Populus Purdomii Research Articles

Multinuclei Occurred Under Cryopreservation and Enhanced the Pathogenicity of Melampsora larici-populina.

Melampsora larici-populina is a macrocyclic rust, and the haploid stage with two nuclei and the diploid of mononuclear sequentially occur annually. During the preservation of dry urediniospores at −80°C, we found that one isolate, ΔTs06, was different from the usual wild-type isolate Ts06 at −20°C because it has mixed polykaryotic urediniospores. However, the other spores, including the 0, I, III, and IV stages of a life cycle, were the same as Ts06. After five generations of successive inoculation and harvest of urediniospores from the compatible host Populus purdomii, the isolate ΔTs06 steadily maintained more than 20% multiple nucleus spores. To test the pathogenesis variation of ΔTs06, an assay of host poplars was applied to evaluate the differences between ΔTs06 and Ts06. After ΔTs06 and Ts06 inoculation, leaves of P. purdomii were used to detect the expression of small secreted proteins (SSPs) and fungal biomasses using quantitative real-time PCR (qRT-PCR) and trypan blue staining. ΔTs06 displayed stronger expression of five SSPs and had a shorter latent period, a higher density of uredinia, and higher DNA mass. A transcriptomic comparison between ΔTs06 and Ts06 revealed that 3,224 were differentially expressed genes (DEGs), 55 of which were related to reactive oxygen species metabolism, the Mitogen-activated protein kinase (MAPK) signaling pathway, and the meiosis pathway. Ten genes in the mitotic and meiotic pathways and another two genes associated with the “response to DNA damage stimulus” all had an upward expression, which were detected by qRT-PCR in ΔTs06 during cryopreservation. Gas chromatography–mass spectrometry (GC-MS) confirmed that the amounts of hexadecanoic acid and octadecadienoic acid were much more in ΔTs06 than in Ts06. In addition, using spectrophotometry, hydrogen peroxide (H2O2) was also present in greater quantities in ΔTs06 compared with those found in Ts06. Increased fatty acids metabolism could prevent damage to urediniospores in super-low temperatures, but oxidant species that involved H2O2 may destroy tube proteins of mitosis and meiosis, which could cause abnormal nuclear division and lead to multinucleation, which has a different genotype. Therefore, the multinuclear isolate is different from the wild-type isolate in terms of phenotype and genotype; this multinucleation phenomenon in urediniospores improves the pathogenesis and environmental fitness of M. larici-populina.

Stoichiometric flexibility and soil bacterial communities respond to nitrogen fertilization and neighbor competition at the early stage of primary succession

At the early stage of primary succession, there are deficient nutrient resources as well as competition stress among neighboring plants. Our aims were to elucidate the flexibility of tree seedlings’ stoichiometric relationships and their effects on soil microbial communities, and to determine the driving forces of species turnover during primary succession through the evaluation of carbon (C) : nitrogen (N) : phosphorus (P) stoichiometric relationships. We conducted an experiment testing N addition effect on two species from the early stage of primary succession, under intra- and interspecific competition conditions. Our results showed that higher values of δ15N-NO3− and δ13C were observed in Populus purdomii individuals than in Salix rehderiana after N application, which indicated a more efficient N uptake and water-use efficiency in P. purdomii plants. Furthermore, under N addition, the intraspecific competition of P. purdomii presented a higher urease activity, microbial biomass C (MBC), microbial N:P ratio (MBN:MBP), and phylogenetic diversity compared to the intraspecific competition of S. rehderiana. The results showed that P. purdomii seedlings influenced soil properties in a way that led to a positive feedback on their performance with an increasing N availability. In contrast, S. rehderiana seedlings influenced soil properties in a way that caused a negative feedback on their performance with increasing N. Such events can promote species turnover from Salix to Populus during succession. Additionally, DNA sequencing of soil bacterial communities showed differences in the composition of microbial communities in response to N fertilization and different competition patterns. Altogether, our results showed that plant, soil, and microbial community responses to N fertilization in a subalpine glacier forefield differed among tree species and competition patterns. This study brings new insight into mechanisms that drive species replacement and biogeochemical cycling during primary succession.

