Polydimethylsiloxane Nanoparticles Research Articles

PDMS nanoparticles-decorated PDMS substrate promotes adhesion, proliferation and differentiation of skin cells

Polydimethylsiloxane (PDMS) is known to be a common substrate for various cell culture-based applications. However, native PDMS is not very conducive for cell culture and hence, surface modification via cell adhesion moieties is generally needed to make it suitable especially for long-term cell culture. To address this issue, we propose to coat PDMS nanoparticles (NPs) on the surface of PDMS film to improve adhesion, proliferation and differentiation of skin cells. The proposed modification strategy introduces necessary nanotopography without altering the surface chemical properties of PDMS. Due to resemblance in the mechanical properties of PDMS with skin, PDMS NPs can recreate the native extracellular nanoenvironment of skin on the PDMS surface and provide anchoring sites for skin cells to adhere and grow. Human keratinocytes, representing 95% of the epidermal skin cells maintained their characteristic well-spread morphology with the formation of interconnected cell-sheets on this coated PDMS surface. Moreover, our in vitro immunofluorescence studies confirmed expression of distinctive epidermal protein markers on the coated surface indicating close resemblance with the native skin epidermis. Conclusively, our findings suggest that introducing nanotopography via PDMS NPs can be an effective strategy for emulating the native cellular functions of keratinocytes on PDMS based cell culture devices.

A highly robust, non‐fluorinated, and economical PDMS‐based superhydrophobic flexible surface with repairable and flame‐retardant properties

AbstractNature‐inspired artificial superhydrophobic surfaces have attracted tremendous attention due to their potential applications in various fields. However, the use of toxic chemicals and expensive nanoparticles with limited durability has restricted the practical applications of these surfaces. Here, we proposed a novel and non‐fluorinated technique to fabricate robust and flexible polydimethylsiloxane (PDMS)‐based superhydrophobic surface. Fabrication involved two simple steps: preparation of superhydrophobic nanoparticles by burning PDMS, and fabrication of flexible superhydrophobic PDMS surface (SPS) by dispersing particles in a solvent containing PDMS solution and curing agent. Effect of PDMS particle content on morphology and wettability was examined and 10 wt.% PDMS nanoparticles demonstrated superior superhydrophobicity with a water contact angle of 156° and sliding angle of ~6°. The SPS displayed excellent mechanical robustness in a sandpaper abrasion test and showed no apparent wettability deterioration even when stretched by up to 50%. Additionally, surface maintained outstanding superhydrophobicity even after 300 cycles of mechanical deformation. Impressively, damaged surface can restore its superhydrophobicity after removing the damaged layer using sandpaper abrasion and displayed exceptional chemical, thermal, flame‐retardant, and long‐lasting superhydrophobic properties. The work provides an innovative, low‐cost, and environment‐friendly strategy for designing robust and flexible superhydrophobic surfaces that can be used in real‐life applications.

A simple, robust and scalable route to prepare sub-50 nm soft PDMS nanoparticles for intracellular delivery of anticancer drugs

Soft nanoparticles (NPs) have recently emerged as a promising material for intracellular drug delivery. In this regard, NPs derived from polydimethylsiloxane (PDMS), an FDA approved polymer can be a suitable alternative to conventional soft NPs due to their intrinsic organelle targeting ability. However, the available synthesis methods of PDMS NPs are complicated or require inorganic fillers, forming composite NPs and compromising their native softness. Herein, for the first time, we present a simple, robust and scalable strategy for preparation of virgin sub-50 nm PDMS NPs at room temperature. The NPs are soft in nature, hydrophobic and about 30 nm in size. They are stable in physiological medium for two months and biocompatible. The NPs have been successful in delivering anticancer drug doxorubicin to mitochondria and nucleus of cervical and breast cancer cells with more than four-fold decrease in IC50 value of doxorubicin as compared to its free form. Furthermore, evaluation of cytotoxicity in reactive oxygen species detection, DNA fragmentation, apoptosis-associated gene expression and tumor spheroid growth inhibition demonstrate the PDMS NPs to be an excellent candidate for delivery of anticancer drugs in mitochondria and nucleus of cancer cells.

