Polycyclic Aromatic Hydrocarbons In Seawater Research Articles

Simulation and parameter determination of the net sorption of phenanthrene by sediment particles

The distribution of polycyclic aromatic hydrocarbons (PAHs) in the ocean is affected by the sorption-desorption process of sediment particles. This process is determined by the concentration of PAHs in seawater, water temperature, and organic matter content of sediment particles. Quantitative relationships between the net sorption rates (=the difference of sorption and desorption rates) and these factors have not been established yet and used in PAH transport models. In this study, phenanthrene was chosen as the representative of PAHs. Three groups of experimental data were collected to address the dependence of the net sorption processes on the initial concentration, water temperature, and organic carbon content representing organic matter content. One-site and two-compartment mass-transfer models were tested to represent the experimental data using various parameters. The results showed that the two-compartment mass-transfer model performed better than the one-site mass-transfer model. The parameters of the two-compartment mass-transfer model include the sorption rate coefficients kafand kas (L g−1 min−1), and the desorption rate coefficients kdf and kds (min−1). The parameters at different temperatures and organic carbon contents were obtained by numerical simulations. Linear relationships were obtained between the parameters and water temperature, as well as organic carbon content. kaf, kas and kdf decreased linearly, while kds increased linearly with temperature. kaf, kas and kdf increased linearly, while kds decreased linearly with organic carbon content. The r2 values between the simulation results based on the relationships and the experimental results reached 0.96–0.99, which supports the application of the model to simulate sorption-desorption processes at different water temperatures and organic carbon contents in a realistic ocean.

Polycyclic aromatic hydrocarbons in seawater after the mysterious oil spill of 2019 on the Pernambuco coast, northeast Brazil

In 2019, one of Brazil's most significant environmental disasters occurred, involving an oil spill that directly affected Pernambuco state. Contamination along the coast was evaluated by the quantification of polycyclic aromatic hydrocarbons (PAHs) in fifty seawater samples collected in the summer and winter of 2021. Analysis using fluorescence spectroscopy revealed that for all the samples, levels of dissolved/dispersed petroleum hydrocarbons (DDPHs) were higher than the regional baseline for tropical western shores of the Atlantic Ocean. GC–MS analyses quantified 17 PAHs in the samples, with highest total PAHs concentrations of 234 ng L−1 in summer and 33.3 ng L−1 in winter, which were consistent with the highest risks observed in ecotoxicity assays. The use of diagnostic ratios showed that the coast was impacted by a mixture of PAHs from petrogenic and pyrolytic sources. The results indicated the need for continuous monitoring of the regions affected by the 2019 spill.

Air‐Sea Gas Exchange and Its Potential Influence on the Regional Fate of Polycyclic Aromatic Hydrocarbons in the East China Marginal Sea

AbstractTo investigate the air‐sea gas exchange and its potential influence on the regional fate of polycyclic aromatic hydrocarbons (PAHs) in the East China Marginal Seas (ECMS), which consist of the East China Sea (ECS) and Yellow Sea (YS), we collected air and surface seawater samples of this area in the summer 2018 and winter 2019, respectively. Generally, PAHs underwent a strong volatilization process in the ECMS in both summer and winter. Good correlations between wind speed and the magnitude of air‐sea gas exchange were found for low molecular weight PAHs, suggesting the rate of their air‐sea gas exchange was influenced by the static stability of overlying atmosphere. However, such an influence for high molecular weight PAHs was constrained by their low Henry's law constant. Dissolved concentration of PAHs in surface seawater was another key factor regulating their air‐sea gas exchange, which not only influenced the rate of air‐sea gas exchange but also was involved in the exchange direction. Higher air‐sea gas exchange fluxes of PAHs in winter were attributed to their increasing dissolved concentrations in seawater during this season. A mass conservation analysis revealed a huge volatilization loss of PAHs from seawater to atmosphere, suggesting air‐sea gas exchange might be a key process to modulate the distribution and occurrence of PAHs in the ECS and YS. Such a loss of PAHs in seawater might be compensated by the sediment resuspension, which implied that the sedimentary deposit could serve as a secondary source of PAHs in seawater and overlying atmosphere.

Environmental profile, potential sources, and ecological risk of polycyclic aromatic hydrocarbons in a typical coastal bay and outer bay area.

