Polyherbal Formulation Research Articles

Effect of a polyherbal Unani formulation on left ventricular diastolic dysfunction in hypertensive patients- a randomized single blind placebo controlled clinical trial.

Diastolic dysfunction represents an important pathophysiological intermediate between hypertension and heart failure. In the last two decades, the prevalence of heart failure patients having normal or near normal ejection fraction (EF) has increased to around 60 %. It thus poses a great morbidity and mortality risk to the population. In view of present scenario of high prevalence, lack of evidence-based therapy, and limited clinical trials, this study aimed to evaluate how a Unani formulation affects the improvement of the left ventricular diastolic function. This clinical trial was set up as a randomized, placebo-controlled study involving 35 participants, with 18 individuals in the test group and 17 in the control group. Test group received 3.5 g of a polyherbal Unani formulation in capsule form along with 35 mL of an extract of Borago officinalis L. (Arq-e-Gaozaban), divided into two doses after meals. Meanwhile, the control group received a placebo in the same manner over an eight-week period. Follow-ups were conducted every 15days to assess both subjective and objective parameters in all participants. The test formulation shows significant improvement in dyspnea and diastolic function from baseline to the end of trial (p<0.05), slight improvement in palpitations (p>0.05) and highly significant improvement in easy fatigability (p=0.001) as compared to the control. The present study shows the effectiveness of the test drug in enhancing the diastolic function of left ventricle and alleviating other symptoms associated with ventricular diastolic dysfunction. Nevertheless, additional research with longer follow-up durations is necessary to clarify its efficacy and establish optimal treatment approaches for ventricular diastolic dysfunction in Unani medicine.

Effect of Polyherbal Formulation on High Fat Diet Induced Obesity in Rats

Obesity is one of the major chronic diseases in which excess body fat accumulates in visceral organs. It is a chronic metabolic disease due to an imbalance between energy intake and expenditure. Various synthetic treatments are available in the market but have side effects such as Joint pain, chest tightness, tiredness, Back and stomach pain. The aim of study was to examine the anti-obesity potential of prepared polyherbal formulation (Hugonia Mystax: Blumea Lacera::1:1) in female Wistar rats. The obesity was induced by a high fat diet. Group 1 Placebo (normal control), Group 2-high fat diet (disease control), Group 3-high fat diet and orlistat (20 mg/kg for last 2 weeks, Group 4 treated with high fat diet and low dose elixir (200 mg/kg i.e. 4ml orally for last 2 weeks of study), Group 5- high fat diet and high dose elixir (400 mg/kg i.e. 8ml orally for last 2 weeks of study). At the end of study rats were subjected to evaluation of physical parameters, liver profile, serum blood glucose level and histopathology of liver and adipose tissue. The result states that formulation has the potential to work on various obesity mechanisms especially on energy intake. The result of the present study concluded that the prepared formulation has anti-obesity potential.

In vitro antioxidant and anti-inflammatory activities of selected polyherbal formulations sold in Nigeria