Ecoenzymatic stoichiometry and microbial nutrient limitations in rhizosphere soil along the Hailuogou Glacier forefield chronosequence

Mountain glaciers retreat at an increased rate under global warming, resulting in exposed barren surfaces for primary succession. Soil microbes are an important driver of ecosystem processes. Although variations in soil microbes after deglaciation have been studied extensively, the roles of rhizosphere soil microbes in the biogeochemistry cycle during primary succession are less understood. In this study, Populus purdomii was present throughout the 123-year chronosequence as a representative tree species. We therefore investigated variations in the rhizosphere enzyme activity, microbial community structure, and ecoenzymatic stoichiometry of P. purdomii along Hailuogou Glacier chronosequences. The objective was to determinechanges in rhizosphere enzyme activities and microbial communities, as well as the effects of nutrient limitation on rhizosphere microbes. According to the results, the enzyme activities and microbial group biomass in rhizosphere soil all showed a bimodal trend and were highest at the 43rd or 123rd year, and enzyme activity varied with succession time but not microbial community structure. The rhizosphere soil bacterial community was the dominant community during the 123-year chronosequence. Ecoenzymatic stoichiometry indicated nitrogen restrictions on microbial activity throughout primary succession, with early succession stages (5–15 years) showing greater carbon restriction than late succession stages. Moreover, redundancy and correlation analyses demonstrated that soil microbial phospholipid fatty acid biomass was an important factor for increases in enzyme activities and that enzyme activities in turn played important roles in carbon, nitrogen and phosphorus cycling in rhizosphere soil. Additionally, rhizosphere soil microbial development significantly affected soil organic carbon, total nitrogen and dissolved organic carbon accumulation. Overall, our study links the rhizosphere microbial community and activity to successional chronosequences, providing a deeper understanding of the dynamics of ecosystem succession.

Leaf Cuticle Can Contribute to Non-Host Resistance to Poplar Leaf Rust

The plant leaf cuticle is a chemically complex but largely waxy outer shell that limits water loss and also prevents some pathogens from gaining access to internal mesophyll. Rust fungi are obligate parasites, and most bypass the cuticle by thigmotropically locating stomata, growing through the stomatal openings, and then parasitizing mesophyll cells with haustoria. It is thought that even non-hosts of a given rust fungus do not resist until their mesophyll cells are contacted in this way. In other words, it is thought that the cuticle plays no role in non-host resistance. Here, we tested the hypothesis that poplar leaf cuticles might contribute to non-host resistance to rust fungi by chemically impeding the germination and growth of urediniosporelings of Melampsora larici-populina. Following an initial survey in China of the resistance of 36 genotypes of various species and interspecific hybrids of Populus to M. larici-populina, we selected three genotypes for the initial test of hypothesis: (1) A Populus purdomii genotype that is fully susceptible; (2) a Populus deltoides cv. ‘I-69′ that is incompletely resistant (i.e., a resistant host); and (3) a Populus tomentosa genotype that is a non-host to M. larici-populina. Urediniospores were assayed for germination in extracts of the cuticles of the three genotypes. Germination was most reduced by the P. tomentosa non-host cuticular extracts that also reduced the growth of germ tubes to 36 times less than that in controls or in the extract of the susceptible P. purdomii. Four cuticular components were identified as putative defense compounds given greater concentrations in P. tomentosa than in P. purdomii: Aucubin, hexakis(trimethylsilyl) ether, catechol, 7,9-Di-tert-buty l-1-oxaspiro (4,5) deca-6, 9-diene-2,8-dione and trifluoroacetamide. These four compounds were then tested, and they reduced urediniospore germination and uredinial density in inoculations of normally susceptible P. purdomii with Melampsora larici-populina. Thus, the cuticle of P. tomentosa can contribute to pre-haustorial, non-host resistance to M. larici-populina.