As a class of persistent organic pollutants, polycyclic aromatic hydrocarbons (PAHs) are widely present and accumulate in multimedia environments. The pollution characteristics, spatiotemporal distribution, potential sources, influencing factors, and ecological risks of 16 PAHs were investigated in the water-sediment system of the Hangzhou Bay and outer bay area (HZB and OBA, respectively). The total concentrations of 16 PAHs (∑PAHs) were 220 ± 97.0 and 130 ± 36.0ng/L in the seawater and 343 ± 179 and 505 ± 415μg/kg (dry weight) in the sediments of the HZB and OBA, respectively. The pollution level of PAHs in the HZB seawater was higher than that in the OBA seawater, but the opposite result was found in the sediments. Moreover, ∑PAHs exhibited high temporal variability in the HZB seawater (rainy season > dry season), whereas ∑PAHs in the sediments showed no significant difference between seasons. The molecular diagnostic ratio method was used to identify pollution sources and showed that the PAHs in seawater came from different pollution sources (fuel combustion and petroleum), whereas the PAHs in the sediments originated from coincident sources (mixed combustion). Correlation analysis revealed that temperature was positively related to ∑PAHs, whereas salinity was negatively related to seawater ∑PAHs values. Ecological risk assessment demonstrated that the potential for adverse ecological effects was low to moderate in seawater but moderate to high in the sediments.

Spatiotemporal PAH levels in surface water of the Southeastern Black Sea; baseline study from the Giresun shores

Samples of surface water were collected seasonally along the 121 km long Giresun coastline to comparatively evaluate the source, distribution, and ecological risk of 16 polycyclic aromatic hydrocarbons (PAHs). The total PAHs levels, varied between 47.42 and 576.69 ng L−1 with the mean of 161.5 ng L−1, were consistent with seawater studies worldwide. Diagnosis rates evinced that PAHs in seawater along the Giresun coastline are primarily sourced from biomass, coal and petroleum combustion and petroleum at lesser extent. The three-ring PAHs (40.2 %) was proportionally high, followed by 2-ring PAHs (26.5 %). The toxicity equivalent quotient (TEQ) and risk quotients (RQ) were employed to assess ecological risk posed by PAHs in Giresun coastal seawater. As a result, the ecological risk in all seasons was at moderate to high risk level, except for autumn, which shows low to moderate risk according to TEQ. RQ values of ∑PAHs displayed low to moderate ecological risk.

Occurrence and ecological risks of PAHs in the dissolved and particulate phases of coastal surface water of Taiwan

Surface seawater were collected from seven sites along the coast of Taiwan. A total of 100 L seawater was pre-concentrated from each site and the concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) in the dissolved and particulate phases were analyzed. The characteristics, potential sources, and ecological risks of PAHs in seawater were assessed. The concentration of total PAHs (TPAHs) in the dissolved phases ranged between 3.3–25.8 ng/L, with an average of 14.7 ± 8.9 ng/L, classified as slightly contaminated, while the particulate phase ranged between 3.3–22.4 ng/L (8.5 ± 6.8 ng/L). The TPAHs concentrations in seawater are mainly dominated in the dissolved phase (60.4 ± 22.0%). The partition coefficients of PAHs in the particulate and dissolved phases were linearly correlated significantly with octanol–water partition coefficient (r = 0.744; p<0.01), indicating that hydrophobicity plays a key role in the partitioning behavior of PAHs. The dissolved phase PAHs in seawater were dominated by 3-ring PAHs (84.8 ± 3.9%), while the particulate phase was dominated by 5-ring PAHs (88.4 ± 9.1%). According to the diagnostic ratio, coal and biomass combustion are the main sources of PAHs, while petroleum and petroleum combustion sources also contributed to the northeast coast. The results of toxic equivalence quotients and mean risk quotient showed that the toxicity and risks of PAHs in the particulate phases were higher than those in the dissolved phases. Furthermore, the proportion of 5-ring PAHs in the dissolved phase was low (5.9 ± 4.3%), but relatively contributed high potential toxicity.

Contamination Assessment of Mangrove Ecosystems in the Red Sea Coast by Polycyclic Aromatic Hydrocarbons.