Background and aims: Nonsteroidal anti-inflammatory drugs (NSAIDs) or corticosteroid analgesics are recommended to alleviate or reduce pain. Regrettably, many of these drugs may induce bleeding, dyspepsia, cardiac complications, renal issues, and various short- or long-term adverse effects. Consequently, there has been an escalation in the endeavor to develop natural anti-inflammatory medications, driven by the growing number of individuals seeking natural therapies to manage their pain. The polyherbal formulations (Yoyo and Dr. Iguedo Goko bitters) have not assessed their antioxidant and anti-inflammatory activities; hence, this study "in vitro antioxidant and anti-inflammatory activities of selected polyherbal formulations sold in Nigeria." Methods: The evaluation of antioxidant activity was performed using three assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, hydrogen peroxide (H2 O2 ) scavenging activity, and ferric reducing antioxidant power. In vitro, anti-inflammatory efficacy was investigated using the human red blood cell (HRBC) membrane stabilization technique. Results: The DPPH radical scavenging ability result was observed; the IC50 value of Yoyo bitters (40.63±0.90 % at 250 µg/mL) was highest, while Dr. Goko bitters (3.26±0.21% at 50 µg/mL) was lowest. Meanwhile, the positive control (ascorbic acid) had an IC50 value of 93.54±0.57% at 250 µg/mL. The ferric-reducing antioxidant power of the samples was highest in Dr. Goko bitters, 1.96±0.02 % at 250 µg/mL, and lowest in Dr. Goko and Yoyo bitters, 0.46±0.01% at 100 and 50 µg/mL, respectively. The hydrogen peroxide free radical scavenging activity of the samples was highest in Yoyo bitters, 58.0.3±0.60 % at 250 µg/mL, and lowest in Dr. Goko bitters, 7.28±0.02 % at 50 µg/mL. The anti-inflammatory findings indicate that Yoyo bitters exhibited the highest percentage protection (89.46±6.11%) at a concentration of 1000 μg/mL, while the lowest percentage protection (26.62±1.13%) was observed at a concentration of 200 μg/mL. The reference standard diclofenac sodium has a percentage of 90.46±1.44 at a concentration of 1000 μg/mL. Meanwhile, the poly-herbal formulations had the highest and lowest total phenolic concentrations in Yoyo bitters (97.52±1.43 mg GAE/g at 250 µg/mL), and Dr. Goko bitters (47.3±1.44 mg GAE/g at 50 µg/mL), respectively. Conclusion: The findings suggest that the formulations might not have effective antioxidant and anti-inflammatory activities when compared with the reference standard drugs (ascorbic acid and diclofenac sodium), respectively.

Analytical method development and validation for simultaneous estimation of seven markers in polyherbal formulation JKC by using RP-HPLC

BackgroundThe RP-HPLC method has been established to simultaneous estimation of seven markers in polyherbal formulation JKC using the C18 (25 × 0.46 cm, i.d,5 µm) column. The mobile phase consisted of methanol: water (80:20) at a flow rate of 1.0 mL/min and observed retention time at 2 to 11 min with sharp points. The marker compounds viz. Andrographolide (AG), Piperine (PP), Picroside-I (P-I), Picroside-II (P-II), α-Cyprone (AC), 6-Shogaol (6S), and 6-Gingerol (6G) were quantified in JKC formulations by HPLC method. Detection was performed at the wavelength (λ) of 229 nm for AG, 343 nm for PP, 279 nm for P-I, 264 nm for P-II, 254 nm for AC, and 280 nm for both 6S and 6G by HPLC–PDA detector.ResultsThe marker compounds in JKC formulations were observed in different retention times (Rt) i.e. AG at 3.060 ± 0.01 min, PP at 5.460 ± 0.03 min, P-I at 2.789 ± 0.02 min, P-II at 2.553 ± 0.03 min, AC at 10.951 ± 0.02 min, 6S at 6.302 ± 0.03 min, and 6G at 4.111 ± 0.02 min respectively. The proposed method was validated with acceptable linearity (r2 0.9995–0.9999), precision, robustness, ruggedness, and accuracy (RSD < 2%) under optimum conditions. The limit of detection and quantification of bioactive markers were as: AG (1.386; 4.200 ppm), PP (2.033; 6.161 ppm), P-I (2.822; 8.553 ppm), P-II (2.538; 7.691 ppm), AC (0.269; 0.815 ppm), 6G (0.158; 0.480 ppm), 6S (0.188; 0.569 ppm). The amount (mg/g) of bioactive markers detected and estimated in plants and formulation were as: AG (41.282 ± 0.48; 10.06 ± 0.18), PP (53.81 ± 0.25, 13.82 ± 0.37 in PN, PL; 4.27 ± 0.07), P-I (15.97 ± 0.01; 0.48 ± 0.003), P-II (63.24 ± 0.35; 2.31 ± 0.006), AC (0.42 ± 0.01; 0.36 ± 0.006), 6G (0.71 ± 0.03; 0.16 ± 0.001), and 6S (2.64 ± 0.09; 0.12 ± 0.004) respectively. Method was found to be rugged and robust. The results found for all the validation parameters were within the limits according to ICH guidelines.ConclusionThe proposed method is fast, precise, economic, and specific and used for the simultaneously quantifiable analysis of seven major bioactive markers in the ingredients (herbs) and the JKC formulations.

