Polycyclic Aromatic Hydrocarbons In Milk Research Articles

The investigation of polycyclic aromatic hydrocarbons (PAHs) in milk and its products: A global systematic review, meta-analysis and health risk assessment

Exposure to polycyclic aromatic hydrocarbons (PAHs) in food is inevitable. Present study was conducted to investigate PAH contamination in milk and dairy products. International databases were investigated to collect data regarding the PAHs in milk and dairy products. Furthermore, health risks were calculated based on the benzo[a]pyrenee (BaP) equivalent dose. Highest concentrations of PAHs were phenanthrene for milk, babe products and cheese (0.47, 2.14 and 9.59 μg/kg, respectively) and acenaphthene (9.59 μg/kg) for cheese. Also, lowest concentration of PAHs found in milk, baby products, was 0.03 and 0.03, (μg/kg), belonging to dibenz[a,h]anthracene, naphthalene, and in yogurt and cheese was 0.004 and 0.08 (μg/kg), related to benzo [b] flouranthene respectively. According to WHO region, acenaphthylene, phenanthrene and naphthalene had highest concentration, while benzo [a] pyrene, dibenz[a,h]anthracene, acenaphthene and dibenz [a,h]anthracene had lowest concentration. Risk assessment of PAHs revealed be different in countries. Therefore, regulatory supports is necessary to content contaminants.

Rapid Determination of Oxygenated and Parent Polycyclic Aromatic Hydrocarbons in Milk Using Supercritical Fluid Chromatography-Mass Spectrometry.

Liquid milks are consumed worldwide in large amounts, especially by adolescents and infants. Thus, their health quality linked with polycyclic aromatic hydrocarbon (PAH) contamination has attracted great concern. This study developed a rapid and sensitive supercritical fluid chromatography (SFC)-MS method to determine two typical oxygenated PAHs (OPAHs) and EU 15+1PAHs except for benzo[k]fluoranthene (BkF) in three types of liquid milks: 10 ultra heat treated (UHT) milks, 8 pasteurized milks, and 4 extended-shelf-life pasteurized milks. The instrumental analysis was 15 min with a recovery of 67.66-118.46%, a precision of 1.45-14.68%, detection limits of 0.04-0.24 μg/kg, and quantification limits of 0.13-0.78 μg/kg. We found 9-fluorenone, anthraquinone, 15 EU priority PAHs, and benzo[a]pyrene toxic equivalent quantity (BaPeq) in the 22 milk samples, which were 0.32-1.56 μg/kg, 0.40-1.74 μg/kg, 0.57-8.48 μg/kg, and 0.01-17.42 μg/kg, respectively. The UHT milks and whole fat milks showed higher PAH concentrations than other investigated samples, where the maximum levels of BaP and PAH4 were 0.77 and 3.61 μg/kg, respectively. PAH4 dominantly contributed to the PAH8 concentration and was detected in 73% and 32% of samples at more than 1.0 and 2.0 μg/kg, respectively. The results suggest that raw milks should be strictly monitored and extensively investigated for PAH4 and BaP concentrations for future risk assessment, limitations, and dietary guidance.

Presence of Halogenated Polycyclic Aromatic Hydrocarbons in Milk Powder and the Consequence to Human Health.

Recent reports of the presence of halogenated derivatives of polycyclic aromatic hydrocarbons (PAHs) in human foods of animal origin, such as chlorinated (ClPAHs) and brominated (BrPAHs) PAHs, suggest that their contamination in dairy products may also pose a human health risk. This study used GC/Orbitrap-MS to analyze 75 congeners of halogenated PAHs and parent PAHs in milk and creaming powder samples commonly found in grocery stores in Sri Lanka and Japan. Our investigation revealed a total of 31 halogenated PAHs (HPAHs) in the samples. The concentrations of total parent PAHs in the samples from Sri Lanka and Japan ranged from not detected (n.d.)–0.13 and <0.001–16 ng/g dry weight (d.w.). Total ClPAHs and BrPAHs in the samples ranged from 0.01–3.35 and 1.20–5.15 ng/g (d.w.) for Sri Lanka, and 0.04–2.54 and n.d.–2.03 ng/g d.w. for Japan, respectively. The ClPAHs were dominated by chlorinated-pyrene, -fluoranthene, and -benzo[a]pyrene congeners, whereas the BrPAHs were dominated by brominated-naphthalene and -pyrene congeners. The toxic assessment estimated based on the intake of toxic equivalency quotients (TEQs) for target compounds in milk powders revealed that HPAHs might contribute additively to the PAHs-associated health risk to humans, indicating that more research is needed.

