Mount Merbabu National Park has the potential for abundant biodiversity in natural forest vegetation, including flora and fauna. The purpose of this study was to explore and identify fungi in Mount Merbabu National Park on Selo Climbing Lane, Boyolali, Central Java. The research was conducted in January until April 2020 on Selo Climbing Lane, Boyolali. This research was exploratory by using Cruise Methods, which explored the hiking trail at 1800-2571 meters altitude. Sampling was carried out every time the fungi were identified, and it was done when the same fungi were found. Identification was made by recording morphological characteristics, habitus, and the substrate through qualitative descriptive. The results showed that the 52 identified species of fungi belonged to the 26 families of fungi. The most common fungi are the Polyporaceae family, which consists of 5 species, namely: Trametes versicolor, Trametes polyzona, Polyporus sp, Polyporus versicolor dan Ganoderma applanatum.