Mangroves are known as a naturally based solution for climate mitigation and adaptation. Mangroves are at a potential risk of degradation by contaminants such as polycyclic aromatic hydrocarbons (PAHs). In this study, sixteen priority PAHs were analyzed and characterized in forty samples of mangrove seawater and mangrove sediments collected from two coastal areas (i.e., Sharm and Khor Rabigh) along the Red Sea Coast of Rabigh city in August 2013. We found that the average concentration of total PAH in mangrove sediments in the Sharam area (22.09 ng/kg) was higher than that in the Alkhor area (6.51 ng/kg). However, the average concentration of the total PAH in the mangrove seawater in the Alkhor area (9.19 ng/L) was double that in the Sharam area (4.33 ng/L). Phenanthrene and pyrene were the major components in both the mangrove seawater and sediment in all the investigated areas. We observed that the abundance of PAHs with 2–3 aromatic rings was dominant in sediment samples collected from both study areas. This abundance was also observed in seawater from the Sharam area. However, seawater samples from the Alkhor area had abundant PAHs with four aromatic rings. The majority of PAHs in sediment samples of both study areas originated from petrogenic sources, whereas the majority of PAHs in seawater samples originated from pyrogenic sources.

Method Development for Low-Concentration PAHs Analysis in Seawater to Evaluate the Impact of Ship Scrubber Washwater Effluents

A naval ship’s exhaust gas scrubber may discharge polycyclic aromatic hydrocarbons (PAHs) into seawater. Due to the high lipophilicity and low water solubility of PAHs, their concentrations in seawater are extremely low, making them difficult to detect or accurately determine. To accurately assess the impact of scrubber washwater effluent on the PAHs concentration of seawater, appropriate analysis methods must be established. In this study, a large-volume pre-concentration water sampler was used onboard to concentrate PAHs in surface seawater (100 L) from four sites offshore of southern Taiwan. The quantitative and qualitative analysis of dissolved PAHs in seawater and quality control samples were implemented using a GC/MS system with the aid of internal and surrogate standards. Results showed that the field and equipment blank samples of quality control samples were lower than twice the detection limit. The detection limit of individual PAHs is between 0.001 (naphthalene, NA) and 0.014 ng/L (dibenzo[a,h]anthracene, DBA), which meets the requirements for evaluating PAHs in seawater (that is, less than the maximum permissible concentrations (MPCs)). The concentration of total PAHs (TPAHs) in the four seawater samples ranged from 2.297 to 4.001 ng/L and had an average concentration of 3.056 ± 0.727 ng/L. The concentrations of 16 PAHs were determined in each seawater sample, indicating that the analytical method in this study is suitable for the determination of low-concentration PAHs in seawater. Phenanthrene (PHE) is the most dominant compound in seawater samples accounting for 59.6 ± 12.6% of TPAHs, followed by fluorine (FL) accounting for 8.5 ± 3.7%. The contribution of high-ring PAHs to TPAHs is not high (0.5–9.2%), but the observed concentrations can cause a higher risk to aquatic organisms than low-ring PAHs. The diagnostic ratio showed that the sources of PAHs in the seawater collected offshore of southern Taiwan may include mixed sources such as petrogenic, petroleum combustion, and biomass combustion. The results can be used for regular monitoring, which contributes to pollution prevention and management of the marine environment.

Polycyclic aromatic hydrocarbons in seawater, surface sediment, and marine organisms of Haizhou Bay in Yellow Sea, China: Distribution, source apportionment, and health risk assessment

The pollution status of polycyclic aromatic hydrocarbons (PAHs) in seawater, surface sediment, and marine organisms was investigated in Haizhou Bay, which is a traditional marine fish farming region in China. The total concentrations of PAHs in seawater, surface sediment, and marine organisms were 12.4–40.3 ng/L (average 24.8 ng/L), 183.2–496.6 ng/g (average 293.5 ng/g), and 228.1–679.9 ng/g (average 392.6 ng/g), respectively. Source analysis results showed that the PAH sources for seawater and marine organisms were coal and biomass combustion (66.53%), petroleum (28.94%), and traffic (4.52%), while those for the surface sediment were traffic (48.14%), coal and biomass combustion (40.56%). The lifetime cancer risk increment (ILCR) values of marine organisms in the Haizhou Bay were less than 10−6, indicating no carcinogenic risk. In the future, this study can be used as a reference to understand the distribution and interrelation of PAHs in other semi-enclosed bays in the world.