Mutual effects between Pinus armandii and broadleaf litter during mixed decomposition

Mixed-decomposition effects are commonly observed in natural and planted forests and affect nutrient cycling in a forest ecosystem. However, how one litter type affects the decomposition of another is still poorly understood. In this study, <em>Pinus armandii</em> litter was mixed with <em>Betula albosinensis</em>, <em>Catalpa fargesii</em>, <em>Populus purdomii</em>, <em>Eucommia ulmoides</em>, and <em>Acer tsinglingense</em> litter. The mixtures were placed in litterbags and buried in soil with consistent moisture for a 180-day indoor simulated decomposition experiment. The litterbags were periodically harvested during decomposition; the litter residues of different species were separated, and the biomass dynamics of each litter type were simulated. In addition, the soil sucrase, cellulase and polyphenol oxidase activities were also detected three times. The mutual effects of needle and broadleaf litter during mixed decomposition and the possible underlying mechanisms were investigated. The results indicated that (i) during the decomposition experiment, <em>P. armandii</em> needles significantly inhibited the decomposition of broadleaf litter in the first 3 months, while the broadleaf litter accelerated the decomposition of <em>P. armandii</em> needles in only approximately 40% of the cases. However, the inhibitory effects of needles on broadleaf litter decomposition subsequently exhibited significant weakening, while the accelerating effects of broadleaf litter were significantly enhanced. The effects of mixed decomposition on the activities of three enzymes can only partially explain the interactions between different litter types; (ii) the prediction by the decomposition model showed that most of the broadleaf litter types could continuously accelerate the decomposition of <em>P. armandii</em> needles throughout the mixed decomposition process, while the decomposition of broadleaf litter would be significantly inhibited at least in the short term. In general, four of the five broadleaf litter types (excluding <em>E. ulmoides</em>) could accelerate the early decomposition of <em>P. armandii</em> needles and consequently accelerate nutrient cycling in <em>P. armandii</em> pure forests. These species could be used for the transformation of pure <em>P. armandii</em> pure forests to mixed forests.

Increasing soil age drives shifts in plant-plant interactions from positive to negative and affects primary succession dynamics in a subalpine glacier forefield

The stress gradient hypothesis predicts that plant-plant interactions switch between facilitation (positive) and competition (negative) along environmental gradients, with facilitation being more common under high abiotic stress conditions relative to more moderate abiotic stress conditions. Our aim was to reveal, whether the interactions between Populus purdomii Rehder and Salix rehderiana Schneider switch from positive to negative during the early stages of primary succession in the Gongga Mountain glacier retreat region. We also investigated, whether soil age is a major driving factor for the transformation of interactions between neighboring plants. We analyzed differences between intraspecific interactions and interspecific interactions of Populus and Salix under 20- and 40-year-old soil conditions, including plant biomass accumulation and allocation, nutrient absorption and utilization, relative competition intensity, non-structural carbohydrates, foliar carbon and nitrogen isotope composition, mesophyll cell ultrastructure, soil microbial biomass and community structure, extracellular enzyme activities, and soil organic carbon (SOC), soil total nitrogen (TN), soil ammonium (NH4+-N), and soil nitrate (NO3−-N) contents. We found that P. purdomii and S. rehderiana growing under interspecific interactions had greater contents of aboveground dry matter, belowground dry matter and total dry matter compared to intraspecific interactions in 20-year-old soil. Furthermore, in 40-year-old soil conditions, the phospholipid fatty acid (PLFA) analysis showed that Populus and Salix exposed to interspecific interactions exhibited lower amounts of gram-positive bacteria, fungi (18,1 ω9c) and actinomycetes, and lower levels of total PLFAs than those growing under intraspecific interactions. The redundancy analysis (RDA) results demonstrated that soil N was the most important parameter contributing to the composition of microbial communities. In addition, the 15N stable isotope labeling method showed that Populus and Salix growing under interspecific interactions had higher foliage δ15N derived from NO3− (δ15N-NO3−) than those growing under intraspecific interactions in 20-year-old soil. In summary, our results demonstrated that Populus-Salix interactions exhibited positive effects on survival in 20-year-old soil. Conversely, under 40-year-old soil conditions, Populus-Salix interactions presented negative effects in relation to nutrients and elimination by neighboring plants. Moreover, soil age is a major driving factor for plant-plant interactions that shift from positive to negative with an increasing soil age in the Gongga Mountain glacier forefield. In all, our results support the stress gradient hypothesis. Our findings improve understanding of plant-plant interactions and plant-soil feedbacks during the early stages of soil development, and of the construction of vegetation communities.