Formulation and Evaluation of Diuretic Activity of Polyherbal Drug in Rats

An attempt was made in this study to investigate the diuretic activity of an ethanolic extract of a polyherbal formulation containing four drugs: seeds of Coriandrum sativum, buds of Syzigiumaromaticum, leaves of Ocimum sanctum, and curcumin (Curcuma longa), as well as leaves of Syzigiumaromaticum. According to the findings of this investigation, the extract of a polyherbal formulation (including seeds of Coriandrum sativum, buds and leaves of Syzigiumaromaticum&amp; leaves of Ocimum sanctum, and Curcuma longa) possessed substantial activity. Various quantities of Polyherbal formulations (200 and 400 mg/kg), furosemide (10 mg/kg), and vehicle were given orally to rats (n = 6 animals per group), and the urine output was collected after 24 hours. All concentrations of Polyherbal formulation exhibited a dose-dependent relationship when compared to the control animals in the study. According to the findings of this study, the Polyherbal formulations have significant diuretic effect in rats when tested in the above-mentioned experimental model. That polyherbal formulation extract has such potent effect may be due to their ability to stimulate the excretion of Na+, K+, and Cl- concentrations in urine while also increasing the amount of urine excreted by the body. As diuretics, medicinal herbs are a key source of supply. When it comes to diuretics, both mono- and poly-herbal formulations have been employed successfully

Assessment of Plants Used in Polyherbal Formulations for Traditional Treatment of Skin Infections in Ibadan Metropolis

IntroductionThere are numerous skin diseases and frequently occurring health problems that affect all ages, from neonates to the elderly, and in many ways cause harm. Natural treatment is cheap and claimed to be safe. It is also suitable for raw materials or the production of new synthetic drugs.The present study was aimed at carrying out an ethnobotanical investigation of polyherbal formulations traditionally used for the treatment of skin infections in the Ibadan metropolis. MethodsA comprehensive ethnobotanical survey was carried out in six local government areas within the Ibadan metropolis of Oyo State, Nigeria, where a substantial number of respondents were administered questionnaires. Information on the respondents' socioeconomic status, the types of plants utilised in polyherbal medicine, and the preparation and skill-acquisition techniques of the local herbal practitioners were all gathered. The generated data were subjected to descriptive statistics analysis. ResultsThe obtained results showed that Daniella oliveri, Olax subcorpioidea, Khaya grandifolia, Piper guineensis, Anogesius leocarpa, and Alstonia boonei are the most often utilised herbs for treating skin infections. Barks are mainly used because the highest occurring mode of preparation recorded was decoction, and barks are mostly decocted to release the active constituents in them for the treatment of fungal skin infections. The most common method of administration of the herbal preparations was drinking the decoction after preparation. ConclusionsThis study confirmed that polyherbal medicines have great potential in treating various types of skin infections and a few rural and urban dwellers still depend on traditional healing methods.

