Poplar Branches Research Articles

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes.

Valsa sordida and Botryosphaeria dothidea are two crucial necrotrophic fungal pathogens that damage many plant hosts, particularly species in the genus Populus. These two fungal pathogens occur mainly in poplar branches, stems, and twigs, causing classic symptoms such as canker lesions, canopy dieback, and wilting. Pathogen inoculation is the most efficient pathway to study the mechanism of plant disease. Besides the canker lesions around the inoculation sites on the stems, a novel developmental phenomenon, copious adventitious roots (ARs) with bright red color, were observed in poplar species after stem canker pathogen inoculations. In this study, we described the method for inducing ARs using fungal pathogens in poplar trees. The crucial step of this method is the pathogen inoculation after (phloem or epidermis) girdling manipulation. The second crucial step is the application of the moisturizing material. Compared to the moisturizing manipulation with Parafilm, wrapping the inoculated sites with household polyethylene (PE) plastic wrap can produce colorful, numerous, and robust ARs in 20 days after girdling-inoculation. Finally, white ARs sprouted from the inoculated rings in the poplar stems after shading treatment (wrapping the stems with aluminum foil). This method introduces a novel experimental system for studying root development and morphogenesis, which is crucial for understanding the biology of root development, morphogenesis, and response under disease stress. Furthermore, when combined with shading treatment, this study can provide a convenient experimental system for investigating light response-related processes, for example, the biosynthesis of flavonoids, anthocyanins, or other related metabolites, and genes or transcription factors involved in these processes.

Biotemplate synthesis of graphitic carbon-doped ZnO tube bundles rich in oxygen vacancies for dual-sensing of NO2/H2S at different temperatures

How to fabricate one chemiresistive sensor for highly dual-selective detection of hazardous NO2 and H2S gases is a challenge owing to the fact that the great majority of gas sensors were designed to monitor only a given harmful gas. For the aim of portable practicality and internet of things, based on green and sustainable concept, natural poplar branches were utilized as template and simply soaked in zinc nitrate solution. Subsequently, the immersed biotemplates underwent air calcination to controllably reproduce two biomorphic ZnO-based materials. Among, the hierarchical tubes (GC/ZnO) prepared by calcination at 450 °C are interconnected by 5.68 wt% in-situ graphitic carbon (GC) and uniform nanoparticles, which possess small mesopores, large specific surface area and massive oxygen vacancies. Such distinctive microstructure not only facilitates rapid gas diffusion into sensing layer and modulates the state of adsorbed oxygen species, but also exposes more active sensing units and promotes surface chemical reactions, thus realizing a portable gas sensor for highly sensing and dual-selective detection of NO2 and H2S. At lower 92 °C or 133 °C, the GC/ZnO sensor exhibits response values of 203 and 65.5 for 10 ppm NO2 or H2S gases, respectively. Meanwhile, it possesses reversible response-recovery, low detection limit, good moisture tolerance and stability during 60 days. In addition, the temperature-controlled dual-response mechanism is also analyzed.

Mechanical properties of poplar (NL-3412) branches

ABSTRACT The mechanical properties of poplar (NL-3412) branches are the basis for designing and optimizing the working parameters of branch pruning and crushing machinery. In this study, the tensile, compression, bending and shear properties of poplar branches were investigated by utilizing a precision microcomputer-controlled electronic universal testing machine. The test results suggested that the elastic constitutive relationship of the stress–strain curves of poplar branches under longitudinal compression, transverse compression, and bending conformed to Hooke's law, and longitudinal tensile and shear exhibited plastic damage; the anti-failure strength, anti-failure elastic modulus, and external work of poplar branches under longitudinal compression were greater than those under transverse compression. The analysis results indicated that the correlation function between the anti-failure strength and the external force work of poplar branches is positively correlated, and the external force work required to destroy poplar branches can be estimated by the established regression mathematical model. The results of the study can provide important data support for the optimal design of poplar branch pruning and shredding machinery and related simulation experimental research. HIGHLIGHTS The basic parameters of mechanical properties of poplar branches were determined. The variation law of stress–strain curve of poplar branch mechanical test was proved. The mathematical model between the strength and external force work was established. Provide reference for the optimal design of branch pruning and crushing machinery. Provide data support for the simulation cutting test of poplar branch.