First Report of Branch Cankers Caused by Sphaerulina musiva on Populus purdomii in the United States

First Report of Branch Cankers Caused by <i>Sphaerulina musiva</i> on <i>Populus purdomii</i> in the United States

Impacts of plant secondary metabolites from conifer litter on the decomposition of Populus purdomii litter

A decomposition experiment of Populus purdomii litter, a commonly used afforestation species in the Qinling Mountains, China, was conducted. Water extracts of five conifer litters were used to treat P. purdomii litter and its soil. The P. purdomii litter was incubated at room temperature (20–25 °C) and constant soil moisture for 6 months. During the decomposition period, the dynamics of litter mass and nutrient contents were detected to investigate the impacts of plant secondary metabolites. The results indicate that litter extracts of Pinus tabuliformis, Platycladus orientalis, Pinus armandii and Larix principis-rupprechtii significantly inhibited the overall nutrient release of P. purdomii litter, while the last three types of litter extracts simultaneously inhibited its decomposition. Conversely, the litter extracts of Picea asperata significantly accelerated the overall nutrient release of P. purdomii. Generally, most of the conifer species, whose litter released terpenoids , phenolics, steroids, and aliphatic acids, will inhibit the decomposition and/or nutrient release from P. purdomii litter. Their negative effects on the decomposers and soil enzymatic activities indicates that planting diversity should be lower when mixed planted with P. purdomii.

Reproductive investments driven by sex and altitude in sympatric Populus and Salix trees.

The sex ratios of co-existing Populus and Salix vary depending on altitude and species: 1:1 equlibrium sex ratios are observed at mid-altitude but skewed ones at high altitudes, where Populus shows male-biased and Salix female-biased sex ratios. However, the underlying ecological mechanisms are poorly known. Reproductive investments of Populus purdomii Rehd. and Salix magnifica Hemsl. were assessed at altitudes of 2000 and 2600 m in the Gongga Mountain by different metrics, including biomass, carbon (C), nitrogen (N) and phosphorus (P) concentrations and construction cost, and by estimating the payback time that combines energy gain and associated costs. Reproductive investment measured as C, N and P concentrations, and construction cost was higher in P. purdomii females at 2600 m. However, in S. magnifica, no difference was observed for biomass, C and N at 2600 m, but the investments for P and construction cost were even greater in males. The payback time showed no significant differences for the sexes at 2000 m, but it was shorter for P. purdomii males and S. magnifica females at 2600 m. We concluded that nutrient- and construction cost-based estimates of reproductive allocation can provide more informative insight into the cost of reproduction than does biomass, and together with the payback time they can supply an explanation for divergent sex ratios in Populus and Salix. Consequently, our results improve our understanding of the causes and consequences of sexual dimorphism in dioecious species.

Nitrogen-controlled intra- and interspecific competition between Populus purdomii and Salix rehderiana drive primary succession in the Gongga Mountain glacier retreat area.