Sub-chronic Hepatotoxicity Assessment of Ghana Cleanser® in Exposed Wistar Rats

This study evaluated the toxicity of a polyherbal Formulation (Ghana Cleanser®) on liver function markers of exposed albino rats. Thirty (30) male and female rats of the Wistar strain were randomly allotted into six (6) groups with n=5. 10.0 mL/kg distilled water was given to control groups 1 and 4. Polyherbal formulation doses of 374.0 mg/kg and 187.0 mg/kg were administered to groups 2-3 and 5-6, respectively. A modified Lorke's approach was used to compute acute toxicity. Animals were euthanized after 60 days under diethyl ether. Blood was collected for biochemical analyses through cardiac puncture. The liver was excised from each animal and was fixed in 10% buffered formaldehyde and prepared for histological assessment. LD50 of the polyherbal preparation was calculated as 3740 mg/kg (oral). The results indicated an appreciable increase (p&lt;0.05) in ALT activity at 374.0 mg/kg in female rats; while there was no increase recorded at 187.0mg/kg in male rats. A significant increase in ALT activity was recorded at 374 mg/kg in male rats as well and increased AST activities were recorded at 187mg/kg in female rats. In the treated animals of both sexes, ALP activities were significantly elevated. Histopathology assessment of the hepatocytes showed no significant damage at 187 mg/kg in rats of both sexes when compared with their respective controls while some degrees of pathologies such as hepatocyte inflammation, hyperplasia, and congestion were recorded at 374 mg/kg in rats of both sexes. Results suggest caution on the long-term use of the polyherbal mixture due to its hepatotoxic potential.

Formulation and Evaluation of Polyherbal Antidiabetic Tablet

About 60% of people use traditional medicines that are made from medicinal plants. This article focuses on Indian herbal medicines and plants that are utilized, particularly in India, to treat diabetes. Diabetes is a serious illness that affects many people worldwide from all areas of life. It is proven to be a serious health issue in India, particularly in the cities. Although there are many ways to lessen diabetes' negative effects and its secondary complications, herbal formulations are preferred because they have fewer side effects and are less expensive. A list of medicinal plants having established antidiabetic properties, as well as herbal medications used to treat diabetes, is compiled. These includes, Tinospora cordifolia, Azadirachta indica, Enicostemma axillare, Momordica Charantia, Phyllanthus emblica, Centratherum anthelminticum. Damage caused by free radicals is one of the etiologic factors linked to the onset of diabetes and its complications, so an anti-diabetic drug having antioxidant qualities would be more advantageous. Historically, substances and extracts obtained from various natural resources, particularly plants, have been a valuable tool for managing and treating diabetes-related issues and complications. Therefore, this review aids the reader in understanding the significance of various herbal and polyherbal formulations that have been used traditionally to treat diabetes mellitus.

Formulation Development and Evaluation of Polyherbal Emulgel for Anti Inflammatory Activity

This study focuses on the formulation development and evaluation of a polyherbal emulgel incorporating curcumin and coriander seed oil, aimed at enhancing anti-inflammatory activity. Preformulation studies were conducted to characterize the physical, chemical, and mechanical properties of the active substances, and to establish a robust basis for the formulation approach. Various parameters including solubility, melting point, partition coefficient, and drug-excipient interactions were assessed to ensure compatibility and efficacy. The emulgel was prepared through a systematic process involving the preparation of a gel base, oil phase, aqueous phase, and subsequent emulsification. The final product was evaluated for various properties such as physical appearance, homogeneity, pH, spreadability, extrudability, swelling index, viscosity, drug content, and in vitro drug diffusion. Stability studies were also conducted over three months to ensure the longevity and reliability of the formulation. Results indicated that the formulated emulgel exhibited desirable physical properties, optimal pH, high spreadability, good extrudability, and a significant swelling index. Viscosity measurements confirmed appropriate consistency, while drug content analysis ensured accurate dosing. In vitro drug release studies demonstrated sustained release profiles, fitting well with kinetic models. Additionally, in vivo anti-inflammatory activity was assessed using a carrageenan-induced paw edema model in rats, showing promising results with notable inhibition of inflammation. Overall, the polyherbal emulgel formulation developed in this study offers a promising alternative for anti-inflammatory therapy, combining the therapeutic benefits of curcumin and coriander seed oil in a stable and effective dosage form. Further studies are recommended to explore clinical efficacy and potential applications in broader therapeutic areas