Linear Cutting Performance Tests and Parameter Optimization of Poplar Branches Based on RSM and NSGA-II

To reduce the cutting force and cutting energy consumption during the operation of tree-climbing pruning machines for poplar trees, a linear cutting test bench device for branches was designed according to the growth characteristics of poplar branches and pruning forestry requirements in this study. Firstly, the cutting mechanical analysis of poplar branches was carried out to explore the significance parameters affecting the cutting force, and then the cutting performance test and parameter optimization of poplar branches was carried out through the response surface method (RSM). The test results indicated that cutting speed, tool edge angle and tool back angle had significant effects on the ultimate shear stress, cutting power consumption per unit area, and the branch damage rate of poplar branches, and the established regression equation demonstrated high goodness of fit. Meanwhile, a second-order regression mathematical model was developed between ultimate shear stress, cutting the power consumption per unit area of the cut and the branch damage rate, and the significance parameter. The non-dominated Sorting Genetic Algorithm II (NSGA-II) was used for multi-objective optimization computation to obtain the optimal combination of cutting parameters as 3.02 m/s for cutting speed, 15° for tool edge angle, and 3° for tool back angle. In this case, the ultimate shear stress, cutting power consumption per unit area, and branch damage rate of poplar branches were small, which were 346.63 kPa, 9.35 mJ/mm2, and 12.36%, respectively. Through the test verification, it can be seen that the relative error between the verification test and the predicted value of model was less than 7%. Moreover, under a cutting tool edge angle of 15°, the ultimate shear stress, cutting power consumption per unit area, and branch damage rate were, respectively, reduced by 17.29%, 14.98%, and 34.21% compared with those under a cutting tool edge angle of 20°, which verifies the validity and reliability of the test results and the research method. This study can provide data support and reference for the research and development of energy-efficient poplar tree-climbing pruning equipment and related branch-cutting performance tests.

Biotemplate synthesis of single crystal MoO3 hierarchical tubules assembled from nano-rectangles for conductometric sensing of diisopropylamine vapor

The development of metal oxide-based gas sensors with high sensing to carcinogenic organoamines has recently attracted much attention. However, there is no related report on detecting diisopropylamine (DIPA). Herein, renewable poplar branches were selected as bio-template and immersed in ammonium molybdate solution. Subsequently, the above precursors were calcined in air to successfully synthesize two types of MoO3 nano-rectangles with different sizes. Amongst, biomorphic MoO3-500 tubules cross-linked by relatively small nano-rectangles were simply replicated by calcining precursors at 500 °C, which have multistage pores and single-crystal nature. Such structure characteristics can facilitate the fast diffusion of target gas, and increase the conductivity, active sites, as well as content of adsorbed oxygen species in sensing material. Especially, under the synergism of surface unsaturated Mo6+ catalysis, we realized the conductometric detection of DIPA vapor by metal oxide-based sensor for the first time. At 170 °C, MoO3-500 sensor shows high response value (244) and fast response speed (4 s) to 100 ppm DIPA vapor. Meanwhile, it still presents low detection limit, good selectivity and stability. Therefore, MoO3-500 tubules are expected to be a promising material for accurately detecting trace DIPA vapor.

Light-Dependence of Formate (C1) and Acetate (C2) Transport and Oxidation in Poplar Trees.