Stable sub-100 nm PDMS nanoparticles as an intracellular drug delivery vehicle

For the past few decades, polydimethylsiloxane (PDMS) elastomer has been used in plethora of biomedical applications. However, PDMS has not much been explored for intracellular drug delivery since the preparation of sub-100 nm particles, preferred for such kind of applications is extremely difficult owing to its innate nature to form a film. In this work, we have performed molecular dynamics (MD) simulation for developing a strategy to restrict the inherent film-forming tendency of PDMS for obtaining stable sub-100 nm PDMS nanoparticles. MD simulation results suggest that introduction of hydroxyl groups on the surface of PDMS improves its stability in the form of nanoparticles. Based on the MD simulation results, for the first time, sub-100 nm PDMS nanoparticles are prepared via in situ surface modification of PDMS with sodium hydroxide inside nanoemulsion droplets. The synthesized nanoparticles are 30–40 nm in size, extremely soft in nature, moderately hydrophobic and stable in phosphate buffered saline. In vitro results demonstrate the synthesized PDMS nanoparticles to possess excellent biocompatibility and an intrinsic capability of selective localization in mitochondria of cancer cells. Furthermore, efficient mitochondrial delivery of anticancer drug doxorubicin through PDMS nanoparticles advocates for their suitability as a potential candidate for developing advanced nanomedicine.

Correction to "Direct Intranuclear Anticancer Drug Delivery via Polydimethylsiloxane Nanoparticles: In Vitro and In Vivo Xenograft Studies".

RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to “Direct Intranuclear Anticancer Drug Delivery via Polydimethylsiloxane Nanoparticles: In Vitro and In Vivo Xenograft Studies”Gargi MishraGargi MishraMore by Gargi Mishra, Souryadeep BhattacharyyaSouryadeep BhattacharyyaMore by Souryadeep Bhattacharyya, Vipul BhatiaVipul BhatiaMore by Vipul Bhatia, Bushra AteeqBushra AteeqMore by Bushra Ateeq, Ashutosh SharmaAshutosh SharmaMore by Ashutosh Sharmahttp://orcid.org/0000-0002-2653-0806, and Sri Sivakumar*Sri SivakumarMore by Sri Sivakumarhttp://orcid.org/0000-0002-6472-2702Cite this: ACS Appl. Mater. Interfaces 2019, 11, 42, 39395Publication Date (Web):October 14, 2019Publication History Published online14 October 2019Published inissue 23 October 2019https://doi.org/10.1021/acsami.9b17603Copyright © 2019 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceArticle Views486Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts

New nanostructure of polydimethylsiloxane coating as a solid-phase microextraction fiber: Application to analysis of BTEX in aquatic environmental samples.

Electrospinning technique was used to convert polydimethyl siloxane (PDMS) sol-gel solution to a new nanostructure on a stainless steel wire. The surface morphology of the fiber was observed by scanning electron microscopy (SEM). It showed a diameter range of 30-60nm for PDMS nanoparticles with a homogeneous and porous surface structure. The applicability of this coating was assessed for the headspace SPME (HS-SPME) of benzene, toluene, ethylbenzene and xylenes (BTEX) from water samples followed by gas chromatography-mass spectrometry. The important parameters affecting extraction efficiency such as extraction time and temperature, desorption conditions, agitation rate and ionic strength were investigated and optimized. Under the optimized conditions, LODs and LOQs of 0.3-5μgL(-1) and 1-10μgL(-1) were obtained, respectively. The method showed linearity in the broad range of 1-5000μgL(-1) with correlation coefficient of >0.99. Inter-day and intra-day precisions of the developed method ranged from 2.43% to 6.54% and from 5.24% to 13.73%, respectively. The thermal stability of the fiber was investigated on stainless steel wire. It was found to be durable at 260°C for more than 360min. Furthermore, the proposed method was successfully applied for quantification of BTEX in real water samples.