Dissolved polycyclic aromatic hydrocarbons from the Northwestern Pacific to the Southern Ocean: Surface seawater distribution, source apportionment, and air-seawater exchange

Polycyclic aromatic hydrocarbons (PAHs) as a group of toxic and carcinogenic compounds are large scale globally emitted anthropogenic pollutants mainly emitted into the atmosphere. However, atmospheric transport cannot fully explain the spatial variability of PAHs in the marine atmosphere and seawater. It is hypothesized that PAHs accumulated in seawater and ocean circulation can also influence PAHs observed in air above the ocean. In order to investigate PAHs in seawater as a potential secondary source to air, we collected paired air and seawater samples during a research cruise from China to the Antarctic in 2018–2019, covering the Pacific Ocean, the Indian Ocean, and the Southern Ocean. Summed concentrations of 28 analyzed PAHs in seawater were highest in the Pacific Ocean (4000 ± 1400 pg/L), followed by the Indian Ocean (2700 ± 1000 pg/L), and the Southern Ocean (2300 ± 520 pg/L). Three-ringed PAHs dominated the composition profile. We found that PAH levels in the Pacific and Indian Oceans were strong inversely correlated with salinity and distance to the coastline. This suggests that riverine inputs and continental discharges are important sources of PAHs to the marine environment. Derived air-seawater fugacity ratios suggest that net fluxes of PAHs were from seawater to the air in the Pacific and Indian Oceans at 9.0–8100 (median: 1600) ng/m2/d and 290–2000 (median: 1300) ng/m2/d, respectively. In the Southern Ocean, the net flow of PAHs was from air to seawater with a flux of -1000–450 (median: -82) ng/m2/d. Source apportionment from two different models suggested that the largest contribution to total PAHs was from petrogenic sources (44–57%), followed by coal/wood combustion (30–31%), fossil fuel combustion (15%), and engine combustion emissions (2.8–9.5%). Higher contributions from petrogenic sources were found at sites close to coastal regions. Both coal/wood combustion and petrogenic sources are responsible for the PAH concentrations detected in the Indian and Southern Oceans.

Spatiotemporal occurrence, sources and risk assessment of polycyclic aromatic hydrocarbons in a typical mariculture ecosystem

The spatiotemporal variations, influencing factors and potential sources, as well as the ecological/health risks of polycyclic aromatic hydrocarbons (PAHs) were systematically investigated in seawater, sediment, and fish from Xiangshan Bay, China, one of the most important and oldest domestic marine aquaculture bases. The average concentrations of ΣPAHs in seawater, sediment and fish were 150 ± 70.0 ng/L, 276 ± 271 μg/kg (dry weight, dw), and 434 ± 151 μg/kg (dw), respectively. Naphthalene, phenanthrene, fluoranthene, benzo(b)fluoranthene and pyrene were the dominant contaminants in all samples. The highest PAH concentrations in the seawater and sediment samples occurred in the inner bay where the mariculture and industry are clustered. Seasonal differences were observed in the seawater samples but not in the sediment samples. Among all 15 fish species, large yellow croaker (Larimichthys crocea) (775 μg/kg (dw)), red drum (Sciaenops ocellatus) (749 μg/kg (dw)), and flathead grey mullet (Mugil cephalus) (637 μg/kg (dw)) had relatively high PAH accumulation concentrations in muscle tissue. According to the molecular diagnostic ratio method, the PAHs in seawater mainly originated from a mixed source of petroleum and combustion, whereas biomass/coal combustion sources were identified for sediment. The results obtained from the risk quotient (for seawater), sediment quality guidelines and toxic equivalence quotients (for seawater and sediment) methods showed that the ecological risks posed by PAHs were generally at a low to moderate level. Potentially toxic effects existed from PAH-contaminated fish consumption, and the resulting potential carcinogenic risk was also slightly higher than the recommended guidelines (10−6).

An integrated approach using chemical ecological risk assessment and multi-integrated biomarker indexes approach to assess pollution: A case study of Ruditapes philippinarum in four bays on the Shandong Peninsula in China

Considering the ecological risks of polycyclic aromatic hydrocarbons (PAHs) to the marine environment, it is urgent to find scientific and effective monitoring methods. In this study, an integrated approach combining chemical ecological risk assessment and multi-integrated biomarker indexes approach was used to assess the marine environment. Samples included seawater, sediments, and clam Ruditapes philippinarum were collected from four bays on the Shandong Peninsula, China in the four seasons of 2019. The concentrations, composition, potential sources, and ecological risk of PAHs were investigated in seawater and sediments. Risk quotient (RQ) and sediment quality guidelines (SQGs) were calculated to assess the ecological risks of PAHs in seawater and sediment, respectively. And then, clam Ruditapes philippinarum's multi-level biological response, including its ethoxyresorufin-O-deethylase (EROD), glutathione S-transferase (GST), superoxide dismutase (SOD), lipid peroxidation (LPO), and acetylcholinesterase (AChE) were investigated in-depth, by which multi-integrated biomarker indexes approach were calculated to evaluate marine environmental quality. Taken together, the results showed that the concentration of PAHs was in good agreement with the response of biomarkers, and the usefulness of the combined use of chemical ecological risk assessment and integrated biomarker indexes to assess PAHs pollution was verified.