Although apparent light inhibition of leaf day respiration is a widespread reported phenomenon, the mechanisms involved, including utilization of alternate respiratory pathways and substrates and light inhibition of TCA cycle enzymes are under active investigation. Recently, acetate fermentation was highlighted as a key drought survival strategy mediated through protein acetylation and jasmonate signaling. Here, we evaluate the light-dependence of acetate transport and assimilation in Populus trichocarpa trees using the dynamic xylem solution injection (DXSI) method developed here for continuous studies of C1 and C2 organic acid transport and light-dependent metabolism. Over 7 days, 1.0 L of [13C]formate and [13C2]acetate solutions were delivered to the stem base of 2-year old potted poplar trees, while continuous diurnal observations were made in the canopy of CO2, H2O, and isoprene gas exchange together with δ13CO2. Stem base injection of 10 mM [13C2]acetate induced an overall pattern of canopy branch headspace 13CO2 enrichment (δ13CO2 +27‰) with a diurnal structure in δ13CO2 reaching a mid-day minimum followed by a maximum shortly after darkening where δ13CO2 values rapidly increased up to +12‰. In contrast, 50 mM injections of [13C]formate were required to reach similar δ13CO2 enrichment levels in the canopy with δ13CO2 following diurnal patterns of transpiration. Illuminated leaves of detached poplar branches pretreated with 10 mM [13C2]acetate showed lower δ13CO2 (+20‰) compared to leaves treated with 10 mM [13C]formate (+320‰), the opposite pattern observed at the whole plant scale. Following dark/light cycles at the leaf-scale, rapid, strong, and reversible enhancements in headspace δ13CO2 by up to +60‰ were observed in [13C2]acetate-treated leaves which showed enhanced dihydrojasmonic acid and TCA cycle intermediate concentrations. The results are consistent with acetate in the transpiration stream as an effective activator of the jasmonate signaling pathway and respiratory substrate. The shorter lifetime of formate relative to acetate in the transpiration stream suggests rapid formate oxidation to CO2 during transport to the canopy. In contrast, acetate is efficiently transported to the canopy where an increased allocation towards mitochondrial dark respiration occurs at night. The results highlight the potential for an effective integration of acetate into glyoxylate and TCA cycles and the light-inhibition of citrate synthase as a potential regulatory mechanism controlling the diurnal allocation of acetate between anabolic and catabolic processes.

Biotemplate-derived mesoporous Cr2O3 tube bundles for highly sensitive and selective detection of trace acetone at low temperature

The development of Cr2O3-based acetone sensor is challenging due to no report on its high sensing ability. To this end, we simply dipped poplar branches into chromic chloride solution to obtain immersed precursors, which were then calcined in air to controllably synthesize two biomorphic Cr2O3 tube bundles. Amongst, the hierarchical tube structure (Cr2O3-500) calcined at 500 °C is replicated from small-size nanoparticles, so that it has uniform mesopore distribution and large specific surface area. Such microstructural features not only favor the rapid accessibility of gas molecules to sensing layer, but also provide an effective platform of more active sites for facilitating surface adsorption and chemical reaction. Especially, under the synergism of few non-stoichiometric CrO3-x species, Cr2O3-based sensor achieves highly sensitive and selective detection to trace acetone gas for the first time. At 133 °C, Cr2O3-500 exhibits high response value (S = 110.1) to 100 ppm acetone gas and low actual detection limit (10 ppb, S = 1.3). Simultaneously, it also has rapid response-recovery characteristics, good stability and moisture resistance. In addition, the sensing mechanism is discussed in details.

Influence of biomass type on its characteristics of convective heating and dehydration

The paper presents the results of experimental studies of the drying processes of forest biomass, which is promising for energy purposes, - branches of various types of wood: birch, linden, poplar. The experiments were carried out on an installation that provides a low level of error (no more than 8.5%) for measuring the main significant parameters and characteristics of the dehumidification process. According to the results of the experiments, the integral characteristics of the greatest interest (times of complete dehydration) of the drying process have been established. Based on the results of the experiments, a non-monotonic change in the rates of moisture removal was established (the values of Weva increase and decrease with time) at a low-temperature heating mode (at Tg<373 K). At the same time, under conditions of relatively high ambient temperatures (Tg≥373 K), such a change in Weva does not occur. The authors formulated a hypothesis explaining the physical mechanism of such a dependence of the mass rate of moisture removal (Weva) on time. An energy analysis of the drying efficiency of three types of biomass has been carried out. It has been shown that branches of birch, poplar and linden are most efficiently dried in conditions of relatively low temperatures (333≤Tg≤353 K). At the same time, it was found that for birch and poplar branches, an increase in the ambient temperature leads to a decrease in the efficiency of the drying process of woody biomass.

