Stichopus cf. horrens is an emerging sea cucumber species in tropical aquaculture. However, stocking density must be optimized. This study investigated the effects of stocking density (278 ind. m−2, 556 ind. m−2, and 1111 ind. m−2) and the presence or absence of polyvinyl chloride (PVC) pipes as shelters on the growth performance and survival of juveniles (0.4 to 1.0 cm) in the field over 30 days. The activity pattern, sheltering behavior, and aggregation density of juveniles (1.5 to 2.0 cm) at different stocking densities (144 ind. m−2, 289 ind. m−2, and 578 ind. m−2) were further evaluated in the laboratory for 15 days. Results revealed negative density-dependent effects on the absolute growth rate (AGR) and the coefficient of variation in length among the juveniles. The presence of PVC pipes did not significantly improve AGR of juveniles in all density treatments by day 30. However, survival rates were higher in sheltered treatments compared to the non-sheltered. During inactive hours, aggregations of juveniles were observed on the undersides of the PVC pipes, with high-density aggregations prevalent only at medium and high stocking densities, but not at low stocking densities. The direct physical contact during these aggregations likely exacerbated the effects of crowding and increased the risk of exposure to the cytotoxic chemicals produced by conspecifics. Designing shelter models that reduce the probability of encounter during shelter-seeking, while enhancing epiphytic growth and grazing efficiency, is crucial for mitigating the negative effects of density on this high-value sea cucumber species.
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