A PHARMACOLOGICAL REVIEW OF PUTIKADI LEPA FOR DADRU

The reported prevalence of fungal infection is about 40 million people in developing and underdeveloped countries. The incidence of fungal infection is found to be more during summer and rainy seasons. Local Dermatophytosis / tinea infection i.e. severe itching and annular erythematous scaly patches are the symptoms.As per classical texts, the clinical expression of Tinea infection can be closely related with Dadru. Acharya Charak has explained it in Kshudra Kushtha while Acharya Sushruta in Mahakushtha. Its symptoms include Kandu(itching), Raga(redness), Atasipushpvat Pidika (macular rashes similar to flax flower). For the management of Dadru, Putikadi Lepa is an effective poly herbal formulation. It consists of Putik, Ark, Aragvadh, Snuhi, Jati and its local application with Gomutra. It has Kusthagna, Kandughna properties. Many research studies have demonstrated that these drugs possess antifungal, antimicrobial, anti-bacterial properties. The Gomutra used for the application of Putikadi Lepa contains various elements which hydrates the skin and improves the activity of Lepa. Thus, this article deals with the pharmacological action of the ingredients of Putikadi Lepa.

Pharmacognostic, Phytochemical and Antimicrobial Study of Vidangadi Churna - An Experimental Study

Herbal medicines are gaining more and more attention all over the world as they have been in use since ancient times and have no side effects if taken under proper supervision. But they have their own limitations as data about standardization and quality control are not widely available. Therefore, it is the need of the hour to revalidate this herbal compound under the lens of advance tools or procedures. Method: Vidangadi Churna was prepared according to the classical reference. Physiochemical and Phytochemical study was performed by proper WHO guidelines and Antimicrobial activity of Vidangadi Churna was tested by Agar well diffusion method. Result and Discussion: In this study Vidangadi Churna showed the presence of phytochemical constituents having antimicrobial properties. This preparation was treated with different reagents and examined under UV light. Different reagents showed different colours of the preparation at different wavelengths. Various physiological active compounds like phenolic compounds, Tannins, Amino acids, alkaloids etc. were found in Vidangadi Churna. Antimicrobial study revealed the activity of this drug on different micro-organisms. Conclusion: Vidangadi Churna is poly-herbal Ayurveda formulation described in Yogratnakar in Kustha Roga Chikitsa Adhyay. By this study it is concluded that Vidangadi churna fulfilled all the standard criteria with effective parameters and it could be used as anti-microbial agent against infections caused by Klebsiella aerogenes, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

Assessment of polyherbal formulations in managing diabetic retinopathy: An in-vivo experimental model study

Assessment of polyherbal formulations in managing diabetic retinopathy: An in-vivo experimental model study

Pharmacognostical and Pharmaceutical Analysis of Yavanishadavadi Vati - An Ayurvedic Polyherbal Formulation

Pulmonary Tuberculosis (PTB) is caused by the aerobic acid-fast rod-shaped bacterium Mycobacterium tuberculosis. This tiny bacterium, expelled from a contagious host, can remain suspended in air as droplet nuclei for hours. Multidrug-resistant tuberculosis (MDR-TB) is a form of TB caused by bacteria that do not respond to isoniazid and rifampicin, the 2 most effective first-line TB drugs. In Ayurveda, the term Rajayakshma has been used interchangeably with tuberculosis. Rajayakshma is primarily attributable to Dhatukshaya (tissue emaciation or loss). Aim: To analyze the Pharmacognostical and Pharmaceutical properties of Yavanishadavadi Vati. Materials and Methods: Yavanishadavadi Vati was subjected to microscopic evaluation for pharmacognostical study, physico-chemical analysis like hardness, weight variation, loss on drying, ash value, acid insoluble extract, pH value, water soluble extract, alcohol soluble extract and high performance thin layer chromatography (HPTLC). Result: Pharmacognostical study showed the presence of certain identifying characteristics of all the ingredients of Yavanishadavadi Vati that is Yavani, Tintidaka, Shunthi, Amalvetasa, Dadima, Badara, Dhanyaka, Suvarchal Lavana, Jeeraka, Dalchini, Pippali, Maricha and Sarkara. In pharmaceutical study, preliminary physico-chemical analysis showed that hardness of Yavanishadavadi Vati was hardness 6.6Kg/cm2, ash value 10.59%w/w, loss on drying 8.17%w/w, water soluble extract 28.6%w/w, alcohol soluble extract 20.2%w/w and pH value was 6.5. HPTLC analysis showed eleven spots in 254nm and nine spots in 365nm. Conclusion: Present work was carried out to standardize the polyherbal formulation Yavanishadavadi Vati in terms of its identity, quality and purity. Pharmacognostical and physico-chemical observations revealed the specific characters of all the active constituents in the preparation.