Combination of hydrothermal and chemi-mechanical pretreatments to enhance enzymatic hydrolysis of poplar branches and insights on cellulase adsorption

An integration of different pretreatments is important to overcome recalcitrance and realize efficient bioconversion of lignocellulosic biomass. This study aims at the effects of combination of hydrothermal pretreatment and different chemi-mechanical pretreatments on enzymatic hydrolysis, and understanding the enzymes adsorption mechanism. The combination of hydrothermal and chemi-mechanical pretreatments effectively improved the enzymatic hydrolysis of poplar substrates, in which the enzymatic hydrolysis of substrates pretreated by hydrothermal pretreatment + Fenton pretreatment + mechanical refining (HFM) was the highest (92.39% of glucose conversion yield, and 20.88 g/L of glucose concentration). The substrates’ main characteristics were obviously changed after combined pretreatments, such as swelling ability and specific surface area of substrates were increased. The Langmuir adsorption model (R2 > 0.98) and pseudo second-order adsorption kinetic model (R2≈1) were well suitable to describe the adsorption of enzymes on substrates, meanwhile the adsorption mechanism was summarized.

Are Methanol-Derived Foliar Methyl Acetate Emissions a Tracer of Acetate-Mediated Drought Survival in Plants?

Upregulation of acetate fermentation in plants has recently been described as an evolutionarily conserved drought survival strategy, with the amount of acetate produced directly correlating to survival. However, destructive measurements are required to evaluate acetate-linked drought responses, limiting the temporal and spatial scales that can be studied. Here, 13C-labeling studies with poplar (Populus trichocarpa) branches confirmed that methyl acetate is produced in plants from the acetate-linked acetylation of methanol. Methyl acetate emissions from detached leaves were strongly stimulated during desiccation, with total emissions decreasing with the leaf developmental stage. In addition, diurnal methyl acetate emissions from whole physiologically active poplar branches increased as a function of temperature, and light-dark transitions resulted in significant emission bursts lasting several hours. During experimental drought treatments of potted poplar saplings, light-dark methyl acetate emission bursts were eliminated while strong enhancements in methyl acetate emissions lasting > 6 days were observed with their initiation coinciding with the suppression of transpiration and photosynthesis. The results suggest that methyl acetate emissions represent a novel non-invasive tracer of acetate-mediated temperature and drought survival response in plants. The findings may have important implications for the future understanding of acetate-mediated drought responses to transcription, cellular metabolism, and hormone signaling, as well as its associated changes in carbon cycling and water use from individual plants to whole ecosystems.

Designing a new wood-composite material made from logging waste

This study has confirmed the possibilities of using logging waste for the production of wood-composite material. The raw material for its manufacturing is the wood weaving made by flattening branches. An experimental study into the physical, mechanical, and technological properties of the wood-composite material made from the poplar branches has made it possible to determine its rational structure and the type of a binder. It has been established that ensuring the flat shape of the material requires that it should be formed of three mutually perpendicular layers. It is advisable to use urea formaldehyde resin as a binder for plates that are not exposed to the impact and static loads and are used indoors. When the material is to be applied under conditions of elevated loads and in places with high humidity, phenol-formaldehyde resin should be used. The rational pressing mode parameters have been defined: temperature, t =180 °C; pressure, p =8 MPa; holding time, τ=7 min., at which one can achieve a minimum deformation of plates after pressing. It has been proven that under these schedule parameters the holding time for the new wood-composite material to achieve the shape stabilization is also minimal; it is seven days. It has been determined that the new wood-composite material possesses the physical and mechanical properties similar to those of the OSB panels