Levels of polycyclic aromatic hydrocarbons in milk and milk powder samples and their likely risk assessment in Iranian population

An MSPE/GC–MS method was used for the measuring of 16 PAHs and assessing of the effect of different factors on PAHs concentrations among different types of dairy products. Moreover, the probabilistic health risk assessment due to ingestion of PAHs by the consumption of milk and milk powder was evaluated. The limit of detection (LOD) and limit of quantitation LOQ were ranged between 0.040-0.075 and 0.121-0.227 μg/kg, respectively. The highest mean of total PAHs was noted in milk powdered (2.28 ± 0.39 μg/kg), while the lowest content was observed in pasteurized milk (0.87 ± 0.18 μg/kg). Except for a few samples of milk powder, the PAHs contents of the other samples was lower than standard limits while the concentration of BaP was lower than of standard levels proposed by EU (0.02-0.06 μg/kg). Considering season effect, the samples in winter had the highest level of PAHs. The percentile 95% actual THQ was the 3.64E-04 value that was lower than 1 value. Hence, the consumers are not at considerable non-carcinogenic health risk while the actual ILCR was 3.53E-03 as higher than 1E-04. Therefore, consumers are at considerable carcinogenic risk. Generally speaking, approaching the control plans for a decrease in the concentration of PAHs in dairy products in order to control carcinogenic health risk is crucial.

PAHs contamination levels in the breast milk of Ghanaian women from an e-waste recycling site and a residential area

Breast milk samples from 128 primipararae and multiparae Ghanaian women were screened for Polycyclic Aromatic Hydrocarbons (PAHs) from 2014 and 2016. These were breast milk from women residing or working around an electronic waste recycling site and a reference area (a residential area). This research is aimed at assessing PAHs levels in human milk samples from some Ghanaian mothers, prediction of the sources of these PAHs and the probable carcinogenic and mutagenic risks to infants. PAHs in the breast milk were analyzed using a gas chromatography coupled with a mass spectrometer (GC–MS/MS). A total of 18 PAH congeners were identified in the human milk samples with a total range between <LOD and 15,936.57 ng/g lipid wt and an overall mean of 1105.63 ng/g lipid wt. In general, the mean concentrations of low molecular weight PAHs were appreciably greater than the high molecular weight PAHs in the breast milk samples. Naphthalene recorded the highest mean concentrations of 1026.52 ng/g lipid wt and 78.73 ng/g lipid wt for both e-waste and residential areas, respectively. Naphthalene contributed 77.4% of the total PAHs in the milk samples. Most of the high molecular weight PAHs were below the limit of detection in milk samples from Kwabenya (a residential area) but were detected in the milk samples from Agbogbloshie (e-waste recycling site). The diagnostic ratio tests in this study suggest that most of the PAHs in the milk samples are originating from pyrogenic sources. Risk assessment for carcinogenicity and mutagenicity on infants based on this study were 1.1 × 10−5 and 1.9 × 10−5, respectively.

Polycyclic aromatic hydrocarbons in milk and dairy products

Polycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds with a structure consisting of carbon and hydrogen atoms that have more than two fused aromatic rings. Most PAHs have carcinogenic effects on animal or humans and induce various cancers. Polycyclic aromatic hydrocarbons are a consequence of environmental pollutants, imperfect burning or pyrolysis of organic substances during industrial processing. Additionally, foods can be contaminated during their processing and preparation through different heat treatments. This review highlights the formation, occurrence and health impacts of PAHs in milk and milk products, and analytical technique used for their determination in dairy products.

Study on the occurrence of polycyclic aromatic hydrocarbons in milk and meat/fish based baby food available in Italy

The study compared the profile of 14 polycyclic aromatic hydrocarbons, frequently occurred in food, in milk (N = 22) and meat/fish based (N = 18) baby foods available on the Italian market. PAH total levels, markers (Regulation EC/835/2011) and carcinogenic PAHs were determined by high- performance liquid chromatography with fluorescence detector (HPLC-FD). The average of total PAHs was 52.25 μg kg−1 in milk and 11.82 μg kg−1 in meat/fish based baby foods. The levels of PAH markers were higher than the permissible EU limits of 1 μg kg−1 in 18.2% and 77.7% milk, and 5.6% and 44.4% meat/fish based baby foods. Milk based samples showed significant higher values (P < 0.05) of carcinogenic and possible carcinogenic hydrocarbons than meat/fish based products. The Margins of Exposure (MOE) value of milk based baby food samples indicated a potential concern for consumer health. Monitoring programs, and good agriculture and manufacture practices should be recommended.

Presence of PAHs in milk of industrial farms from Tizayuca, Hidalgo, Mexico

The objective of this study was to determine the concentrations of polycyclic aromatic hydrocarbons (PAHs) in cow's milk from industrial farms that are located near an industrial park in Hidalgo, Mexico. It was found that the concentrations of PAHs in the raw milk of cattle from industrial farms have increased in recent years. Composite samples were collected between 2008 and 2010 and analysis carried out according to 8100 EPA procedures and analyzed by gas chromatography with FID detection. The results show that combustion PAHs were mostly Ace, Acy, and Fla (0.25, 0.32, and 0.22 µg g−1, respectively). Diagnostic ratios were used to show that the probable sources were grass and fuel combustion. The sum of concentrations of 16 individual PAHs did not breach permissible levels in milk (25 µg g−1 according to the United States EPA), indicating a limited health risk to animals and humans in the study area. The industrial park has adequate pollutant emission regulations.