Integrative network pharmacology, molecular docking, and dynamic simulation analysis of a polyherbal formulation for potential therapeutic impact on prostate cancer

BackgroundProstate cancer (PCa) remains a significant health concern globally, prompting a continual search for novel therapeutic strategies. In this study, we employed a comprehensive approach combining network pharmacology, molecular docking and dynamic simulation to explore the potential impact of a polyherbal formulation on PCa. MethodsUtilizing comprehensive network pharmacology approaches, we elucidated the complex interactions between the bioactive compounds within the polyherbal formulation and key targets associated with PCa progression, highlighting their multitarget mechanisms through integrated protein‒protein interaction and KEGG pathway analyses. Molecular docking simulation studies were performed to predict the binding affinities and modes of interaction between the identified bioactive compounds and their respective protein targets. ResultsComplex connections comprising 486 nodes and 845 edges were found by the compound-target network analysis. Significant interactions were observed, and the average node degree was 4.23. KEGG research revealed that PCa and the PI3K-Akt signalling pathway are implicated in modulating prostate cancer. The Quercetin docking investigations revealed that the binding energies for AR and PIK3R1 were −9 and −9.5 kcal/mol, respectively. Based on the results of the MD simulations, it appears that tiny molecules and proteins have formed stable complexes with low fluctuations. ConclusionIn conclusion, this comprehensive method emphasises the value of network pharmacology in conjunction with molecular docking and dynamic simulation in revealing the anti-PCa therapeutic potential of polyherbal formulations, opening up new possibilities for the creation of efficient anti-cancer medicines.

Standardization and evaluation of the antiulcer potential of a traditional polyherbal formulation in experimental animals

Standardization and evaluation of the antiulcer potential of a traditional polyherbal formulation in experimental animals

Assessment of Proximate, Vitamins, In Vitro Antioxidant, and Functional Properties of Some Polyherbal Formulations as Prospective Botanical Candidates for Pharmaceutical Applications

Study’s Novelty/Excerpt This study evaluates the combined effects of Turmeric, Coriander, and Mint (TCM) leaves on nutritional, functional, and antioxidant properties in polyherbal formulations, showing their superior glucose absorption capacity and antioxidant activity compared to single herbs. The research highlights a significant nutritional shift in the TCM formulation, providing evidence for its enhanced efficacy in nutraceutical applications. These findings show the potential of TCM as a novel botanical candidate for safe and effective pharmaceutical development. Full Abstract Conventional drugs have numerous side effects, toxicity, and microbial resistance, making them unsafe for consumption. Polyherbal formulations are popular due to their potency, low cost, clinical efficacy, safety, and success in chronic conditions. The study aims to evaluate the proximate, vitamin, functional, and antioxidant activity of polyherbal formulations of Turmeric, Coriander, and mint (TCM) leaves as potential botanical candidates for pharmaceutical screening. Thus, addressing these issues and improving access to drugs for individuals by focusing on polyherbal formulations (PHF) is a potential solution. All analyses conducted were determined according to standard assay guidelines. The proximate analysis shows no significant difference among the formulations (p &gt; 0.05), with TCM showing a nutritional profile shift because of the combination of the herbs rather than single herbs. In the vitamin composition, Vitamin A displays a pattern among the formulations, with TC and CM showing higher values than TM and TCM, which are, in turn, better than the single herbs. Vitamin C fluctuates across the formulations. TM displays higher, while T shows the lowest. Vitamin E content remains relatively stable among the formulations with a difference from the single herbs. In the functional properties, GAC displays notable variations among the formulations. TCM exhibits the highest GAC at 14.7 %, indicating its superior ability to absorb glucose. TC, CM, TM, and the single herbs show a comparatively lower GAC value. WAC also varies among the formulations. TCM displays lower WAC compared to TC, TM and CM. The single herbs show the lowest WAC. OAC shows intriguing trends across the formulations. TCM and TC exhibit similar OAC, while CM shows a slightly higher OAC, and TM displays better than single herbs, showing no significant difference (p &gt; 0.05). In the antioxidant Activity, TCM shows lightly higher scavenging activity in 40, 80 and 120 mg/mL concentrations than other formulations. The outcome of this study has led us to conclude that employing safe combinations of these herbal formulations for the production of nutraceuticals is recommended.