Improving enzymatic hydrolysis of mechanically refined poplar branches with assistance of hydrothermal and Fenton pretreatment

The combination of different pretreatment methods can effectively overcome recalcitrance of lignocellulosic biomass to ensure its highly efficient conversion into bio-based products. In this study, the combined pretreatments of chemical methods (hydrothermal treatment and Fenton treatment) with mechanical refining were used to improve the enzymatic hydrolysis efficiency of poplar branches. The results indicated that hydrothermal pretreatment and Fenton pretreatment can effectively improve the enzymatic hydrolysis of poplar substrates, e.g., the maximum glucose conversion yield and glucose concentration reached 92.4% and 20.8 g/L, respectively. The pre-hydrolysates contained some valuable components such as monosaccharides, oligosaccharides, acetic acid, furfural, and hydroxymethylfurfural. The main characteristics (specific surface area, water retention value, fines content, and surface lignin concentration) of poplar substrates were obviously changed by the combined pretreatment, which benefit the enzymatic hydrolysis.

Real-time measurements of black carbon and other pollutant emissions from residential biofuel stoves in rural China

Biofuel stoves are an important source of black carbon (BC) emissions, which have adverse effects on the environment and human health, especially in rural areas. However, there have been only limited studies of BC emissions from residential biofuel stoves based on real-time measurements. In this study, a photo-acoustic extinctiometer (PAX)-based real-time measurement system was employed to monitor the emission characteristics of corncobs, corn stalks, cotton stalks and poplar branches in simple or improved stoves (with a total of 16 units) in Hebei Province, China. The real-time and phased emissions of BC, fine particulate matter (PM2.5), carbon monoxide (CO) and carbon dioxide (CO2) were assessed, and the effects of stove type and fuel on emissions were analyzed. Under the same conditions, polar branches were associated with the highest BC emission factors (EFs) of up to 2.64 ± 0.42 g kg−1, while the EFs for improved stoves were higher than those for simple stoves. During the ignition phase, BC emissions were found to be low, while the later addition of fuel dramatically increased emissions, followed by a gradual decrease until the next fuel addition. The phased results show that the flaming phase had the highest BC emission rate, the fuel addition phase was associated with the highest BC EF. The BC emission rates and EFs for the ignition, fuel addition, flaming and smoldering phases ranged from 0.0014–0.014, 0.11–6.32, 0.18–2.24 and 0.03–0.32 mg s−1, and from 0.04–0.18, 0.38–9.53, 0.45–3.55 and 0.12–1.01 g kg−1, respectively. This study assessed the BC emissions from residential biofuel stoves using a larger sample size than in prior work. The results increase our understanding of the BC emissions process, which is helpful in terms of improving the accuracy of BC EF estimations. The real-time measurement process described herein is also expected to provide new approaches to minimizing BC emissions.

Importance of adding woodchips to local amendments for improving soil health and increasing yield in severely degraded soils of Northern China

Wood chips, together with crop straw and manure, were demonstrated as feasible amendments for improving overall soil health and enhancing plant growth in severely desertified areas in Northern China in our previous studies. However, the effects of their mixtures are unclear. In this study, we evaluated the effect of three different amendment materials, when used alone and in combination, on physical, chemical, and biological properties of desertified soil and on the associated growth of alfalfa in Ningxia, Northern China. Four replicate microcosms were set up containing sandy soil mixed with 3% (w/w) of each of the following treatments: cow manure (CM), corn straw (CS), and poplar branches (PB) and their mixtures CS-PB, CM-PB, CM-CS, and CM-CS-PB. Measurements were conducted 7 and 15 months after the start of the experiment. Results showed, as a whole, treatment CS-PB improved soil physical properties more than other amendments during the whole experimental period, compared with control. However, the best establishment of alfalfa in the first year and best growth in the second year occurred in the CM-CS-PB treatment. Moreover, available N and urease were the most important driving factors affecting alfalfa growth during the whole experimental period, as shown by redundancy analysis. However, interestingly, the CM-CS-PB also functioned better in maintaining N supply and accelerating microbial activity than the other amendments. Therefore, we concluded that wood chips have a unique advantage in improving overall soil health and alfalfa growth when combined with straw and manure as new amendments.