In this study, intra- and interspecific competition were investigated in early successional Salix rehderiana Schneider and later-appearing Populus purdomii Rehder under non-fertilized (control) and nitrogen (N)-fertilized conditions in the Hailuogou glacier retreat area. Our aim was to discover whether N is a key factor in plant-plant competition and whether N drives the primary succession process in a glacier retreat area. We analyzed differences in responses to intra- and interspecific competition and N fertilization between P. purdomii and S. rehderiana, including parameters such as biomass accumulation, nutrient absorption, non-structural carbohydrates, photosynthetic capacity, hydrolysable amino acids and leaf ultrastructure. In the control treatments, S. rehderiana individuals subjected to interspecific competition benefited from the presence of P. purdomii plants, as indicated by higher levels of biomass accumulation, photosynthetic capacity, N absorption, amino acid contents and photosynthetic N-use efficiency. However, in the N-fertilized treatments, P. purdomii individuals exposed to interspecific competition benefited from the presence of S. rehderiana plants, as shown by a higher growth rate, enhanced carbon gain capacity, greater amino acid contents, and elevated water-use efficiency, whereas the growth of S. rehderiana was significantly reduced. Our results demonstrate that N plays a pivotal role in determining the asymmetric competition pattern among Salicaceae species during primary succession. We argue that the interactive effects of plant-plant competition and N availability are key mechanisms that drive primary succession in the Gongga Mountain glacier retreat area.

Biomonitoring trace metal contamination by seven sympatric alpine species in Eastern Tibetan Plateau

Biomonitoring permits determinations of trace metal contamination in remote areas like the high mountain ecosystems. In this study, six trace metals (Cd, Cr, Cu, Ni, Pb, and Zn) in seven sympatric alpine species (five tree species: Salix rehderiana, Populus purdomii, Betula albosinensis, Abies fabri, Picea brachytyla, and two dominant mosses: Pleurozium schreberi, Papillaria crocea) at the Hailuogou Glacier foreland, Eastern Tibetan Plateau, were investigated to monitor their contamination. The concentrations of trace metals and Pb isotopic ratios (206Pb/207Pb and 208Pb/206Pb) in leaves/needles, twigs, bark, roots, and mosses were determined, and the biological factors and enrichment factors were calculated. The concentrations of Cd, Cr, Pb, and Zn in mosses were significantly higher than those in tree tissues and normal plants indicating the exogenous sources. The accumulation of trace metals (except Cd and Zn) was relatively higher in the tree roots, whereas their enrichments were significant in the leaves/needles and bark. According to biological factors, enrichment factors, and factor analysis, Cd, Pb, and Zn in trees and mosses were markedly impacted by anthropogenic emissions, whereas Cr, Cu, and Ni in trees were mainly from root adsorption from soils. The Pb isotopic compositions identified the anthropogenic Pb mainly from mining and smelting, coal combustion, and vehicle exhausts. The results indicated that mosses were still priority indicator of trace metal contamination from atmospheric deposition, and the leaves and bark of S. rehderiana, P. purdomii, and B. albosinensis were the better alternatives to monitor the atmospheric contamination of trace metals in the alpine ecosystem.

Contrasting responses in the growth and energy utilization properties of sympatric Populus and Salix to different altitudes: implications for sexual dimorphism in Salicaceae.

An interesting ecological and evolutionary puzzle arises from the observations of male-biased sex ratios in genus Populus, whereas in the taxonomically related Salix, females are generally more dominant. In the present study, we combined results from a field investigation into the sex ratios of the Salicaceous species along an altitudinal gradient on Gongga Mountain, and a pot experiment by monitoring growth and energy utilization properties to elucidate the mechanisms governing sexual dimorphism. At middle altitudes 2000 and 2300 m, the sex ratios were consistent with a 1:1 equilibrium in sympatric Populus purdomii and Salix magnifica. However, at the lower and higher ends of the altitudinal gradient, skewed sex ratios were observed. For example, the male:female ratios were 1.33 and 2.36 in P. purdomii at 1700 and 2600 m respectively; for S. magnifica the ratio was 0.62 at 2600 m. At 2300 m, the pot-grown seedlings of both species exhibited the highest biomass accumulation and total leaf area, simultaneously with the balanced sex ratios in the field. At 3300 m, the specific leaf area in male P. purdomii was 23.9% higher than that of females, which may be the morphological cause for the observed 19.3% higher nitrogen allocation to Rubisco, and 20.6% lower allocation to cell walls. As such, male P. purdomii showed a 32.9% higher foliar photosynthetic capacity, concomitant with a 12.0% lower construction cost. These properties resulted in higher photosynthetic nitrogen- and energy-use efficiencies, and shorter payback time (24.4 vs 40.1 days), the time span that a leaf must photosynthesize to amortize the carbon investment. Our results thus suggested that male P. purdomii evolved a quicker energy-return strategy. Consequently, these superior energy gain-cost related traits and the higher total leaf area contributed to the higher growth rate and tolerance in stress-prone environments, which might, in part, shed new light on the male-biased sex ratios in Populus. However, no significant sexual difference was observed in S. magnifica for all the above parameters, thereby implying that the female-biased sex ratios in Salix cannot be explained in terms of the energy-use properties studied here.

