Polyhedra Of Nuclear Polyhedrosis Virus Research Articles

Adsorption of polyhedra of a nuclear polyhedrosis virus of Heliothis armigera in two different types of soils

Tests on the quantitative adsorption of polyhedra of nuclear polyhedrosis virus of Heliothis armigera to black and red soil showed an increase in the adsorption of polyhedra with an increase in the amount of soil. The increase was higher in the red soil than black soil, but the number of polyhedra adsorbed per unit weight of soil decreased as the amount of soil increased; the rate of decrease being faster in black soil than in red soil. The adsorption of polyhedra of nuclear polyhedrosis virus of Heliothis armigera is discussed based on certain physico-chemical properties of both red and black soils.

The infectivity of polyhedra of nuclear polyhedrosis virus (N.P.V.) after passage through gut of an insectpredator.

Polyhedral inclusion bodies (P.I.B) of a nuclear polyhedrosis virus of Heliothis punctigera (Lep.) were found in excreta of Nabis tasmanicus (Het.), a predator of H. punctigera. An immunofluorescent counting method was used to estimate numbers of P.I.B. Bioassays demonstrated that no loss of infectivity occurred during passage through the gut.

Activation of latent virus infections in larvae of Adoxophyes orana (Lepidoptera: Tortricidae) and Barathra brassicae (Lepidoptera: Noctuidae) by foreign polyhedra

The number of larvae containing polyhedra increased when larvae of Adoxophyes orana and Barathra brassicae were fed on polyhedra of nuclear polyhedrosis virus (NPV) of the reciprocal species. Comparison of restriction endonuclease EcoRI cleavage patterns of DNA isolated from polyhedra used as inocula and from polyhedra obtained after cross-inoculation showed that cross infection did not occur. The observations indicate that latent viruses were activated in both insects. Activation of the A. orana latent NPV with polyhedra of a cytoplasmic polyhedrosis virus (CPV) of B. brassicae, and cross-inoculation with an extract prepared from healthy larvae indicated that an activating agent does not have to be a component of nuclear polyhedra.