Comparison of microbial community structures in soils with woody organic amendments and soils with traditional local organic amendments in Ningxia of Northern China.

BackgroundAddition of organic amendments has been commonly adopted as a means to restore degraded soils globally. More recently, the use of woody organic amendments has been recognized as a viable method of capturing and retaining water and restoring degraded and desertified soil, especially in semi-arid regions. However, the impacts of woody amendments on soil microbial community structure, versus other traditional organic supplements is less understood.MethodsThree locally available natural organic materials of different qualities, i.e., cow manure (CM), corn straw (CS), and chipped poplar branches (PB) were selected as treatments in Ningxia, Northern China and compared with control soils. Four microcosms served as replicates for each treatment. All treatments contained desertified soil; treatments with amendments were mixed with 3% (w/w) of one of the above organic materials. After 7 and 15 months from the start of the experiment, soil samples were analyzed for chemical and physical properties, along with biological properties, which included microbial α-diversity, community structure, and relative abundance of microbial phyla.ResultsBoth bacterial and fungal α-diversity indices were weakly affected by amendments throughout the experimental period. All amendments yielded different microbial community compositions than the Control soils. The microbial community composition in the CS and PB treatments also were different from the CM treatment. After 15 months of the experiment, CS and PB exhibited similar microbial community composition, which was consistent with their similar soil physical and chemical properties. Moreover, CS and PB also appeared to exert similar effects on the abundance of some microbial taxa, and both of these treatments yield different abundances of microbial taxa than the CM treatment.ConclusionNew local organic amendment with PB tended to affect the microbial community in a similar way to the traditional local organic amendment with CS, but different from the most traditional local organic amendment with CM in Ningxia, Northern China. Moreover, the high C/N-sensitive, and lignin and cellulose decompose-related microbial phyla increased in CS and PB have benefits in decomposing those incorporated organic materials and improving soil properties. Therefore, we recommend that PB should also be considered as a viable soil organic amendment for future not in Ningxia, but also in other places.

The HrpW protein of Lonsdalea quercina N‐5‐1 has pectate lyase activity and is required for full bacterial virulence

Lonsdalea quercina N-5-1 is a bacterial pathogen that causes poplar bark cankers. It has been isolated from the branch of Populus × euramericana cv. "74/76" in Henan, China. Previous studies have revealed that the Type III secretion system (T3SS) acts as an essential pathogenic factor in L. quercina N-5-1. HrpW is a putative effector of T3SS in strain N-5-1, which has a typical harpin domain at the amino terminal and a pectate lyase (Pel) domain at its carboxyl terminal. Genetic evidence had shown that, compared to the wild-type and the complementary strain, the hrpW mutation causes a small but significant reduction in virulence when inoculated on the poplar branches. The amino terminal domain of HrpW was found to trigger tobacco hypersensitive response, but the carboxyl terminal domain of HrpW was not. Unlike most HrpW homologs in other bacteria, the carboxyl terminal domain of HrpW of strain N-5-1 exhibited detectable pectate lyase activity. Site-direction mutations (W104A, W171M) further demonstrated that two tryptophan residues were essential to its pectate lyase activity. The results of the present work suggest that HrpW in L. quercina N-5-1 possesses pectate lyase activity and acts as a nonessential but important pathogenic factor in poplar bark canker disease.