Net volatilization of PAHs from the North Pacific to the Arctic Ocean observed by passive sampling.

The North Pacific-Arctic Oceans are important compartments for semi-volatile organic compounds' (SVOCs) global marine inventory, but whether they act as a "source" or "sink" remains controversial. To study the air-sea exchange and fate of SVOCs during their poleward long-range transport, low-altitude atmosphere and surface seawater were measured for polycyclic aromatic hydrocarbons (PAHs) by passive sampling from July to September in 2014. Gaseous PAH concentrations (0.67-13ngm-3) were dominated by phenanthrene (Phe) and fluorene (Flu), which displayed an inverse correlation with latitude, as well as a significant linear relationship with partial pressure and inverse temperature. Concentrations of PAHs in seawater (1.8-16ngL-1) showed regional characteristics, with higher levels near the East Asia and lower values in the Bering Strait. The potential impact from the East Asian monsoon was suggested for gaseous PAHs, which - similar to PAHs in surface seawater - were derived from combustion sources. In addition, the data implied net volatilization of PAHs from seawater into the air along the entire cruise; fluxes displayed a similar pattern to regional and monthly distribution of PAHs in seawater. Our results further emphasized that air-sea exchange is an important process for PAHs in the open marine environments.

Spatial-temporal distribution of polycyclic aromatic hydrocarbons in seawater at West Nanao Bay, Noto Peninsula

Spatial-temporal distribution of polycyclic aromatic hydrocarbons in seawater at West Nanao Bay, Noto Peninsula

A turn-off luminescent europium probe for efficient and sensitive detection of some low molecular weight polycyclic aromatic hydrocarbons in seawater

Eu(III)-(CCA)2 complex probe was applied for the direct detection of polycyclic aromatic hydrocarbons PAHs naphthalene, anthracene, acenaphthene or fluorene in seawater samples were collected from Suez Bay. The low limit of detection and the quantification limit were calculated for all compounds under study. The selectivity of the probe was characterized through studying the effect of some interfering ions. The results obtained from fluorimetric method using Eu(III)-(CCA)2 probe was validated through using gas chromatography and arecoverymethod. The developed Eu(III)-(CCA)2 probe has the ability for detecting PAHs in rapid and straightforward procedure even in presence of interfering metal ions. The data indicated that the Eu(III)-(CCA)2 probe is suitable for direct and efficient determination of PAHs compounds from seawater.

Distribution and sources of polycyclic aromatic hydrocarbons in the water column of Kongsfjorden, Arctic

Kongsfjorden is known for its characteristic multi-layer water mass formed by the convergence of freshwaters from nearby glaciers and rivers and saline water from the Atlantic and Arctic. The distribution of polycyclic aromatic hydrocarbons (PAHs) in the water column of Kongsfjorden was investigated and their potential sources were analyzed. The total concentrations of 16 PAHs in the surface seawater and river water were in the range of 33.4−79.8 ng/L (mean 48.5 ng/L) and 2.3−201.4 ng/L (mean 126.1 ng/L), respectively. Horizontally, PAHs were mainly concentrated around river estuaries and the glacier front in the dissolved phase. Vertically, the PAHs in the particulate phase followed surface-enrichment and depth-depletion patterns in most stations, with the maximum concentration found at 50 m depth in the central area of Kongsfjorden. The compositions of PAHs in seawater and rivers were similar, with two-ring and tricyclic PAHs comprising the majority of the dissolved and particulate phases. PAHs found in Kongsfjorden waters appeared to be derived from multiple sources such as petroleum and coal combustion. PAHs in the bay mouth of Kongsfjorden were mainly introduced by the West Spitsbergen Current and the Arctic waters, while in the inner bay, atmospheric deposition and local sources were the major contributors. The distribution of PAHs was mainly attributed to the suspended particulate distribution.

Development of a computational method to quantify the partitioning of polycyclic aromatic hydrocarbons in seawater into dissolved and droplet forms

The ecological risk and potential injury from polycyclic aromatic hydrocarbons (PAHs) from spilled crude oil in water is dependent on whether they are dissolved or associated with droplets/particles. Using oil collected from the Deepwater Horizon incident, laboratory experiments were conducted to develop a computational method to determine the physical state of PAHs in sampled seawater and compare these to results from methods designed to physically separate dissolved and droplet oil used during the spill. The analytical results were used to develop a method to calculate droplet/particulate concentrations from unfractionated water samples based on oil composition and allocation of minimally soluble oil components to the droplet fraction. The computational method is less labor-intensive and costly than field fractionation and can be used to optimize the use of previously collected data. The results also showed that physical separation can result in insoluble high-molecular-weight PAHs being present in the filtrate (i.e., the “dissolved” fraction), potentially leading to an overestimate of dissolved components.

