLV) model enables accurate modelling of the strain-dependent non-linear viscoelastic behaviour of soft tissues with a high flexibility. However, the higher flexibility produces a large number of model parameters.In this study, porcine muscle and liver tissue samples were modelled in the framework of the originally published AQLV (3-layers of Maxwell elements) model using four incremental ramp-hold experiments in uniaxial tension. AQLV model parameters were reduced by decreasing model layers (M) as well as the number of experimental ramp-hold steps (N).Leave One out cross validation tests show that the original AQLV model (3M4N) with 19 parameters, accurately describes porcine muscle tissue with an average R2 of 0.90 and porcine liver tissue, R2 of 0.86. Reducing the number of layers (N) in the model produced acceptable model fits for 1-layer (R2 of 0.83) and 2-layer models (R2 of 0.89) for porcine muscle tissue and 1-layer (R2 of 0.84) and 2-layer model (R2 of 0.85) for porcine liver tissue. Additionally, a 2 step (2N) ramp-hold experiment was performed on additional samples of porcine muscle tissue only to further reduce model parameters. Calibrated spring constant values for 2N ramp-hold tests parameters k1 and k2 had a 16.8% and 38.0% deviation from those calibrated for a 4 step (4N) ramp hold experiment. This enables further reduction of material parameters by means of step reduction, effectively reducing the number of parameters required to calibrate the AQLV model from 19 for a 3M4N model to 8 for a 2M2N model, with the added advantage of reducing the time per experiment by 50%.This study proposes a ’reduced-parameter’ AQLV model (2M2N) for the modelling of soft biological tissues at finite strain ranges. Sequentially, the comparison of model parameters of soft tissues is easier and the experimental burden is reduced.