Chipping machines: disc and drum energy requirements

Air pollution and fossil fuel reserves exhaustion are increasing the importance of the biomass-derived products, in particular wood, as source of clean and renewable energy for the production of electricity or steam. In order to improve the global efficiency and the entire production chain, we have to evaluate the energetic aspects linked to the process of transformation, handling and transport of these materials. This paper reports results on a comparison between two chippers of similar size using different cutting technology: disc and drum tool respectively. During trials, fuel consumption, PTO torque and speed, processing time and weight of processed material were recorded. Power demand, fuel consumption, specific energy and productivity were computed. The machine was fed with four different feedstock types (chestnut logs, poplar logs, poplar branches, poplar sawmill residues). 15 repetitions for each combination of feedstock-tool were carried out. The results of this study show that the disc tool requires, depending on the processed material, from 12 to 18% less fuel per unit of material processed than the drum tool, and consequently, from 12 to 16% less specific energy. In particular, the highest difference between tools was found in branches processing whereas the smallest was in poplar logs. Furthermore the results of the investigation indicate, that, in testing conditions, the productivity of drum tool is higher (8%) than disc tool.

Does freezing and dynamic flexing of frozen branches impact the cavitation resistance of Malus domestica and the Populus clone Walker?

Frost damage to the xylem conduits of trees is a phenomenon of eco-physiological importance. It is often documented in terms of the percentage loss of conductivity (PLC), an indicator of air filling of the conduits. However, trees that refill their conduits in spring could be impacted more by damage to the conduits that reduce cavitation resistance, making them more susceptible to future drought events. We investigated whether ice formation, dynamic flexing of frozen branches or freeze-thaw events could reduce the cavitation resistance (cause "frost fatigue") in first-year shoots of apple (Malus domestica) and clonal hybrid cottonwood (Walker). Frost fatigue was measured in terms of P50 (the negative xylem pressure required to cause a 50 % loss of conductivity). All treatment groups showed significant frost fatigue, with the exception of the pre-flushed, constantly frozen poplar branches. The P50 following freeze treatments was approximately 50 % of the pre-freeze values. The effect tended to be greater in freeze-thawed branches. Dynamic bending of the branches had no effect on either PLC or P50. In three out of four cases, there was a significant correlation between P50 and PLC. Frost fatigue occurred in both apple and poplar, two unrelated species with different drought and frost tolerances, suggesting that it may be a widespread phenomenon that could impact the ecophysiology of temperate forests.

Effects of jasmonic acid, branching and girdling on carbon and nitrogen transport in poplar

• Here, we examined the impact of jasmonate (JA) treatment, branching and phloem girdling on ¹³C and ¹⁵N import, invertase activity and polyphenol accumulation in juvenile tissues of unbranched and branched hybrid poplar saplings (Populus nigra × P. deltoides). • The import of ¹³C to juvenile tissues was positively correlated with invertase activity at the treatment site and enhanced by JA. Both invertase activity and ¹³C import were greater in shorter, younger branches and smaller, younger leaves. By contrast, JA treatments, branching and girdling had little or no impact on ¹⁵N import. • In poplar saplings with multiple lateral branches, we observed almost no ¹³C movement from subtending source leaves into lateral branches above them, with or without JA treatment. The presence of potentially competing branches, treated with JA or not, girdled or not, had no impact on carbohydrate (CHO) import or polyphenol accumulation in target branches. • We conclude that poplar branches comprise modules that are relatively independent from each other and from the stem below in terms of CHO movement, carbon-based defence production and response to elicitors. By contrast, branches are closely linked modules in terms of nitrogen movement. This should produce trees that are highly heterogeneous in quality for herbivores.

Microfibril Angle Distribution of Poplar Tension Wood

Tension wood of poplar (Populus nigra) branches was studied by lightand electron microscopy. The characteristic features of tension wood such as wider growth rings, reduced vessel density and higher gross density were confirmed by our results. Based on a novel combination of transmission electron microscopy (TEM) imaging and image analysis, involving Fourier transformation, the orientation of cellulose microfibrils in the S2- and G-layer was determined. Within the G-layer microfibril angle (MFA) was parallel to the growth axis (0°). However, in the S2 it was 13° in tension wood fibres and 4° in normal wood fibres. With the exception of the relatively low fibril angle in the S2 of tension wood fibres (13°) the results are in good agreement with those of the literature.