Development and evaluation of poly herbal powder formulation of energy booster powder

Development and evaluation of poly herbal powder formulation of energy booster powder

Anti-oxidant and Anti-bacterial activity of polyherbal formulation

Anti-oxidant and Anti-bacterial activity of polyherbal formulation

Anti-inflammatory effect of Polyherbal Formulation

Inflammation is body’s defence mechanism. There are various inflammatory and pro-inflammatory factors released in blood after injury. Prevalence of diseases associate with chronic inflammation will increase persistently in next 30 years. 3 in 5 people died with inflammation. Currently various synthetic treatments available but have many side effects. In this view, present study was designed to prepare a promising anti-inflammatory treatment from natural source. Anti-inflammatory effect of polyherbal formulation [prepared from ethanolic extracts of Hugonia mystax and Blumea lacera (1:1)] was evaluated against carrageenan and formalin induced paw oedema. Preliminary phytochemical screening revealed that phenolic acids, flavonoids, tannins, present in formulation. Acute oral toxicity was performed as per OECD guideline, 200 mg/kg and 400mg/kg was selected as lower dose and higher dose respectively. Paw oedema and paw thickness were assessed in all groups. Paw oedema and paw thickness increase in disease control group and significantly decrease in all treatment groups. It is concluded that polyherbal formulation have anti-inflammatory potential due to polyphenols, saponin and terpenoids. Further potent compounds can be isolated for preparation of formulation and clinical trials may be performed.

A Review of Traditional Anthelmintic Approaches: Exploring Medicinal Plants and Polyherbal Formulations for Effective and Safe Gastrointestinal Parasite Treatment

Abstract: Gastrointestinal parasites have become the most common reason for infection in humans, affecting many people in the world. Helminthic infections pose a large threat to public health in developing countries. The most common parasites of helminths in humans are whipworms(Trichuristrichiura), roundworms(Ascarislumbricoides), tapeworms(Taenia solium), and hookworms (Ancylostoma duodenale and Necator americanus). In the current scenario, synthetic medicines are found to be effective in curing the infection but these medicines produce side-effects that impair the effectiveness of the treatment. Fortunately, in the traditional system of medicines various medicinal plants have been mentioned for their anthelmintic activities, which are relatively free from side effects and are used by ethnic groups worldwide. In the 21st century, an ideal anthelmintic agent should be cost-effective, provide a high percentage of cure, have a broad spectrum of action, and free from toxicity to the host. However, none of the modern marketed medicines fulfill these requirements. Therefore, in this view, several researchers have undertaken various in vivo and in vitro studies to scrutinize folklore medicinal plants for their proclaimed anthelmintic activity. In thisreview, we have summarized some scientifically approved polyherbal formulations and Indian medicinal plants documented for anthelmintic activity, which can be further studied to achieve novel herbal lead molecules from them by isolating their principal compounds.