Assessment of arbuscular mycorrhizal fungi status and heavy metal accumulation characteristics of tree species in a lead–zinc mine area: potential applications for phytoremediation

To select suitable tree species associated with arbuscular mycorrhizal fungi (AMF) for phytoremediation of heavy metal (HM) contaminated area, we measured the AMF status and heavy metal accumulation in plant tissues in a lead-zinc mine area, Northwest China. All 15 tree species were colonized by AM fungi in our investigation. The mycorrhizal frequency (F%), mycorrhizal colonization intensity (M%) and spore density (SP) reduced concomitantly with increasing Pb and Zn levels; however, positive correlations were found between arbuscule density (A%) and soil total/DTPA-extractable Pb concentrations. The average concentrations of Pb, Zn, Cu and Cd in plant samples were 168.21, 96.61, 41.06, and 0.79mg/kg, respectively. Populus purdomii Rehd. accumulated the highest concentrations of Zn (432.08mg/kg) and Cu (140.85mg/kg) in its leaves. Considerable amount of Pb (712.37mg/kg) and Cd (3.86mg/kg) were concentrated in the roots of Robinia pseudoacacia Linn. and Populus simonii Carr., respectively. Plants developed different strategies to survive in HM stress environment: translocating more essential metals (Zn and Cu) into the aerial parts, while retaining more toxic heavy metals (Pb and Cd) in the roots to protect the above-ground parts from damage. According to the translocation factor (TF), bioconcentration factor (BCF), growth rate and biomass production, five tree species (Ailanthus altissima (Mill.) Swingle, Cotinus coggygria Scop., P. simonii, P. purdomii, and R. pseudoacacia) were considered to be the most suitable candidates for phytoextraction and/or phytostabilization purposes. Redundancy analysis (RDA) showed that the efficiency of phytoremediation was enhanced by AM symbioses, and soil pH, Pb, Zn, and Cd levels were the main factors influencing the HM accumulation characteristics of plants.

Soil carbon, nitrogen and microbial biomass dynamics of subalpine Abies fabri forest in Gongga Mountain, Southwest China

Soil organic carbon (SOC), soil microbial biomass C, soil total nitrogen (N), NH4+-N and NO3--N dynamics were measured in subalpine Abies fabri forest at different growth phases which include the mature forest (MF, 3100 m a.s.l.), the middle age forest (MAF, 2998 m a.s.l.) and the succession forest of mixed Abies fabri and Populus purdomii Rehd(SF, 2947 m a.s.l.) of Gongga Mountain, Southwest China. SOC concentrations decreased significantly with increasing depth and at a given depth (0-10 cm, 10-20 cm, 20-40 cm, 40-60 cm and 60-100 cm), SOC was greater in MF than that in MAF and SF. The average SOC concentration in MF (50.99 g kg-1) was almost six times than that in MAF (7.97 g kg-1) and SF (6.25 g kg-1). Soil microbial biomass C fluctuated in time and was higher in August than that in June and October under all A. fabri forests. Soil NH4+ -N, NO3- -N and soil available N (NH4+ -N + NO3- -N) were higher in MF than those in MAF and in SF. The average soil available N concentrations were 21.61, 12.57 and 10.91 mg kg-1under MF, MAF and SF, respectively. Key words: Soil organic carbon, soil nitrogen, Abies fabri forest, Gongga Mountain.