Determination of polycyclic aromatic hydrocarbon metabolites in milk by a quick, easy, cheap, effective, rugged and safe extraction and capillary electrophoresis

We describe the first application of a quick, easy, cheap, effective, rugged and safe extraction technique to the CZE analysis of monohydroxylated metabolites of polycyclic aromatic hydrocarbons in milk. Complete resolution of 2-hydroxyfluorenene, 1-hydroxynaphthalene, 2-hydroxynaphthalene, 3-hydroxyphenanthrene, and 9-hydroxyphenanthrene was accomplished in 4 min of electrophoretic run. Limits of detection at the parts-per-billion were obtained with a single solvent (acetonitrile) for metabolite extraction and sample stacking. The small sample volume (1.2 mL) and the conservative usage of chemicals provided a simple and rapid procedure for the simultaneous extraction of numerous samples. Adding 4 min of electrophoretic run per sample, it should be possible to screen ten samples in approximately 1 h of analysis time. The nanoliter extract volume required for sample injection allows for further chromatographic usage and confirmation of positive samples. The unique electrophoretic pattern of the studied metabolites demonstrates the potential for the unambiguous determination of positional isomers with very similar chromatographic behaviors and undistinguishable mass fragmentation patterns.

Comparison of liquid–liquid extraction and solid-phase extraction for the determination of polycyclic aromatic hydrocarbons in the milk of Taiwan

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment and humans may be exposed to them through inhalation and ingestion. Most environmental hormones are carcinogens and can cause mutation. The study aims to quantify the content of PAHs in milk from various extraction solvents in different analysis methods. Random samples of milk produced in Taiwan and popular milk brands are the study objects and the collected milk is experimented in order to understand the main distribution and feature of PAHs all around Taiwan. Liquid–liquid extraction method is applied to check the content of PAHs. The result shows that four chemical compounds are found, i.e. NaP, AcP, Flu, and PA. Furthermore, toxic BaP is found in four samples of milk produced in Taiwan and two in popular milk brands. According to the factor analysis, the fall-point between the first and the second factor is very close and it means that the pollutant of PAHs could be from the same area or a close area. It shows that due to the dense population, the milk production center is possibly located in a busy traffic area and the exhaust emitted from vehicles could be the possible source.

Experimental design for the optimization of the extraction conditions of polycyclic aromatic hydrocarbons in milk with a novel diethoxydiphenylsilane solid-phase microextraction fiber

An innovative solid-phase microextraction coating based on the use of diethoxydiphenylsilane synthesized by sol–gel technology was used for the determination of polycyclic aromatic hydrocarbons at trace levels in milk. The effects of time and temperature of extraction and acetone addition were investigated by experimental design. Regression models and desirability functions were applied to find the experimental conditions providing the highest global extraction response. The capabilities of the developed fiber were proved obtaining limit of quantitation values in the low μg/l range, enabling the direct analysis of complex matrices like milk and a complete desorption of high-boiling compounds without carryover effects.

Determination of 16 polycyclic aromatic hydrocarbons in milk and related products using solid-phase microextraction coupled to gas chromatography–mass spectrometry

A method for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) in milk and related products based on direct immersion-solid phase microextraction (DI-SPME) followed by gas chromatography–mass spectrometry detection (GC–MS) has been developed. The influence of various parameters on PAH extraction efficiency was carefully monitored. Good performance (recovery, precision and quantitation limits) was attained when a PDMS/DVB fiber was immersed in the sample for 60 min at 55 °C. Detection limits ranged from 0.003 to 1.5 μg L −1 at a signal-to-noise ratio of 3, depending on the compound and the sample. The proposed method was successfully applied to infant formulas, milk and related products and the presence of both fluoranthene and pyrene in two samples was confirmed.

Short Communication: Study of Mammary Epithelial Role in Polycyclic Aromatic Hydrocarbons Transfer to Milk

Food would appear to be one of the main routes for animal and human contamination with polycyclic aromatic hydrocarbons (PAH). Many studies have shown the presence of PAH in milk and dairy products, suggesting that these foods can represent a part of this contamination. Our work aimed at defining, in vitro, the mammary barrier role in PAH transfer to milk. MAC T cells were cultivated on permeable filters to measure transepithelial permeability of 14C labeled benzo[a]pyrene (BaP), pyrene (Pyr), and phenanthrene (Phen), which differed in their physicochemical properties. The results showed that only 2 molecules, Phen and Pyr, were able to cross mammary cell layers. Phenanthrene radioactivity appeared more quickly in apical media, and its level after a 6-h exposure was 1.3 times higher than for Pyr and 7.7 times higher than for BaP. These findings suggested that mammary epithelium could play a key role in the selective transfer of PAH from food to milk.

Determination of Polycyclic Aromatic Hydrocarbons in Milk Commercially Available in Tokyo and in Raw Milk from Cattle Farm

Determination of Polycyclic Aromatic Hydrocarbons in Milk Commercially Available in Tokyo and in Raw Milk from Cattle Farm