Temporal Variations of Polycyclic Aromatic Hydrocarbons in the Seawater at Tsukumo Bay, Noto Peninsula, Japan, during 2014-2018.

Concentrations of phase-partitioning 13 polycyclic aromatic hydrocarbons (PAHs) in seawater were investigated in the Tsukumo Bay, Noto Peninsula, Japan, during 2014–2018, to improve the understanding of the environmental behavior of PAHs in the coastal areas of the Japan Sea. Total PAH (particulate plus dissolved) concentrations in surface seawater were in the range 0.24–2.20 ng L−1 (mean 0.89 ng L−1), an order of magnitude lower than the mean values observed in the Japan Sea in 2008 and 2010. Although the PAH contamination levels during 2014–2018 were significantly lower than those in the East China Sea, the levels increased from 2014 to 2017 and were maintained at the higher level during 2017–2018. The main sources of particulate and dissolved PAHs during 2014–2018 were combustion products, of which the former were more influenced by liquid fossil-fuel combustion and the latter by biomass or coal combustion. The increase in particulate PAH concentrations in October–December during 2014–2018 was due to the impact of PAH-rich airmasses transported from the East Asian landmass in the northwesterly winter monsoon winds. The increase in dissolved PAH levels during July–September in 2014, 2016, 2017, and 2018 indicates that the Tsukumo Bay is possibly impacted by the PAH-rich summer continental shelf water transported by the Coastal Branch of the Tsushima Warm Current, which flows into the Japan Sea from the East China Sea.

Identification polycyclic aromatic hydrocarbons (PAHs) in the Banyuasin river estuary, South Sumatera

The Banyuasin river estuary is an aquatic area located on the east coast of south Sumatera and receives waste input from activities along with the river flow. One of organic pollutants that toxicate marine life is polycyclic aromatic hydrocarbons (PAHs). This study was aimed to identify and determine the concentration of PAHs in seawater and sediment samples from Banyuasin river estuary. A sampling of seawater and sediment was carried out in July 2018. Seawater samples were taken using water sampler and sediment samples were taken using grab at 5 research stations. The concentration of PAHs was analyzed by Gas Chromatography-Flame Ionization Detector. The results showed that the PAH content in the seawater ranged from 92.34 to 320.09 ppb while the content of PAHs in the sediment ranges from 3.16-23.28 ppb. Overall, the PAHs content in the seawater Banyuasin river estuary had exceeded the threshold limit value stated by the Indonesian Ministry of Environment, which was 3 ppb or 0.003 ppm

Biodegradation of aliphatic and polycyclic aromatic hydrocarbons in seawater by autochthonous microorganisms

The bioremediation of petroleum spills in marine environments is still a challenge nowadays, and the technology available towards this goal must be improved. The search for efficient and applicable systems requires the prospection, isolation and characterization of autochthonous microorganisms with potential for bioremediation. Here, microorganisms were isolated from “Lagoa do Peixe” National Park, a site with a history of frequent petroleum spills in Brazil. The isolation methodology was based on the enrichment of microorganisms able to biodegrade petroleum hydrocarbons. After preliminary tests of 2,3,5-triphenyl tetrazolium chloride (TTC) redox indicator, two bacteria were able to produce biosurfactants and metabolize complex carbon sources in a solid medium: Bacillus methylotrophicus SSNPLPB5 and Pseudomonas sihuiensis SNPLPB7. Biodegradation assays using aliphatic and polycyclic aromatic hydrocarbons (PAHs) sources showed that both bacteria are able to biodegrade: (i) up to 92.1% and 42.4% of the medium (C8 to C19) and long (C20 to C33) chain aliphatic contaminant fraction, respectively; (ii) 31.1% of isoprenoid (pristane) and (iii) 46% of anthracene, 33.9% of phenanthrene and 35.3% of pyrene, after 46 days of incubation in seawater. The isolates were shown to produce biosurfactants such as surfactins, iturins, mono- and di-rhamnolipids, which reduced the surface tension, pH and showed emulsifying activity of the cell-free supernatant at the end of the incubation time, with potential application in petroleum spills bioremediation.